eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2017-09-25 10 3 1507 1515 10.13005/bpj/1260 16268 article Panigrahy Ankita 1 Behera Jayanti Prava 1 Priyadarshi Kunal 1 Maharana Chandra Sekhar 1 Department of Pharmacology, M.K.C.G Medical College, Berhampur,Odisha, India Pin 760004. Diabetes mellitus is often associated with several organ system complications like retinopathy, neuropathy, nephropathy etc. Patients also have alterations in sexual functions i.e decrease in libido, fertility. Pedalium murex, a herb found in India is known to possess hypoglycemic, antioxidant, aphrodisiac properties. This study evaluated the effect of petroleum ether extracts of P.murex dry seed (PEPM) on testicular dysfunction by estimating fasting blood glucose, oxidative stress markers, seminal analysis and histopathological study of testis in streptozotocin induced Type 2 diabetes mellitus (T2DM). Thirty wistar albino rats were randomly divided into 5 groups (n=6). Group1 (control), group-2 to group-5 were treated with single dose injection (i.p) of streptozotocin (40mg/kg) for induction of diabetes mellitus. All the rats received different drugs for 60 days as follows:  Group 1(Tween 80) Group2: Disease Control (Tween 80), Group3- metformin (50 mg/kg), Group 4-PEPM (400 mg/kg) and Group 5-(PEPM)+ metformin (25mg/kg). On day 61, rats were sacrificed and FBG estimated, semen collected from caudal epididymis. Sperm count, motility, morphological abnormalities were noted by microscopic examination. Oxidative stress markers like MDA and SOD were estimated from testicular tissue homogenate.  Histopathological examination of testis was done and scored by Johnsen’s score. All the data were analysed by One way ANOVA except Johnsen’s score which was analysed by Kruskal-Wallis test . P< 0.05 was considered significant. The normal rats received PEPM produced no significant change in FBG showing no effect in normoglycemia.Diabetic control rats showed significant increase in FBG, alteration in testicular MDA level and SOD activity, decrease in sperm parameters and Johnsen’s score. PEPM(400 mg/kg) and combination of PEPM+ metformin treated groups significantly reversed (p <0.01) the above parameters. Phytochemicals present in P.murex possess anti-hyperglycemic, germ-cell protective and antioxidant properties. So it can be suggested further studies to be conducted to evaluate the role of PEPM in both male and female reproductive impairment due to diabetes can throw further light in this area. https://biomedpharmajournal.org/vol10no3/effect-of-pedalium-murex-dry-seed-extract-on-testicular-dysfunction-in-streptozocin-induced-diabetes-mellitus-in-wistar-albino-rats/ PEPM Johnsen’s score Oxidative stress Sperm analysis