{"id":40627,"date":"2021-09-30T10:10:15","date_gmt":"2021-09-30T10:10:15","guid":{"rendered":"https:\/\/biomedpharmajournal.org\/?p=40627"},"modified":"2021-10-12T04:36:36","modified_gmt":"2021-10-12T04:36:36","slug":"isolation-and-partial-purification-of-bioactive-compounds-from-sponge-sigmadocia-fibulata-schmidt-collected-from-west-coast-of-mumbai-india","status":"publish","type":"post","link":"https:\/\/biomedpharmajournal.org\/staging\/vol14no3\/isolation-and-partial-purification-of-bioactive-compounds-from-sponge-sigmadocia-fibulata-schmidt-collected-from-west-coast-of-mumbai-india\/","title":{"rendered":"Isolation and Partial Purification of Bioactive Compounds from Sponge Sigmadocia Fibulata (Schmidt) Collected from West Coast of Mumbai, India"},"content":{"rendered":"

Introduction<\/strong><\/p>\n

Sponge contains a large number of unique and diverse natural products. For medicinal use many sponges have contributed the unique bioactive compounds.\u00a0 The arrays of secondary metabolites of sponges are ranging from amino acids and nucleotides to peroxides and sterols 1<\/sup>.\u00a0Many bioactive compounds have immense pharmacological properties, which includes anti tumor, viral, fungal, anti-bacterial and many others. Some of these properties are now in clinical and preclinical trials2<\/sup>.<\/sup><\/strong> Many natural products were also played important role in biological\u00a0systems such as reproduction and play a crucial role as defense against predators, competitions for space, prevention of fouling and useful for the preparation of many antibiotics such as plakortin3<\/sup>\u00a0<\/sup><\/strong>and manoalide from marine Sponges4<\/sup>. <\/sup><\/strong>From living marine fauna, more than 60% of\u00a0potentially bioactive compounds have been obtained till 1999 of which 70% of the bioactive compounds were isolated from sponges5<\/sup>. <\/sup><\/strong>Throughout the globe, around 15000 different species of sponges were identified of which around 150 species were reported as freshwater sponges.\u00a0Nearly 5000 different bioactive compounds were extracted from 500 different species of sponges of which 17% of sponges have commercial and traditional use including cosmetics6<\/sup>. Amongst the marine invertebrates, around 486 different species of sponges were reported in Indian\u00a0waters7<\/sup>. In sponges, the class Demospongiae is well known for the production of secondary metabolites which have diverse function8<\/sup>. The interest of Marine natural products as one of the few de novo sources of drug discovery worldwide 9<\/sup>. However, in India, the bioactive potential\u00a0from marine animals has been studied very scanty. In India the work carried on bioactive compounds by NIO Goa and others were from the coast of south and south- east India 10<\/sup>. \u00a0The work on bioactive compounds from west coast of India has been carried out by 11<\/sup> CIFE,\u00a0Mumbai, and no efforts have gone into unraveling the details on bioactive compounds.12<\/sup> studied the bioactive compounds from intertidal crab Leptodius exratus<\/em> from Nariman point, west coast of Mumbai. Therefore an initial effort has been initiated for the isolation and partial purification\u00a0of secondary metabolites from marine sponge S.\u00a0 fibulata<\/em> (schmidt) from west coast of Mumbai.<\/p>\n

Materials and Methods<\/strong><\/p>\n

Samples collection<\/strong><\/p>\n

The sponges S. fibulata<\/em> was collected from Nariman Point, west coast of Mumbai during low tides.\u00a0 The sponges were brought to the laboratory in seawater and then washed with distilled water and sun dried.<\/p>\n

Identification of sponges<\/strong><\/p>\n

Initial Preliminary identification was done by referring the literature. The shape and size of spicules were studied to confirm the specimen. The confirmation of identification was done by Dr. P.A. Thomas, Central Marine Fisheries Research Institute (CMFRI), Thiruvananthapuram, Kerala.<\/p>\n\n\n\n
\"Vol14No3_Iso_Bha_fig1\"<\/a><\/td>\nfigure\u00a01: Specimen of marine sponge S. fibulata\u00a0<\/em>(Schmidt, 1862) collected from Mumbai coast<\/strong>.<\/p>\n

Click here to view figure\u00a0<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Preparation of Sponge Extracts<\/strong><\/p>\n

The sponge extract was obtained by using the method as proposed by Braekman et al., 1992 with some modifications.<\/p>\n

