eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2020-03-28 13 1 27 31 10.13005/bpj/1856 30360 article Alexander Kiruthiga 1,2 Kesavaram Padmavathy 1 Praveen Shabana 3 Sumathi Gnanadesikan 4 Jeevan Malaiyan 4 Department of Microbiology, Research Laboratory for Oral and Systemic Health, Sree Balaji Dental College and Hospital, BIHER, Chennai. India. Department of Microbiology, Priyadarshini Dental College and Hospital, Pandur, Thiruvallur, India. Department of Laboratory Medicine, Meitra Hospital, Calicut, India. Department of Microbiology, Sri Muthukumaran Medical College Hospital and Research Institute, Chennai, India Pristinamycin(quinupristin/dalfopristin) is recommended for the treatment of serious infections caused by Enterococcus faecium. Nevertheless, screening for pristinamycin (Q/D) susceptibility is not routinely performed. Decreased in-vivo bactericidal activity of quinupristin/dalfopristin is reported in E. faecium with iMLS_B phenotype. Non-urinary clinical isolates of E.faecalis (n= 16) and E.faecium (n=9) were screened for inducible clindamycin resistance by D test and susceptibility to the standard antimicrobials by disc diffusion assay. High-level resistance to gentamicin and streptomycin (HLG^RHLS^R) was observed in 56% of the isolates. All the isolates were susceptible to vancomycin, linezolid and teicoplanin. Of the 25 isolates, 64%, 20%, 4% exhibited cMLS_B, iMLS_B phenotype and M type respectively. Three isolates (12%) belonged to an uncommon erythromycin-intermediately susceptible and clindamycin-resistant phenotype.  All the E. faecium isolates with iMLS_B phenotype were resistant to pristinamycin nevertheless, M type and Ery^ISclin^R phenotypes were found to be susceptible to pristinamycin. Routine screening for inducible clindamycin resistance among E.faecium, would detect iMLS_B/cMLS_B  phenotypes thereby, predict the possible decrease  in-vivo activity/clinical inefficacy of pristinamycin. https://biomedpharmajournal.org/vol13no1/higher-incidence-of-pristinamycin-resistance-among-enterococcus-faecium-with-imlsbcmlsb-phenotype/ Enterococcus Faecium HLGR HLSR Pristinamycin