{"id":42711,"date":"2022-03-31T11:10:35","date_gmt":"2022-03-31T11:10:35","guid":{"rendered":"https:\/\/biomedpharmajournal.org\/?p=42711"},"modified":"2022-05-17T10:41:37","modified_gmt":"2022-05-17T10:41:37","slug":"anti-inflammatory-activity-of-sesamum-indicum-l-seed-extracts-in-experimental-animal-models","status":"publish","type":"post","link":"https:\/\/biomedpharmajournal.org\/staging\/vol15no1\/anti-inflammatory-activity-of-sesamum-indicum-l-seed-extracts-in-experimental-animal-models\/","title":{"rendered":"Anti-Inflammatory Activity of Sesamum Indicum L. Seed Extracts in Experimental Animal Models"},"content":{"rendered":"

Introduction<\/strong><\/p>\n

Inflammation is the defensive response of the body to altered homeostasis due to injury, infection or trauma leading to various systemic and local effects. Although it is a protective\u00a0mechanism, it can result in complications if left untreated.1<\/sup> It may be either acute or chronic, based on the type of the stimulus and the efficacy of the body\u2019s response in terminating it.2<\/sup> Nonsteroidal anti-inflammatory drugs (NSAIDs) and Glucocorticoids are commonly\u00a0employed for treating inflammatory conditions. They have several drawbacks such as short duration of action and adverse effects. NSAIDs are associated with adverse effects such as gastric mucosal damage and ulcer whereas glucocorticoids can cause osteoporosis, muscle\u00a0weakness, cataract etc on long term use.3,4<\/sup> Hence the search for novel anti-inflammatory agents with better safety profile is on-going.<\/p>\n

Medicinal plants are an abundant source of new drugs. Sesamum indicum<\/em>\u00a0L. (Sesame) belongs to the order tubiflorae and family Pedaliaceae. It is known by various names such as gingelly, til, benne seed etc. It is highly resistant to oxidation and rancidity.5,6<\/sup> It has health promoting property. Its seeds are rich source of nutrients such as copper, calcium, iron, magnesium, phosphorous, manganese, vitamin B1, zinc etc.7<\/sup> The seeds contains phytochemical compounds like lignans, the important ones are sesamin, sesamol, sesaminol\u00a0and sesamolin. The biological activities of sesame are attributed to these lignans.8<\/sup> The cholesterol lowering property of sesame has been widely reported.9-12<\/sup> Previous studies have documented the antioxidant, antiaging, antihypertensive and anticancer activities of sesame.13-18<\/sup><\/p>\n

The current study was conducted to investigate the anti-inflammatory activity of the two extracts of Sesamum indicum<\/em>\u00a0L. seeds (ethanolic and hexane) by two models of inflammation \u2013 carrageenan induced and formalin induced paw edema \u2013 in Wistar rats.<\/p>\n

Materials and methods<\/strong><\/p>\n

Preparation of plant extracts<\/strong><\/p>\n

The Sesamum indicum<\/em>\u00a0L. seeds were sun dried for 5 days. They were powdered in a mortar. The dry powder was taken in soxhlet apparatus and extraction was done using different\u00a0solvents like ethanol and n-hexane. Rotary evaporator was used for concentrating the filtrate. After extraction excess solvent was distilled at 80\u00b0C. The extract so obtained was stored in sterile bottles.<\/p>\n

Chemicals<\/strong><\/p>\n

The standard drug – indomethacin was purchased from Dabur pharma Ltd, Tarapur, Thane. Ethanol and other chemicals were purchased from Sigma Aldrich Pvt Ltd, Bengaluru, India. All chemicals were of analytical grade.<\/p>\n

Animals<\/strong><\/p>\n

Albino Wistar rats of either sex weighing 130-150 g were procured from central animal house, Sri Kaliswari college, Sivakasi, India. They were housed in polypropylene cages and\u00a0maintained at 12hr :12hr light dark cycle. Standard pellets and water were provided ad libitum. The care and maintenance of experimental animals complied with the The Indian\u00a0National Science Academy (INSA) guidelines. Institutional Animal Ethical Committee approved the study protocol.<\/p>\n

