Iztleuov M, Zhexenova A, Abdildayeva S, Iztleuova G, Zhiengalieva A, Altayeva A, Taskozhina G, Jumagalieva J, Umirzakova Z, Shurenova M, Sisembayeva A. Influence of Boron Compounds on Chromium-Induced Hemorheology Disorders in Rats. Biomed Pharmacol J 2017;10(4).

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Manuscript received on :March 17, 2017
Manuscript accepted on :March 30, 2017
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Marat Iztleuov, Azhar Zhexenova, Saule Abdildayeva, Gulmira Iztleuova, Aliya Zhiengalieva, Akmaral Altayeva, Gulaym Taskozhina, Jamila Jumagalieva, Zhanat Umirzakova, Mahabbat Shurenova and Akmoldir Sisembayeva

West Kazakhstan Marat Ospanov State Medical University, Aktobe, Kazakhstan.

Corresponding Author E-mail: zhanat_us@mail.ru

DOI : https://dx.doi.org/10.13005/bpj/1292

Abstract

The authors of this study evaluated the protective influence of boric acid on chromium-induced blood rheology disorders. The experiment was carried out on Wistar rats, which were divided into three groups: Group 1 - the control group; Group 2 - rats with simulated chromium-induced hemorheology disorders; Group 3 - rats, in which boric acid was administered throughout 10 days on the background of chromium-induced hemorheology disorders. In rats with simulated chromium-induced hemorheology disorders, one could observe decrease in deformability of red blood cells and in hematocrit level on the background of increased aggregation, peroxide hemolysis and osmotic fragility of red blood cells. In the third group, corrective application of boric acid inhibited development of blood rheology disorders, i.e., its protective action was revealed.

Keywords

Boric Acid; Hemorheology;   Potassium Dichromate; Protective Action Rat;

Introductıon

Quantitative and qualitative changes in blood rheology occur under the impact of industrial chemicals and these changes reflect their chemical profile (Krivokhizhina, et al., 2006). Since red blood cells make 93% of formed elements, changes in their physico – chemica properties hinder implementation of their main function – transport of oxygen in microvasculature, which leads to the development of tissue hypoxia (lack of oxygen transfer or termination of its delivery, decrease in redox activity, development of structural and qualitative changes in cell membranes (Khetsuriani and Kipiani, 2002; Lukyanova, 2003)), increased risk of cardio – vascular diseases and relevant complications (Lowe, et al., 1997) as well as ischemic cerebrovascular diseases (Szapary, et al., 2004). Rheological properties of blood depend on many factors (Ormotsadze and Nadareishvili, 2002); they may change under the impact of various stress factors (Gyawali, et al., 2015) and chemicals (Zairova et al., 2006; Kotelnikov and Kotelnikova, 2005; Zimetti et al., 2006; Pagano and Faggio, 2015).

Chromium is a self-existing microelement. Its valence Cr⁺³ or Cr⁺⁶  influences absorption. The oxidation degree and solubility of chromium compounds determine their toxicity. The impact of hexavalent chromium Cr⁺⁶ has a number of negative effects, including neurotoxicity, hepatotoxicity, cardiotoxicity, renal toxicity, genotoxicity, carcinogenicity, immunotoxicity as well the development of microcytic hypochromic anemia (Kawanish et al., 2002; O’Brien et al., 2003; Bazarbekova, 2002; Sudha et al., 2011; Stout et al., 2009). Once inside the cell,  Cr⁺⁶  is restored to Cr⁺³; this is accompanied by the generation of reactive oxygen intermediates, which cause oxidation of macromolecules such as DNA and lipids (Aruldhas et al., 2005; Wise et al., 2008; Wang et al., 2011; Iztleuov, 2003; Iztleuov et al., 2011) and induce tissue damages in a number of organs, such as liver, pancreas, kidneys and the blood-vascular system (Stout et al., 2009; Solis-Heredia et al., 2000; Bagchi et al., 2002; Fatima et al.,  2005). Different people are exposed to high concentrations of  Cr⁺⁶  professionally, ecologically or internally (Mamyrbayev, 2012).

