eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2019-09-25 12 3 1167 1173 10.13005/bpj/1745 28367 article Muhammad Yulis Hamidy 1,2 Fadil Oenzil 3 Yanwirasti 4 Yufri Aldi 5 Biomedical Programme, Faculty of Medicine, Andalas University, Padang, Indonesia. Department of Pharmacology, Faculty of Medicine, Universitas Riau, Pekanbaru, Indonesia. Department of Biochemistry, Faculty of Medicine, Andalas University, Padang, Indonesia. Department of Anatomy, Faculty of Medicine, Andalas University, Padang, Indonesia. Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Andalas University, Padang, Indonesia. To evaluate the effect of andrographolide on monocyte chemoattractant protein-1 (MCP-1) expression at the initiation stage of atherosclerosis in rats induced by an atherogenic diet. The research was conducted on 27 rats divided into 3 groups (n=9). Group 1 was given a standard diet. Group 2 was given an atherogenic diet of vitamin D3 700.000 IU/kg on the first day followed by 5% goat fat, 2% cholesterol, 0.2% cholic acid and standard diet up to 100% for 2 days to induce atherosclerosis initiation stage. Group 3 was given an atherogenic diet and treated with andrographolide 40 mg/kg. An immunohistochemical examination was performed to determine the expression of MCP-1. Data analysis using one-way Anova followed by post hoc test. The results showed the expression of MCP-1 in group 1 was 6.61 + 1.90, in group 2 was 32.99 + 3.74 and in group 3 was 9.61 + 2.47. There was a significant difference between group 3 treated with andrographolide 40 mg/kg compared with group 2 (p<0.001). There was no significant difference between group 3 treated with andrographolide 40 mg/kg and group 1 (p>0.05). In conclusion, andrographolide may inhibit MCP-1 expression at the initiation stage of atherosclerosis in the andrographolide treated rats. Thus, andrographolide could be a potential anti-atherosclerosis drug. https://biomedpharmajournal.org/vol12no3/effect-of-andrographolide-on-monocyte-chemoattractant-protein-1-expression-at-the-initiation-stage-of-atherosclerosis-in-atherogenic-diet-fed-rats/ Andrographolide Atherosclerosis MCP-1 Atherosclerosis