Experiment\u00a0<\/strong><\/p>\n

The animals were divided into 5 groups. Group 1 was given normal saline (0.1 ml\/kg per oral) and served as control. Group 2 was administered the standard drug – Indomethacin (20 mg\/kg intraperitoneally). For Carrageenan induced paw edema model, the test groups\u00a0(Groups 3, 4 and 5) were administered ethanolic extract of Sesamum indicum<\/em>\u00a0L. at three doses – 150, 200 and 250 mg\/kg respectively orally, whereas for Formalin induced paw\u00a0edema model, the test groups were given hexane extract of Sesamum indicum<\/em>\u00a0L. orally at the same doses (150, 200 and 250 mg\/kg respectively). Paw volume of all animals was measured before inducing edema.<\/p>\n

Carrageenan induced paw edema<\/strong><\/p>\n

This test was done following the method of Winter et al.19<\/sup> Paw edema was induced by injecting 0.1 ml of freshly prepared 1% w\/v suspension of carrageenan in normal saline into\u00a0the subplantar region of left hind paw. Carrageenan was injected 1 hour after the drug and extract administration. Paw volume was measured at various time intervals (1,2,3,4 hrs) after\u00a0carrageenan injection using a Plethysmometer. Mean percentage change in paw volume was calculated in all groups and percentage of inhibition of edema was compared with standard.<\/p>\n

Formalin induced paw edema<\/strong><\/p>\n

A sub-plantar injection of 0.1 ml of 2% formalin was given to the left hind paw of each rat after one hour of the drug and extract administration. Paw volume was measured at hourly\u00a0intervals from 1st<\/sup> to 4th<\/sup> hour using a Plethysmometer. Mean percentage change in paw volume of all experimental animals was calculated and comparison of percentage of\u00a0inhibition of edema with standard was done.20<\/sup><\/p>\n

The percentage inhibition of paw edema for both the methods was calculated by the formula: 100 x (1- Vt\/Vc)<\/p>\n

Statistical analysis<\/strong><\/p>\n

The data was expressed as mean \u00b1 standard error of mean (SEM). The data was analysed by one way ANOVA and Dunnets test as post hoc. P value < 0.05 was considered significant.<\/p>\n

Results<\/strong><\/p>\n

Effect on Carrageenan induced paw edema in rats<\/strong><\/p>\n

Carrageenan injection into the hind paw of rats resulted in a progressive increase in paw volume. In group 2 rats which were administered Indomethacin (20 mg\/kg), there was a\u00a0significant inhibition of edema when compared to control group (p < 0.05). The percentage inhibition was maximum at 4th<\/sup> hour and there was 80% inhibition. Among the test group rats\u00a0(Groups 3, 4, 5), significant inhibition of edema was observed only in Group 5 rats (Sesamum indicum<\/em>\u00a0L. ethanolic extract 250 mg\/kg) at 4th<\/sup> hour (p < 0.05). The percentage inhibition of edema at 4th<\/sup> hour was 77%. (Table 1).<\/p>\n

Table 1: Anti-inflammatory activity of Sesamum indicum<\/em>\u00a0L. ethanolic extract in Carrageenan induced paw edema<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n
Treatment groups<\/strong><\/p>\n

\u00a0<\/strong><\/td>\n

Treatment given<\/strong><\/td>\nPaw volume in ml (% inhibition of edema)<\/strong><\/td>\n<\/tr>\n
0 hr<\/strong><\/td>\n1 hr<\/strong><\/td>\n2 hr<\/strong><\/td>\n3 hr<\/strong><\/td>\n4 hr<\/strong><\/td>\n<\/tr>\n
Group 1 (Control)<\/td>\nNormal saline (0.1 ml\/kg)<\/td>\n1.6\u00b1 0.01<\/td>\n2.8 \u00b1 0.05<\/td>\n3.1\u00b10.09<\/td>\n7.1 \u00b1 0.1<\/td>\n8.1 \u00b1 0.07<\/td>\n<\/tr>\n
Group 2<\/p>\n