The extraction of sponge sample was done by following the method of13<\/sup> with some modifications. 10 grams of sponge sample was added in 10 mL of methanol and kept standing for 24 hrs in conical flask by covering the mouth of the conical flask with stopper. After 24 hrs\u00a0the sponges squeezes and made the suspension in methanol. The sample was then filtered through Whatman filter paper No.1. The sample so obtained was evaporated at low pressure by using Rotary Vacuum Evaporator at 45\u2070C. The resultant compound so obtained was subjected\u00a0to Millipore filter and finally dried in a vacuum desiccator and stored at 4\u2070C in a refrigerator till further use.<\/p>\n\n\n\n
\"Vol14No3_Iso_Bha_fig2\"<\/a><\/td>\nFigure 2: Crude extract of marine sponge S. fibulata\u00a0<\/em>(Schmidt, 1862) collected from Mumbai coast.<\/strong><\/p>\n

Click here to view figure\u00a0<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Ethical approval<\/strong><\/p>\n

Ethical approval for the collection of S.<\/em> fibulata<\/em> was sought from the Maharashtra State Biodiversity Board, Nagpur and the voucher specimen of S.<\/em> fibulata<\/em> was sent to the repository center at NIO Goa, India, to deposit the sample. The Voucher numbers of the said specimen is 1-NIO1006\/18 (S. fibulata).<\/em><\/p>\n

TLC Analysis<\/strong><\/p>\n

Model Chem. Tech, HPTLC (High Performance Thin Layer Chromatography) available at Ancrom test lab. Mulund, Mumbai was used for the analysis of samples.<\/p>\n

The aluminum TLC plates pre- coated with silica gel (60 F254) with 0.5 mm thickness were used for the experiment. The dried plates were charged and 10 mL of sponge crude extract was loaded on the plate and allowed the sample to dry at 100oC. The loaded plate then emerged in the\u00a0saturated twin trough chamber containing Toluene: Ethyl acetate: Diethylamine (7:2:1) (v\/v) as a mobile phase. The sample spots were run up to 9cm length in the mobile phase. The plate was removed from twin trough chamber and dried at110oC and dragendroff reagent was sprayed to\u00a0develop the color spots. The spots were so obtained were observed under Deutorium lamp at 254 nm of the densitometric scanner. The spots scrapped off and dissolved in methanol. The pure samples were so obtained were filtered through Millipore filter and evaporated to dryness to get\u00a0the pure compounds. The pure samples further processes for analytical studies.<\/p>\n

GC-MS (Gas chromatography \u2013 Mass Spectrometry) Analysis<\/strong><\/p>\n

The dried powdered form samples were analyzed at IIT (SAIF) Powai, Mumbai. Analysis was performed with Jeol model Accu TOF GCV, GC MS (EI +ve or \u2013ve) was used. The mass range of the spectrometer was ranges from 10 – 2000 amu at a mass resolution \u2013of 6000.<\/p>\n

FTIR (Fourier Transform Infrared Spectroscopy) Analysis<\/strong><\/p>\n

The dried isolated powdered sponge samples analyzed at IIT (SAIF) Powai, Mumbai. Analysis was performed with Bruker instrument corporation Germany make, 3000 Hyperion Microscope with Vertex 80 FTIR model was used. The scanning was done in the range of 4000-900cm-1.\u00a0Analytical grade solvents and chemicals were used from M\/S. S.D. fine chemicals, Thane, India.<\/p>\n

