{"id":56940,"date":"2024-03-20T11:22:04","date_gmt":"2024-03-20T11:22:04","guid":{"rendered":"https:\/\/biomedpharmajournal.org\/?p=56940"},"modified":"2024-04-03T05:06:35","modified_gmt":"2024-04-03T05:06:35","slug":"development-of-rice-by-products-based-hair-tonic-mixed-with-traditional-thai-herbal-extracts-a-sustainable-approach-for-hair-care","status":"publish","type":"post","link":"https:\/\/biomedpharmajournal.org\/staging\/vol17no1\/development-of-rice-by-products-based-hair-tonic-mixed-with-traditional-thai-herbal-extracts-a-sustainable-approach-for-hair-care\/","title":{"rendered":"Development of Rice By-Products Based Hair Tonic Mixed with Traditional Thai Herbal Extracts: A Sustainable Approach for Hair Care"},"content":{"rendered":"\n

Introduction<\/strong><\/p>\n\n\n\n

The market for hair loss prevention products had a value of US$ 23,599.87 million in 2021. It is projected to increase at a compound annual growth rate (CAGR) of 4.2% from 2021 to 2028, reaching US$ 31,524.52 million by 2028.1<\/sup> This tends to expand as the population increases. In Thailand, hair loss prevention products are products that come from both domestic production bases and imported from abroad which can be divided into 2 groups: medicines and cosmetics. There are only two approved drugs, namely Minoxidil and Finasteride, that can only be used to treat people with hereditary hair loss problems, but the disadvantage is that they cause unwanted side effects.2<\/sup> In addition, premature graying of hair (premature canities) is considered a health problem that has a negative effect on mental health and personality.3<\/sup> The cause of it may be due to genetics, aging, reduction in the number of melanocytes responsible for producing the pigment melanin, deficiency in tyrosinase enzyme activity responsible for converting the precursor tyrosine into melanin at the hair roots, oxidative stress resulting in the body creating the free radicals such as hydrogen peroxide in large quantities and causes melanocyte cells to decrease and die quickly (apoptosis).4<\/sup>      <\/p>\n\n\n\n

Dandruff is a scalp condition that primarily affects males. Topical agents and synthetic drugs utilized in the treatment of dandruff exhibit distinct adverse effects, such as localized burning sensation, depressive symptoms, dizziness, headaches, pruritus or dermatitis, nausea, abdominal discomfort, alterations in vision, vomiting, hair discoloration, scalp dryness or oiliness, and heightened hair loss.5<\/sup> Hence, the utilization of plant extracts holds significant potential in both the management and prophylaxis of dandruff.<\/p>\n\n\n\n

Prior research demonstrated that broken riceberry (BRB) and crude riceberry oil (CRO), byproducts of the Thai rice mill industry, had the potential to stimulate growth of hair and hair melanogenesis. It is known that CRO and BRB contained \u03b3-oryzanol and fatty acids (oleic acid, linoleic acid, stearic acid). 6<\/sup> These compounds were proved to have potential as a treatment for hair loss.7<\/sup> The antioxidant activity of the BRB extract was determined to be 6.36 mg Trolox Equivalent Antioxidant Capacity (TEAC)\/g (DPPH assay), 1.33 mg FeSO4<\/sub>\/g (FRAP assay), and 5.82 mg TEAC\/g (ABTS assay), with respective TPC and TFC values of 9.94 mg gallic acid equivalent (GAE)\/g and 67.2 mg catechin equivalent (CE)\/g, respectively. It is noteworthy that the ethanolic CRO and aqueous BRB extract demonstrated tyrosinase-stimulating activities of 26% and 45%, respectively.6<\/sup> Additionally, it has been reported that some Thai traditional herbs can stimulate cells in the hair roots causing new hair to be created and reducing hair loss. Acacia concinna<\/em> (AC) in the Fabaceae family whose pods contain 20.8% saponin, including acacinin A, B, C, D and E. It acts as a surfactant, foaming agent, hair conditioner, hair volumizer, hair fortifier, hair stimulant with antibacterial, and anti-dandruff properties.8,9<\/sup> Catunaregam tomentosa<\/em> (CT) in the Rubiaceae family contains tannins and cardiac glycosides. The total amount of phenolic content from methanolic extract of fruits was 423.70+3.73 mg GAE\/g extract with DPPH antioxidant activity (IC50<\/sub> = 5.12+0.34 mg\/mL).10<\/sup> Its ripe fruits are used for hair washing. 11 <\/sup>Tinospora crispa<\/em> (TC) in the Menispermaceae family contains alkaloids, flavonoids, flavone glycosides, triterpenes, which had high antioxidant activity.12,13<\/sup> The extract has a FRAP value of 1,1011.11 \u00b1 1145.42 \u03bcmol Fe+2<\/sup>\/g and a DPPH inhibition of 55.79 \u00b1 7.90%, with an IC50 <\/sub>of 22 \u03bcg\/mL. 14<\/sup> In this context, CRO, BRB, CT, AC, and TC emerge as promising candidates, each known for its unique phytochemical composition and traditional applications. Hair care has long been a focal point in the pursuit of well-being. The quest for effective and natural solutions has led researchers to explore the potential of natural or botanical extracts in developing innovative hair tonics. This research aimed to develop five hair tonic formulae by mixing CRO (0.2-1.0%) and BRB (0.4-2.0%) with three Thai traditional herbal ethanolic extracts (2.0%) of CT, AC and TC in 4:3:3 (v\/v) and tested them on twenty subjects for anti-hair loss, anti-grey hair and anti-dandruff evaluation of the products. This could pave the way for advancements in the field of hair tonic development and reinforce the significance of nature-inspired solutions in contemporary cosmetology.<\/p>\n\n\n\n

Materials and Methods<\/strong><\/p>\n\n\n\n

Agricultural Materials<\/strong><\/p>\n\n\n\n

Organic BRB and CRO were obtained from the E-san Community Enterprise\nAgrarian Network, Roi Et Province, Thailand.6<\/sup> Fruits of CT, pods of AC, and stems of TC were\ncollected in September 2018 (rainy season) from\nKu Thong subdistrict, Chiang Yuen District, Maha Sarakham Province, Thailand.<\/p>\n\n\n\n

Extraction\nof BRB, CRO and Thai Local Herbs<\/strong><\/p>\n\n\n\n

Extraction\nof BRB and CRO was conducted as a previous report.6<\/sup>\nEach herb was extracted using maceration method 14<\/sup> by taking\n200 g of dried fruits (CT), pods (AC), stems (TC) and extracting them with 1 L of ethanol for 3 days in\na shaker at 200 rpm at 40 \u00b0C. Ethanol from the extract was evaporated using a\nrotary evaporator at 42 \u00b0C. The herbal extract was dissolved in distilled water\nto make a concentration of 20 mg\/mL crude plant extract and stored at -20 \u00b0C.<\/p>\n\n\n\n

