eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2019-06-25 12 2 857 862 10.13005/bpj/1710 27571 article Nurhidayah Ab-Rahim 1 Wan Ismahanisa Ismail 1 Muhammad Nabil Fikri Rosdan 1 Mohd Hafiz Mail 2 Roz Azinur Che Lamin 3 Salmah Ismail 4 Fakulti Sains Kesihatan, Universiti Teknologi MARA, Cawangan Pulau Pinang (Kampus Bertam), Malaysia. Malaysian Institute Pharmaceuticals and Nutraceuticals (IPHARM), NIBM, Pulau Pinang, Malaysia. Fakulti Farmasi, Universiti Teknologi MARA, Cawangan Pulau Pinang (Kampus Bertam), Malaysia. Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur. The rapid emergence of resistance bacteria toward various antibiotics may associate with higher medical cost and increase mortality rate. Hoya diversifolia was used to cure skin diseases and alleviate rheumatism pain. The aim of this study is to evaluate in vitro antibacterial properties of H. diversifolia ethanolic leaves extract against several Gram positive and Gram negative bacteria. The antibacterial study was determined based on pattern of inhibition zones using disc diffusion assay and also minimal inhibitory concentration (MIC). It is shown that the extract can inhibit the growth of methicillin-resistant Staphylococcus aureus, Escherichia coli and Bacillus cereus. The lowest MIC values of extract were 25 mg/mL for MRSA and E. coli as well as 100 mg/mL for B. cereus at 24 and 48 hours of incubation period. The plant had potential to act as antibacterial agent that can be applied in pharmaceutical and cosmetic fields. https://biomedpharmajournal.org/vol12no2/antibacterial-activity-of-hoya-diversifolia-ethanolic-leaves-extract/ Antibacterial Disc Diffusion Hoya Diversifolia Minimum Inhibitory Concentration