eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2020-03-28 13 1 441 448 10.13005/bpj/1904 31255 article Kailas D. Datkhile 1 Satish R. Patil 1 Pratik P Durgavale 1 Madhavi N. Patil 1 Nilam J. Jagdale 1 Vinit N. Deshmukh 1 Molecular and Genetic Laboratory, Department of Molecular Biology and Genetics, Krishna Institute of Medical Sciences “Deemed to be University”, Taluka-Karad, Dist-Satara, Pin-415 539, Maharashtra, India Biogenic nanoparticles have emerged as novel contender in biomedicine because of their comprehensive tendency and remarkable potential in therapeutics. This study was intended to synthesize biogenic silver nanoparticles using Nothapodytes foetida leaf extract (Wight) (NFAgNPs). The biosynthesis of silver nanoparticles was continuously monitored by time dependent UV-visible spectrophotometric analysis. The morphology, size and shape of biogenic NFAgNPs were studied by transmission electron microscopy (TEM). In vitro antioxidant potential of biogenic nanoparticles evaluated by assaying percentage inhibition of 2, 2’-diphenyl-1-picrylhydrazyl (DPPH) and 2, 2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radicals. Antimicrobial activity of NFAgNPs was judged by minimum inhibitory concentration (MIC) assay on Gram-positive (Staphylococcus aureus) and Gram- negative (Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae bacterial strains. The results confirmed that the NFAgNPs exhibited strong antioxidant potential where more than 93.80 % of inhibition in DPPH radicals occurred at 100 µg/mL concentration NFAgNPs whereas comparatively lower ABTS radical scvenging activity (84.59 %) was observed in NFAgNPs. The biogenic NFAgNPs also exhibited strong antibacterial activity against selected gram positive and negative human pathogenic bacteria. The highest percentage of growth inhibition in E. coli i.e (95.03 ± 1.16) followed by P. aeruginosa (94.24 ± 1.27) and K. pneumoniae (91.90 ± 2.69) after exposure to 1mg/mL concentration of NFAgNPs. Similarly, 63.03 ± 0.5 percentage growth inhibitory effects occurred in Gram positive S. aureus. Thus, the studied characteristics of biosynthesized NFAgNPs imply their relevance as a probable antioxidant and antibacterial agent. https://biomedpharmajournal.org/vol13no1/studies-on-antioxidant-and-antimicrobial-potential-of-biogenic-silver-nanoparticles-synthesized-using-nothapodytes-foetida-leaf-extract-wight-sleumer/ Notaphodytes Foetida Silver Nanoparticles Nfagnps Antioxidant Activity Antibacterial Activity