eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2017-06-20 10 2 675 682 10.13005/bpj/1155 15346 article Nitya Rudraraju 1,2 Animisha Mokkapati 1 Radhakrishna Nagumantri 1 Chinna Babu Pydi 1 Ramakrishna Chintala 3 Satyanarayana Rentala 1 Department of Biotechnology, Institute of Technology, GITAM University, Gandhi Nagar, Visakhapatnam 530 045 Indai. School of Engineering, Boston University, Massachusetts USA. GITAM Institute of Science, GITAM University, Gandhi Nagar, Visakhapatnam 530 045. A daily diet rich in spices may offer protection against cancer and other illnesses. This may be reason, why Indians suffer lower cases of many cancers. One of the most widely traded spices in the world, Piperine, the main alkaloid from black pepper has been shown to substantially increase the bioavailability of the nutrients in foods and supplements. Piperic acid is a chemical obtained by the hydrolysis of the alkaloid piperine from black pepper, followed by acidification of the corresponding salt. The cytotoxic effects of piperic acid are hither to unknown. In this paper, the cytotoxic effects of piperic acid in prostate cancer cells (PC-3) and breast cancer cells (MDA-MB-231) were studied. The drug treatment experiments clearly indicated that maximum cytotoxicity was achieved at 48 hours and at 100µM concentration of piperic acid in both the cell lines. https://biomedpharmajournal.org/vol10no2/in-vitro-evaluation-of-cytotoxic-properties-of-piperic-acid/ Piperic acid Cytotoxicity Prostate cancer cell lines Breast cancer cell lines and Mononuclear cells