Vladimirovich Z. V, Nikolaevna M. O, Vasil'evna M. G, Vladimirovich S. A, Mihajlovich U. A, Vladimirovich T. V.Hemostasis and Rheological Blood Features Dynamics of Black-Many Coloured Lactating Cows at the Inclusion into their Ration of Antioxidant Lipisomal Preparation "Lipovitam-Beta". Biomed Pharmacol J 2017;10(2).

---

Manuscript received on :February 26, 2017
Manuscript accepted on : March 23, 2017
Published online on: --

---

Plagiarism Check: Yes

How to Citeclose    |   Publication History close

 Views: (Visited 531 times, 1 visits today)    PDF Downloads: 717

Zaitsev Vladimir Vladimirovich¹, Makurina Ol'ga Nikolaevna², Molyanova Galina Vasil'evna¹, Savinkov Aleksej Vladimirovich¹, Uhtverov Andrej Mihajlovich¹ and Tarabrin Vasilij Vladimirovich¹  

¹Federal State Budgetary Educational Institution of Higher Education “Samara State Agricultural Academy”, Samara, Russia.

²Samara National Research University, Samara, Russia.

Corresponding Author E-mail: zaitsevkinel@mail.ru

DOI : https://dx.doi.org/10.13005/bpj/1165

Abstract

One of modern vitamin sources for lactating cows is Lipovitam-beta. It was made on liposomes' basis and can provide maximum vitamins' assimilation by the whole organism's cells. Aim of investigation: to estimate Lipovitam-beta impact on the indices of hemostasis system and blood rheology of high-productive cows during the first 5 months of lactation. We examined 207 healthy black-many coloured cows having begun the process of lactation. They were of the 3-4th calving and were kept in conditions of fastened support having traditional ration. The animals were casually divided into experimental and control groups. in the experimental group (104 cows) we examined the impact of Lipovitam-beta feeding on the basis of 1 capsule (0,17gr) on 30 kg of body mass once in 5 days during the first 5 months of lactation on accountable indices. The control group was composed of 103 lactating cows. We estimated the basic indices of hemostasis, blood viscosity, erythrocytes' aggregation and deformability on the 5th day and in 5 months of lactation. Lipovitam-beta application led in case of cows from experimental group to reliable decrease of spontaneous and stimulated platelets' aggregation and weakening of plasma hemostasis activity. On the background of Lipovitam-beta feeding the cows were found to have decrease of blood viscosity and erythrocytes' aggregation index what was accompanied by increase of erythrocytes' deformability index. The cows from the control group during the first 5 months of lactation were noted to have rise of platelets' aggregation and increase of hemocoagulation. It was accompanied by the tendency to increasing of blood viscosity and erythrocytes' aggregation with inclination to the decrease of their deformability. High-productive cows by the middle of lactation are noted to have developing tendency to hemostasis activation and worsening of blood rheological features what weakens microcirculation processes and may be one of the factors gradually blocking lactation. Lipovitam-beta application to lactating cows led to activity decrease of plasma hemostasis and platelets, increase of rheological features what can positively influence microcirculation and the level of dairy producing capasity.

Keywords

Cows; lactation; Lipovitam-beta; hemocoagulation; platelets; blood rheology

Introduction

At present cattle breeding is one of very significant branches of modern agriculture providing population with dairy products of full value and dietary meat.^1,2 Nowadays in the whole world the task of its intensification is established. It should be realized at the expense of  high-productive livestock growth and application of progressive feeding technologies and keeping of lactating cows.³  Science gradually comes to understanding that great significance for the provision of vitality and productive capasity of cattle belongs to the state of one of its integrating systems – blood.^4,5 Its hemostatic and rheological features mostly define the degree of tissues’ perfusion and, consequently, anabolism level in an animal’s organism and its productive characteristics at any age.^5,6 Because of great physiological significance and vulnerability of hemostasis and blood rheology they are started to be investigated more and more actively.⁷

One of valid ways causing intensification of anabolic processes in an organism and, thereby, increase of phenotypic manifestation of genetically conditional features^8,9 is application of different biologically significant impacts^10,11 and biologically active substances.¹² In their number we include rise of physical activity¹³ and also application of vitamins. The need in some vitamins in case of high-productive cows is very high and with the help of ration can’t sometimes be covered entirely.⁵ One of modern vitamin sources for lactating cows is Lipovitam-beta which, being made on the basis of liposomes, can provide maximum vitamins’ assimilation by an organism’s cells.¹⁴

