eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2019-06-25 12 2 961 963 10.13005/bpj/1722 27554 article Ana Khusnul Faizah 1 Yanu Andhiarto 2 Pramudita Riwanti 3 Department of Clinical Pharmacy, Hang Tuah University, Surabaya, Indonesia. Department of Biology Pharmacy, Hang Tuah University, Surabaya, Indonesia. Department of Chemical Pharmacy, Hang Tuah University, Surabaya, Indonesia. Fish oil which contains omega 3 with the most types of Eicosapentaenoic Acid (EPA) and Docosahexaenoic Acid (DHA) has the effect of pharmacology as anti-inflammatory, antioxidant and is good for heart disease where probably it can be used  beneficially as an analgesic. Analgesics have several side effects including gastric ulcer, bleeding, even Steven Johson’s Syndrome, have been attributed to their use. This research aims to determine the analgesic effect of fish oil on male white mice induced to produced peripheral pain. We used acetic acid-induced male BALB/c white mice to investigate analgesic effect fish oil in vivo by Writhing method.  From this research, fish oil decreased writhings number in mice dose 5, 10, 20 and 30 mg/kg compared to negative control (P<0.001). The percentage inhibition of writhing at the dose 30 mg/kg is  the hignest among other dose (42.64%), while in the group positive control (acetylsalysilic acid 100 mg/kg) is 68.26%. We concluded that fish oil has analgesic effect which reduced writhings in male mice induced by acetic acid to produce peripheral pain. https://biomedpharmajournal.org/vol12no2/in-vivo-analgesic-activity-of-omega-3-on-mice-induced-peripheral-pain/ Analgesic Antiinflammatory Fish Oil Marine Nutraceutical Omega-3 Pharmaceutical