Results and Discussion<\/strong><\/p>\n

Many marine sponges harbor a large number of secondary metabolites possessing a broad-spectrum of pharmacological applications have not yet been discovered. There are few sponges such as Hyrtios <\/em>species, H.erectus, H.reticulatus, H gumminae ,<\/em> and H.tubulatus<\/em> discovered\u00a0bioactive compounds which have promising antifungal, antibacterial and antitumor\u00a0 properties14<\/sup>.\u00a0\u00a0\u00a0 \u00a0The study carried out by 15<\/sup> have obtained\u00a0 many compounds as Sterols (22ee)-24a-methyl-cholest-48(9), 22(23)-triene-3a,713-diol(1) and (22e)-24 amethylergost-6, 22(23)-diens-5a, 8a-epidioxy-3\/3-o1(2) a fatty-acid nonadecanoic acid and aoily ester methyl nonadecanoate are the leab compounds where as complex compounds as (22e) \u2013ergost-6, 22(23), 24(28)- triene -5a, 5a-epidioxy-3\/3-o1 (3) and ergosterol (4) are the extra compounds present in sponge Suberites\u00a0carnosus.<\/em> 16<\/sup> have identified a major parasite class of sponge called d\u0435mospongia\u0435 is well-known for generating the diverse and biggest number secondary metabolites amongst the marine invertebrates. Many sponges derived bioactive compounds as ara-a (anti-viral), ara-c (anti-\u00a0cancer) and manoalide (phospholipids a 2 inhibitor), IPL512602 (anti-inflammatory), KRN 7000 (anticancer), laf 389(anticancer), discodemolide (anticancer), and HTI286 (anticancer) are now is being in clinical trials17,18<\/sup>. The study carried out by 19<\/sup> found the antinocic\u0435ptiv\u0435 and anti-inflammatory compounds from marine sponge Caissera.\u00a0 The structurally promising secondary metabolites of different genus of sponge Haliclona have been derived by20<\/sup>. The important pharmaceutical compounds have been identified by 2<\/sup>1,<\/sup> 22<\/sup> and they have identified the inhibition\u00a0of HIV by two bis-quinolizidine alkaloids petrosins from Indian sponge Petrosia similis<\/em> which inhibited HIV-1 replication. The study carried out by 23<\/sup> , reported the gorgosterol type\u00a0 sterol lacking methyl substitution of the cyclopropane ring system, namely 22,23-methylene (22S, 23S)\u00a0cholesterol 3 along with known compounds have been identified from marine sponge S. fibulata<\/em>. The previous work have also been carried out on same sponge by 24,25 <\/sup>determined the mixture of steroids and peptides for the first time.<\/p>\n

Characterization of isolated extracts of Sponge by HPTLC<\/span><\/strong><\/p>\n

The extracts isolated from the S.<\/em> fibulata (Schmidt)<\/em>collected from Nariman Point Mumbai coast are spotted on HPTLC plates and the plates were developed in a twin-trough chamber using I – butanol: methanol: water in the proportion of 3:1:1 (v\/v), as a mobile phase. The plates\u00a0were dried and sprayed with the dragendroff reagent .The photograph of the HPTLC plate of S.<\/em> fibulata (Schmidt)<\/em> and is shown in Photograph No.1, and 2. The spraying reagents gave positive tests for the presence of amides. The distinct and well-separated three spots were at Rf values of\u00a0S. fibulata <\/em>are G1- 0.029; G2- 0.137and G3-0.773. The densitometric scanning of these spots resulted in the quantification of these substances are found to be S.<\/em> fibulata <\/em>(67.69 %); (2.64 %); and (29.67 %) respectively. The pure compounds were isolated by scrapping the spots into\u00a0methanol. Pure compounds were obtained by evaporating the solvent methanol by vacuum desiccators. These compounds are then characterized by FTIR analysis.<\/p>\n\n\n\n
\u00a0\"Vol14No3_Iso_Bha_pho1\"<\/a><\/td>\nPhotograph 1: TLC of crude extract of Sponge S. fibulata<\/em><\/strong><\/p>\n

Click here to view\u00a0Photograph<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n
\"Vol14No3_Iso_Bha_pho2\"<\/a><\/td>\nPhotograph 2: Graph showing isolated compounds of Sponge S. fibulata<\/em><\/strong><\/p>\n

Click here to view\u00a0 Photograph<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Characterization of isolated extracts of sponge on GC-MS<\/strong><\/p>\n