It is known that blood – is the most labile indicator of an organism’s state quickly reacting on different ingredients’ inflow.^15,16 The more metabolism in an organism increases under their impact, the more evident will be changes in blood.^17,18 Besides, blood changing its composition, hemostatic and rheological features, can itself influence the functional state of the whole organism.^19,20 In this connection we considered to be approved to fulfil estimation of Lipovitam-beta impact in case of high-productive lactating cows on physiologically significant indices of hemostasis and blood rheology.

Aim of investigation: to estimate Lipovitam-beta impact on the indices of hemostasis system and blood rheology of high-productive cows during the first 5 months of lactation.

Materials and Methods

In our investigation we took 207 healthy black-many coloured cows of pure breed of 3-4th calving being kept at the farms of Samarsk region having given during the previous lactation 7,5-8 thousand of litres of milk each. The cows were taken under investigation on the 5th day of lactation. All the cows were kept in conditions of fastened support and were milked thrice. The ration of taken into investigation lactating cows was composed of corn silage, wheat straw, schrot of sunflower, fodder syrup, mixture of concentrates. The animals were casually divided into experimental and control groups. The experimental group was composed of 104 cows who additionally received Lipovitam-beta (“Biodom”, Russia) on the basis of one capsule (0,17gr) on 30 kg of animal’s body mass by mixing it with concentrates. The preparation was given once in 5 days during the first 5 months of lactation. The control group was composed of 103 lactating cows of similar age being kept in similar conditions and receiving the same ration. The examination of animals in both groups was fulfilled twice: on the 5th day after calving and in 5 months of lactation.

All the cows were examined with the object to the content of fibrinogen in blood by Klause’ modified method.²¹ Estimation of plasminogen level was defined by kinetic method with the help of the device FP-901 (“LabSystems”, Finland) with chromogen substrates (“Dade Behring”, Germany). The concentration of soluble fibrin-monomeric complexes (SFMC) was defined by visual method with the help of reagents by the firm “Technology-standard”, Russia.²¹ Activated partial thromboplastin time (APTT) was examined with the help of coagulometre “HumaClot” (“HUMAN GmbH”, Germany) with a set of reagents HemoStat aPTT-EL. The definition of international normalized relation (INR) was made by Quick’s method.²¹ Platelets’ aggregative ability was examined with the help of two-channel laser analyzer of platelets’ aggregation (“Biola”, Russia) by turbo-dymetric method. As inductor we applied 0,5mkM ADP.²²

Registration of blood viscosity was fulfilled by rotary viscosimeter AKP-2 (“Melt”, Russia) at shift speeds 200 sec^-1 and 20 sec^-1 with the consequent calculation of erythrocytes’ aggregation index and deformability index.²² Statistical processing of the received results was made with the help of Student’s t-criterion.

Results

During the first 5 accountable months of lactation the cows, having received Lipovitam-beta, gave on average 4420,0±17,4 kg of milk each (3,8±0,03% fat content), while the cows from the control group gave on average 4140,0±27,7 kg of milk each (3,9±0,04% fat content).

Estimation of hemostasis state on the 5th day of lactation in cows of both groups didn’t find reliable differences in values of defined indices of coagulant and anticoagulative systems (table). Platelets’ functional activity before the start of investigation also had no differences in both groups of animals.

Table 1: Indices’ dynamics of hemostasis and blood rheology of lactating cows

Сonventions: reliability of indices’ dynamics * – p˂0,05, ** -p˂0,01.

At the beginning of investigation we  also didn’t find any reliable differences between both groups of animals as far as indices of blood rheological features are concerned. Blood viscosity at shift speed 200 sec^-1 in the group which later began to receive Lipovitam-beta was equal to 4,27±0,19 centipoise, in the control group – 4,29±0,22 centipoise. At shift speed 20 sec^-1 the values of blood viscosity were also comparable composing 6,30±0,35 and 6,28±0,38 centipoise, correspondingly. Initially indices’ values of erythrocytes’ deformability and erythrocytes’ aggregation of animals in both groups statistically didn’t differ from each other.