Boron is a conditionally self-existing element. Naturally, it exists in the form of borates. Physiological concentrations of boron compounds affect a wide range of metabolic processes (Hunt, 1998), which is “apparently” associated with their antioxidant effects (Turkez et al., 2007; Hu et al., 2014). Boron compounds have anti-inflammatory, antitumor and hypolipidemic properties (Barronco et al., 2008). Besides, these compounds are not genotoxic (Ornat and Konur, 2004; Oto et al., 2015).

The damaging impact of Cr⁺⁶ – induced oxidative stress is caused mainly by a hydroxyl radical, which damages macromolecules, forms protein crosslinks promoting protein denaturation and aggregation. Besides, it causes formation of secondary radicals by reacting with low-molecular compounds (Iztleuov, 2004). Oxidative stress develops when the content of antioxidants is reduced (Tapiero et al., 2004). Antioxidants can protect cells from free radicals in the presence of metal-induced oxidative stress (Valko et al., 2005). Once inside the body, boric acid (boron compounds) enhances the prooxidant – antioxidant balance (Bolanos et al., 2004; Turkez, 2008) and increases activity of antioxidant enzymes, thereby neutralizing reactive oxygen intermediates, eliminating and preventing oxidative damage of cell membranes in macromolecules. However, protective action of boron compounds in the presence of chromium-induced hemorheology disorders.

The aim of this study was to evaluate the protective influence of boric acid on chromium-induced blood rheology disorders.

Materials and Method

Experiments were performed on 24 male Wistar rats weighing 190 – 220 g. The animals were kept in plastic cages in observance of a certain light regime (12-hour light / 12-hour dark periods) at a temperature of 23 – 25⁰C, with free access to food and water. Experiments were conducted in the morning hours (9 AM – 12 PM). All manipulations were carried out in accordance with the European Convention for the Protection of Vertebrate Animals used for Experimental and Other Scientific Purposes (Strasburg: Council of Europe, 1986). The program of this experiment was discussed and approved by the regional ethics committee of the West Kazakhstan Marat Ospanov State Medical University.

Ten days after acclimatization, the animals were randomly divided into three groups (eight rats in each group): the first group comprised intact rats (control), the second and the third group comprised animals with simulated chromium-induced hemorheology (diselementosis) caused by a single intraperitoneal injection of potassium dichromate K₂Cr₂O₇,  purchased from LLP “Chemistry and Technology” (Kazakhstan) at the rate of 14 mg / kg of body weight (0,5LD₅₀). In contrast to the second group, boric acid H₃BO₃ purchased from OJSC “Farmak” (Ukraine) was administered orally after K₂Cr₂O₇  in rats from the third group – 5.0 mg / kg of body weight during 10 days. Selection of doses, routes of administration and duration were substantiated in the previous studies (Iztleuov et al., 2011) and chosen according to relevant literature data (Moore, 1997; Pahl et al., 2005).

Experimental animals were under ether anesthesia. Blood was collected from the heart using siliconed needles. Rheological properties of red blood cells was assessed by erythrocyte deformability index (EDI), erythrocyte aggregation coefficient (EAC), by erythrocyte peroxide hemolysis (EPH), hematocrit (Ht) as well as by erythrocyte osmotic fragility (EOF).

Erythrocyte deformability index was determined using the method developed by Zakharova N.B., Tselik N.I., and Klyachkin M.L. (1989). This method implies preparing the 60% erythrocyte suspension and 0.02 µl (20 µl) of the suspension is applied onto a filter paper with pore diameter of 4±1  µl. After 60 seconds, the stain diameter (D₁) is measured. Next, after 60 seconds, 20 uL (0.02 µl) of a 60% suspension is applied again to the center of the obtained spot and the spot diameter (D₂) is measured again. Erythrocyte deformability index is calculated using the following formula:

EDI=D₁/D₂ , where D₁ and D₂ – diameters of stains.

