eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2018-09-21 11 3 1239 1245 10.13005/bpj/1485 22403 article Bahaa Al-Trad 1 Mahmoud A. Al-Qudah 2 Mazhar Al-Zoubi 3 AlaaAl-Masri 1 Riyadh Muhaidat 1 Janti Qar 1 Department of Biological Sciences, Yarmouk University, Irbid, Jordan. Department of Chemistry, Yarmouk University, Irbid, Jordan. Department of Basic Medical Sciences, Faculty of Medicine, Yarmouk University, Irbid, Jordan. Department of Food Science and Nutrition, Faculty of Agriculture, Jerash University, Jerash, Jordan. Previous studies indicated that the extracts from different Ephedra species have antibacterial, antifungal and antioxidant activities. However, none of the published report described the phytochemical components and the antioxidant capacities of Ephedra alte belonging to the family Ephedraceae. To evaluate the in-vitro and in-vivo antioxidant activities of the butanolic extract from stems of Ephedra alte from northern Jordan. Graded concentrations of butanolic extracts from stems of E. alte plant were subjected to four different in-vitro antioxidant assays (DPPH, ABTS, ferrous ion chelating and hydroxyl radical scavenging activities). The in-vivo effects of two different doses of the extract (200 mg/kg and 500 mg/kg, orally for 12 days) on the activities of serum and liver superoxide dismutase (SOD) and catalase (CAT) were measured in mice. Strong in-vitro antioxidant activities in a concentration-dependent manner were recorded. As well, significant increases in both liver and serum CAT enzyme activity and in serum SOD activity were observed in mice treated for 12 days with the extract. These results suggested that the butanolic extract from stems of exhibited significant in-vitro and in-vivo antioxidant activities, supporting the use of E. alte as an important source of natural antioxidants. https://biomedpharmajournal.org/vol11no3/in-vitro-and-in-vivo-antioxidant-activity-of-the-butanolic-extract-from-the-stem-of-ephedra-alte/ Antioxidant Butanol Extract Ephedra alte In vitro In vivo