eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2019-03-25 12 1 57 63 10.13005/bpj/1613 26716 article Anuj Kumar 2 Akhilesh Kumar Rana 2 Amit Singh 2 Alok Singh 1 Department of Pharmacology, All India Institute of Medical Sciences Raipur Chhattisgarh, India. Institute of Medical Sciences, Banaras Hindu University, Varanasi Uttar Pradesh India. To study the effect of methanolic extract of Phyllanthus niruri on animal model of Diabetes Mellitus. Diabetes Mellitus was induced in rats by injecting Streptozotocin (60mg/kg) intraperitonealy. Blood glucose was measured on day 3 by GOD-POD method. Rats having fasting blood glucose >250 mg/dl were further selected for study. Four groups were created i.e. Control, Control+Streptozotocin, Streptozotocin+ Metformin(75mg/kg) and Streptozotocin+ extract of P. niruri (250mg/kg). Each group was consisting of 6 rats of either sex. Metformin and experimentalextract were administered for 21 days. Blood Glucose was measured on day 7 and 21. Triglyceride, Cholesterol and Leptin level were also measure by commercially available kit. Anti-oxidant potential was assessed by estimating extent of Lipid peroxidation (LPO) by Malondialdehyde (MDA), Nitric oxide (NO), Superoxide dismutase (SOD) and Glutathione (GSH) in four different tissues i.e. Liver, Kidney, Pancreas, Muscle on day 21. Unpaired and paired student’s t-test were applied for statistical analysis using SPSS Software. The extract of P. niruri showed significant decrease in blood glucose level on day 21 (p-0.04). The treatment didn’t show significant lowering of Leptin and Cholesterol level however Triglyceride level was significantly reduced (p-0.05). The treatment group showed improvement in oxidative stress by increasing SOD and GSH and decreasing LPO and NO activity. The study showed anti-hyperglycemic and anti-oxidative properties of methanolic extract of P.niruri. https://biomedpharmajournal.org/vol12no1/effect-of-methanolic-extract-of-phyllanthus-niruri-on-leptin-level-in-animal-model-of-diabetes-mellitus/ Anti-hyperglycemic Anti-oxidant  Phyllanthus niruri