eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2018-06-25 11 2 1023 1030 10.13005/bpj/1461 21053 article Jayalalitha Sathiyamoorthy 1 Vidyarani Shyam Sundar 2 N. Aravindha Babu 2 Subbaih Shanmugham 3 Jagadeesan G. Mani 3 Ponnuraja Chinnaiyan 4 Department of Biotechnology, Dr. M.G.R Educational and Research Institute Maduravoyal, Chennai-95, India. Centre of Oral Cancer Prevention and Research, Sree Balaji Dental College and Hospital, Bharath Institute of Higher Education (Bharath University), Pallikaranai, Chennai-99, India. Centre of Surgical Oncology Government Royapettah hospital and Kilpauk Medical College, Chennai, India. Department of Statistics, National Institute of Research in Tuberculosis, No: 1, Sathiyamoorthy Road, Chetpet, 600031,Chennai, India. The present investigation was performed in South Indian Populations to determine the hotspot mutation frequency in Oral Squamous Cell Carcinoma (OSCC) patients with PIK3CA gene Exon 9 and Exon 20 and its correlations with help of their clinical characteristics leading to these mutations. PI3KCA belongs to a group of regulatory heterodimeric lipid kinase which is involved in proliferation of cells, apoptosis and as well in metastasis which is controlled by PIK3CA gene is subjected to high frequency of somatic mutation in various tumors including OSCC. Total of 25 OSCC patients samples comprising of male and female subjects from Government tertiary care Centre were included in this study. Tumor specimen samples were collected and amplified for PIK3CA gene by PCR and subjected to genomic DNA Sequencing. Our findings showed total of 20% of oncogenic frequency in PIK3CA gene. We also observed two hot spot mutations (E545K) in exon 9 gene and three hot spot mutations (H1047Q, H1047Y, H1048Q) in exon 20 gene in our study populations. 0 Based on our findings it may be concluded that PIK3CA gene Exon 9 and Exon 20 contributes to a major role in pathogenesis on OSCC among South Indian populations may act as therapeutic target for a anticancer drug for the treatment OSCC. https://biomedpharmajournal.org/vol11no2/study-on-pik3ca-gene-mutations-in-oral-squamous-cell-carcinoma-among-south-indian-populations/ hotspot   OSCC PIK3CA tumor