eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2018-06-25 11 2 703 709 10.13005/bpj/1423 20361 article Vishnu T. S. 1 Palaniswamy M. 1 Department of Microbiology, Karpagam Academy of Higher Education, Coimbatore 641021, Tamil Nadu, India. Violacein was isolated from Chromobacterium vaccinii CV5, a Gram negative bacterium collected from the Well water near a paddy field- Nedumangadu, Kerala, India. In the current antioxidant study, the activities of violacein were analyzed by different scavenging assay. In vitro antioxidant activity of violacein pigment was studied with the antioxidant, hydrogen peroxide DPPH, nitric oxide, hydroxyl and superoxide. The results suggest that the violacein possess potent activity of antioxidant against DPPH, superoxide, nitricoxide, hydrogen peroxide and hydroxyl with IC₅₀ values of 297.88 µg/mL, 312.89 µg/mL, 410.17 µg/mL, 296.74 µg/mL and 292.74 µg/mL respectively. The activity was comparable with the standard L-ascorbic acid. Natural compounds are very much used for the treatment of chronic diseases because of their effectiveness and have less harmful as compared to synthetic and artificial drugs. The present study’s shows the ability of the pigments as a source of natural antioxidants which has various applications in pharmaceutical industries. https://biomedpharmajournal.org/vol11no2/systematic-approach-on-evaluating-the-in-vitro-antioxidant-activity-of-violacein-novel-isolate-chromobacterium-vaccinii-cv5/ Antioxidant Chromobacterium DPPH Pigment Violacein