Erythrocyte aggregation coefficient (EAC) was determined using the method developed by V.A. Lapotnikov and L.M. Kharash (1982).

This method implies the following: erythrocyte aggregates in a blood sample are fixed by formalin. The difference in weight of erythrocyte aggregates fixed by formalin and single cells causes changes in erythrocyte sedimentation rate (ESR) as compared to the control sample without formalin. In order to determine erythrocyte aggregation, the authors used 0.1M phosphate buffer with pH 7.4, 0.077 M EDTA (ethylenediaminetetraacetate) 4% clarified formalin. These reagents were used to prepare working solutions: solution No.1 – 3 ml of 0.077 M EDTA, 5.0 µl of 4% clarified formalin, 12.0 µl of 0.1M phosphate buffer, pH – 7,4. Solution No.2 – 3,0 µl of 0.077 M EDTA, 17 µl of 0.1 M phosphate buffer, pH – 7,4. Sequence of procedures: using siliconed needles, blood by 0.5 µl  (500 µl) is poured in two centrifuge tubes containing 2 µl of solution No. 1 and No. 2, respectively. After blood was mixed with both solutions, ESR was determined in each sample using the incubator at 37⁰C.

Determination of peroxide hemolysis of erythrocyte membranes was performed according to the method developed by A.A. Pokrovskiy and A.A. Abrarov (1964), which is a modified macromethod developed by Gyorgy P., Cogan G., Rose C.S (1952).

Modification implied spectrophotometric (l = 543 nm) determination of erythrocyte content, hemolyzed in standard conditions under the impact of hydrogen peroxide (H₂O₂). The use of ultra-microanalysis was a specific feature of this modified method, which significantly reduced the volume of blood (20 µl, 0,02 µl) required for this experiment.

The level of hematocrit was measured by using the automated hematological analyzer CELL- DYN Ruby (Abbott, USA).

Erythrocyte osmotic fragility was determined by the Daisy method (Todorov, 1968).

Sequence of procedures: basic solution (NaCl -. 180,0 g, Na₂HPO₄ -27,31 g, NaH₂PO₄ ∙ 2H₂O – 4,86 g, Aquae dest -. 2000,0 ml (2,0 l), pH -7,4 ) was used to prepare dilutions corresponding to 0.85; 0.55; 0.5; 0.45; 0.4; 0,35 and 0,3% of NaCl. 5.0 µl of each dilution was put in a number of centrifuge tubes, and then 50 µl (0.05 µl) of blood was added, mixed up and left for at least 30 minutes at room temperature. Afterwards, these dilutions were centrifuged during 5 min at 2000 rpm (700 g). Optical density of the supernatant was measured at a wavelength of 543 nm by using a “Genesys -5” spectrophotometer (USA).

Cell hemolysis was calculated percentagewise as related to 100% hemolysis induced by 0,1% NaCl solution.

Table 1: The impact of boric acid on some chromium-induced blood rheology disorders in rats

Note: some parameters refer to both Table 1 and Table 2 – Group 1 – intact; Group 2 – rats with simulated chromium-induced hemorheology disorders; Group 3 – rats, in which boric acid was administered throughout 10 days on the background of chromium-induced hemorheology disorders; EDI – erythrocyte deformability index; EAC – erythrocyte aggregation coefficient, EPH – erythrocyte peroxide hemolysis; significant differences (р<0,05): asterisk – in relation to the control group; bold – in relation to data obtained from Group 2.

