{"id":672,"date":"2015-02-15T06:40:59","date_gmt":"2015-02-15T06:40:59","guid":{"rendered":"http:\/\/biomedpharmajournal.org\/?p=672"},"modified":"2020-04-25T07:30:23","modified_gmt":"2020-04-25T07:30:23","slug":"effects-of-bio-inoculants-and-fertilizers-on-growth-of-moringa-oleifera-pkm1-variety","status":"publish","type":"post","link":"https:\/\/biomedpharmajournal.org\/staging\/vol2no1\/effects-of-bio-inoculants-and-fertilizers-on-growth-of-moringa-oleifera-pkm1-variety\/","title":{"rendered":"Effects of Bio-Inoculants and Fertilizers on Growth of Moringa Oleifera PKM1 Variety"},"content":{"rendered":"

Introduction<\/strong><\/p>\n

Moringa oleifera (<\/em>Horseradish tree) <\/em>is the most cultivated species of a monogeneric family, Moringaceae <\/em>native to the Sub–<\/em>Himalayan tracks of India. Recently there is highly demand of some of the hybrid varieties of Moringa<\/em>, especially variety PKM1 (South Indian origin) affecting the growth rate. The present study is to access the effect of different bio-inoculants and fertilizers on the growth and development of Moringa oleifera<\/em> (PKM1) variety under green house condition as a part of rural livelihood support and also for the estimation of different growth parameters like Relative Growth Rate (RGR), Leaf Area Ratio(LAR), Net Assimilation Rate(NAR), \u00a0and Secondary Metabolites(Biomass).<\/p>\n

Materials and Methods <\/strong><\/p>\n

Moringa oleifera PKM1 variety was used as the sample, the experimental set up was done inside the Green house maintaining the temperature 27-280<\/sup>C and water was sprayed inside it. The experimental setup contains 2 trays, each of 48 small pots from the total of 96. The pots were filled with sterilized soil and sand through the entire surface area of each pot. Then the row marked as treatments and columns as replication. There were 12 treatments including control containing 8 replications each. Different fertilizers were added to the respective treatments one by one as per the quantity fixed above. There after water was sprayed & then the seeds sowing inside pot i.e. one seed per pot.<\/p>\n

The day of sowing was count 0 day in observation. After 6 days it started to germinate with minimum height of 1c.m. The observation was taken in 15, 30 & 45 days of germination (Table-2).<\/p>\n

Table 1<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u00a0\u00a0\u00a0\u00a0\u00a0 Treatments\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/strong><\/td>\n\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Fertilizers<\/strong><\/td>\n<\/tr>\n
Control<\/td>\nWithout any fertilizer treatment<\/td>\n<\/tr>\n
T1<\/td>\nUrea (N) Each pot contains 5 ml<\/td>\n<\/tr>\n
T2<\/td>\nS.S.P (P)Each pot contains 5ml<\/td>\n<\/tr>\n
T3<\/td>\nPotash (K) Each pot contains 5ml<\/td>\n<\/tr>\n
T4<\/td>\nShymla 26-26-10 Each pot contains 5ml<\/td>\n<\/tr>\n
T5<\/td>\nBio-organic Annapurna 1gm\/pot<\/td>\n<\/tr>\n
T6<\/td>\nBio-organic Garden samrat 1gm\/pot<\/td>\n<\/tr>\n
T7<\/td>\nBio-organic Biopower 1gm\/pot<\/td>\n<\/tr>\n
T8<\/td>\nFungi 5ml\/pot<\/td>\n<\/tr>\n
T9<\/td>\nFungi 5ml+S.S.P 5ml\/pot<\/td>\n<\/tr>\n
T10<\/td>\nFungi 5ml+S.S.P 5ml+ Annapurna 1gm \/pot<\/td>\n<\/tr>\n
T11<\/td>\nFungi 5ml+ Annapurna 1gm \/pot<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

