eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2019-06-25 12 2 511 525 10.13005/bpj/1670 27337 article Badr E. El-Bialy 1 Neveen G. El-Boraey 2 Ragaa A. Hamouda 3 Mohamed M. Abdel-Daim 4 Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, 32897, Egypt. Department of Microbial Biotechnology, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Sadat City, 32897, Egypt. Department of biology, Faculty of sciences and Arts Khulais, University of Jeddah, Saudi Arabia Department of Microbial Biotechnology, Genetic Engineering and Research Institute, Sadat University, Sadat city, 32897, Egypt. Department of Pharmacology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt. Carbon tetrachloride (CCl₄) is used extensively as an industrial solvent and considered the best-characterized experimental animal model of xenobiotic-induced hepatic toxicity via reactive oxygen species (ROS) generation. This study was designed to evaluate the protective effects of Spirulina platensis (SP) versus Spirulina platensis supplemented with thiamine (SPt) against subacute CCl₄ toxicity in rats. Rats were divided into six equal groups; Control vehicle (0.5 ml/rat 1:1 olive oil in water), SP (800 mg/kg b.wt.), SPt (800 mg/kg b.wt.), CCl₄ (1ml/kg b.wt.), SP + CCl₄ and SPt + CCl₄. All treatments were orally and daily for a month except CCl₄ was given three times weekly. CCl₄ caused significant reduction in body weight gain, haemoglobin content and haematocrit percentage accompanied by leukocytosis, granulocytosis, monocytosis and lymphocytopenia. Moreover, there were significant increase in the levels of serum ALT, AST; total, direct and indirect bilirubin; urea and creatinine of CCL₄- intoxicated rats. CCL₄- induced significant increase of malondialdehyde levels with significant reduction of catalase activity in liver and kidney. In addition, hepatic and renal various histopathological alterations were recorded. SP and SPt ameliorated almost these changes while they couldn’t reverse the reduction of body weight gains and red blood indices. The more potent effects on measured parameters were elucidated by SPt. In conclusion SP and SPt could be used as natural antioxidant supplements to counteract the CCl₄ adverse effects. https://biomedpharmajournal.org/vol12no2/comparative-protective-effects-of-spirulina-and-spirulina-supplemented-with-thiamine-against-subacute-carbon-tetrachloride-toxicity-in-rats/ Antioxidants Biomarkers CCl₄ Haematology Oxidative Stress Spirulina