eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2019-06-25 12 2 533 538 10.13005/bpj/1672 27442 article Dalia Medhat 1 Zakaria El-Khayat 1 Mona El-Banna 1 Yasmin Abdel-Latif 1 Safaa Morsy 1 Sherien M. El-Daly 1 Medical Biochemistry Department, Medical Division, National Research Centre, Dokki, Giza, Egypt. Ethanol-induced diseases include oxidative mechanisms by which prolonged endoplasmic reticulum (ER) stress results in genesis and accumulation of cytotoxic total fatty acid ethyl esters (FAEEs, non-oxidative metabolites of ethanol). FAEEs participate in the pathogenesis of alcoholic lung disease. Polyunsaturated fatty acids (PUFA) offer a possible protective effect against damage induced by ethanol inhalation. The present study aimed to investigate the protective effect of flaxseed and fish oils administration against toxicity induced by ethanol inhalation. Forty healthy female albino rats were divided into four groups (control, ethanol, flaxseed and fish oils). Lung superoxide dismutase (SOD) and malondialdehyde (MDA) were measured. Plasma advanced oxidation end product (AOPP) and phosphatidylinositol 3- kinase (PI₃K) were determined. Erythrocyte membrane fatty acids were extracted and fractionated by HPLC. Ethanol inhalation results in significant increase in lung MDA, plasma AOPP and erythrocyte membrane arachidonic acid (AA), linolenic acid (LA), and oleic acid (OA) along with a significant decrease in erythrocyte membrane alpha-linolenic acid (ALA), lung SOD, and plasma PI₃K while pretreatment with flaxseed and fish oils daily (1.2 ml/kg) significantly attenuated these parameters. Supplementation of marine PUFAs reduced the oxidative stress induced by ethanol inhalation in experimental animals. https://biomedpharmajournal.org/vol12no2/protective-effect-of-polyunsaturated-fatty-acids-against-experimental-lung-injury-induced-by-acute-ethanol-inhalation/ Advanced Oxidation Protein Product Arachidonic Acid Cell Membrane HPLC Phosphatidylinositol 3-Kinase