eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2019-12-28 12 04 2099 2104 10.13005/bpj/1845 29747 article Ummul Khairi Amsyah 1 Mochammad Hatta 2 Hasanuddin Tahir 3 Gemini Alam 4 Asmawati Asmawati 3 Gradute School, Faculty of Medicine, University of Hasanuddin, Makassar, Indonesia Molecular Biology and Immunology Laboratory, Faculty of Medicine, University of Hasanuddin, Makassar, Indonesia Departement of Periodontology, Faculty of Dentistry, University of Hasanuddin, Makassar, Indonesia Phytochemical Laboratory, Faculty of Pharmacy, University of Hasanuddin, Makassar, Indonesia Periodontitis therapy originating from local natural resources is still lacking so it needs to be researched and developed, one of the natural resources from Soppeng Regency in South Sulawesi, Indonesia is the purple miana leaf. It has not been reported before about the effect of purple miana leaf extract on periodontitis related to IL-10 mRNA expression. This study aims to determine the effect of purple miana leaf extract on IL-10 mRNA expression in rat induced by Aggregatibacter actinomycetemcomitans. Rats were divided into three groups, purple miana leaf extract (PMLE), negative control (aquades), antibiotic (levofloxacin nine mg/kg body weight) as positive control. Rat blood was drawn before (H1) and after induction of 3x10⁸ cfu/ml Aggregatibacter actinomycetemcomitans in the gingival sulcus of the mandibular anterior teeth/after periodontitis (H8) and seven days after intervention (H15). IL 10 mRNA expression was measured by Real-time PCR. The results obtained are processed using SPSS. There were no significant differences in H1-H8 in all groups. IL-10 mRNA expression on H8-H15 has a different pattern between PMLE, negative control and positive control. In the negative control, there was a decrease in IL-10 mRNA expression in H8-H15. In PMLE and positive control, an increase in IL-10 mRNA expression. The treatment of purple miana leaf extract in rat induced with Aggregatibacter actinomycetemcomitans significantly had the same effect as levofloxacin on IL-10 mRNA expression. https://biomedpharmajournal.org/vol12no4/expression-of-il-10-in-a-actinomycetemcomitans-induced-rat-treated-by-purple-miana-leaves/ IL-10 miana leaves  A.actinomycetemcomitans  periodontitis Real-time PCR