GC-MS of the extracts isolated from the S.<\/em> fibulata <\/em>have been performed and the results are presented in Fig. No.3. the gas chromatograms of the extracts of the S.<\/em> fibulata <\/em>shown in Fig No. 3 G1 to G3, indicate that there are a large number of peaks. However the peaks at Rt value\u00a0of S.<\/em> fibulata <\/em>are 28.18; 28.20 and 28.21 were found suitable for identification. The other peaks could not be identified due to the lack of database in the library as well as any references that are reported till now for the S.<\/em> fibulata. <\/em>The GC peaks obtained at Rt value of S.<\/em> fibulata (Schmidt)<\/em>\u00a0were only employed for recording the mass spectra by irradiating the eluents at these Rt values through electron impact (EI+<\/sup>) source of the Mass spectrometer. Fig. No. 4 to 6, is the mass spectra of eluted compounds at Rt values 28.18; 28.20; 28.21; of S.<\/em> fibulata (Schmidt)<\/em>. The M\/Z\u00a0mass peaks obtained for eluents at Rt values 28.18; 28.20; 28.21; corresponds to the substances of molecular mass of isolated compounds of S.<\/em> fibulata (Schmidt)<\/em> are466, <\/em>330, and 374respectively. It is observed that the mass spectra obtained for S.<\/em> fibulata (Schmidt)\u00a0<\/em>the\u00a0compounds are found to be different indicating that the different substances (compounds) are present in all isolated compounds of S.<\/em> fibulata (Schmidt)<\/em>.<\/strong>The fragmentation peaks obtained for each of the mass spectra are as follows: For Rt value of compound 1 of S. fibulata<\/em> at 28.18 ;\u00a0\u00a0M\/Z at 466 (20%), M\/Z at 365(10%), M\/Z at 199 (27%), M\/Z at 143 (38%), M\/Z at 87 (82%), M\/Z at 74 (100%), and M\/Z at 57 (50%). For Rt value of compound 2 of S. fibulata<\/em> at 28.20; M\/Z at 330 (7%), M\/Z at 299(12% ), M\/Z at 239 (78%), M\/Z at 134 (60%), M\/Z at 98 (67%),\u00a0M\/Z at 74 (62%), and M\/Z at 43 (100%) and for Rt value of compound 3 of S. fibulata<\/em> at 28.21; M\/Z at 374 (11%), M\/Z at 267(8%), M\/Z at 155 (37%), M\/Z at 137 (14%), M\/Z at 71 (78%),M\/Z at 57 (96%), and M\/Z at 43 (100%) The gas chromatograms shown in Fig No.\u00a01,indicates that a large number of components are present in each of the isolated extracts S.<\/em> fibulata (Schmidt)<\/em>. However, for the present study we have selected the components of GC peaks at Rt values of S.<\/em>fibulata (Schmidt)<\/em> are 28.18; 28.20 and 28.21 only, because they gave some\u00a0conclusive evidence to identify the nature of the components on the database library. Therefore, the respective eluents at these Rt values were irradicated into the Electron impact (EI+<\/sup>) source of the mass spectrometer by which the molecules loose one electron and form positively charged\u00a0molecules.\u00a0 Thus the molecules separate in ion trap mass spectrometer. The MS spectra shown in FigNo, 4 to 6, indicate that the mass peaks are due to the compounds having molecular weight of S. fibulata<\/em> is 466, 330, and 374 respectively. These mass spectra are correlate to the Compounds\u00a0as Triacontanoic acid, methyl ester \u2013 (Skin irritant), Hexadecanoic acid, 2- hydroxyl- (hydroxymethyl) ethyl ester \u2013 (Fatty acid, Metabolite and Irritant) and 2-Nitro-1, 3-bis-oclyoxy-benzene, (A natural product found in Neolitsea daibuensis. It has a role as a plant metabolite and\u00a0an algal metabolite) of S. fibulata<\/em>. The structures of these molecules are as shown in Table No. 4.<\/p>\n\n\n\n
\"Vol14No3_Iso_Bha_fig3\"<\/td>\nFigure<\/strong> 3: Gas Chromatogram of the Isolated compound G1 to G3, of <\/strong>S. fibulata.<\/em><\/strong><\/p>\n

Click here to view figure\u00a0<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n
\"Vol14No3_Iso_Bha_fig4\"<\/a><\/td>\nFigure 4: <\/strong>Compound G1<\/strong><\/p>\n

Click here to view figure\u00a0<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n
\"Vol14No3_Iso_Bha_fig5\"<\/a><\/td>\nFigure 5: <\/strong>Compound G2<\/strong><\/p>\n

Click here toi view figure\u00a0<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n
\"Vol14No3_Iso_Bha_fig6\"<\/a><\/td>\nFigure 6: <\/strong>Compound G3.<\/strong><\/p>\n

Click here to view figure\u00a0<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

FTIR (Fourier Transform Infrared Spectroscopy) analysis<\/strong><\/p>\n

The FTIR studies are carried out in KBr pallets. Each of the compounds isolated at Rf values at G1- 0.029; G2- 0.137and G3-0.773 on TLC for each of the isolated compounds of S.<\/em> fibulata <\/em>extracts gave the IR spectras as shown in Fig No 7 to 9.All the IR spectra\u2019s of the\u00a0 compounds at the respective Rf values mentioned above are found to be dissimilar and different of the extracts isolated from S.<\/em> fibulata. <\/em>The wave numbers of some of the important IR peaks along with their intensity, type of vibration and probable groups present in the respective\u00a0compounds isolated at Rf values G1- 0.029; G2- 0.137and G3-0.773 are as shown in table.1 to 3.<\/p>\n\n\n\n
\"Vol14No3_Iso_Bha_fig7\"<\/a><\/td>\nFigure 7: <\/strong>FTIR spectra of isolated compound 1 of S. fibulata<\/em> at Rf values G1- 0.029.<\/strong><\/p>\n