Table 2: Protective impact of boric acid on erythrocyte osmotic fragility

Results and Discussion

Analysis of the obtained data shows blood rheology disorders in the presence of chromium-induced diselementosis. This is displayed by significant reduction of erythrocyte deformability index and hematocrit level against the backdrop of increase in erythrocyte aggregation coefficient, erythrocyte peroxide hemolysis (Table 1) and in erythrocyte osmotic fragility (EOF). EOF is significantly increased (p <0,05) in the presence of 0.4; 0.45; 0.5; 0,55 and 0,85% NaCl (Table 2). Intake of boric acid on the background of chromium-induced hemorheology disorders leads to the improvement of blood rheological properties (p≤ 0,05).

Chromium causes a wide range of toxicological effects and biochemical dysfunctions that imply serious health risks (Mamyrbayev, 2012; Bielicka et al., 2005). Some studies show that Cr⁺⁶  and its compounds do not directly generate free radicals; however, reduction of Cr⁺⁶  to Cr+3, as well as the effect of Haber – Weiss and Fenton mechanisms (Lloid et al., 1998), imply the emergence of different radicals that cause damages characteristic of oxidative stress (Pritchard et al., 2000; Barrera et al., 2003). Consequently, one of the possible basic approaches used for prevention (correction) of K₂Cr₂O₇   – induced damage implies using agents (elements) with powerful antioxidant properties. Recent studies have shown that boron and its compounds displayed significant protective effects against damages induced by metals, such as aluminum and arsenic (Turkez et al., 2011; Kucukkurt et al., 2015). Erythrocytes are sensitive to the impact of heavy metals, including chromium compounds (Ryspekova et al., 2013); they present a convenient model to assess cytotoxicity of chemicals. Hemolysis and its value may serve as a stability test for cell membranes of erythrocytes (Pagano and Faggio, 2015). The latter are characterized by certain rheological properties (deformation and flow), due to which they have a lenticular shape, high flexibility, elasticity and deformability. Deterioration of their rheological properties trigger the occurrence of hemorheology disorders in the presence of various diseases (Muraviov and Tikhomirova, 2009; Sharapova, 2012; Baev et al., 2013), including the cardio – vascular (Vaya et al., 2013) and cerebrovascular diseases (Szapary et al., 2004); this may complicate the course of these diseases (Plotnikov et al., 2005). This experiment was conducted with a view to show the impact of potassium dichromate on blood rheology and to explore the impact of boric acid on the morpho-functional disturbances of erythrocyte membranes, induced by hexavalent chromium (K₂Cr₂O₇). In this study, EDI decrease and EAC increase, peroxide hemolysis and erythrocyte osmotic fragility reflects deterioration of blood rheological properties. Intake of boric acid in the presence of chromium-induced diselementosis improves blood rheological properties (EDI, Ht, EAC, EPG and EOF). Perhaps, changes in blood rheological properties is associated with antioxidant activity of boron compounds (indirect action). Thus, a number of scientists found that boron compounds (boric acid, borax, etc.) could be used for correcting metal-induced (arsenic, bismuth, cadmium, mercury, lead) oxidative stress effects (Turkez et al., 2007; Kucukkurt et al., 2015; Pawa and Ali, 2006). Indeed, oxidative stress occurs at the decreased level of antioxidants (Tapiero et al., 2004). Boric acid intake contributes to the preservation of pro-oxidant – antioxidant balance (Bolanos et al., 2004; Turkez, 2008; Inse et al., 2010).

Possibly, there is another mechanism – the direct and immediate impact, which implies hematopoiesis disorders under the influence of boron compounds, which, in turn, influence physical and chemical blood properties (Oto et al., 2015). Boron in low doses (40 and 80 mg / l) can stimulate erythropoiesis and hemoglobin synthesis (Feng et al., 2014) having protective effect in the presence of metal-induced hemorheology disorders (Turkez et al., 2012).  Boron in high doses (160 – 640 mg / l) can inhibit hematopoiesis and hemoglobin synthesis; i.e., it can be toxic (Feng et al., 2014).

