eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2018-12-25 11 4 1755 1761 10.13005/bpj/1546 24319 article Eun-Jin Yang 1 Sungchan Jang 2 Kwang Hee Hyun 3 Eun-Young Jung 4 Seung-Young Kim 2 Chang-Gu Hyun 1 Department of Chemistry and Cosmetics, Jeju National University, Jeju 63243, Korea. Department of Pharmaceutical Engineering and Biotechnology, Sunmoon University, Chungnam 31460, Korea. Helios Co., Ltd., Sanchundan Dong-gil 16, Jeju 63243, Korea. Department of Beauty Art, Chejuhalla University, 38 Halladaehak-ro, Jeju 63092, Korea. The anti-inflammatory activity and non-toxicity of Sonchus oleraceus extract (J6) were tested by measuring its effect on the levels of nitric oxide (NO), prostaglandin E2 (PGE₂), and the pro-inflammatory cytokines, interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α), in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. We treated the RAW264.7 cells with various concentrations (50, 100, or 200 μg/mL) of J6. Our results showed that J6 inhibited the production of NO, PGE₂, and pro-inflammatory cytokines in a concentration-dependent manner, without compromising cell viability. In addition, we provided supporting evidence that the inhibitory activity of J6 on the production of NO and PGE₂ occurred via the downregulation of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), respectively. Our findings suggest that J6 is a new source for anti-inflammatory drugs and ingredients for healthcare products that include functional cosmetics. https://biomedpharmajournal.org/vol11no4/anti-inflammatory-activity-of-sonchus-oleraceus-extract-in-lipopolysaccharide-stimulated-raw264-7-cells/ Anti-inflammation COX-2 iNOS PGE2 Sonchus oleraceus TNF-α