eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2019-09-25 12 3 1587 1593 10.13005/bpj/1789 28734 article Numlil Khaira Rusdi 1,2 Jeanne Adiwinata Pawitan 3,4,5 Doctoral Program for Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia Faculty of Pharmacy and Science, University Muhammadiyah Prof. DR. Hamka, Jakarta, Indonesia Department of Histology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia Stem Cell Medical Technology Integrated Service Unit, Dr. Cipto Mangunkusumo General Hospital/Faculty of Medicine Universitas Indonesia, Indonesia Stem Cell and Tissue Engineering Research Center, Indonesia Medical Education and Research Institute (IMERI), Faculty of Medicine Universitas Indonesia, Indonesia Immunotherapy for cancer treatment continues to be developed and various strategies have been carried out including bioengineering. This endeavour requires development of technology, and efforts to find specific and sensitive tools to monitor immune responses during and after therapy. The purpose of this mini-review was to discuss cancer immunotherapy using T cell and immune checkpoint blockade therapy, as well as immunotherapy monitoring methods using flow cytometry (FCM). Bioengineering of T lymphocytes for immunotherapy and immune checkpoint blockades can be combined with nanoparticles as drug delivery carrier against cancer to increase drug distribution to tumor cells, as well as T cell stimulation regulation to reduce autoimmune effects. In addition, T cell engineering  can also prevent Host versus Graft alloreactivity in chimeric antigen receptor (CAR) T cell administration. FCM is a monitoring method that is widely used in pre-clinical and clinical cancer immunotherapy studies. https://biomedpharmajournal.org/vol12no3/cancer-immunotherapy-and-flow-cytometry-in-immunotherapy-monitoring/ Bioengineering Cancer Immunotherapy Immune Checkpoint Flow Cytometry T Cells