To the end of investigation period the cows receiving Lipovitam-beta were noted to have reliable inhibition of APTT, tendency to increasing of INR and plasminogen indices, decrease of fibrinogen and SFMC concentrations. In the control group of cows in 5 months of lactation we noticed increase of APTT, tendency to decreasing of INR and plasminogen activity at increase in blood of fibrinogen and SFMC.

In 5 months cows from experimental group were noted to have decrease of platelets’ aggregation – spontaneous on 11,5% – stimulated on 11,8%. In the control group of lactating cows spontaneous and ADP-inducible platelets’ aggregation reliably rose, having exceeded initial values on 14,7% and 24,1%, correspondingly. At the same time differences in platelets’ aggregation in both groups to the end of investigation composed for spontaneous – 26,9% (p˂0,01), for stimulated – 39,4% (p˂0,01).

At repeated investigation of accountable indices of blood rheological features in 5 months of lactation we noticed multidirectional tendency of blood viscosity common for animals of both groups. The cows from experimental group were noted to have tendency to decrease of blood viscosity at shift speeds 20 sec^-1 and 200 sec^-1 on 5,2% and 6,1%, correspondingly. Given indices of control animals showed tendency to growth on 3,0% and 3,8%, correspondingly. Experimental cows were also noted to have tendency to value decrease of erythrocytes’ aggregation index on 4,0%, and control animals were found to have tendency to its increase on 4,7%. At the same time index of erythrocytes’ deformability of lactating cows having received Lipovitam-beta had tendency to increase and in case of control animals had tendency to decrease.

Discussions

Being clear genetically coded all the features of a living organism^23,24 can change the degree of their phenotypic survival depending on the environment.²⁵ In this connection high urgency of profound investigation continuation of living organisms’ physiology different aspects²⁶ and a human being is still kept, including negative environmental conditions^27,28 with obligatory registration of their social consequences.²⁹ In this connection additional investigations devoted to calves ‘ and cows’ physiology can provide solid basis for further perfection of their keeping and feeding technologies.^1,3 As the result of summation of received during these investigations knowledges and their consequent practical application we can reach intensification of cattle breeding.^2,5

In previous investigations they showed with the help of different biological objects that hemostasis^30,31 and hemorheology^32,33 rather keenly respond to environmental impacts including unfavourable factors³⁴ and development of different dysfunctions³⁵ and evident pathology^15,16 in an organism. It is also known that inhibition of lipid peroxidation and suppression of lack of vitamins can influence a living organism rather variously and favourably.³⁸ It was noted that on this background activity decrease of many components of hemostasis system³⁹ and improvement of blood rheological parameters on the whole and erythrocytes in particular^40,41 are developing. With these very changes of hematological indices we connect improvement of micricirculation processes and intensification of metabolic processes on the background of different varients’ impacts on an organism having in its basis antioxidant impact.⁴²

As the result of fulfilled investigation on the background of Lipovitam-beta application the cows were noted to have weakening of hemostasis activity and improvement of blood rheology. At the same time control results showed reverse regularity having provided to the end of investigation reliability of different accountable indices in both groups of animals.

Received results allow us to consider that additional inflow of balanced vitamin complex into an organism of a lactating cow weakens the process of hemocoagulation along both ways of its realization.²¹ It is evidently connected with activity decrease of most coagulation factors participating in it. Most probably that some decrease of thromboplastin generation and weakening of XII factor’s contact activation were also developing in these animals’ blood. Lipovitam-beta addition into animals’ feeding led also to decrease of fibrinogen and SFMC in blood what pointed at inhibition of its polymerization which was actively restrained by physiologically activated system of fibrinolysis. Lactating control cows were noted to have opposite phenomena promoting increase of all the hemocoagulation stages and decrease of fibrinolysis activity.