Hair Tonic Formulations<\/strong><\/p>\n\n\n\n

Five formulae (F1-F5)(Figure 1) of hair tonic products were shown in Table 1. The preparation of each formula is as follows: (1) Dissolve EDTA and add it to ddH2<\/sub>O. (2) Add propylene glycol to Step 1and mix well. (3) Add PEG40 Hydrogenated castor oil to Step 2 <\/strong>and mix well. (4) Add BRB extract and herbal extracts into Step 3 and mix well. (5) Add CRO along with Emulgen (emulsifier). The concentration of BRB in a range of 0.4\u20132.0%, CRO at 0.2\u20131.0% and herbal extracts at 2.0% were implemented based on a range of concentrations used in the previous studies that formulated effective hair tonics for with bioactive oil ingredient at 0.1\u20130.9% 15<\/sup> or rice bran extract at 0.5% for hair growth in human subjects. 7<\/sup> (6) Make a homogeneous emulsion using an ultrasonic bath for 15 min at room temperature (37 \u00b0C in Thailand). (7) Adjust pH using Triethanolamine to 5.5. (8) Filter the mixture through Whatman filter paper no. 1 (0.45 \u00b5m), then filter the clear part through a membrane filter (0.2 \u00b5m). (9) Package in a 200 mL plastic bottle pre-sterilized by UV for 45 min, and (10) Store at room temperature. The chemical compositions e.g. propylene glycol, emulgen, PEG40 hydrogenated castor oil, glycerin, tea tree essential oil, rosemary essential oil, rice milk essential oil, phenoxyethanol and coffee extract in Table 1 were purchased from a chemical store (www.chemipan.com). After that, the above five formulae of hair tonics were tested for their stimulating effect on melanin synthesis using tyrosinase activity assay and for their antioxidant activities. The formula with the highest antioxidant activity and the highest tyrosinase stimulating activity was chosen to test with subjects next.<\/p>\n\n\n\n\n\n
\"\"<\/td>\nFigure 1: Hair tonic products formulae 1-5 in a volume of 20 mL<\/strong>\n

\u00a0<\/p>\n

Click here to view Figure<\/a><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/p>\n\n\n

Table 1: Compositions for 5 formulae (F1-F5) of hair tonic products<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\n

Composition (mL)<\/strong><\/p>\n<\/td>\n

\n

F1<\/strong><\/p>\n<\/td>\n

\n

F2<\/strong><\/p>\n<\/td>\n

\n

F3<\/strong><\/p>\n<\/td>\n

\n

F4<\/strong><\/p>\n<\/td>\n

\n

F5<\/strong><\/p>\n<\/td>\n<\/tr>\n

\n

BRB extract 20 mg\/mL (aq)<\/p>\n<\/td>\n

\n

2.0 (0.4%)<\/p>\n<\/td>\n

\n

4.0 (0.8%)   <\/p>\n<\/td>\n

\n

6.0 (1.2%)   <\/p>\n<\/td>\n

\n

8.0 (1.6%)  <\/p>\n<\/td>\n

\n

10.0 (2.0%)<\/p>\n<\/td>\n<\/tr>\n

\n

Herbal extracts 20 mg\/mL (aq) (CT:AC:TC in 4:3:3) <\/p>\n<\/td>\n

\n

10.0 (2.0%)  <\/p>\n<\/td>\n

\n

10.0 (2.0%)  <\/p>\n<\/td>\n

\n

10.0 (2.0%)  <\/p>\n<\/td>\n

\n

10.0 (2.0%)  <\/p>\n<\/td>\n

\n

10.0 (2.0%)  <\/p>\n<\/td>\n<\/tr>\n

\n

CRO 20 mg\/mL (aq)<\/p>\n<\/td>\n

\n

1.0 (0.2%)  <\/p>\n<\/td>\n

\n

2.0 (0.4%)   <\/p>\n<\/td>\n

\n

3.0 (0.6%)   <\/p>\n<\/td>\n

\n

4.0 (0.8%)  <\/p>\n<\/td>\n

\n

5.0(1.0%)<\/p>\n<\/td>\n<\/tr>\n

\n

Propylene glycol<\/p>\n<\/td>\n

\n

2.0              <\/p>\n<\/td>\n

\n

2.0              <\/p>\n<\/td>\n

\n

2.0              <\/p>\n<\/td>\n

\n

2.0              <\/p>\n<\/td>\n

\n

2.0              <\/p>\n<\/td>\n<\/tr>\n

\n

Emulgen<\/p>\n<\/td>\n

\n

2.0              <\/p>\n<\/td>\n

\n

2.0              <\/p>\n<\/td>\n

\n

2.0              <\/p>\n<\/td>\n

\n

2.0              <\/p>\n<\/td>\n

\n

2.0              <\/p>\n<\/td>\n<\/tr>\n

\n

PEG40 Hydrogenated castor oil  <\/p>\n<\/td>\n

\n

0.3              <\/p>\n<\/td>\n

\n

0.3              <\/p>\n<\/td>\n

\n

0.3              <\/p>\n<\/td>\n

\n

0.3              <\/p>\n<\/td>\n

\n

0.3              <\/p>\n<\/td>\n<\/tr>\n

\n

Glycerin<\/p>\n<\/td>\n

\n

3.0              <\/p>\n<\/td>\n

\n

3.0              <\/p>\n<\/td>\n

\n

3.0              <\/p>\n<\/td>\n

\n

3.0              <\/p>\n<\/td>\n

\n

3.0              <\/p>\n<\/td>\n<\/tr>\n

\n

Tea tree essential oil<\/p>\n<\/td>\n

\n

1.0              <\/p>\n<\/td>\n

\n

1.0              <\/p>\n<\/td>\n

\n

1.0              <\/p>\n<\/td>\n

\n

1.0              <\/p>\n<\/td>\n

\n

1.0              <\/p>\n<\/td>\n<\/tr>\n

\n

Rosemary essential oil<\/p>\n<\/td>\n

\n

1.0              <\/p>\n<\/td>\n

\n

1.0              <\/p>\n<\/td>\n

\n

1.0              <\/p>\n<\/td>\n

\n

1.0              <\/p>\n<\/td>\n

\n

1.0              <\/p>\n<\/td>\n<\/tr>\n

\n

Rice milk essential oil<\/p>\n<\/td>\n

\n

1.0              <\/p>\n<\/td>\n

\n

1.0              <\/p>\n<\/td>\n

\n

1.0              <\/p>\n<\/td>\n

\n

1.0              <\/p>\n<\/td>\n

\n

1.0              <\/p>\n<\/td>\n<\/tr>\n

\n

Phenoxyethanol (0.1% v\/v)           <\/p>\n<\/td>\n

\n

0.1             <\/p>\n<\/td>\n

\n

0.1             <\/p>\n<\/td>\n

\n

0.1             <\/p>\n<\/td>\n

\n

0.1             <\/p>\n<\/td>\n

\n

0.1             <\/p>\n<\/td>\n<\/tr>\n

\n

Coffee extract                               <\/p>\n<\/td>\n

\n

0.1             <\/p>\n<\/td>\n

\n

0.1             <\/p>\n<\/td>\n

\n

0.1             <\/p>\n<\/td>\n

\n

0.1             <\/p>\n<\/td>\n

\n

0.1             <\/p>\n<\/td>\n<\/tr>\n

\n

ddH2<\/sub>O<\/p>\n<\/td>\n

\n

adjust the volume to 100 mL<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

aq = aqueous<\/p>\n\n\n

Antioxidant Activities of Hair Tonic Formulae<\/strong><\/p>\n\n\n\n

Scavenging of\n2,2-Diphenyl-1- Picrylhydrazyl (DPPH) free radical scavenging assay, ferric\nreducing antioxidant power assay (FRAP), 2,2\u2019-azino-di(3-Ethyl-benzthiazoline\nsulfonic (ABTS)\u2022+ scavenging assay were conducted\nas per a previous work. 6<\/sup><\/p>\n\n\n\n

