eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2018-12-25 11 4 1763 1771 10.13005/bpj/1547 23959 article Walid Abu Rayyan 1 Sami A. G. Alshammari 2 Abdulaziz M. F. ALSammary 2 Mutab S. S. AL-Shammari 2 Nisreen Seder 4 Luay F. Abu-Qatouseh 1 Department of Pharmacology and Biomedical Sciences, Faculty of Pharmacy and Medical Sciences, University of Petra, Amman, Jordan. College of Medicine, University of Hail, Hail, KSA. Department of Pharmaceutical Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy and Medical Sciences, University of Petra, Amman, Jordan. School of Biomedical Science, Faculty of Health Sciences, University Sultan Zainal Abidin, 21300 Kuala Nerus, Terengganu, Malaysia. Department of Basic Sciences, College of Science and Health Professions, King Saud Bin Abdulaziz University for Health Sciences, Jeddah, Saudi Arabia. Department of Medical Laboratory Sciences, Faculty of Science, Mutah University. Mutah, Jordan. The increment in numbers of antimicrobial resistant strains along with the scarcity of new targets for drug industry has forced scientists to investigate deeply in the natural resources for new compounds with antimicrobial activity. Pergularia tomentosa is a member of the Apocynaceae family found in a wide geographical region including the Gulf region, Africa, and the Middle East. It is used as a remedy for the treatment of skin sores, asthma, and bronchitis. Dried plants of Pergularia tomentosa were subjected to extraction by using a Soxhlet extractor process to obtain essential oil and characterized by HPLC- Mass Spectroscopy (GC-MS). The essential oil was evaluated for antibacterial activity against pathogenic microorganisms by well diffusion method and confirmed by microdilution method. Additionally, we measured the antioxidant activity of the extracts using DPPH reagent. Phytochemical analysis has revealed variation in compositions and concentrations of P. tomentosa constituents grown in Hail from other agricultural regions. The lowest MIC was recorded with ethyl acetate extract MIC of 6.25 mg/ml against S. typhi, whereas, the ethanolic extract had the broadest effective against the five strains with a MIC of 25 mg/ml. In conclusion, we summarize a variation between the phytochemical constituents of P. tomentosa plants grown in the district of Hail and other geographical regions. In addition, there are several natural phytocompounds with an antimicrobial activity could be a good target for the antimicrobial and antioxidants industry. https://biomedpharmajournal.org/vol11no4/the-phytochemical-analysis-and-antimicrobial-activity-of-pergularia-tomentosa-in-north-east-kingdome-of-saudi-arabia-ksa/ Antimicrobial Antioxidants HPLC-MS Pergularia Tomentosa Phytochemical