Relying on literature data we can consider that Lipovitam-beta application stimulates organism’s antioxidant protection,^12,14 what, as it is known, decreases platelets’ ability to both spontaneous and stimulated aggregation.^43,44 We have some basis to think that addition of the given preparation into animals’ feeding rises their level of cyclic adenosine monophosphate in platelets and decreases thromboxan A₂ formation. Given situation blocks the formation of thrombocyte aggregates in the lumen of the vascular bed.^45,46 Noted in control cows aptitude to the growth of platelets’ aggregative ability may be connected with the decrease of cyclic adenosine monophosphate in them⁴⁷ and increase of thromboxan A₂ synthesis in them,^48,49 naturally leading to increase of platelets’ dynamic aggregates’ number in these animals’ blood.^50,51,52

Found in control cows increase of erythrocytes’ aggregation can mostly be provided by coming changes of their membranes’ charge because of degradation on their surface of some glycoproteins under the influence of  always strengthening in conditions of low physical activity lipid peroxidation.³⁵ Intensification of oxygen active forms’ generation in given conditions provides oxidative alteration of erythrocyte membranes’ structures in these animals⁵³ at simultaneous damage of plasma globular proteins having the ability to be connected in the kind of “bridges” between erythrocytes and realize their aggregation.⁵⁴ Given situation promotes the growth of disaggregation threshold of control cows’ red platelets.⁵⁵

It can be supposed that found in experimental cows weakening of erythrocytes’ aggregation is mostly provided by strengthening of antioxidant plasma activity as the result of Lipovitam-beta application. On this background we noticed decrease of α₁-receptors activity, weakening of Ca²⁺-calmodulin system and cascade of phosphatidyl inositol intracellular reactions.⁵⁶ Taking place at this time decrease of α₂-adrenoreceptors’ activity leads to strengthening of adenylate cyclase during physiological impacts from receptors on Gi-proteins what causes rise of cyclic adenosine monophosphate quantity in erythrocytes, blocks Ca²⁺ inflow into them, providing not high erythrocytes’ aggregation.^40,53

We can consider that erythrocytes of animals having received Lipovitam-beta are more able to deformation what is an important factor of maintenance in them of necessary microcirculatory bed’s perfusion. It is possible that in these animals’ erythrocytes there is rise of ATP content what positively influences the interaction of spectrin, actin and other integral proteins of the erythrocyte membrane playing an important role in its features’ maintenance. Besides,  rise of erythrocytes’ deformability in experimental cows is mostly explained by lowering of unbounded ions Ca²⁺ concentration what minimizes its interaction with membrane’s proteins and makes it more deformed. At the same time in erythrocytes of control animals there is evidently lowering of ATP content what negatively changes the character of interaction of spectrin, actin and erythrocyte membrane’s integral proteins. Erythrocytes’ deformability lowering in control cows is evidently mostly caused by the rise of unbounded ions Ca²⁺  level  in them which interacting with membrane’s proteins make it firmer and less deformed.⁴⁰

In modern literature the opinion is gradually forming about close connection of somatic status and animals’ producing capacity with their hematological indices.^3,57 At the same time in this investigation we didn’t trace the whole lactation and, that’s why it’s too early to make conclusions about the impact of Lipovitam-beta feeding on lactation level trying to explain the results from the position of hemostasis activity dynamics and state of blood rheology. At the same time, found tendency to higher level of dairy productive capacity at more physiologically profitable state of accountable indices makes us think that such dependence can be found in future investigations.

Conclusion

High-productive black-many coloured cows to the middle of lactation get the tendency to hemostasis activation and worsening of blood rheological features what negatively influences microcirculation and can become one of the factors gradually inhibiting lactation. Lipovitam-beta application to lactating cows led to decrease of plasma hemostasis and platelets’ functional activity, weakening of erythrocytes’ aggregative ability, strengthening of their deformability, improvement of blood fluidity at different shift speeds what can positively influence microcirculation and level of dairy producing capacity.

Conflict Of Interest

No conflict of interest to declare.