Tyrosinase Stimulating\nActivity<\/strong> of Hair Tonic Formulae<\/strong><\/p>\n\n\n\n

This experiment was conducted as per a previous work. 6<\/sup><\/p>\n\n\n\n

Hair Tonic Stability Test<\/strong><\/p>\n\n\n\n

The stability after preparing the hair tonic products was determined by\nobserving the following properties: (1) Color, observed with eyes. (2) Fragrance,\nobserved by smelling. (3) Viscosity (cP) measured by a viscometer. (4)\nSegregation, observed by centrifugation at 5,000 rpm for 30 min. (5) pH measured\nby a pH meter. The product stability was tested using alternating hot and cold\ntemperature conditions (heating\/cooling) for 6 cycles, alternating at 4 \u00b0C and\n45 \u00b0C every 24 h, and the above five properties were observed. The product\nstability was also tested upon storage of the products at room temperature for\n8 weeks, and the above properties were observed.<\/p>\n\n\n\n

Irritation Test Using a Single Patch Test<\/strong><\/p>\n\n\n\n

One mL of hair tonic was pipetted onto a patch test strip using a 0.5\ninchwide gauze cloth. The skin was wiped clean with 70% ethanol. The prepared\ntest strip was placed on the front of the volunteer’s arm and covered it with\ngauze tape to prevent the gauze from slipping and left for 48 h. Afterwards,\nthe test strip was peeled off and the symptoms of irritation on the skin were\nscored on the level of 0-5 (Table 2) within 30 min.<\/p>\n\n\n\n

Table 2: Irritation scores <\/strong><\/p>\n\n\n\n\n\n\n
\n

Symptoms of irritation<\/p>\n<\/td>\n

\n

Score<\/p>\n<\/td>\n<\/tr>\n

\n

No swelling or redness<\/p>\n

Slightly red<\/p>\n

Very red, but the skin on the arm is still smooth<\/p>\n

Red and bumps appear<\/p>\n

Red, blistered, swollen, congested with blood<\/p>\n<\/td>\n

\n

0<\/p>\n

1<\/p>\n

2<\/p>\n

3<\/p>\n

4<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n

Efficacy of Reducing Hair Loss, Gray Hair and Dandruff Reduction of Hair\nTonic on Subjects<\/strong><\/p>\n\n\n\n

The efficacy study mentioned below comprised 20 healthy Thai subjects,\naged between 20 and 60, who were experiencing dandruff, premature canities, or\nhair loss (Table 3). <\/p>\n\n\n\n

Prior to enrollment, all recruited\nparticipants were provided with comprehensive information about the study by\nwritten and verbal means. They subsequently signed a written consent form,\nwhich had been authorized by the Ethical Council of Mahasarakham University,\nThailand (Approval no. 018\/2560). The study involving human participants adhered to the latest\nDeclaration of Helsinki in all aspects.<\/p>\n\n\n\n

The experiment was conducted according\nto the method of Chaisripipat et al. (2015)17<\/sup> using a total of 20 subjects. A randomized, double-blind,\nplacebo-controlled experiment with a split-head method was used to compare\ntreatments in the same individuals (intra-individual trial) divided into 2\nexperimental groups: Group 1: 10 subjects tested a control hair tonic (C) (not\nmixed with rice byproducts or herbs)(Figure 2A) on the left side of the scalp and\nat the same time tested F5 hair tonic (R)(Figure 2B) on the right side of the\nscalp. Group 2: Another 10 subjects tested the commercial hair tonic Giffarine\nHerbita (G) (Figure 2C) on the left side of the scalp and at the same time,\ntested F5 hair tonic (R) on the right side of the scalp. The subjects were\nrandomly assigned to apply 5 drops (0.13 g) of hair tonic on one side of their\nhead and equal amounts of another hair tonic formulation on the other. The\nchemical was applied to the scalp with the same hand and head location\nthroughout the research. The control cleansing shampoo, No More Tears (Johnson\n& Johnson, Thailand), was used to wash hair every two days at 10 g each\nwash.<\/p>\n\n\n\n

To test the efficacy of reducing hair\nloss, each subject used a comb to detangle each side of their hair. The hair\nthat fell from each side of the scalp (was then collected on white paper in the\nmorning and evening on days 0, 10, 20, and 30. The amount of hair lost was\nrecorded to find the average % reduction in hair loss on days 0, 10, 20, and 30\nafter using hair tonics.<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

Table 3: General information of 20 subjects in 3 groups<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
\n

Age (years)<\/p>\n<\/td>\n

\n

No. of subjects<\/p>\n<\/td>\n

\n

%<\/p>\n<\/td>\n<\/tr>\n

\n

18-30<\/p>\n<\/td>\n

\n

7<\/p>\n<\/td>\n

\n

35<\/p>\n<\/td>\n<\/tr>\n

\n

31-40<\/p>\n<\/td>\n

\n

4<\/p>\n<\/td>\n

\n

20<\/p>\n<\/td>\n<\/tr>\n

\n

41-50<\/p>\n<\/td>\n

\n

4<\/p>\n<\/td>\n

\n

20<\/p>\n<\/td>\n<\/tr>\n

\n

51-60<\/p>\n<\/td>\n

\n

5<\/p>\n<\/td>\n

\n

25<\/p>\n<\/td>\n<\/tr>\n

\n

Gender<\/p>\n<\/td>\n

\n

No. of subjects<\/p>\n<\/td>\n

\n

%<\/p>\n<\/td>\n<\/tr>\n

\n

Male<\/p>\n<\/td>\n

\n

2<\/p>\n<\/td>\n

\n

10<\/p>\n<\/td>\n<\/tr>\n

\n

Female<\/p>\n<\/td>\n

\n

18<\/p>\n<\/td>\n

\n

90<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n

Group 1: Use of C and R hair tonics; n<\/em> = 10<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
\n

Age (years)<\/p>\n<\/td>\n

\n

No. of subjects<\/p>\n<\/td>\n

\n

%<\/p>\n<\/td>\n<\/tr>\n

\n

18-30<\/p>\n<\/td>\n

\n

0<\/p>\n<\/td>\n

\n

0<\/p>\n<\/td>\n<\/tr>\n

\n

31-40<\/p>\n<\/td>\n

\n

3<\/p>\n<\/td>\n

\n

30<\/p>\n<\/td>\n<\/tr>\n

\n

41-50<\/p>\n<\/td>\n

\n

2<\/p>\n<\/td>\n

\n

20<\/p>\n<\/td>\n<\/tr>\n

\n

51-60<\/p>\n<\/td>\n

\n

5<\/p>\n<\/td>\n

\n

50<\/p>\n<\/td>\n<\/tr>\n

\n

Gender<\/p>\n<\/td>\n

\n

No. of subjects<\/p>\n<\/td>\n

\n

%<\/p>\n<\/td>\n<\/tr>\n

\n

Male<\/p>\n<\/td>\n

\n

1<\/p>\n<\/td>\n

\n

10<\/p>\n<\/td>\n<\/tr>\n

\n

Female<\/p>\n<\/td>\n

\n

9<\/p>\n<\/td>\n

\n

90<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n

Group 2: Use of G and R hair tonics; n<\/em> = 10<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
\n