Thus, the authors of the present study first found that boric acid in the presence of chromium-induced hemorheology disorders prevents (hinders) blood rheology disorders in rats (showing protective action). Apparently, taken in certain doses, boric acid is a promising remedy in the case of chromium-induced hemorheology disorders and suggests new dimensions of subsequent studies related to the biological effects of boron compounds. In the presence of chromium-induced disorders (diselementosis) in rats, one can observe changes in blood rheological properties – erythrocyte deformability index and the level of hematocrit are reduced on the background of increase in EAC, peroxide hemolysis and erythrocyte osmotic fragility. Oral administration of boric acid on the background of chromium-induced changes in the blood rheological properties inhibits the development of blood cell disorders (protective action).

Conclusion

Implications and recommendations for future studies are as follows: Some studies show that Cr⁺⁶  and its compounds do not directly generate free radicals; however, reduction of Cr⁺⁶  to Cr⁺³, as well as the effect of Haber – Weiss and Fenton mechanisms (Lloid et al., 1998), imply the emergence of different radicals that cause damages characteristic of oxidative stress (Pritchard et al., 2000; Barrera et al., 2003). Consequently, one of the possible basic approaches used for prevention (correction) of K₂Cr₂O₇ – induced damage implies using agents (elements) with powerful antioxidant properties. Recent studies have shown that boron and its compounds displayed significant protective effects against damages induced by metals, such as aluminum and arsenic (Turkez et al., 2011; Kucukkurt et al., 2015). Erythrocytes are sensitive to the impact of heavy metals, including chromium compounds (Ryspekova et al., 2013); they present a convenient model to assess cytotoxicity of chemicals. Hemolysis and its value may serve as a stability test for cell membranes of erythrocytes (Pagano and Faggio, 2015). The latter are characterized by certain rheological properties (deformation and flow), due to which they have a biconcave shape, high flexibility, elasticity and deformability. Deterioration of their rheological properties trigger the occurrence of hemorheology disorders in the presence of various diseases (Muraviov and Tikhomirova, 2009; Sharapova, 2012; Baev et al., 2013), including the cardio – vascular (Vaya et al., 2013) and cerebrovascular diseases (Szapary et al., 2004); this may complicate the course of these diseases (Plotnikov et al., 2005). This experiment was conducted with a view to show the impact of potassium dichromate on blood rheology and to explore the impact of boric acid on the morpho-functional disturbances of erythrocyte membranes, induced by hexavalent chromium (K₂Cr₂O₇). In this study, EDI decrease and EAC increase, peroxide hemolysis and erythrocyte osmotic fragility reflects deterioration of blood rheological properties. Intake of boric acid in the presence of chromium-induced diselementosis improves blood rheological properties (EDI, Ht, EAC, EPG and EOF).

