eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2017-03-25 10 1 105 110 10.13005/bpj/1087 13649 article Yuriy Nikolaevich Lezhnin 1,2 Dmitry Sergeevich Kravchenko 1,2 Anna Evgenyevna Ivanova 1 Yulia Evgenyevna Kravchenko 1 Elena Ivanovna Frolova 1 Dendritic cells (DC) play a key role in regulation of adaptive immune response by providing effective protection from various infectious agents and pathologies. The main function of dendritic cells is to absorb and present foreign antigens to effector cells of the immune system, such as CD8+ T-lymphocytes. To date, either studying of functions of dendritic cells or their utilization as cellular vaccines in cancer immunotherapy have been performed with various lentivirus-based genetic constructs for silencing or activation of certain genes. Successful infection of dendritic cells is constrained by expression of specific SAM domain and HD domain-containing protein 1 (SAMHD1) by these cells. In this work, we have developed an optimized helper vector for obtaining substantial titers of Vpx-containing lentiviral particles that can efficiently infect human monocytes and dendritic cells. https://biomedpharmajournal.org/vol10no1/construction-of-an-optimized-helper-plasmid-containing-vpx-for-lentiviral-mediated-transduction-of-dendritic-cells/ Dendritic cells lentiviral transduction immunotherapy vpx SAMHD1