Age (years)<\/strong><\/p>\n<\/td>\n

\n

No. of subjects<\/strong><\/p>\n<\/td>\n

\n

%<\/strong><\/p>\n<\/td>\n<\/tr>\n

\n

18-30<\/p>\n<\/td>\n

\n

7<\/p>\n<\/td>\n

\n

70<\/p>\n<\/td>\n<\/tr>\n

\n

31-40<\/p>\n<\/td>\n

\n

1<\/p>\n<\/td>\n

\n

10<\/p>\n<\/td>\n<\/tr>\n

\n

41-50<\/p>\n<\/td>\n

\n

2<\/p>\n<\/td>\n

\n

20<\/p>\n<\/td>\n<\/tr>\n

\n

51-60<\/p>\n<\/td>\n

\n

0<\/p>\n<\/td>\n

\n

0<\/p>\n<\/td>\n<\/tr>\n

\n

Gender<\/p>\n<\/td>\n

\n

No. of subjects<\/p>\n<\/td>\n

\n

%<\/p>\n<\/td>\n<\/tr>\n

\n

Male<\/p>\n<\/td>\n

\n

1<\/p>\n<\/td>\n

\n

10<\/p>\n<\/td>\n<\/tr>\n

\n

Female<\/p>\n<\/td>\n

\n

9<\/p>\n<\/td>\n

\n

90<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n
\"\"<\/td>\n\n

Figure 2:<\/strong> Hair tonic products in 200 mL were used in testing with subjects
(A) Control hair tonic, coded C (without BRB, CRO or herbal extracts). (B) F5 hair tonic, coded R. <\/strong><\/p>\n

<\/p>\n

Click here to view Figure<\/a><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n

To test the efficacy of preventing gray\nhair, each volunteer’s gray hair was counted from 4 head areas (an area of \u200b\u200b1\ncm2<\/sup>) on each side of their head on days 0, 10, 20, and 30 after\nusing the hair tonic. 18<\/sup> The number of gray hair in each of the 4 areas on each side of the head\nadded together was recorded to find the average value of gray hair on each\nside. <\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

To evaluate the anti-dandruff treatment,\na sticky disc was used to lift the dandruff on the hair of each volunteer on\ndays 0, 10, 20, and 30. Chaisripipat et al. (2015) scores were recorded on\neither side of the head in triplicate. R = (Dt<\/sub>-D0<\/sub>)\/D0<\/sub>\u00d7100,\nwhere R reflects decreased efficacy, Dt<\/sub> represents the dandruff\nscale at days 10, 20, and 30, and D0<\/sub> represents the dandruff scale\nat day 0.<\/p>\n\n\n\n

Satisfaction\n<\/strong>Survey<\/strong><\/p>\n\n\n\n

All subjects took a satisfaction survey using the interview\nquestionnaire on a 5-point likeness scale (1 = significant dislike; 5 =\ngreatest likeness). <\/p>\n\n\n\n

Statistical Analysis<\/strong><\/p>\n\n\n\n

The measurements in triplicate were expressed as means \u00b1 standard deviation (SD). Significant\ndifferences were determined by one-way analysis of variance (ANOVA) and\nDuncan\u2019s multiple range test at p<\/em> < 0.05 using the SPSS package (demo\nversion). <\/p>\n\n\n\n

Results and Discussion<\/strong><\/p>\n\n\n\n

Antioxidant Activity and Tyrosinase Stimulation\nof the Hair Tonic Formulae<\/strong><\/strong><\/p>\n\n\n\n

The\nresults showed that F5, amongst hair tonic formulae 1-5, gave the highest\nantioxidant activity of all 3 methods, including DPPH scavenging capacity at\n16.6 TEAC mg\/g, FRAP value at 1.18 FeSO4<\/sub> mg\/g, and ABTS value at\n14.9 TEAC mg\/g (Figure 3A-C). This is probably due to the highest ingredients from BRB\nextract at 2% and agricultural by-products from CRO at 2%. The stimulation of\nthe tyrosinase enzyme is also the highest at 38% tyrosinase stimulation for F5\nproduct (Figure 3D). It was discovered that gamma-oryzanol can induce hair\nfollicle formation, stimulate melanogenesis, and promote hair growth 19<\/sup>; therefore, our BRB and CRO extracts\ncontaining gamma-oryzanol and fatty acids have the potential to be incorporated\ninto hair treatment products.6<\/sup> Antioxidants from herbal extracts in\nhair function by safeguarding the hair keratin, which is responsible for\ncausing damage to the hair structure; preventing the degradation of integral\nlipids in the hair fiber, which weakens the cell membranes and leads to hair\nbrittleness and loss; preventing the oxidation of melanin, which is responsible\nfor hair whitening; and repairing and protecting damage by counteracting free\nradicals and decelerating lipid oxidation. 19<\/sup><\/p>\n\n\n\n

Hair Tonic Product Stability Test<\/strong><\/p>\n\n\n\n

When stored at room temperature for 8 weeks, F5 hair tonic product was found to be stable, did not separate into layers, had no viscosity (< 5 cP), did not change color, had constant pH of 5.5 and had no reduction in the fragrance of rice milk (Table 4). However, when testing the stability of the hair tonic formula where low temperature conditions alternated with high temperature (at 4 \u00b0C 24 h alternating with 45 \u00b0C 24 h) for 6 cycles, it was found that the fragrance of the hair tonic product decreased. When the experiment was carried out to the 9th<\/sup> – 12th<\/sup> day, no separation of layers, no viscosity, and no color change (Table 4) indicating that the F5 hair tonic formula was stable upon storage at room temperature.<\/p>\n\n\n\n

Regarding hair tonic lotion preparations intended for application on the scalp, SNI standard number 16-4955-1998 specifies that the pH of hair tonic preparations must fall within the range of 3.0 to 7.0. 20<\/sup> Excessive acidity can lead to skin irritation, while excessive alkalinity can result in parched, scaly skin. It can be inferred that the pH value of this formula is satisfactory. In addition, SNI indicates that a good hair tonic has a viscosity of <5 cPs 20<\/sup> which was following our results (Table 4).<\/p>\n\n\n\n\n\n
\"\"<\/td>\n\n

Figure 3:<\/strong> Antioxidant activity of 5 hair tonic formulae (1-5) and tyrosinase stimulating property (A) DPPH scavenging activity assay. (B) FRAP assay.<\/strong><\/p>\n

<\/p>\n

Click here to view Figure<\/a><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n

Table 4: Stability test of F5 hair tonic with alternating low temperature and high temperature conditions for 6 cycles.<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\n