Table 2 : (H- Height in cm, L- No. of leaves).<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
OBSERVATION<\/strong><\/td>\nAVVRAGE HEIGHT AND NO. OF LEAVES<\/strong><\/td>\n<\/tr>\n
15 DAYS<\/strong><\/td>\n30DAYS<\/strong><\/td>\n45DAYS<\/strong><\/td>\n<\/tr>\n
CONTROL<\/td>\nH<\/td>\n12.35cm<\/td>\n20.44cm<\/td>\n27.97cm<\/td>\n<\/tr>\n
L<\/td>\n28.85<\/td>\n30.71<\/td>\n45.37<\/td>\n<\/tr>\n
T1<\/td>\nH<\/td>\n10.93cm<\/td>\n19.06cm<\/td>\n27.06cm<\/td>\n<\/tr>\n
L<\/td>\n31.66<\/td>\n41.33<\/td>\n54.33<\/td>\n<\/tr>\n
T2<\/td>\nH<\/td>\n15.02cm<\/td>\n24.9cm<\/td>\n36.65cm<\/td>\n<\/tr>\n
L<\/td>\n27<\/td>\n31<\/td>\n38.5<\/td>\n<\/tr>\n
T3<\/td>\nH<\/td>\n9.25cm<\/td>\n21.28cm<\/td>\n30.18cm<\/td>\n<\/tr>\n
L<\/td>\n19.75<\/td>\n25<\/td>\n35.12<\/td>\n<\/tr>\n
T4<\/td>\nH<\/td>\n10.22cm<\/td>\n19.83cm<\/td>\n28.93cm<\/td>\n<\/tr>\n
L<\/td>\n20.8<\/td>\n32.33<\/td>\n43<\/td>\n<\/tr>\n
T5<\/td>\nH<\/td>\n9.41cm<\/td>\n16.64cm<\/td>\n38.8cm<\/td>\n<\/tr>\n
L<\/td>\n26.33<\/td>\n35.28<\/td>\n46.42<\/td>\n<\/tr>\n
T6<\/td>\nH<\/td>\n8.9cm<\/td>\n14.71cm<\/td>\n27.27cm<\/td>\n<\/tr>\n
L<\/td>\n19<\/td>\n28.85<\/td>\n40.28<\/td>\n<\/tr>\n
T7<\/td>\nH<\/td>\n10cm<\/td>\n19.68cm<\/td>\n30.76cm<\/td>\n<\/tr>\n
L<\/td>\n25<\/td>\n34.2<\/td>\n46.8<\/td>\n<\/tr>\n
T8<\/td>\nH<\/td>\n13.88cm<\/td>\n22.78cm<\/td>\n32.65cm<\/td>\n<\/tr>\n
L<\/td>\n23.16<\/td>\n34.16<\/td>\n46.83<\/td>\n<\/tr>\n
T9<\/td>\nH<\/td>\n10.92cm<\/td>\n24.04cm<\/td>\n34.74cm<\/td>\n<\/tr>\n
L<\/td>\n18.4<\/td>\n20.2<\/td>\n39.16<\/td>\n<\/tr>\n
T10<\/td>\nH<\/td>\n10.2cm<\/td>\n19.83cm<\/td>\n30.88cm<\/td>\n<\/tr>\n
L<\/td>\n18<\/td>\n31.25<\/td>\n36.62<\/td>\n<\/tr>\n
T11<\/td>\nH<\/td>\n8.5cm<\/td>\n13.68cm<\/td>\n25.12cm<\/td>\n<\/tr>\n
L<\/td>\n24.85<\/td>\n32.5<\/td>\n44.25<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\u00a0Table 3:<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
treatment<\/strong><\/td>\nRGR(Mg\/gm\/day)<\/strong><\/td>\nNAR(Mg\/cm2\/day)<\/strong><\/td>\nLAR(cm2\/gm)<\/strong><\/td>\n<\/tr>\n
C<\/td>\n0.0013<\/td>\n0.0023<\/td>\n30.885<\/td>\n<\/tr>\n
T1<\/td>\n0.0097<\/td>\n0.0343<\/td>\n30.555<\/td>\n<\/tr>\n
T2<\/td>\n0.0002<\/td>\n0.00017<\/td>\n18.678<\/td>\n<\/tr>\n
T3<\/td>\n0.0019<\/td>\n0.0056<\/td>\n19.929<\/td>\n<\/tr>\n
T4<\/td>\n0.002<\/td>\n0.0049<\/td>\n24.476<\/td>\n<\/tr>\n
T5<\/td>\n0.007<\/td>\n0.0149<\/td>\n22.671<\/td>\n<\/tr>\n
T6<\/td>\n0.0066<\/td>\n0.0116<\/td>\n27.926<\/td>\n<\/tr>\n
T7<\/td>\n0.0085<\/td>\n0.0238<\/td>\n22.429<\/td>\n<\/tr>\n
T8<\/td>\n0.0046<\/td>\n0.0117<\/td>\n24.612<\/td>\n<\/tr>\n
T9<\/td>\n0.0022<\/td>\n0.00061<\/td>\n21.284<\/td>\n<\/tr>\n
T10<\/td>\n0.0046<\/td>\n0.00943<\/td>\n27.373<\/td>\n<\/tr>\n
T11<\/td>\n0.0039<\/td>\n0.00924<\/td>\n26.089<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

(<\/strong>Relative Growth Rate (RGR)<\/strong>, Leaf Area Ratio (LAR),<\/strong> Net Assimilation Rate (NAR)<\/strong>, and Secondary Metabolites (Biomass<\/strong>).)<\/p>\n