References

1. Krivokhizhina LV, Zinger V.F and Kantyukova S. A. Erythron, its qualitative and quantitative changes under the impact of industrial chemicals. Bulletin of South Ural State University. 2006;1(7):119-120.
2. Khetsuriani R.G and Kipiani V.A.  Morphofunctional changes in the red blood cells in the aging process. J. Immunorehabil. 2002;2:214-221.
3. Lukyanova L.D.  Molecular mechanisms of tissue hypoxia and organism adaptation. Physiological magazine. 2003;3:17-35.
4. Lowe G.D, Lee A.J, Price J.F and Fowkes F.G.  Blood viscocity and risk of cardiovascular events: The Edinburgh Artery Study. J. Haemotology. 1997;96:168-73.
   CrossRef
5. Szapary L, Horvath B, Marton Z, Alexy T, Demeter N, Szots M, Klabuzai  A, Kesmarky G, Juricskay I, Gaal V, Czopf J and Toth K. Hemorheological disturbances in patients with chronic cerebrovascular diseases. Hemorheol. Micro. 2004;31:1-9.
6. Ormotsadze G and Nadareishvili K.  A new method studying the red blood system. Radiation studies. 2002;1:5–36.
7. Gyawali P, Richards R.S, Bwititi P.T and Nwose E.U.  Association of abnormal erythrocyte morphology with oxidative stress and inflammation in metabolic syndrome. Blood Cells. Mol. Dis. 2015;54(4):360–363.
   CrossRef
8. Zairova N.S, Arifkhanova S.I and Pogoreltsov V.I.  Modification of erythrocyte membranes by perfluorates in the presence of papain emphysema in rats. Pathological physiology and experimental therapy. 2006;1:18-20.
9. Kotelnikov A.V and Kotelnikova S.V. Peroxide resistance of erythrocytes in white rats at norm and during intake of vitamin E at different stages of ontogenesis. Bulletin of Russian Peoples’ Friendship University. 2005;2(129):131.
10. Zimetti F, Weibel G.K and Duong M.N. Measurement of cholesterol bidirectional flux between cells and lipoproteins. Lipid Res. 2006;3:605-613.
    CrossRef
11. Pagano M and Faggio C. The use of erythrocyte fragility to assess xenobiotic cytotoxicity. Biochem. Funct. 2015;33(6):351-5.
    CrossRef
12. Kawanish S, Hiraku Y, Murata M and Okawa S. The role of metals in site – specific DNA damage with reference to carcinogenesis. Free Radical. Biol. Med. 2002;32:822–832.
13. O’Brien T.J, Ceryak S and Patierno S.R. Complexities of chromium carcinogenesis: role of cellular response, repair and recovery mechanisme. Res. 2003;533:3–36.
    CrossRef
14. Bazarbekova S.K. Cardiotoxic effects of chromium and ways of their correction. Thesis synopsis. Almaty. 2002;23c.
15. Sudha S, Kripa S.K, Shibily P, Shyn J. Elevated Frequencies of Micronuclei and other Nuclear Abnormalities of Chrome Plating Workers Occupationally Exposed to Hexavalent Chromium. Iran J. Cancer. Prev. 2011;3:119-124.
16. Stout M.D, Herbert R.A, Kissling G.E, Collins B.J, Travios G.S, Witt K.L, Melnick R.L, Kamal M, Abdo D, Malarkey E and Hooth M.J.  Hexavalent Chromium Is Carcinogenic to F344/N Rats and B6C3F1 Mice after Chronic Oral Exposure. E Health Perspect. 2009;117(5):716-722.
    CrossRef
17. Aruldhas M.M, Subramanian S, Sekhar P, Vengatesh G, Chandrahasan G, Govindarajulu P and Akbarsha M.A.  Chronic chromium exposure – induced changes in testicular histoarchitecture are associated with oxidative stress: study in non – human primate (Macaca radiate Geoffroy). Reprod. 2005;20(10):2801-2813.
    CrossRef
18. Wise S.S, Holme A.L and Wise J.P.S.H. Hexavalent chromium – induced DNA damage and repair mechanisms. Environ. Health. 2008;23(1):39-57.
    CrossRef
19. Wang X, Son Y.O, Chang Q, Sun L, Hitron J.A, Budhraja A, Zhang Z.h, Ke Z, Chen F, Luo J, Shi X.  NADPH Oxidase Activation Is Required in Reactive Oxygen Species Generation and Cell Transformation Induced by Hexavalent Chromium. Toxicol Sci. 2011;123(2):399-410.
