eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2020-09-25 13 3 1157 1168 10.13005/bpj/1983 35047 article Tilak Ram Chandrakar 1 Ajit Pratap Singh 2 Bikas Chandra Sarkhel 2 Suvendra Nath Bagchi 1 Department of Biological Science, Rani Durgavati University, Jabalpur 482001, Madhya Pradesh, India. Animal Biotechnology Center, Nanaji Deshmukh Veterinary Science University, Jabalpur 482001, Madhya Pradesh, India. Pesticides constitute a different class of chemicals, basically designed for the protection of agricultural crops by controlling a variety of insects, pests, weeds harmful for the agricultural plants. Pesticides are considered as potential chemical mutagens. Experimental data have shown that various agrochemicals have lethal effects, including mutational properties such as chromosomal changes, DNA damage, micronuclei formation or cytotoxicity in the cellular level. This study was designed to examine the cytotoxic and genotoxic effects of carbofuran and malathion pesticides on cat (Felis catus) fibroblast cells. The deterrent effects were assessed based on cell viability, chromosomal changes and DNA damage on fibroblast cells in approx. 1×10⁵ cells. The cells were exposed to 0.045 mM - 1.08 mM of carbofuran for 24 h, and 5 mM – 45 mM of malathion for 48 h. The effects were evaluated in terms of DNA damage as changes in comet tail length, comet scores, chromosomal aberrations and micronucleus formation. Lethal doses were determined by using MTT assay whereas crystal violet staining was carried out to assess cytotoxic effects. The LC₅₀ concentrations of carbofuran and malathion were estimated to be 0.42 mM and 20 mM, respectively. The maximum DNA damage was attained at 1.08 mM of carbofuran and 45 mM of malathion. The genotoxic and cytotoxic effects of the two chemically different pesticides in F. catus fibroblast cells was evident. While carbofuran was more toxic in terms of lethal doses and exposure time, malathion seemingly promoted genomic instability, albeit at unusually high concentrations. https://biomedpharmajournal.org/vol13no3/in-vitro-cytotoxicity-and-genotoxicity-assessments-of-carbofuran-and-malathion-pesticides-on-cat-felis-catus-fibroblast-cells/ Carbofuran Cytotoxicity Felis Catus Fibroblast Cell Line Genotoxicity Malathion