Click here to view figure\u00a0<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n
\"Vol14No3_Iso_Bha_fig8\"<\/a><\/td>\nFigure 8: <\/strong>FTIR spectra of isolated compound 2 of Sigmadocia fibulata<\/em> at Rf values G2- 0.137.<\/strong><\/p>\n

Click here to view figure\u00a0<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n
\"Vol14No3_Iso_Bha_fig9\"<\/a><\/td>\nFigure<\/strong> 9: <\/strong>FTIR spectra of isolated compound 3 of S. fibulata<\/em> at Rf values G3-0.773.<\/strong><\/p>\n

Click here to view figure\u00a0<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Table 1: Correlation of IR spectra of the compound isolated from S. fibulata<\/em>\u00a0 at\u00a0 Rf value 0. 029 on TLC<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Sr.No.<\/strong><\/td>\nWave number: cm-1 <\/sup><\/strong>intensity<\/strong><\/td>\nType of Ir vibrations<\/strong><\/td>\nProbable group Assignment<\/strong><\/td>\n<\/tr>\n
1<\/td>\n3826.10<\/td>\nN-H- streatching vibration<\/td>\nO-H, N-H group<\/td>\n<\/tr>\n
2<\/td>\n3750.62<\/td>\nN-H- streatching vibration.<\/td>\nO-H, N-H group<\/td>\n<\/tr>\n
3<\/td>\n3445.73<\/td>\nN-H -streatching vibration.<\/td>\nO-H, N-H group<\/td>\n<\/tr>\n
4<\/td>\n2956.73<\/td>\nN-H -streatching vibration.<\/td>\nO-H, N-H group<\/td>\n<\/tr>\n
5<\/td>\n2920.15<\/td>\nN-H -streatching vibration.<\/td>\nO-H, N-H group<\/td>\n<\/tr>\n
6<\/td>\n2850.82<\/td>\nN-H -streatching vibration.<\/td>\nO-H, N-H group<\/td>\n<\/tr>\n
7<\/td>\n1739.03<\/td>\nC=N -streatching<\/td>\nC=0,C=C, C=N group<\/td>\n<\/tr>\n
8<\/td>\n1632.18<\/td>\nC=N- streatching<\/td>\nC=0,C=C, C=N group<\/td>\n<\/tr>\n
9<\/td>\n1464.50<\/td>\nC=N- streatching<\/td>\nC=0,C=C, C=N group<\/td>\n<\/tr>\n
10<\/td>\n1383.22<\/td>\nC=N -streatching<\/td>\nC=0,C=C, C=N group<\/td>\n<\/tr>\n
11<\/td>\n1217.97<\/td>\nC-O – stretching<\/td>\nC-O group<\/td>\n<\/tr>\n
12<\/td>\n1119.42<\/td>\nC-N stretching<\/td>\nC-N group<\/td>\n<\/tr>\n
13<\/td>\n1022.33<\/td>\n–<\/td>\nC-C group<\/td>\n<\/tr>\n
14<\/td>\n924.02<\/td>\n–<\/td>\nC-C group<\/td>\n<\/tr>\n
15<\/td>\n861.17<\/td>\nC-H bending<\/td>\nRock<\/td>\n<\/tr>\n
16<\/td>\n418.68<\/td>\n–<\/td>\nRock<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Table 2: Correlation of IR spectra of the compound isolated from S. fibulata<\/em>\u00a0 at\u00a0 Rf value 0.137on TLC<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Sr.No.<\/strong><\/td>\nWave number: cm-1 <\/sup>intensity<\/strong><\/td>\nType of Ir vibrations<\/strong><\/td>\nProbable group Assignment<\/strong><\/td>\n<\/tr>\n
1<\/td>\n3434.32<\/td>\nN-H stretching<\/td>\nO-H aliphatic primary amine<\/td>\n<\/tr>\n
2<\/td>\n2919.55<\/td>\nC-H stretching<\/td>\nC-H Alkyl stretch<\/td>\n<\/tr>\n
3<\/td>\n2849.99<\/td>\nC-H stretching<\/td>\nC-H group<\/td>\n<\/tr>\n
4<\/td>\n1736.87<\/td>\nC=O stretching<\/td>\nC=O group<\/td>\n<\/tr>\n
5<\/td>\n1631.94<\/td>\nC=C stretching<\/td>\nC=C alkene group<\/td>\n<\/tr>\n
6<\/td>\n1466.40<\/td>\nC-H bending<\/td>\nC=N group<\/td>\n<\/tr>\n
7<\/td>\n1413.15<\/td>\nC-H bending<\/td>\nC=N group<\/td>\n<\/tr>\n
8<\/td>\n1388.86<\/td>\nC-O<\/td>\nC-O group<\/td>\n<\/tr>\n
9<\/td>\n1312.04<\/td>\nC-O<\/td>\nC-O group<\/td>\n<\/tr>\n
10<\/td>\n1259.82<\/td>\nC-O stretching<\/td>\nC-O alkyl aryl ether group<\/td>\n<\/tr>\n
11<\/td>\n1119.07<\/td>\nC-O stretching<\/td>\nC-C group<\/td>\n<\/tr>\n
12<\/td>\n1023.97<\/td>\nC-C<\/td>\nC-C group<\/td>\n<\/tr>\n
13<\/td>\n921.70<\/td>\nC-C<\/td>\nC-C group<\/td>\n<\/tr>\n
14<\/td>\n862.66<\/td>\nC-C<\/td>\nC-C group<\/td>\n<\/tr>\n
15<\/td>\n15<\/td>\n<\/td>\nRock<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Table 3: Correlation of IR spectra of the compound isolated from S. fibulata<\/em>\u00a0 at\u00a0 Rf value 0.773on TLC<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n
Sr.No.<\/strong><\/td>\nWave number: cm-1 intensity<\/strong><\/td>\nType of Ir vibrations<\/strong><\/td>\nProbable group Assignment<\/strong><\/td>\n<\/tr>\n
1<\/td>\n1500-1600<\/td>\nC-C<\/td>\nO-H, N-H group<\/td>\n<\/tr>\n
2<\/td>\n1050-1150<\/td>\nN=O<\/td>\nO-H,N-H group<\/td>\n<\/tr>\n
3<\/td>\n2190-2140<\/td>\nC-O<\/td>\nC-H group<\/td>\n<\/tr>\n
4<\/td>\n1000-1350<\/td>\nC=C<\/td>\nC-H group<\/td>\n<\/tr>\n
5<\/td>\n2880-2900<\/td>\nC-N<\/td>\n<\/td>\n<\/tr>\n
6<\/td>\n3300<\/td>\nC-H<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n
\"Vol14No3_Iso_Bha_tab4\"<\/a><\/td>\nTable 4: Showing Name of the compound Molecular Weight, Molecular formula, and Structure of the compounds isolated from S. fibulata<\/em><\/strong>.<\/p>\n