(Standard)<\/td>\n

Indomethacin (20 mg\/kg)<\/td>\n1.6\u00b1 0.05<\/td>\n1.9 \u00b1 0.12 (32)<\/td>\n2.0\u00b1 0.07 (35)<\/td>\n5.9 \u00b1 0.03 (17)<\/td>\n1.6 \u00b1 0.01* (80)<\/td>\n<\/tr>\n
Group 3<\/td>\nSesamum indicum<\/em>\u00a0L. extract (150 mg\/kg)<\/td>\n1.6\u00b1 0.03<\/td>\n1.8 \u00b1 0.012 (36)<\/td>\n5.1 \u00b1 0.06<\/td>\n8.1\u00b1 0.04<\/td>\n4.1 \u00b1 0.03 (49)<\/td>\n<\/tr>\n
Group 4<\/td>\nSesamum indicum<\/em>\u00a0L. extract (200 mg\/kg)<\/td>\n1.6\u00b1 0.01<\/td>\n1.7\u00b1 0.04 (39)<\/td>\n4.6\u00b1 0.07<\/td>\n7.7\u00b1 0.03<\/td>\n3.2\u00b1 0.1 (60.5)<\/td>\n<\/tr>\n
Group 5<\/td>\nSesamum indicum<\/em>\u00a0L. extract (250 mg\/kg)<\/td>\n1.6\u00b1 0.04<\/td>\n1.1 \u00b1 0.12 (61)<\/td>\n3.4\u00b1 0.03<\/td>\n4.4\u00b1 0.07 (38)<\/td>\n1.9\u00b1 0.02* (77)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Data is presented as mean \u00b1 SEM. *=P < 0.05, **=P < 0.01 compared with control.<\/p>\n

Effect on Formalin induced paw edema in rats<\/strong><\/p>\n

The subplantar injection of Formalin into hind paw of the animals caused an increase in paw volume which progressed from 1st<\/sup> hour to 4th<\/sup> hour. There was a significant inhibition of\u00a0edema in Group 2 rats which were administered Indomethacin (20 mg\/kg) (p < 0.05). This effect was maximum at 4th<\/sup> hour and percentage of inhibition of edema was 89%. The\u00a0Sesamum indicum<\/em>\u00a0L. hexane extract at all three doses (150 mg\/kg, 200 mg\/kg and 250 mg\/kg) has caused significant inhibition of edema at 4th<\/sup> hour (p < 0.05). The percentage of\u00a0edema inhibition in Group 2, 3 and 4 animals was 82%, 88% and 89% respectively. The percentage inhibition of the extract at 250 mg\/kg (Group 5) is comparable to Indomethacin. (Table 2).<\/p>\n

Table 2: Anti-inflammatory activity of Sesamum indicum<\/em>\u00a0L. hexane extract in Formalin induced paw edema<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n
Treatment groups<\/strong><\/td>\nTreatment given<\/strong><\/td>\nPaw volume in ml (% of inhibition of edema)<\/strong><\/td>\n<\/tr>\n
0 hr<\/strong><\/td>\n1 hr<\/strong><\/td>\n2 hr<\/strong><\/td>\n3 hr<\/strong><\/td>\n4 hr<\/strong><\/td>\n<\/tr>\n
Group 1 (Control)<\/td>\nNormal saline (0.1 ml\/kg)<\/td>\n1.5 \u00b1 0.51<\/td>\n3.2 \u00b1 0.89<\/td>\n3.9\u00b1 0.01<\/td>\n7.8 \u00b1 0.03<\/td>\n8.9 \u00b1 0.12<\/td>\n<\/tr>\n
Group 2 (Standard)<\/td>\nIndomethacin (20 mg\/kg)<\/td>\n1.5 \u00b1 0.80<\/td>\n1.6 \u00b10.02 (50)<\/td>\n1.7\u00b11.10 (56)<\/td>\n3.3 \u00b1 1.9 (58)<\/td>\n1.0 \u00b1 0.08* (89)<\/td>\n<\/tr>\n
Group 3<\/td>\nSesamum indicum<\/em>\u00a0L. extract (150 mg\/kg)<\/td>\n1.5 \u00b1 0.01<\/td>\n1.6 \u00b1 0.81 (50)<\/td>\n4.4\u00b1 0.73<\/td>\n6.4\u00b1 0.12 (18)<\/td>\n1.6\u00b1 0.11* (82)<\/td>\n<\/tr>\n
Group 4<\/td>\nSesamum indicum<\/em>\u00a0L. extract (200 mg\/kg)<\/td>\n1.5 \u00b1 0.62<\/td>\n1.4\u00b1 0.43 (56)<\/td>\n3.8\u00b1 0.02 (3)<\/td>\n5.1\u00b1 0.03 (35)<\/td>\n1.1\u00b1 0.01* (88)<\/td>\n<\/tr>\n
Group 5<\/td>\nSesamum indicum<\/em>\u00a0L. extract (250 mg\/kg)<\/td>\n1.5 \u00b1 0.53<\/td>\n1.3 \u00b1 0.13 (59)<\/td>\n3.5 \u00b1 0.30 (10)<\/td>\n4.2\u00b1 0.18 (46)<\/td>\n1.0 \u00b1 0.02* (89)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Data is presented as mean \u00b1 SEM. *=P < 0.05, **=P < 0.01 compared with control<\/p>\n