Temp. (\u00b0C)<\/strong><\/p>\n<\/td>\n

\n

Day<\/strong><\/p>\n<\/td>\n

\n

Color<\/strong><\/p>\n<\/td>\n

\n

Fragrance<\/strong><\/p>\n<\/td>\n

\n

Separation<\/strong><\/p>\n<\/td>\n

\n

Viscosity (cP)<\/strong><\/p>\n<\/td>\n

\n

pH<\/strong><\/p>\n<\/td>\n<\/tr>\n

\n

4<\/p>\n<\/td>\n

\n

1<\/p>\n<\/td>\n

\n

Light yellow<\/p>\n<\/td>\n

\n

Strong<\/p>\n<\/td>\n

\n

No<\/p>\n<\/td>\n

\n

< 5<\/p>\n<\/td>\n

\n

5.5<\/p>\n<\/td>\n<\/tr>\n

\n

45<\/p>\n<\/td>\n

\n

2<\/p>\n<\/td>\n

\n

Light yellow<\/p>\n<\/td>\n

\n

Strong<\/p>\n<\/td>\n

\n

No<\/p>\n<\/td>\n

\n

< 5<\/p>\n<\/td>\n

\n

5.5<\/p>\n<\/td>\n<\/tr>\n

\n

4<\/p>\n<\/td>\n

\n

3<\/p>\n<\/td>\n

\n

Light yellow<\/p>\n<\/td>\n

\n

Strong<\/p>\n<\/td>\n

\n

No<\/p>\n<\/td>\n

\n

< 5<\/p>\n<\/td>\n

\n

5.5<\/p>\n<\/td>\n<\/tr>\n

\n

45<\/p>\n<\/td>\n

\n

4<\/p>\n<\/td>\n

\n

Light yellow<\/p>\n<\/td>\n

\n

Strong<\/p>\n<\/td>\n

\n

No<\/p>\n<\/td>\n

\n

< 5<\/p>\n<\/td>\n

\n

5.5<\/p>\n<\/td>\n<\/tr>\n

\n

4<\/p>\n<\/td>\n

\n

5<\/p>\n<\/td>\n

\n

Light yellow<\/p>\n<\/td>\n

\n

Strong<\/p>\n<\/td>\n

\n

No<\/p>\n<\/td>\n

\n

< 5<\/p>\n<\/td>\n

\n

5.5<\/p>\n<\/td>\n<\/tr>\n

\n

45<\/p>\n<\/td>\n

\n

6<\/p>\n<\/td>\n

\n

Light yellow<\/p>\n<\/td>\n

\n

Strong<\/p>\n<\/td>\n

\n

No<\/p>\n<\/td>\n

\n

< 5<\/p>\n<\/td>\n

\n

5.5<\/p>\n<\/td>\n<\/tr>\n

\n

4<\/p>\n<\/td>\n

\n

7<\/p>\n<\/td>\n

\n

Light yellow<\/p>\n<\/td>\n

\n

Strong<\/p>\n<\/td>\n

\n

No<\/p>\n<\/td>\n

\n

< 5<\/p>\n<\/td>\n

\n

5.5<\/p>\n<\/td>\n<\/tr>\n

\n

45<\/p>\n<\/td>\n

\n

8<\/p>\n<\/td>\n

\n

Light yellow<\/p>\n<\/td>\n

\n

Strong<\/p>\n<\/td>\n

\n

No<\/p>\n<\/td>\n

\n

< 5<\/p>\n<\/td>\n

\n

5.5<\/p>\n<\/td>\n<\/tr>\n

\n

4<\/p>\n<\/td>\n

\n

9<\/p>\n<\/td>\n

\n

Light yellow<\/p>\n<\/td>\n

\n

Moderate<\/p>\n<\/td>\n

\n

No<\/p>\n<\/td>\n

\n

< 5<\/p>\n<\/td>\n

\n

5.5<\/p>\n<\/td>\n<\/tr>\n

\n

45<\/p>\n<\/td>\n

\n

10<\/p>\n<\/td>\n

\n

Light yellow<\/p>\n<\/td>\n

\n

Moderate<\/p>\n<\/td>\n

\n

No<\/p>\n<\/td>\n

\n

< 5<\/p>\n<\/td>\n

\n

5.5<\/p>\n<\/td>\n<\/tr>\n

\n

4<\/p>\n<\/td>\n

\n

11<\/p>\n<\/td>\n

\n

Light yellow<\/p>\n<\/td>\n

\n

Light<\/p>\n<\/td>\n

\n

No<\/p>\n<\/td>\n

\n

< 5<\/p>\n<\/td>\n

\n

5.5<\/p>\n<\/td>\n<\/tr>\n

\n

45<\/p>\n<\/td>\n

\n

12<\/p>\n<\/td>\n

\n

Light yellow<\/p>\n<\/td>\n

\n

Light<\/p>\n<\/td>\n

\n

No<\/p>\n<\/td>\n

\n

< 5<\/p>\n<\/td>\n

\n

5.5<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n

Efficacy in Reducing Hair Loss, Gray Hair and Dandruff of Hair Tonic on Subjects<\/strong><\/p>\n\n\n\n

Adverse reactions i.e. irritations were not noticed during the study. The results of hair loss reduction showed that in group 1 with the average age of adulthood and elderhood, a reduction in hair loss from using the control (C) and experimental (R) hair tonic product was found in the range of 64-78% and 60-71%, respectively, over 30 days with no significance between C and R, and with increasing duration time (Table 5). Group 2 (a younger age range) found that the reduction in hair loss from using commercial hair tonic product (G) and experimental (R) hair tonic products were in the range of 31-67% and 20-48%, respectively over 30 days with no significance between G and R (Table 5). However, the increasing efficacy in the reduction of hair loss was observed from day 10 to 30 in these 2 products. In comparison with the efficacy in the reduction of hair loss of the 2 groups at each interval (10, 20 and 30 days), the experimental (R) hair tonic product was found to be more effective in group 1 (older age) than group 2 (younger age).<\/p>\n\n\n\n

As for the test results of the efficacy of hair tonic in reducing gray hair, it was found that in group 1, no impact was noticed. The control set (C) and the experimental set (R) gave the reduction of gray hair in the range of 0.5-14.3% and 0.5-20%, respectively, over a period of 30 days with no significance between C and R (Table 5). However, the increasing efficacy in reduction of gray hair was observed from day 10 to 30 in these 2 products indicating that if used continuously, it would reduce gray hair better. In group 2, it was found that the reduction of gray hair was more effective. The use of commercial hair tonic product (G) and trial set (R) was found in the range of 7-26% and 9-27% reduction of gray hair, respectively over a period of 30 days with no significance between G and R (Table 5). In comparison with the efficacy in reduction of gray hair of the 2 groups at each interval (10, 20 and 30 days), experimental (R) hair tonic product was found to be more effective in group 2 (younger age) than group 1 (older age). However, the efficacy in reduction of gray hair was lower than that in reduction of hair loss.<\/p>\n\n\n\n

As for the efficacy of the hair tonic in reducing dandruff, it was found that in group 1, a very high reduction in dandruff was found. The control set (C) and the experimental set (R) were in the range of 40-89% and 55-92% reduction in dandruff efficacy, respectively, with over a period of 30 days with no significance between C and R (Table 5). However, the increasing efficacy in reduction of dandruff was observed from day 10 to 30 in these 2 products indicating that if used continuously, it would reduce dandruff better.<\/p>\n\n\n\n

In group 2, it\nwas found that the reduction of dandruff was much less effective. The use of the\ncommercial hair tonic product (G) and the trial kit (R) led to the efficacy of\nreducing dandruff in the range of 14-28% and 17-23%, respectively, with no\nsignificant difference in results. It was found that the experimental hair\ntonic (R) was more effective in group 1 (older age).<\/p>\n\n\n\n