References

1. Belkov G. I., Panin V. A. Indices of the milk productivity of the cross-breeds of the obtained from the crossing simmenthal cows with the bulls of the Holstein species of different populations Tyapugin. Russian Agricultural Sciences. 2015;3:47-49.
2. Yamid F., Hernández-Julio., Yanagi Jr T., de Fátima  P. M. Á.,Lopes A. M., de Lima R. R . Models for Prediction of Physiological Responses of Holstein Dairy Cows. Applied Artificial Intelligence. 2014;28(8):766-792.
   CrossRef
3. Tyapugin E. A., Tyapugin S. E., Burgomistrova O. N., Khromova O. L.  Breeding cattle on modern complexes with innovative technologies milking. Russian Agricultural Sciences. 2014;6:41-43.
4.  Oskar N., Csilla T., GabrielK. Age-related changes in the concentrations of serum proteins in calves. Journal of Applied Animal Research. 2014;42(4):451-458.
   CrossRef
5. Glagoleva T. I. Ontogenetic dynamics of the main hematological parameters in cattle. Veterinary science, animal science and biotechnology. 2016;5:66-69.
6. Medvedev  I. N., Zavalishina S. Y. Features of the hemostatic system in cows during pregnancy. Russian Agricultural Sciences. 2014;6:44-47.
7. Zavalishina S. Y. State regulation-vascular interactions in newborn piglets with iron with ferroglucin and glikopin. Russian Agricultural Sciences. 2014;1:57-59.
8. Amelina I. V., Medvedev I. N. Transcriptional activity of chromosome nucleolar organizing regions in population of Kursk region. Bulletin of Experimental Biology and Medicine. 2009;147(6):730-732.
   CrossRef
9. Amelina I. V., Medvedev I. N. Evaluation of the dependence of mutagenesis intensity on activity of nucleolus organizer regions of chromosomes in aboriginal population of Kursk region.  Bulletin of Experimental Biology and Medicine. 2008;145(1):68-71.
   CrossRef
10. Medvedev I. N. Platelet functional activity in rats, prolonged experiencing regular exercise. Vestnik SPbSU. Series 3. Biology. 2016;4:99–107. DOI: 10.21638/11701/spbu03.2016.407.
    CrossRef
11. Zavalishina S. Y., Medvedev I. N. Features aggregation erythrocytes and platelets in old rats experiencing regular exercise on a treadmill. Advances in gerontology. 2016;29(3):437-441.
12. Kirilov M. P. The new generation of biologically active substances in the feeding of animals. Feeding of agricultural animals and fodder production. 2006;3:34-37.
13. Medvedev I. N., Savchenko A. P. Platelet activity correction by regular physical training in young people with high normal blood pressure. Russian Journal of Cardiology. 2010;2(82):35-40.
14. Voyevodin., Yu  E.,Ulitk V. E., Lifanov S. P. Morphobiochemical composition of cows blood as criterion of bioactivity of preparation Lipovitam-beta. Zootechniya. 20138:2-3.
15. Simonenko V. B., Medvedev I. N., Kumova T. A. Effect of eprosartan on thrombocytes aggregative capacity in patients with arterial hypertension and metabolic syndrome. Klinicheskaia meditsina. 2008;86(4):19-21.
16. Medvedev I. N., Gromnatskii N. I., Volobuev I. V., Dement’ev V. I., Storozhenko M. V. Thrombocytic hemostasis in hypertensive patients with metabolic syndrome and its correction with lovastatin. Klinicheskaia meditsina. 2004;82(10):37-41.
17. Medvedev I. N., Lapshina E. V., Zavalishina S. Y.  Activity of platelet hemostasis in children with spinal deformities. Bulletin of experimental biology and medicine. 2010;149(5):645-646.
    CrossRef
18. Kutafina N. V., Medvedev I. N. Platelet Aggregation in Clinically Healthy Persons of the Second Coming-of-Age Living in the Kursk Oblast. Advances in Gerontology. 2015;5(4):267-270.
    CrossRef
19. Medvedev I. N., Gromnatskii N. I. Correction of thrombocyte hemostasis and biological age reduction in metabolic syndrome. Klinicheskaia meditsina. 2005;83(8):54-57.
20. Simonenko V. B., Medvedev I. N., Tolmachev V. V. Pathogenetic aspects of arterial hypertension in metabolic syndrome. Klinicheskaia meditsina. 2011;89(1):49-51.
