eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2018-12-25 11 4 2043 2050 10.13005/bpj/1581 24274 article G. Nagarajaperumal 1 V. E. Idachristi 1 M. Karpakavalli 1 S. Mohan 1 Karpagam College of Pharmacy, Coimbatore, Tamil Nadu, India. Diabetes mellitus (DM) is most significant health problem in various developed and developing countries due to alteration of various clinical and pathological factors. Current work was intended for examine in vitro and in vivo anti diabetic competence of Cassia Auriculata flowers and its phytochemical analysis. The inhibitory effects on carbohydrate digestive enzyme α- amylase interaction with various extracts of Cassia Auriculata were contrast with acarbose. Hypoglycemic activity was executed with standard as a glibenclamide. Study indicated ethanolic extract showed higher action on α- amylase inhibition with assessment of IC₅₀ value; 43.6%. Based on the above results of in vitro studies were used to selected ethanolic extract of Cassia Auriculata flower used for further study. Results of animal studies indicated that the ethanolic extract of C. Auriculata has shown dose dependent action (200 mg/ kg (1.20±0.91↓) and 400 mg / kg (4 ± 0.01↓) when compared to control and standard drug treated groups. Our study confirmed to ethanolic extract work through the α- amylase inhibition mechanism. Our view bioactive constituents confirm anti diabetic capacity and afford methodical source for validation of Cassia Auriculata flowers in ayurvedic formulations on diminution of DM prevalence. https://biomedpharmajournal.org/vol11no4/in-vitro-and-in-vivo-evaluation-of-potential-anti-diabetic-efficacy-on-cassia-auriculata-flowers/ Acarbose Alfa Amylase and Hypoglycemic Cassia Auriculata Glibenclamide