Previous\nresearch using local herbal extracts as an ingredient in hair tonic products to\nnourish the scalp found that citronella oil at concentrations of 5, 10 and 15% in hair tonic products can reduce dandruff problems. Significantly\nmore effective than the control (no citronella oil) formula at 33, 75, and 51%, respectively (p<\/em> < 0.005) on the 7th<\/sup> day of use. The efficiency increased when\nused until the 14th<\/sup> day, it can reduce dandruff by 52, 81, and 74%, respectively. 17<\/sup> These results of citronella oil in hair tonic are with higher efficacy when compared with our experimental\nR hair tonic product.<\/p>\n\n\n\n

Table 5: Efficacy of hair tonic product against hair loss, gray hair and dandruff<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n
\n

G<\/p>\n<\/td>\n

\n

Hair tonic<\/p>\n<\/td>\n

\n

Hair loss reduction (%)<\/p>\n<\/td>\n

\n

Gray hair reduction (%)<\/p>\n<\/td>\n

\n

Dandruff reduction (%)<\/p>\n<\/td>\n<\/tr>\n

\n

Day 10<\/p>\n<\/td>\n

\n

Day 20<\/p>\n<\/td>\n

\n

Day 30<\/p>\n<\/td>\n

\n

Day 10<\/p>\n<\/td>\n

\n

Day 20<\/p>\n<\/td>\n

\n

Day 30<\/p>\n<\/td>\n

\n

Day 10<\/p>\n<\/td>\n

\n

Day 20<\/p>\n<\/td>\n

\n

Day 30<\/p>\n<\/td>\n<\/tr>\n

\n

1<\/p>\n<\/td>\n

\n

C<\/p>\n<\/td>\n

\n

63.9
\u00b19.5Aa<\/sup><\/p>\n<\/td>\n

\n

70.2
\u00b1 8.8Aa<\/sup><\/p>\n<\/td>\n

\n

77.5
\u00b18.2Aa<\/sup><\/p>\n<\/td>\n

\n

0.5
\u00b10.1Bc<\/sup><\/p>\n<\/td>\n

\n

2.5
\u00b10.2Bb<\/sup><\/p>\n<\/td>\n

\n

14.3
\u00b11.1Aa<\/sup><\/p>\n<\/td>\n

\n

40.0
\u00b116.3Ab<\/sup><\/p>\n<\/td>\n

\n

55.0
\u00b115.7Ab<\/sup><\/p>\n<\/td>\n

\n

88.9
\u00b17.4Aa<\/sup><\/p>\n<\/td>\n<\/tr>\n

\n

R<\/p>\n<\/td>\n

\n

59.4
\u00b19.0Aa<\/sup><\/p>\n<\/td>\n

\n

69.0
\u00b17.2Aa<\/sup><\/p>\n<\/td>\n

\n

70.9
\u00b110.5Aa<\/sup><\/p>\n<\/td>\n

\n

0.5
\u00b10.1Bc<\/sup><\/p>\n<\/td>\n

\n

2.5
\u00b10.2Bb<\/sup><\/p>\n<\/td>\n

\n

20.0
\u00b11.2Aa<\/sup><\/p>\n<\/td>\n

\n

55.0
\u00b115.7Ab<\/sup><\/p>\n<\/td>\n

\n

67.5
\u00b114.9Ab<\/sup><\/p>\n<\/td>\n

\n

91.7
\u00b15.9Aa<\/sup><\/p>\n<\/td>\n<\/tr>\n

\n

2<\/p>\n<\/td>\n

\n

G<\/p>\n<\/td>\n

\n

30.8
\u00b17.7Bb<\/sup><\/p>\n<\/td>\n

\n

40.5
\u00b16.9Bb<\/sup><\/p>\n<\/td>\n

\n

66.5
\u00b16.2Ba<\/sup><\/p>\n<\/td>\n

\n

7.0
\u00b15.2Aa<\/sup><\/p>\n<\/td>\n

\n

17.5
\u00b17.5Aa<\/sup><\/p>\n<\/td>\n

\n

26.0
\u00b110.8Aa<\/sup><\/p>\n<\/td>\n

\n

13.5
\u00b16.6Ba<\/sup><\/p>\n<\/td>\n

\n

31.7
\u00b18.8Ba<\/sup><\/p>\n<\/td>\n

\n

28.3
\u00b110.0Ba<\/sup><\/p>\n<\/td>\n<\/tr>\n

\n

R<\/p>\n<\/td>\n

\n

20.2
\u00b16.1Bb<\/sup><\/p>\n<\/td>\n

\n

46.2
\u00b110.2Ba<\/sup><\/p>\n<\/td>\n

\n

48.0
\u00b110.9Ba<\/sup><\/p>\n<\/td>\n

\n

8.6
\u00b15.3Aa<\/sup><\/p>\n<\/td>\n

\n

24.7
\u00b110.5Aa<\/sup><\/p>\n<\/td>\n

\n

26.9
\u00b110.7Aa<\/sup><\/p>\n<\/td>\n

\n

16.67
\u00b18.6Ba<\/sup><\/p>\n<\/td>\n

\n

23.0
\u00b110.0Ba<\/sup><\/p>\n<\/td>\n

\n

23.0
\u00b110.0Ba<\/sup><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n

Values \u200b\u200bshown are mean \u00b1 SD from experiments on 10 subjects in each group (G1 and G2). Uppercase letters indicate statistically significant differences in columns and lowercase letters in rows (p<\/em> < 0.05) for each efficacy. R = F5 hair tonic, C = Controlled hair tonic and G = Giffarine commercial hair tonic.<\/p>\n\n\n\n

Traditionally, CT, AC and TC herbal extracts are found to be used in medicines. The fruit of CT was incorporated into diets. 21<\/sup> Dried pods of AC are commonly used as herbal medicine such as a laxative. 22<\/sup> TC was used as antidiabetic medicine. 23<\/sup> Upon topical application especially in hair care, the previous work confirmed that the AC plant contains saponins, alkaloids, tannins, anthraquinone glycosides, sugar, and flavonoids. Pod extract cleanses and controls dandruff. The fruits of this plant are used to wash hair, improve hair development, emetic, expectorant, and purgative. 24<\/sup> Saponin is the principal foaming agent. 25<\/sup> It naturally lowers pH and preserves hair oils, keeping them glossy and healthy. It also conditions and strengthens hair. 26<\/sup> In addition, AC and TC plants showed some effects on dermal papilla cells that preventing balding. 27<\/sup> <\/p>\n\n\n\n

Previously, rice bran extract exhibited comparable hair growth-promoting potential to 3% minoxidil in C57BL\/6 mice, indicating that it induced hair follicles to enter the anagen phase. Significant growth was observed in the quantity of hair follicles. 7<\/sup> Rice bran extract, specifically linoleic acid and \u03b3-oryzanol constituent, stimulates hair growth implies that rice by-products may have potential as a treatment for hair loss. 7<\/sup> It was also shown that rice bran ash extract promotes melanin biosynthesis in hair follicle like tissue model and organ culture model. 28<\/sup> Additionally, when applied topically, rice bran enhances the production of growth factors and molecular signals that stimulate cell growth during the anagen phase, such as \u03b2-catenin. It also suppresses enzymes that trigger the transition from anagen to catagen\/telogen phases, like TGF\u03b2 and Type I 5\u03b1-reductase. Rice bran is safe for human usage in cosmetics as it is not genotoxic or cytotoxic. The Amazon.com search produced 119 hair products containing rice, indicating its popularity for over-the-counter purchase. RB promotes hair development by enhancing the expression of growth factors and molecular signals linked to sustaining the anagen phase, reducing inflammation, blocking 5\u03b1-reductase, and stimulating melanogenesis. 29<\/sup><\/p>\n\n\n\n