Discussion<\/strong><\/p>\n

The current study evaluated the anti-inflammatory activity of ethanolic extract and hexane extract of Sesamum indicum<\/em>\u00a0L. seed in Carrageenan induced paw edema and\u00a0Formalin induced paw edema respectively in Albino Wistar rats. Carrageenan induced paw edema is used to study acute and subacute phases of inflammation while formalin induced\u00a0paw edema is employed to evaluate chronic inflammation in rodents.21,22<\/sup><\/p>\n

Carrageenan is a widely used inflammogen because of its greater reproducibility. It is a non-antigenic phlogisitc agent without any systemic effects.23<\/sup> This model is used as a\u00a0preliminary screening test for anti-inflammatory compounds as it involves multiple mechanisms.24<\/sup> Stimulation of phospholipase A2 by carrageenan initiates the early phase of\u00a0inflammation while its cytotoxic effects lead to progression of the inflammation.25<\/sup> Inflammatory response induced is biphasic. The initial phase (0-1 hr) involves release of\u00a0mediators like histamine, serotonin and kinins, while the latter phase (1-5 hr) involves prostaglandin release.26,27<\/sup> These cascade of events progress to exudate formation. The\u00a0biphasic response of this model permits the prediction of the probable biological targets of test compound.28<\/sup> This model is frequently utilised for testing both steroidal and non-steroidal\u00a0anti-inflammatory drugs.29<\/sup> The clinically effective anti-inflammatory drugs alter the latter phase. This method has a predictive value for screening compounds which cause inhibition of\u00a0mediators of acute inflammation.30<\/sup> In this study the Sesamum indicum<\/em>\u00a0L. ethanolic extract at a dose of 250 mg\/kg significantly inhibited the paw edema volume in the latter phase which\u00a0is mediated by prostaglandins. This suggests that Sesamum indicum<\/em>\u00a0L. ethanolic extract may have a protective role against inflammation induced by Carrageenan.<\/p>\n

Formalin-induced paw edema is an appropriate model to investigate chronic anti-inflammation because of its close resemblance to human arthritis.31 <\/sup>Formalin also induces\u00a0biphasic inflammation. The early neurogenic phase involves bradykinin and substance-P while the latter inflammatory phase involves histamine, 5-HT, prostaglandins, bradykinin and\u00a0cytokines like IL-1\u03b1, IL-6, TNF-\u03b1, eicosanoids, and Nitric Oxide.32,33<\/sup> Based on the ability of the test compound to inhibit the early phase, the latter phase or on both the phases, it can be\u00a0predicted whether the anti-inflammatory effect involves central or peripheral components. The centrally acting drugs such as opioids cause equal suppression of both the phases,\u00a0whereas the drugs acting peripherally like NSAIDs and corticosteroids cause exclusive inhibition of latter phase.34<\/sup> In the current study, Sesamum indicum<\/em>\u00a0L. hexane extract has\u00a0caused significant inhibition of paw edema in a dose dependent manner. The percentage inhibition of the extract at 250 mg\/kg was comparable to Indomethacin. These results suggest\u00a0a possible protective effect of hexane extract of Sesamum indicum<\/em>\u00a0L. against formalin induced inflammation.<\/p>\n