Click here to view table<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Conclusion<\/strong><\/p>\n

From the above results it is concluded that, the compounds extracted from sponge S. fibulata <\/em>showed antibacterial, pesticidal, and biomedical properties as we have studied it well. Our findings are significant for the development of multi-drug therapy for both pharmaceuticals and\u00a0biomedical applications. Therefore we have screen the crude extracts of sponges for its structural elucidation to find the new drugs for pharmaceutical industry. The study further suggests that, Sigmadocia fibulata<\/em>further screening is required for molecular level to understand the\u00a0physiology and mode of action of the compound.\u00a0 The clinical study is also required which may be useful for pharmaceutical industry to manufacture the new drugs for safe performance and safety indexes to be studied to eradicate the diseases from mankind in future.<\/p>\n

Acknowledgement<\/strong><\/p>\n

Authors would like to thank to, Dr. P.A.Thomas, Principal Scientist, Central Marine Fisheries Research Institute (CMFRI), Thiruvananthapuram, Kerala for identification of sponge species. Authors are also thanks to, The Director, APT Testing & Research Pvt. Ltd. (ATR)\u00a0Pune, for experimentation and providing their assistance (IAEC NO. APTRF\/ RP 57\/1819 dated 01 February 2019).Thanks is also due to The Director, Ancrom test lab. Mulund, Mumbai, for providing HPTLC (High Performance Thin Layer Chromatography) facilities and also to the\u00a0Director, (SAIF) IIT, Powai, Mumbai for providing the facilities of analytical study.<\/p>\n

Conflict of Interest<\/strong><\/p>\n

There is no conflict of interest<\/p>\n

Funding Sources<\/strong><\/p>\n

None<\/p>\n

References<\/strong><\/p>\n

    \n
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