eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2020-06-25 13 2 873 881 10.13005/bpj/1953 33952 article Ni Putu Linda Laksmiani 1 Luh Putu Febryana Larasanty 1 Anak Agung Gde Jaya Santika 1 Putu Agus Andika Prayoga 1 Anak Agung Intan Kharisma Dewi 1 Ni Putu Ayu Kristiara Dewi 1 Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Udayana University, Iodonesia , 80362 Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), better known as the coronavirus, is a new type of coronavirus that is transmitted to humans. This virus infection is called COVID-19 and was first discovered in the city of Wuhan, China, at the end of December 2019. This virus spread quickly and has spread to other regions in China and several countries, including Indonesia. This disease results in coronavirus pandemic 2019-2020. The objective of this research is to determine the inhibitory ability of several active compounds from natural sources against COVID-19 target protein in silico using molecular docking. In silico research was conducted using autodock 4.2 program by evaluating the binding energy between the active compound with ACE2, TMPRSS2, RdRp, 3CLpro and PLpro as the target proteins. All chemical compounds that evaluated such as asiatic acid, andrographolide, apigenin, brazilein, brazilin, catechin, curcumin, gingerol, hesperidin, hesperetin, kaemferol, luteolin, myricetin, naringenin and quercetin had an affinity to target protein. It reflects that active compounds in medicinal plants can be used as antiviral against COVID-19. Brazilein and brazilin from secang wood (Caesalpinia sappan L.) have a superior bond to ACE2 and lower binding energy value than chloroquine, arbidol, remdesivir, ribavirin and lopinavir. Citrus sp containing hesperidin had an excellent affinity to TMPRSS2. Secang wood and citrus sp. could be developed as an anti-SARS-CoV-2 through inhibiting ACE2, TMPRSS2, RdRp and protease (3CLpro and PLpro) that interfered the process of virus infection at the entry, replication and advanced stages, causing worst effect such as pneumonia. https://biomedpharmajournal.org/vol13no2/active-compounds-activity-from-the-medicinal-plants-against-sars-cov-2-using-in-silico-assay/ ACE2 Citrus sp Protease RdRp Secang wood (Caesalpinia sappan L.) TMPRSS2