The results of our study are consistent with other studies confirming the anti-inflammatory activity of Sesamum indicum<\/em>\u00a0L. (sesame).35-37 <\/sup>Previous studies have reported\u00a0that Sesamin, which is a major lignan present in Sesamum indicum<\/em>\u00a0L. seeds is a\u00a0potent and specific inhibitor of delta 5 desaturase enzyme involved in biosynthesis of polyunsaturated\u00a0fatty acids. This leads to dihomo-linolenic acid accumulation and displacement of arachidonic acid, thereby reducing the synthesis of pro-inflammatory prostaglandins.38,39<\/sup>\u00a0Hence the anti-inflammatory activity exhibited by Sesamum indicum<\/em>\u00a0L. seed extracts in the present study may be due to its lignan (Sesamin) content.<\/p>\n

Conclusion<\/strong><\/p>\n

The findings of our study confirm the anti-inflammatory activity of ethanolic and hexane extracts of Sesamum indicum<\/em>\u00a0L. seeds in Carrageenan induced inflammation and\u00a0Formalin induced inflammation models respectively in Wistar rats. Sesamin, the major lignan in Sesamum indicum<\/em>\u00a0L. seeds can be a potential target for development of novel anti-inflammatory drug.<\/p>\n

Acknowledgement<\/strong><\/p>\n

We thank Anjukam, Senthamari Selvi and Geetha G. for their help and support.<\/p>\n

