eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2020-06-25 13 2 551 554 10.13005/bpj/1917 33929 article Wayne Wen-Yan Xu 1 Jun Yuan 1 Weilun Gong 1 Guohao Xie 1 H. Fai Poon 1 Jiaojiao Zhang 2,3 Quacell Biotechnology Co., Ltd., No. 6 Shengnong Road, Zhongshan, 528400, China Dermatology Hospital, Southern Medical University, No.2 Lujing Road, Guangzhou 510095, China Department of Clinical Sciences, Faculty of Medicine, Polytechnic University of Marche, Ancona, 60131, Ancona, Italy Cell culture media has been one of the key factors in cell culture technology since media supports cell survival and proliferation, as well as cellular functions. The quality and characteristics of a medium directly impact the research experimental results, the biopharmaceutical production rate, even the treatment outcomes of patients. Here we summarized the chronological development of balanced saline solution, natural media, synthetic media, classical media, serum free media, chemically define media, industrial media, protein free media, and animal component free media. This review has provided the information needed to facilitate investigators to decide an appropriate medium according to their aims. https://biomedpharmajournal.org/vol13no2/cell-culture-media-chronicle/ Chronological Development Classical Media Natural Media Synthetic Media Serum Free Media