eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2017-12-21 10 4 1765 1771 10.13005/bpj/1290 17537 article Dewa Putu Gede Purwa Samatra 1,2 Mahadewa Tjokorda G. B. 2 I Dewa Made Sukrama 2,3 Ni Wayan Sucindra Dewi 4 Rian Ka Praja 5 Dian Nurmansyah 5 Neurology Deparment, Faculty of Medicine, Udayana University, Bali, Indonesia. Doctoral Program of Biomedical Science, Faculty of Medicine, Udayana University, Bali, Indonesia. Clinical Microbiology Department, Faculty of Medicine, Udayana University, Bali, Indonesia. Pharmacology Deparment, Faculty of Medicine, Udayana University, Bali, Indonesia. 5Master Degree of Biomedical Science, Udayana University, Bali, Indonesia. Salmonella typhi infection in typhoid fever can produce oxidative stress. Earthworm, as an herbal medicine, nowadays is widely used to treat cases of typhoid fever. This study aimed to prove the antioxidant effect of earthworm extract (Lumbricus rubellus) in reducing the levels of malondialdehyde (MDA) and 8-hydroxy-deoxyguanosine (8-OHdG) in male wistar rats infected by S. typhi. The research was conducted using posttest-only control group design method with 28 samples divided into 4 treatment groups: the negative control group (not S. typhi infected), positive control (only S. typhi infected), treatment group 1 (infected with S. typhi on day 1 and giving L. rubellus earthworm extract on the next day until day 18) and treatment group 2 (giving L. rubellus earthworm extract in the first week then infected with S. typhi on day 8, followed by extract until day 18^th). On the 18^th day, blood samples were taken for further measurement of MDA and 8-OHdG levels. Based on the results of statistical analysis, treatment group 1 was not able to significantly decrease MDA levels (p> 0.05) with mean of 4.38 ± 0.38 nmol/mL, while the treatment group 2 significantly decreased MDA levels (p < 0.05) with a mean of 3.54 ± 0.19 nmol/mL. In addition, treatment 1 and 2 significantly decreased levels of 8-OHdG (p <0.05) with mean treatment rates of 0.86 ± 0.08 ng / mL and mean treatment of 2 0,52 ± 0.07 ng / mL. Based on the results, it can be concluded that the earthworm extract (Lumbricus rubellus) can reduce levels of MDA and 8-OHdG in male wistar rats infected by S. typhi. https://biomedpharmajournal.org/vol10no4/extract-of-earthworms-lumbricus-rubellus-reduced-malondialdehyde-and-8-hydroxy-deoxyguanosine-level-in-male-wistar-rats-infected-by-salmonella-typhi/ Earthworm Lumbricus rubellus malondialdehyde 8-hydroxy-deoxyguanosine  Salmonella typhi