    CrossRef
20. Iztleuov M.K. Homeostasis and chromic pathology. Aktobe. 2003.
21. Iztleuov E.M, Zharmakhanova G.M and Iztleuov M.K. The impact of “Shukurmay” oleum polyphytum on chromium-induced mutations in somatic cells and on oxidative stress. Topical issues of physiology, medicine, and education. Almaty. 2011.
22. Solis-Heredia M.J, Quintanilla-Vega B and Sierra-Santoyo A, Hernandez J.M, Brambila E, Cebrian M.E, Albores  A. Chromium increases pancreatic metallothionein in the rat. Toxicology. 2000;142:111–117.
    CrossRef
23. Bagchi D, Balmoori J, Bagchi M, Ye X, Williams C.B, Stohs S.J. Comparative effects of TCDD, endrin, naphthalene and chromium VI on oxidative stress and tissue damage in the liver and brain tissues of mice. Toxicology. 2002;175:73-82.
    CrossRef
24. Fatima S, Arivarasu N.A, Banday A.A, Yusufi A.N.K, Mahmood R. Effect of potassium dichromate on renal brush border membrane enzymes and phosphate transport in rats. Exp. Toxicol. 2005;21:631-638.
    CrossRef
25. Mamyrbayev A.A. Toxicology of chromium and its compounds. Aktobe. 2012-284.
26. Hunt C.D. Regulation of enzymatic activity: one possible role of dietary boron in higher animals and humans. Trace. Elem Res. 1998;66:205–225.
    CrossRef
27. Turkez H, Geyikoglu F, Tatar A, Keles S and Ozkan A. Effects of some boron compounds on peripheral human blood. Naturforsch C. 2007;62:889-896.
    CrossRef
28. Hu Q.I, Li S, Qiao E, Tang Z, Jin E, Jin G and Gu Y.  Effect of boron on structure and antioxidative activities of spleen in rats. Trace Elem. Res. 2014;158(1):73–80.
    CrossRef
29. Barronco W.T, Kim D.H, Stell S.L and Eckhert C.D. Boric acid inhibits stored Ca+2 release in DU –145 prostate cancer cells. Cell Biol. 2008;25:309–320.
30. Ornat S.T and Konur M. Cytogenetic Evaluations of Peripheral Blood Samples of Boron Workers. In proceeding of the 2nd International Boron Symposium Eshisehir, Turkey. 2004.
31. Oto G, Arihan O, Celikezen F.C,  Yildiray m.B, Semra S.  Effect of doxorubicin and some boron compounds on erythrocyte fragility in rats. Sci. Disc. 2015;1(2):50–3.
    CrossRef
32. Iztleuov M.K. Pathogenesis of homeostasis disorders, caused by excessive chromium intake and ways of their correction. Doctoral thesis. Moscow. 2004.
33. Tapiero H, Townsend D.M and Tew K.D. The role of carotenoids in the prevention of human pathologies. Pharmacother. 2004;58:100-110.
    CrossRef
34. Valko M, Morris H and Cronin M.T.  Metals, toxicity and oxidative stress. Med. Chem. 2005;12:1161–1208.
    CrossRef
35. Bolanos L, Lukaszewski K, Bonilla I and Blevins D.  Why boron? Physiol. Biochem. 2004;42:907–912.
    CrossRef
36. Turkez H. Effect of boric acid and borax on titanium dioxide genotoxicity. Appl. Toxicol. 2008;28:658-664.
    CrossRef
37. Strasburg: Council of Europe. European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes. 1986.
38. Moore J.A. Expert Scientific Committee. An assessment of boric acid and borax using the IEHR evaluative process for assessing human developmental and reproductive toxicity of agents. Toxicol. 1997;11:123–160.
    CrossRef
39. Pahl M.V, Culver B.D and Vaziri N.D. Boron and the kidney. Ren. Nutr. 2005;15:362-370.
    CrossRef
40. Zakharova N.B, Tselik N.I and Klyachkin M.L. Methods of erythrocyte deformability studies. Health Care of Belarus. 1989;7:19-21.
41. Lapotnikov V and Kharash L.M.  A simple method used to determine the circulating erythrocyte aggregates. Laboratory business. 1982;7:5-7.