Our hair tonic was compared to other herbal hair tonics in literature\/the market in terms of formulations and hair care benefits (Table 6). Meduri et al. (2021) formulated a herbal shampoo with fermented rice water for hair growth and hair melanogenesis purposes using different herb extract from our work. 30<\/sup> They highlighted that fermented rice water, which contains inositol, was effective in reducing hair loss. The herbal shampoo was formulated with traditional Indian plants including Hibiscusrosa sinensis<\/em>, Phyllanthus emblica<\/em>, Aloe vera<\/em>, Trigonella foenum graceum<\/em>, making it a suitable, safe, and efficient remedy for hair loss. 30<\/sup> In addition, Hebro herbal hair oil was commercially in the market based on patented DA-5512 formulation comprising 6 herbal extracts including Thea sinensis<\/em> L., Emblica officinalis, Pinus densiflora, Pueraria thunbergiana, Tribulus terrestris, and Zingiber officinale<\/em>. 31<\/sup> Preclinical and clinical research in Korea showed that this DA-5512 formulated extract effectively boosted hair growth and enhanced hair health.<\/p>\n\n\n\n

From another research work in India, Rosmarinus officinalis<\/em> (rosemary 1%) was used in a hair lotion and the hair growth stimulating action was tested on C57BL\/6 mice, with water as the control and 2% minoxidil hair lotion as the standard. It was found that a herbal lotion demonstrated a more pronounced hair growth promotion effect compared to animals treated with the standard medication. 32<\/sup> A few marketed hair tonics in India showed different herbal ingredients with similar hair care benefits. 33<\/sup> Another work using candlenut oil showed that it could promote hair growth in mice. 34<\/sup> It can be seen that herbal hair tonics from different countries (Table 6) tend to use different herbal ingredients for formulations with similar hair care benefits. <\/p>\n\n\n\n

Natural bioactive ingredients\nare more receiving attention as alternatives for hair care application nowadays\nsince oral finasteride, topical minoxidil, and approved medications for the\ntreatment of alopecia may occasionally cause undesirable side effects. The\nresults of our research demonstrated that the bioactivity of Thai herbal plants\nin combination with rice by-products could serve as a functional hair care\ncomponent.<\/p>\n\n\n\n

Table 6: Comparison of herbal hair tonics in literature and in the market<\/strong>.<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
\n

No.<\/strong><\/p>\n<\/td>\n

\n

Hair tonic formulation<\/strong><\/p>\n<\/td>\n

\n

Active constituents<\/strong><\/p>\n<\/td>\n

\n

Hair care benefits<\/strong><\/p>\n<\/td>\n<\/tr>\n

\n

1<\/p>\n<\/td>\n

\n

This work. Thailand.<\/p>\n

1. Catunaregam tomentosa<\/em> (CT)<\/p>\n

2. Acacia concinna<\/em> (AC)<\/p>\n

3. Tinospora crispa<\/em> (TC)<\/p>\n

4. Broken riceberry (BRB)<\/p>\n

5. Crude riceberry oil (CRO)<\/p>\n<\/td>\n

\n

1. saponin 6<\/sup><\/p>\n

2. saponin, including acacinin A, B, C, D and E 35<\/sup><\/p>\n

3. alkaloids, flavonoids, flavone glycosides, triterpenes 11<\/sup><\/p>\n

4-5. \u03b3-oryzanol, tocopherols, \u03b3-linolenic, linoleic, and oleic acids 6,7<\/sup><\/p>\n<\/td>\n

\n

1. hair cleansing [6]\n

2. hair strengthening, hair growth, anti-dandruff 35<\/sup><\/p>\n

3. anti-hair loss 27<\/sup><\/p>\n

4-5. hair growth 7<\/sup>,<\/p>\n

hair melanogenesis 28<\/sup>  <\/p>\n<\/td>\n<\/tr>\n

\n

2<\/p>\n<\/td>\n

\n

Meduri et al. (2021). 30<\/sup> India.<\/p>\n

1. Hibiscusrosa sinensis<\/em><\/p>\n

2. Phyllanthus emblica<\/em><\/p>\n

3. Aloe barbadensis<\/em><\/p>\n

4. Trigonella foenum graceum<\/em><\/p>\n

5. Fermented rice water<\/p>\n<\/td>\n

\n

1. amino acids and vitamin C<\/p>\n

2. tannins, mosaic acid, amino acids, alkaloids, flavonoid glycosides, phenolic glycosides, and terpenoids 36<\/sup><\/p>\n

3. proteolytic enzymes<\/p>\n

4. iron, protein, flavonoids and saponins <\/p>\n

5. inositol<\/p>\n<\/td>\n

\n

1. hair growth<\/p>\n

2. hair pigmentation, hair growth<\/p>\n

3. hair soothing<\/p>\n

4. hair growth<\/p>\n

5. anti-hair loss<\/p>\n

 <\/p>\n<\/td>\n<\/tr>\n

\n

3<\/p>\n<\/td>\n

\n

Yu et al. (2017) 31<\/sup><\/p>\n

Hebro herbal hair oil<\/p>\n

DA-5512 formulation. Korea.<\/p>\n

1.<\/em> Thea sinensis<\/em> L. <\/em><\/p>\n

2.<\/em> Emblica officinalis<\/em><\/p>\n

3.<\/em> Pinus densiflora <\/em><\/p>\n

4.<\/em> Pueraria thunbergiana<\/em><\/p>\n

5.<\/em> Tribulus terrestris<\/em><\/p>\n

6.<\/em> Zingiber officinale<\/em><\/p>\n<\/td>\n

\n

1. epigallocatechin-3-gallate<\/p>\n

2. vitamin C, amino acids, and minerals<\/p>\n

3. vitamins A and C <\/p>\n

4. isoflavones puerarin, daidzein, and genistein 3<\/sup>7<\/sup><\/p>\n

5. saponins, polyphenols, tannins flavonoids, glycosides, and alkaloids 38 <\/sup><\/p>\n

6. alkaloid, phlobotannins, tannins flavanoids, glycosides, and saponins 39 <\/sup><\/p>\n<\/td>\n

\n

1. hair growth<\/p>\n

2. hair growth and pigmentation<\/p>\n

3. anti-hair loss and dandruff <\/p>\n

4. hair pigmentation<\/p>\n

5. hair pigmentation<\/p>\n

6. anti-dandruff<\/p>\n<\/td>\n<\/tr>\n

\n

4<\/p>\n<\/td>\n

\n

Begum et al. <\/em>(2023)<\/em> 32<\/sup> India.<\/em><\/p>\n

1.<\/em> Rosmarinus officinalis<\/em> (rosemary 1%)<\/p>\n<\/td>\n

\n

1. proteins, amino acids, fats, oils, steroids, glycosides, phenolic compounds, flavonoids, volatile oil, and vitamins<\/p>\n<\/td>\n

\n

1. hair growth<\/p>\n<\/td>\n<\/tr>\n

\n

5<\/p>\n<\/td>\n

\n

Goswami & Mukhopadhyay (2022). 33<\/sup> India.<\/p>\n

The EnQ ultimate hair care tonic<\/p>\n

1. Emblica Officinalis<\/em><\/p>\n

2. Eclipta prostrata<\/em><\/p>\n

3. Bacopa monnieri<\/em><\/p>\n<\/td>\n

\n

1. ellagic acid, chebulinic acid, gallic acid, chebulagic acid, leutolin, fatty acids<\/p>\n

