eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2017-12-21 10 4 1665 1675 10.13005/bpj/1278 17172 article Mohammed Hamad 1 Wael Abu Dayyih 2 Alsabbagh Rafal 2 Alice Abu Dayyih 3 Israa Al Ani 4 Eyad Mallah 2 College of Science and Health Professions, King Saud Bin Abdulaziz University for Health Sciences, Jeddah, Saudi Arabia. Faculty of Pharmacy and medical Sciences, University of Petra, Amman, Jordan. Faculty of Chemistry, Biology, and Pharmacy, Hochschule Fresenius, Germany. Faculty of Pharmacy and Medical Sciences, Al-Ahliyya Amman University, Amman, Jordan. Faculty of Pharmacy, the University of Jordan, Amman, Jordan. Food-drug interactions may affect pharmacokinetics or pharmacodynamics of a drug. Failure to identify and properly manage drug-nutrient interactions may lead to serious consequences. In this study, we try to develop and validate a simple and sensitive analytical method for measuring of glimepiride in rat plasma and examine of any possible interactions of glimepiride with different fruit juices. A mixture of 80 % methanol and 20 % of 0.1% FA in water was used as a mobile phase and ACE 5 C18 (50 X 2.1 mm, 5μm) column were employed; and clarithromycin was used as internal standard. This method was confirmed in terms of linearity, precision, accuracy, stability and system suitability. The validated method was used to examine the effects of grapefruit and licorice of glimepiride pharmacokinetic in rat plasma. The kinetic data shows that glimepiride plasma level was high when combined with licorice, and at the same level when combined with grapefruit. The administration of grapefruit juice concomitantly with glimepiride shows insignificant effect on its C_max, elimination half-life and clearance (P>0.05). While for licorice juice, it has no significant effects on glimepiride pharmacokinetic parameters. A simple and sensitive analytical method for determination of glimepiride in rat plasma has been developed and validated by using HPLC-MS. Also, we found that fruit juices can alter glimepirides pharmacokinetic if they were taken simultaneously. https://biomedpharmajournal.org/vol10no4/the-effect-of-some-fruit-juices-on-glimepiride-pharmacokinetic-in-rat-plasma-by-using-high-performance-liquid-chromatography-mass-spectrometry/ glimepiride  grapefruit  licorice HPLC-MS pharmacokinetics