42. Pokrovskiy A.A and Abrarov A.A.  On the question of peroxide resistance of erythrocytes. Nutrition. 1964;6:44-49.
43. Todorov J. Clinical laboratory studies in pediatrics. Sofia: Health and Physical Education. 1968.
44. Bielicka A, Bojanowska I, Wisniewski A. Two Faces of Chromium – Pollutant and Bioelement. J.  Environ. Stud. 2005;14(1):5–10.
45. Lloid D.R, Carmichael P.L and Phillips D.N. Comparison of the formation of 8 – hydroxi – 2 – deoxyguanosione and singl – and doubl – strand breaks in DNA mediated by Fenton reaction. Res. Toxical. 1998;11:420–427.
    CrossRef
46. Pritchard K.A, Ackerman A and Kalyanaraman B. Chromium VI increases endothelial cell expression of ICAM – 1 and decreases nitric oxide activity. J. Environ. Pathol. Toxicol. Oncol. 2000;19:251-260.
47. Barrera D, Maldonado P.D, Medina-Campos O.N, Hemandez-Pando R, Ibarro-Rubio M.E and Pedraza-Chaverri J.   HO – 1 induction attenuates renal damage and oxidative stress induced by K2Cr2O7. Free Radical. Biol. Ved. 2003;34:1390-1398.
48. Turkez H, Geyikoglu F and Colak S.  The protective effect of boric acid on aluminum – induced hepatotoxicity and genotoxicity in rats. J. Biol. 2011;35:293-301.
49. Kucukkurt I, Ince S, Demirel H.H, Turkmen R, Akbel E and Сerik Y.  The Effect of Boron on Arsenic – induced Lipid Peroxidation and Antioxidant Status in Male and Female Rats. Biochem. Mol. Toxicol. 2015;29:564-571.
50. Ryspekova N.N, Nurmagambetov A.N, Askarova A.E and Akhanov A.A. The role of heavy metals in the development of anemia (review). Bulletin of KazNMU. 2013;3(2):46-51.
51. Muraviov A.V and Tikhomirova I.A.  Evaluation of haemorheologic status and microcirculation in healthy individuals and in patients with hypertension. Regional circulation and microcirculation. 2009;3:37-42.
52. Sharapova N.V.  Blood viscosity, relaxation time and blood stress in patients with community-acquired pneumonia of varying severity. The world of science.culture and education. 2012;1(32):252-254.
53. Baev V.M, Sharapova N.V and Shmeleva S.A. Blood rheology restoration in patients with community-acquired pneumonia on the background of arterial hypertension in the hospital therapy. Pathological Physiology and Experimental Therapy. 2013;2:23-25.
54. Vaya A, Alis R, Romagnoli M, Perez R, Bautista D, Alonso R and Laiz B. Rheological blood behavior is not only influenced by cardiovascular risk factors but also by aging itself. Research into 927 healthy Spanish Mediterranean subjects. Hemorheol. Micro. 2013;54:287-296.
55. Plotnikov M.B, Yamkin A.V, Aliev O.I and Tyukavkina N.A.  The impact of a complex of acetylsalicylic acid and DIQUERTIN on platelet aggregation and hemorheological indices in rats with cerebral ischemia. Clin. Pharmacol. 2005;68(2):33-35.
56. Pawa S and Ali S. Boron ameliorates fulminant hepatic failure by counteracting the changes associated with oxidative stress. Boil. Interact. 2006;160:89-98.
    CrossRef
57. Inse S, Kucukkurt I, Cigerci I.H, Fidan A.F and Eryavuz A. The effects of dietary boric acid and borax supplementation on lipid peroxidation, antioxidant activity, and DNA damage in rats. Trace Elem. Med. Bio. 2010;24(3):161–164.
    CrossRef
58. Feng B, Li X, Li S and Wang J. Effects of boron on structure and antioxidative activities of spleen in rats. Trace Elem. Res. 2014;158(1):73-80.
    CrossRef
59. Turkez H, Geyikoglu F, Tatar A, Keles M.S and Kaplan I. The effects of some boron compounds against heavy metal toxicity in human blood. Exp. Toxicol. Pathol. 2012;64:93-101.
    CrossRef