2. wedelolactone, wedelic acid, apigenin, luteolin, bamyrin<\/p>\n

3. hersaponin, apigenin, cucurbitacin, D-mannitol<\/p>\n<\/td>\n

\n

1.Anti-dandruff and anti-hair fall<\/p>\n

2. anti-hair fall and anti-gray hair<\/p>\n

3. anti-alllergic<\/p>\n<\/td>\n<\/tr>\n

\n

6<\/p>\n<\/td>\n

\n

Goswami & Mukhopadhyay (2022). 33<\/sup> India.<\/p>\n

Blue nectar hair tonic<\/p>\n

1. Aloe barbadensis<\/em><\/p>\n

2. Rosmarinus officinalis<\/em><\/p>\n

3. Hibiscus rosasinesis<\/em><\/p>\n

4. Eclipta prostrata<\/em><\/p>\n<\/td>\n

\n

1. vitamins, enzymes, minerals, sugars, lignin, saponins, salicylic acids and amino acids<\/p>\n

2. p-<\/em>cymene, linalool, \u03b3-terpinene, thymol, and betapinene<\/p>\n

3. flavonoids, anthocyanins and cyaniding -3, 5- diglucoside<\/p>\n

4. wedelolactone, wedelic acid, apigenin, luteolin, and bamyrin<\/p>\n<\/td>\n

\n

1. hair smoothing<\/p>\n

2. hair growth<\/p>\n

3. hair boom and anti-gray hair<\/p>\n

4. anti-hair fall and anti-gray hair<\/p>\n

 <\/p>\n<\/td>\n<\/tr>\n

\n

7<\/p>\n<\/td>\n

\n

Almas et al. (2022). 34 <\/sup>Indonesia.<\/p>\n

1. Aleurites moluccana<\/em> (Candlenut oil 7%)<\/p>\n<\/td>\n

\n

1. palmitic acid, stearic acid, oleic acid, linoleic acid, and linolenic acid<\/p>\n<\/td>\n

\n

1. hair growth in mice<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n

Satisfaction Survey<\/strong><\/p>\n\n\n\n

From a survey of satisfaction with hair tonic products (Figure 4) in terms of product and price. The results showed that 20 subjects expressed satisfaction with the hair tonic product at a total mean of 6.79 \u00b1 0.32 (out of 9)(Table 7), equivalent to moderate-to-high liking. The highest score was the fragrance of the product (7.20 \u00b1 1.28). The lowest score is the color of the product (6.40 \u00b1 1.67) because buyers do not like the cloudy color of mixing natural extracts with oils from by-products. <\/p>\n\n\n\n\n\n
\"\"<\/td>\n\n

Figure 4: The hair tonic product is in a container with a label.<\/strong><\/p>\n

<\/p>\n

Click here to view Figure<\/a><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n

Table 7: Satisfaction results for the hair tonic product<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\n

Satisfaction attribute<\/strong><\/p>\n<\/td>\n

\n

Mean score<\/strong><\/p>\n<\/td>\n<\/tr>\n

\n

1. Product<\/p>\n<\/td>\n<\/tr>\n

\n

1.1 Appearance<\/p>\n<\/td>\n

\n

6.65 \u00b1 1.14<\/p>\n<\/td>\n<\/tr>\n

\n

1.2 Differences from those in the market<\/p>\n<\/td>\n

\n

6.80 \u00b1 1.20<\/p>\n<\/td>\n<\/tr>\n

\n

1.3 Volume\/content per bottle<\/p>\n<\/td>\n

\n

6.80 \u00b1 1.06<\/p>\n<\/td>\n<\/tr>\n

\n

1.4 Color<\/p>\n<\/td>\n

\n

6.40 \u00b1 1.67<\/p>\n<\/td>\n<\/tr>\n

\n

1.5 Fragrance<\/p>\n<\/td>\n

\n

7.20 \u00b1 1.28<\/p>\n<\/td>\n<\/tr>\n

\n

1.6 Texture<\/p>\n<\/td>\n

\n

6.80 \u00b1 0.89<\/p>\n<\/td>\n<\/tr>\n

\n

1.7 Branding and label<\/p>\n<\/td>\n

\n

6.70 \u00b1 1.53<\/p>\n<\/td>\n<\/tr>\n

\n

1.8 Label complete detail<\/p>\n<\/td>\n

\n

6.85 \u00b1 1.53<\/p>\n<\/td>\n<\/tr>\n

\n

1.9 Reliability<\/p>\n<\/td>\n

\n

6.60 \u00b1 0.75<\/p>\n<\/td>\n<\/tr>\n

\n

1.10 Strength of bottle<\/p>\n<\/td>\n

\n

7.35 \u00b1 1.30<\/p>\n<\/td>\n<\/tr>\n

\n

2. Price<\/p>\n<\/td>\n<\/tr>\n

\n

2.1 Appropriate ($5\/200 mL)<\/p>\n<\/td>\n

\n

6.45 \u00b1 1.70<\/p>\n<\/td>\n<\/tr>\n

\n

2.2 Compared with prices in the market<\/p>\n<\/td>\n

\n

6.85 \u00b1 0.88<\/p>\n<\/td>\n<\/tr>\n

\n

Total<\/p>\n<\/td>\n

\n

6.79 \u00b1 0.32<\/p>\n

Moderate-to-High<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n

Conclusion<\/strong><\/p>\n\n\n\n

BRB, CRO by-products with Thai herbal extracts (CT, AC and TC) can be formulated into hair tonics for hair care with similar reduction efficacy in hair loss, gray hair and dandruff as the commercial hair tonic in Thailand. This novel hair tonic may be a safe way to treat hair loss, gray hair and dandruff. It is paraben-, formaldehyde-, and synthetic dye-free, making it scalp-friendly. Positive response on product satisfaction was received. The trial product had no adverse events to healthy male and female volunteers for 1 month, indicating that it is safe to use topically. This work also presented a new light in adding value to by-products from the agricultural production process and Thai local herbs and also reducing chemical use in hair care products for a sustainable future.<\/p>\n\n\n\n

Acknowledgements<\/strong><\/p>\n\n\n\n

The authors would like to thank\nthe Department of Biotechnology, Faculty of Technology, Mahasarakham\nUniversity, Thailand\nfor laboratory research facilities.<\/p>\n\n\n\n

Conflict\nof Interest
\n<\/strong>All authors disclose that\nthere is no conflict of interest among them.<\/p>\n\n\n\n

Funding\nSource<\/strong><\/p>\n\n\n\n

The authors would like to thank the National Research Council of Thailand through NRCT Grant\nfor New Researcher (Grant no. 555982) for financial support.<\/p>\n\n\n\n

Ethics of Human Experimentation<\/strong><\/p>\n\n\n\n

All\nprocedures performed in studies involving human participants were under the\nethical standards of the institutional and\/or national research committee and\nwith the 1964 Helsinki Declaration and its later amendments or comparable\nethical standards. The study was approved by Human\nEthics Committee at Mahasarakham University, Mahasarakham, Thailand\n(Approval no. 018\/2560).<\/p>\n\n\n\n

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