eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2020-09-25 13 3 1169 1174 10.13005/bpj/1984 34906 article Clinacanthus nutans is widely used as an antimicrobial, anti-inflammatory, anti-pyretic and cytotoxic agent in Asian countries such as Thailand and Malaysia. Our previous study suggested C. nutans may exhibit anti-inflammatory effects. However, there are limited studies at current for the antimicrobial affect for this plant; hence this study was carried out to investigate the minimum inhibitory concentration (MIC) of C. nutans polar and non-polar leave and stem extracts against twelve gram- negative and positive bacteria. Briefly, the plant parts were separated, dried and powdered, then the extracts were prepared as polar and non-polar extracts from leaves and stem. The result showed inhibition effects in all of the bacteria tested. Our findings from this study indicated that non-polar leaves extracts are more potent as an antimicrobial agent compared to polar leaves, non-polar stem and polar stem. Future studies are warranted for further identification of bioactive compounds in C. nutans with anti-bacterial activities. https://biomedpharmajournal.org/vol13no3/antibacterial-activity-of-clinacanthus-nutans-polar-and-non-polar-leaves-and-stem-extracts/ Antibacterial Antimicrobial activity  Anti-inflammator Bacterial resistance  Clinacanthus nutans  Natural product.