\u00a0<\/strong><\/p>\n\n\n\n
\"Graph<\/td>\n\n

Graph 1: (RGR, NAR, LAR & BiomassOF 11 TREATMENTS):<\/strong><\/p>\n

Click here to View Graph<\/a><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n


\nTable 4: Observation Of Biomass Taken At 45 & 60 Days<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Treatments<\/strong><\/td>\nDry biomass<\/strong><\/td>\n<\/tr>\n
At 45 days<\/strong><\/td>\nAt 60 days<\/strong><\/td>\n<\/tr>\n
Control<\/td>\n.505<\/td>\n.532<\/td>\n<\/tr>\n
T1<\/td>\n.333<\/td>\n.609<\/td>\n<\/tr>\n
T2<\/td>\n.765<\/td>\n.805<\/td>\n<\/tr>\n
T3<\/td>\n.674<\/td>\n.688<\/td>\n<\/tr>\n
T4<\/td>\n.662<\/td>\n.712<\/td>\n<\/tr>\n
T5<\/td>\n.412<\/td>\n.567<\/td>\n<\/tr>\n
T6<\/td>\n.520<\/td>\n.519<\/td>\n<\/tr>\n
T7<\/td>\n.402<\/td>\n.568<\/td>\n<\/tr>\n
T8<\/td>\n.444<\/td>\n.533<\/td>\n<\/tr>\n
T9<\/td>\n.502<\/td>\n.762<\/td>\n<\/tr>\n
T10<\/td>\n.580<\/td>\n.592<\/td>\n<\/tr>\n
T11<\/td>\n.489<\/td>\n.538<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/strong><\/p>\n

Result and Discussion<\/strong><\/p>\n

Moringa oleifera<\/em> PKM1 variety shows great response to different fertilizers. As from the above observation it conclude that\u00a0 from the RGR analysis treatment no-1 with urea shows maximum RGR value i.e. 0.0097mg\/gm\/day, NAR value i.e.. 0.0343mg\/cm2\/day & LAR value i.e. 30.555cm2\/gm. Biomass was estimated and was found better in treatment-2\u00a0 in 45 & 60 days with SSP i.e. 0.765gm & 0.805gm respectively. So it concludes that RGR treatment T1 with urea is found beneficial over other application of Bio inoculants and fertilizers. This may be attributed to the uses of more Nitrogen by the plant.<\/p>\n

Reference<\/strong><\/p>\n

    \n
  1. Basak, U.C, AB Das and P. Das (in 1998) seasonal changes in organic constituents in leaves of\u00a0 9th<\/sup> Mangroves species.<\/li>\n
  2. Basak, U.C, AB Das and P. Das (in 1996), chlorophylls carotinoeds proteins and secondary metabolites in leaves of mangroves species of Orissa, bulletin of marine sciences 58 (3) 654-659.<\/li>\n
  3. Lowry, O.H.N.J rosebrough , AL fan & R.J. randall , 1951. protein measurement with the folin phenol reagent, J.boil chem..,193:265-275.<\/li>\n
  4. Dahot\u00a0 MU, SA Ali, et al.(1985) proteolytic enzyme of\u00a0 Moringa oleifera seeds. Journal of pharmacy 6(1-2):1-10.<\/li>\n
  5. Dhar B, and OP Gupta (1982) Nutritional value of Shigru (Moringa oleifera Lam.) B.M.E.B.E.R 3(2-4) 280-288.<\/li>\n
  6. Mishra (1993) Drumstick polysaccharide as pharmaceutical adjuvant. Indian Journal of Natural Products 9(1): 3-6.<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

    Introduction Moringa oleifera (Horseradish tree) is the most cultivated species […]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[6],"tags":[],"class_list":["post-672","post","type-post","status-publish","format-standard","hentry","category-vol2no1"],"_links":{"self":[{"href":"https:\/\/biomedpharmajournal.org\/staging\/wp-json\/wp\/v2\/posts\/672","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/biomedpharmajournal.org\/staging\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biomedpharmajournal.org\/staging\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biomedpharmajournal.org\/staging\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/biomedpharmajournal.org\/staging\/wp-json\/wp\/v2\/comments?post=672"}],"version-history":[{"count":5,"href":"https:\/\/biomedpharmajournal.org\/staging\/wp-json\/wp\/v2\/posts\/672\/revisions"}],"predecessor-version":[{"id":32983,"href":"https:\/\/biomedpharmajournal.org\/staging\/wp-json\/wp\/v2\/posts\/672\/revisions\/32983"}],"wp:attachment":[{"href":"https:\/\/biomedpharmajournal.org\/staging\/wp-json\/wp\/v2\/media?parent=672"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biomedpharmajournal.org\/staging\/wp-json\/wp\/v2\/categories?post=672"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biomedpharmajournal.org\/staging\/wp-json\/wp\/v2\/tags?post=672"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}