21. Barkagan Z. S., Momot A. P. Diagnosis and therapy controlled hemostasis disorders.  Moscow: N’judiamed. 2008:292.
22. Medvedev I. N., Savchenko A. P., Zavalishina S. Y., Krasnova E. G., Kumova T. A., Gamolina O.V., Skoryatina I. A., Fadeeva T. S. Methodological approaches to the study of the rheological properties of blood in various states. Russian Journal of Cardiology. 2009;5:42-45.
23. Medvedev I. N., Amelina I. V. An association between human morphological phenotypical characteristics and the activity of chromosomal nucleolar organizer regions in the interphase cell nucleus in the population of indigenous people of Kursk region. Morfology. 2012;142(4):87-91.
24. Amelina I. V., Medvedev I. N. Relationship between the chromosome nucleoli-forming regions and somatometric parameters in humans. Bulletin of Experimental Biology and Medicine. 2009; 147(1): 77-80.
    CrossRef
25. Kutafina N. V., Medvedev I. N. Platelet aggregation clinically healthy persons of the second coming of age living in the Kursk region. Advances in gerontology = Uspekhi gerontologii / Rossiĭskai︠a︡ akademii︠a︡ nauk, Gerontologicheskoe obshchestvo. 2015;28(2):321-325.
26. Zavalishina S. Y., Kutafina N. V., Yu A.V., Makurina O. N., Kulikov E. V., Rystsova E. O., Gurina R. R., Sotnikova E. D. Platelet-Activity Dependence on the Age of Rats with Experimental Dyslipidemia. Biol Med (Aligarh). 2016;8:326. doi: 10.4172/0974-8369.1000326.
    CrossRef
27. Medvedev I. N., Gromnatskii N. I., Golikov B. M., Al’-Zuraiki E .M., Li V. I. Effects of lisinopril on platelet aggregation in patients with arterial hypertension with metabolic syndrome. Kardiologiia. 2004;44(10):57-59.
28. Simonenko V. B., Medvedev I. N., Kumova T. A. Pathogenetic aspects of hypertension in case of metabolic syndrome. Voenno–meditsinskii zhurnal. 2010;331(9):41-44.
29. Sizov A. A., Zavalishina S. J. Russian Criminal Legislation in Prevention of Sexually Transmitted Diseases in the Territory of the Russian Federation. Biology and Medicine (Aligarh). 2015;7(5):5.BM-142-15.
30. Medvedev I. N., Skoriatina I. A. Effect of lovastatin on adhesive and aggregation function of platelets in patients with arterial hypertension and dyslipidemia. Klinicheskaia meditsina. 2010;  88(2):38-40.
31. Medvedev I. N., Gromnatskii N. I. The influence of nebivolol on thrombocyte aggregation in patients with arterial hypertension with metabolic syndrome. Klinicheskaia meditsina. 2005; 83(3):31-33.
32. Medvedev I. N., Skoryatina I. A. Fluvastatin effects on blood cell aggregation in patients with arterial hypertension and dyslipidemia. Cardiovascular Therapy and Prevention. 2013;12(2):18-24.
    CrossRef
33. Medvedev I. N., Skoriatina I. A. Dynamics of microrheologic properties of erythrocytes in patients with arterial hypertension and dyslipidemia treated with atorvastatin. Klinicheskaia meditsina, 2012;90(6):42-45.
34. Medvedev, I.N. Dynamics of violations of intravascular platelet activity in  rats during the formation of metabolic syndrome using fructose models. Problems of nutrition, 2016; 85(1) : 42-46.
35. Medvedev, I.N., Savchenko, A.P., Kiperman, Ya.V. Dynamics of the Intravascular Activity of Platelets in Young Men with High Normal Blood Pressure Regularly Practicing Physical Activity. Biology and Medicine (Aligarh), 2015; 7:1 BM-069-15.
36. Simonenko, V.B., Medvedev, I.N., Tolmachev, V.V. Effect of irbesartan on the function of hemocoagulative component of hemostasis in patients with arterial hypertension during metabolic syndrome. Klinicheskaia meditsina, 2010; 88(6) : 27-30.
37. Medvedev, I.N., Zavalishina, S.Yu. Platelet Activity in Patients With Third Degree Arterial Hypertension and Metabolic Syndrome. Kardiologiia, 2016; 56(1) : 48.
    CrossRef
38. Medvedev, I.N., Kumova, T.A. Reduced platelet aggregation in losartan-treated patients with arterial hypertension and metabolic syndrome. Russian Journal of Cardiology, 2008;  1 : 40-42.
