eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2019-12-28 12 04 1801 1809 10.13005/bpj/1811 29753 article Bat-Erdene Jargalsaikhan 1 Narangerel Ganbaatar 1 Myadagbadam Urtnasan 2 Nyamdolgor Uranbileg 3 Dagvatseren Begzsuren 1 Department of Pharmacology, Institute of Traditional Medicine and Technology, Ulaanbaatar, 17032, Mongolia Department of Pharmacy, Institute of Traditional Medicine and Technology, Ulaanbaatar, 17032, Mongolia Department of Pathology, Institute of Veterinary Medicine, Ulaanbaatar, 17024, Mongolia Polyherbal formulation (PHF) is composed of Artemisia santolinifolia Turcz, Saussurea salicifolia L. and Hippophae rhamnoides L., which mainly used for inflammatory disorders in traditional Mongolian medicine. The aim of the study was to evaluate the anti-inflammatory effect of PHF in carrageenan and lipopolysaccharide (LPS) induced models of inflammation. The total active constituents of 20% ethanol extract of PHF was determined, using Folin-Ciocalteu reagent and aluminum chloride reagent, respectively. Inflammation models were induced by 1% carrageenan and LPS 7.5 mg/kg in the experimental groups. The levels of serum tumor necrosis factor- α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), and high mobility group box 1 protein (HMGB-1) were measured in PHF pretreatment groups by enzyme-linked immunosorbent assay (ELISA). The lungs were harvested and evaluated for histopathological assessment on 12 hours after LPS administration. The content of total phenolic was 28.5±0.12 mg/g and flavonoids 12.4±0.42 mg/g. After 60, 120, 180, 240 and 300 min, the data indicate that PHF 75, 150 and 300 mg/kg was significantly effective reducing paw edema volumes induced by carrageenan compared to control (p<0.01). PHF pretreatment significantly reduced levels of serum TNF-α, IL-1β and IL-6 at 300 minutes after carrageenan injection. Moreover, pretreated with PHF 150 mg/kg groups serum levels of TNF-α, IL-1β and HMGB-1 were significantly (p<0.01) reduced compared with the control group after LPS injection. It showed less inflammation and change of pulmonary structure compared with the LPS group at 12 hours after LPS injection. From the results of the study, it was demonstrated that PHF had sufficient potential to treat inflammatory disorders by reducing pro-inflammatory cytokines. https://biomedpharmajournal.org/vol12no4/anti-inflammatory-effect-of-polyherbal-formulation-phf-on-carrageenan-and-lipopolysaccharide-induced-acute-inflammation-in-rats/ Acute lung inflammation interleukin Mongolian herbal medicine paw edema pro-inflammatory cytokine