eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2019-09-25 12 3 1531 1541 10.13005/bpj/1784 28724 article G. V.Zodape 1 V. S. Gaikwad 1 Department of Zoology, S.S. and L.S. Patkar College of Arts and Science and V.P. Varde College of Commerce and Economics, S.V. Road, Goregaon (West), Mumbai- 400 062, India Fresh seeds of Piper nigrum were procured from the botanical garden of KokanKrushiVidyapeeth, Dapoli, Ratnagiri. The ethanolic extract of the seeds was carried out by soxhlate extraction method. Sixty four (64) Sprague- Dawley rats (average weight 150 - 240 g) of each sex were used for the experiment. The drugs ETH and PAS drug and Piper nigrum were given to respective groups daily for 28 days. At the end of study various biochemical parameters were analyzed from serum such as, ACP, ALP, SGOT (AST), SGPT (ALT), Bilirubin, Total Cholesterol, HDL Cholesterol, and TGL. Liver tissues were analyzed for Histopathology. The data was statistically analyzed by one way analysis of variance (ANOVA). The value p< 0.05 considered as significant. Administration ETH and PAS in Sprague-Dawley rats showed hepatotoxicity in test groups which was confirmed by biochemical parameters and histoarchitecture examination of liver. Ethanolic extract of Piper nigrum (Linn.) seeds were administered to the test groups have ameliorated the toxic effect of the drugs. It is concluded that the Piper nigrum (Linn.)  acts as hepato-protective agent hepato-toxicity caused by  Ethionamide and Para amino salicylic acid in Sprague-Dawley rats . https://biomedpharmajournal.org/vol12no3/effect-of-piper-nigrum-l-on-hepatotoxicity-induced-by-ethionamide-and-para-amino-salicylic-acid-in-sprague-dawley-rats/ Histoarchitecture Liver PAS Piper Nigrumeth Rats