39. Gromnatskii, N.I., Medvedev, I.N. Non-pharmacological correction of impaired platelet hemostasis in hypertensive patients with metabolic syndrome. Klinicheskaia meditsina, 2003; 81(4) : 31-34.
40. Medvedev, I.N., Skoryatina, I.A. Erythrocyte aggregation in patients with arterial hypertension and dyslipidemia treated with pravastatin. Klinicheskaia meditsina, 2014; 92(11) : 34-38.
41. Medvedev, I.N., Skoryatina, I.A. Aggregation properties of blood cells and vascular control over them in patients with arterial hypertension and dyslipidemia. Russian Journal of Cardiology, 2015; 4(120) : 18-22.
    CrossRef
42. Medvedev, I.N., Danilenko, O.A. Complex correction of vascular hemostasis in patients with arterial hypertension, metabolic syndrome, and recent ocular vessel occlusion. Russian Journal of Cardiology, 2010; 4(84) : 15-19.
43. Simonenko, V.B., Medvedev, I.N., Mezentseva, N.I., Tolmachev, V.V. The antiaggregation activity of the vascular wall in patients suffering from arterial hypertension with metabolic syndrome. Klinicheskaia meditsina, 2007; 85(7) : 28-30.
44. Medvedev, I.N., Gromnatskii, N.I. The influence of hypocaloric diet on thrombocyte rheology in patients with metabolic syndrome. Klinicheskaia meditsina, 2006; 84(3) : 49-52.
45. Medvedev, I.N., Danilenko, O.A. Effectiveness of vascular wall activity correction in patients with arterial hypertension, metabolic syndrome, and oculo-vascular occlusion. Russian Journal of Cardiology, 2010; 3(83) : 64-67.
46. Simonenko, V.B., Medvedev, I.N., Tolmachev, V.V. Comparative evaluation of the influence of sulfhydryl and phosphate ACE inhibitors on thrombocyte aggregation in patients suffering from arterial hypertension with metabolic syndrome. Klinicheskaia meditsina, 2007; 85(4) : 24-27.
47. Medvedev, I.N., Skoryatina, I.A. Platelet hemostasis dynamics in simvastatin- treated patients with arterial hypertension and dyslipidemia. Russian Journal of Cardiology, 2010; 1(81) : 54-58.
48. Medvedev, I.N., Gromnatskii, N.I. Effect of amlodipine on intravascular thrombocyte activity in patients with arterial hypertension and metabolic syndrome. Klinicheskaia meditsina, 2005; 83(2) : 37-39.
49. Medvedev, I.N., Gromnatskii, N.I., Mokhamed, A.-Z.E. Comparative Assessment of Effects of Qadropril and Enalapril on Tntravascular Activity of Platelets in Hypertensive Patients With Metabolic Syndrome.  Kardiologiia, 2004; 44(12) : 44-46.
50. Simonenko, V.B., Medvedev, I.N., Gamolina, O.V. Primary hemostasis activity in patients with arterial hypertension and impaired glucose tolerance treated with trandolapril. Klinicheskaia meditsina, 2011; 89(2) : 29-31.
51. Simonenko, V.B., Medvedev, I.N., Tolmachev, V.V. Dynamics of primary hemostasis activity in patients with arterial hypertension and metabolic syndrome treated with candesartan. Klinicheskaia meditsina, 2011; 89(3) : 35-38.
52. Simonenko, V.B., Medvedev, I.N., Briukhovetskii, A.G. Effect of therapy with diuretics on the functional activity of platelets in patients with arterial hypertension and abdominal obesity. Klinicheskaia meditsina, 2012; 90(11) : 54-56.
53. Ganguly, K, Murciano, J.C., Westrick, R. The glycocalyx protects erythrocyte-bound tissue-type plasminogen activator from enzymatic inhibition. J. Pharmacol. Exp. Ther, 2007; 321 : 158–164.
    CrossRef
54. Wandersee, N.J., Punzalan, R.C., Rettig, M.P. Erythrocyte adhesion is modified by alterations in cellular tonicity and volume. Brit. J. Haematol, 2005; 3 : 366-377.
    CrossRef
55. Medvedev, I.N., Skoryatina, I.A. Pravastatin in correction of vessel wall antiplatelet control over the blood cells in patients with arterial hypertension and dyslipidemia. Cardiovascular therary and prevention, 2014; 13(6) : 18-22.
    CrossRef
56. Medvedev, I.N., Kumova, T.A., Gamolina, O.V. Renin-angiotensis system role in arterial hypertension development. Russian Journal of Cardiology, 2009; 4 : 82-84.
57. Korepanova, L.V., Starostina, O.S., Batanov, S.D. Blood as an index of interior peculiarities of hybrid animals. Zootechniya, 2015; 10 : 26-28.