Conflict of Interest<\/strong><\/p>\n

There are no conflict of Interest.<\/p>\n

Funding Sources<\/strong><\/p>\n

There is no funding source.<\/p>\n

References<\/strong><\/p>\n

    \n
  1. Goyal S, Gupta MC and Verma S. Anti-inflammatory effects of Morphine and Gabapentin, alone and in combination, in rats. Pharmacologia 2015; 6(3): 106-9.
    \nCrossRef<\/a><\/li>\n
  2. Arzi A, Olapour S, Yaghooti H, Karampour NS. Effect of Royal Jelly on Formalin Induced-Inflammation in Rat Hind Paw. Jundishapur J Nat Pharm Prod. 2015; 10(1): e22466.
    \n    CrossRef<\/a><\/li>\n
  3. Brenner GM, Stevens CW. Drugs for pain, inflammation and arthritic disorders. In: Pharmacology. 4th edition, Philadelphia, Saunders, 2014: 314-329.
    \n    CrossRef<\/a><\/li>\n
  4. Satyanarayanasetty D,\u00a0Pawar K,\u00a0Nadig Pand\u00a0Haran Multiple Adverse Effects of Systemic Corticosteroids: A Case Report. J Clin Diagn Res. 2015; 9(5): FD01\u2013FD02.<\/li>\n
  5. Bedigian D, Harlan JR. Evidence for cultivation of sesame in the ancient world.\u00a0Econ Bot.\u00a01986; 40 :137\u201354.
    \n    CrossRef<\/a><\/li>\n
  6. Pathak N,\u00a0Rai AK,\u00a0Kumari R,\u00a0and\u00a0Bhat Value addition in sesame: A perspective on bioactive components for enhancing utility and profitability. Pharmacogn Rev. 2014 ; 8(16): 147\u201355.
    \n    CrossRef<\/a><\/li>\n
  7. Anilakumar KR, Pal A, Khanum F, Bawa AS. Nutritional, Medicinal and Industrial Uses of Sesame (Sesamum indicum L.) Seeds – An Overview. Agric. conspec. sci. 2010; 75 (4): 159-68.<\/li>\n
  8. Wu MS, Aquino LBB, Barbaza MYU, Hsieh CL, De Castro-Cruz KA, Yang LL and Tsai PW. Anti-Inflammatory and Anticancer Properties of Bioactive Compounds from Sesamum indicum L.\u2014A Review. Molecules 2019; 24, 4426: 1-28.
    \n    CrossRef<\/a><\/li>\n
  9. Hirata F., Fujita K., Ishikura Y. (1996). Hypocholesterolemic eff ect of sesame lignan in humans. Atheroscler 122(1): 135-36.
    \n    CrossRef<\/a><\/li>\n
  10. Ogawa H., Sasagawa S., Murakami T., Yoshizumi H. (1995). Sesame lignans modulate cholesterol metabolism in the stroke-prone spontaneously hypertensive rat. Clin Exp Pharmacol Physiol Suppl 1: 10-12
    \n    CrossRef<\/a><\/li>\n
  11. Visavadiya NP, Narasimhacharya AV. Sesame as a hypocholesteraemic and antioxidant dietary component.\u00a0Food Chem Toxicol.\u00a02008;46:1889\u201395.
    \n    CrossRef<\/a><\/li>\n
  12. Isha, D.; Milind, P. Eat til and protect dil. Int. Res. J. Pharm. 2012, 3, 54\u201357.<\/li>\n
  13. Sirato-Yasumoto S. M. J., Katsuta Y., Okuyama Y., Takahashi Ide T. (2001). Eff ect of sesame seeds rich in sesamin and sesamolin on fatty acid oxidation in rat liver. J Agri Food Chem 49: 2647-51.
    \n    CrossRef<\/a><\/li>\n
  14. Suja KP, Jayalekshmy A, Arumughan C. Free radical scavenging behavior of antioxidant compounds of sesame (Sesamum indicum<\/em>) in DPPH(*) system.\u00a0J Agric Food Chem.\u00a02004;52:912\u20135.
    \n    CrossRef<\/a><\/li>\n
  15. Shyu Y. S. S., Hwang L. S. (2002). Antioxidant activity of white and black sesame seeds and their hull fractions. Food Res Inter 35(4): 357-65.
    \n    CrossRef<\/a><\/li>\n
  16. Furumoto and K. Nishimoto, \u201cIdentification of a characteristic antioxidant, anthrasesamone F, in black sesame seeds and its accumulation at different seed developmental stages,\u201d Bioscience, Biotechnology and Biochemistry, 2016; 80(2): 350\u20135.
    \n    CrossRef<\/a><\/li>\n
  17. Nakano D, Kurumazuka D, Nagai Y, Nishiyama A, Kiso Y, Matsumura Y. Dietary sesamin suppresses aortic NADPH oxidase in DOCA salt hypertensive rats.\u00a0Clin Exp Pharmacol Physiol.\u00a02008;35:324\u20136.
    \n    CrossRef<\/a><\/li>\n
  18. Yokota T, Matsuzaki Y, Koyama M, Hitomi T, Kawanaka M, Enoki-Konishi M, et al. Sesamin, a lignan of sesame, down-regulates cyclin D1 protein expression in human tumor cells.\u00a0Cancer Sci.\u00a02007;98:1447\u201353.
    \n    CrossRef<\/a><\/li>\n
  19. Winter CA, Risley EA, Nuss GW. Carrageenan-induced edema in hind paw of the rat as an assay for anti-inflammatory drugs. Proc Soc Exp Biol Med 1962; 111: 544-7.
    \n    CrossRef<\/a><\/li>\n
  20. Chau TT. Analgesic Testing In Animal Models. In: Pharmacological Methods in the Control of Inflammation, Chang JY and AJ Lewis (ed.). Alan R. Liss, Inc., New York (1989) 195-212.<\/li>\n
  21. Mahajan, A.; Hardyal, S.; Tung, B. Effect of concurrent use of cimetidine and anti-inflammatory agents in experimental models of peptic ulcer and inflammation. Indian J. Pharmacol. 1984, 16, 132. 109.<\/li>\n
  22. Juma,K.M.;Ahmed,Z.A.;Numan,I.T.;Hussain, S.A.R. Dose-dependent anti-inflammatory effect of silymarin in experimental animal model of chronic inflammation. Afr. J. Pharm. Pharmacol. 2009, 3, 242\u2013247.<\/li>\n
  23. Sarkhel, S. Evaluation of the anti-inflammatory activities of Quillajasaponaria Mol. saponin extract in mice. Toxicol. Rep. 2016, 3, 1\u20133.Larsen GL, Henson PM. Mediators of inflammation. Annu Rev Immunol. 1983; 1: 335-59.
    \n    CrossRef<\/a><\/li>\n
  24. Winyard, P.G.; Willoughby, D.A.; Morris, C.J. Carrageenan-Induced Paw Edema in the Rat and Mouse. In Inflammation Protocols; Springer Science and Business Media LLC: Berlin\/Heidelberg, Germany, 2003; Volume 225, pp. 115\u2013122. 161.<\/li>\n
  25. Fernandez, A.; Alvarez, A.; Garc\u00eda, M.; S\u00e1enz, M. Anti-inflammatory effect of Pimenta racemosa var. ozua and isolation of the triterpene lupeol. Il Farm. 2001, 56, 335\u2013338.
    \n    CrossRef<\/a><\/li>\n
  26. Brooks PM, Day RO. Nonsteroidal anti-inflammatory drugs: differences and similarities. N Engl J Med. 1991; 324: 1716-25.
    \n    CrossRef<\/a><\/li>\n
  27. Vane J, Botting R. Inflammation and the mechanism of action of anti-inflammatory drugs. FASEBJ. 1987;1: 89-96.
    \n    CrossRef<\/a><\/li>\n
  28. Seibert,K.;Masferrer,J.L.Roleofinduciblecyclooxygenase(COX-2)in inflammation. Receptor1994,4,17\u201323.<\/li>\n
  29. Wu Y, Zhou X, Li X, Song L, Wu X, Lin W. Evaluation of Anti-inflammatory activity of the total flavanoids of Lagerra ptoerodonta on acute and chronic inflammation models. Phytother Res 2006; 20: 585-590.
    \n    CrossRef<\/a><\/li>\n
  30. Badole S, Zanwar A, Ghule A, Ghosh P, Bodhankar S. Analgesic and anti-inflammatory activity of alcoholic extract of stem bark of Pongamia pinnata (L.) Pierre. Biomedicine Aging Pathol 2011; 2 (1): 9-23.
    \n    CrossRef<\/a><\/li>\n
  31. Greenwald RA. Animal model for the evaluation of arthritic drugs. Methods Find Exp Clin Pharmacol. 1991;13: 75\u201383.<\/li>\n
  32. Segawa, S.; Takata, Y.; Kaneda, H.; Watari, J. Effects of a Hop Water Extract on the Compound 48\/80-Stimulated Vascular Permeability in ICR Mice and Histamine Release from OVA-Sensitized BALB\/c Mice. Biosci. Biotechnol. Biochem. 2007, 71, 1577\u20131581.
    \n    CrossRef<\/a><\/li>\n
  33. Fu KY, Light AR, Maixner W. Long-lasting inflammation and longterm hyperalgesia after subcutaneous formalin injection into the rat hindpaw. J Pain. 2001;2(1):2\u201311.16.
    \n    CrossRef<\/a><\/li>\n
  34. Lalrinzuali, K.; Vabeiryureilai, M.; Jagetia, G.C. Investigation of the Anti-Inflammatory and Analgesic Activities of Ethanol Extract of Stem Bark of Sonapatha Oroxylumindicum In Vivo. Int. J.Inflamm 2016. 1\u20138.
    \n    CrossRef<\/a><\/li>\n
  35. Saleem TSM et al. Analgesic, anti-pyretic and anti-inflammatory activity of dietary sesame oil in experimental animal models. Pharmacologia 2011. 2(6): 172-77.
    \n    CrossRef<\/a><\/li>\n
  36. Monteiro EMH et al. Antinociceptive and Anti-Inflammatory Activities of the Sesame Oil and Sesamin. Nutrients 2014. 6(5): 1931-44.
    \n    CrossRef<\/a><\/li>\n
  37. Ismail M, Hasan H , El-Orfali Y, Ismail H and Khawaja G. Anti-Inflammatory, Antioxidative, and Hepatoprotective Effects of Trans \u03949-Tetrahydrocannabinol\/Sesame Oil on Adjuvant-Induced Arthritis in Rats. Evidence-Based Complementary and Alternative Medicine 2018. 1-13.
    \n    CrossRef<\/a><\/li>\n
  38. Shimizu S, Akimoto K, Shinmen Y, Kawashima H, Sugano M, Yamada H. Sesamin is a potent and specific inhibitor of delta 5 desaturase in polyunsaturated fatty acid biosynthesis.\u00a0Lipids.\u00a01991;26:512\u20136.
    \n    CrossRef<\/a><\/li>\n
  39. Jeng KCG and Hou RCW. Sesamin and sesamolin: Nature\u2019s therapeutic lignans. Curr. Enz. Inhibit, 2005; 1:11-20.
    \n    CrossRef<\/a><\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

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