{"id":40373,"date":"2021-09-30T10:26:26","date_gmt":"2021-09-30T10:26:26","guid":{"rendered":"https:\/\/biomedpharmajournal.org\/?p=40373"},"modified":"2021-10-11T10:26:31","modified_gmt":"2021-10-11T10:26:31","slug":"the-effect-of-curcuminvs-curcumin-combined-antibiotic-ointmentson-the-healing-of-skin-wound","status":"publish","type":"post","link":"https:\/\/biomedpharmajournal.org\/staging\/vol14no3\/the-effect-of-curcuminvs-curcumin-combined-antibiotic-ointmentson-the-healing-of-skin-wound\/","title":{"rendered":"The effect of Curcuminvs. Curcumin Combined Antibiotic Ointmentson the Healing of skin wound"},"content":{"rendered":"

Introduction <\/strong><\/p>\n

Wounds are the main cause of physical illness,healing is a method of survival and an attempt to restore normal anatomical structure and function1\u00a0<\/sup>.<\/sub>Therefore,the property of living organisms is wound healing, in addition,wound cure is a process chain needed to extract invaded pathogens\u00a0from the wounded tissue of the body and to completely or partially restructure the injured tissue,in general, wound healing takes place in three complex and overlapping interrelated stages, namely inflammation, granulation, and remodeling2<\/sup>. Restoring damaged tissue is important for all surgical manipulations3<\/sup>. <\/sub>In general, wound healing is divided into three\u00a0interconnected complex and overlapping phases: inflammation, granulation, and remodeling,collagen formation occurs at a rapid rate in the early stages of wound healing, accompanied by collagen breakdown in the remodeling process, where collagen cross-linking\u00a0occurs and tensile strength increases,several factors influence the wound healing process, varying as age to nutritional status and neuropathy after wounding4<\/sup>. The cure of the wound is blocked by immune-suppressants, cytotoxins, and non-steroidal anti-inflammatory\u00a0drugs,antibiotic, analgesic, and herbal medications, however, improve wound healing5<\/sup>.<\/sub><\/p>\n

Various natural ingredients, plant ingredients that are composed of active principles such as triterpenes, alkaloids, flavonoids, and biomolecules facilitate the wound healing process\u00a04<\/sup>.<\/p>\n

Turmeric is an ancient spice of Curcuma longa rhizomes, which is in the genus of ginger (Zingiberaceae), for decades, turmeric has been used for medicinal purposes in India, Turmeric is one of the most effective healers of nature 6,7<\/sup>.Curcumin gives turmeric the yellow color and is\u00a0now known for most medical properties, 2-5 %of turmeric is estimated to be curcumin8<\/sup>.<\/p>\n

The characteristics of curcumin are antibiotics and antioxidants9<\/sup>.And speed up the re-epithelization of cells such as myofibroblast, fibroblast, and macrophages required for the cure of wounds\u00a010<\/sup>.<\/sub>Curcumin reduces the level of pain and inflammation and promotes homeostasis by\u00a0selectively inhibiting lipoxy and cyclooxygenase (COX) in the arachidonic acid cascadecombination 11<\/sup>.Turmeric is considered an easily available antiseptic for cuts, burns, and bruises in Ayurvedic medicine,turmeric is good antibiotic dermatology used in India, the\u00a0application of turmeric powder eliminates bacterial, fungal infections12<\/sup>.Combination antibiotic therapy is progressively used to lower the risk of antibiotic resistance in bacteria and to boost antibacterial range throughout therapy13<\/sup>.<\/sub>A few experiments have shown that antibacterial efficacy can be enhanced by combining antibiotics with crude plant extracts 14,15<\/sup>.<\/sub><\/p>\n

Skin wound healing is a fascinating process and not just for mammals, it is an evolutionary advantage, due to its essential functions as a physical, chemical, and bacterial barrier, skin wound healing is a significant step for wound survival, while certain interactions during the healing phase are essential, redundancy is high and other cells or mediators may assume roles\u00a0and signals without significant complications,in preserving the physiological homeostasis of the human body, the integrity of healthy skin is an important factor 16<\/sup>.<\/sub>There are many scoring or grading methods for evaluation of wound healing.\u00a0 One of these was used in a study that evaluate ethanolic extract of curcumin (curcuma Longa) on wound healing in black Bengal goats5<\/sup>, it\u00a0was precise, clear and simple represented way of\u00a0\u00a0 wound healing. For these reasons was chosen in this study. This scoring is divided to three grades:<\/p>\n

Grade I:\u00a0 presence of hemorrhage and debris<\/p>\n

Grade II: No debris, still there is hemorrhage with inflammatory cell infiltrate inside the tissue such as neutrophils, lymphocytes and macrophages.<\/p>\n

Grade III: decrease of inflammation, gap between the edges of the wound and then formation of keratin layer.<\/p>\n

Aim of this study: To evaluate the effects of Curcumin with and withoutantibiotics on skin wound treatment.<\/p>\n

Material and Methods<\/strong><\/p>\n

Animal modules and diagnostic samples<\/strong><\/p>\n

The protocol was approved by theanimal housein medical college \/ BasraUniversity. This study used 9 male rabbits aged about 6 months and an average weight of (1.083 g). They were kept in an air-conditioned house in stainless steel cages. This study was design to compare and evaluate\u00a0the effects of curcumin with and without antibiotic on healing of surgical wound in rabbits in three groups Each group consists of 3 rabbits:control that treated with normal saline group A, topical curcumin in group B, topical curcumin, and tetracycline ointment in group C. Regular\u00a0treatments were given to rabbits in therapeutic groups. The swelling of the suture area was evaluated at day 3 as the maximum of swelling achieved at the day 3, and the elevation of suture line was evaluated at day 7 which is the day at which skin suture was removed. Measurements of\u00a0swelling areas were recorded in millimeter (mm) by using slide calipers. T-test was used to evaluate the statistical differences. Skin samples of all rabbits were taken at day 3, 7and14of the injury. Skin biopsy was taken including the wound site and then fixed in formalin for histological\u00a0examination. After embedding in paraffin, slices were taken and stained by Hematoxylin and Eosin and Mallory Tricom for histological examination a modern Leica light microscope (1000X) magnification provided with a camera (0.5X), as well as a (10X) computerized\u00a0magnification which provided a high-resolution image.<\/p>\n

Turmeric paste preparation<\/strong><\/p>\n

Turmeric roots were collected from the nearby Iraqi market and then grinded by an electrical mill to a fine powder. The paste was prepared by water to (50g) of turmeric, water was gradually applied to the paste until it was semi-solid and then used on the injury directly.\u00a0Tetracycline Ointment: was bought from a pharmacy in Basra and used on the wound.<\/p>\n

Surgery procedure<\/strong><\/p>\n

Anesthetized Rabbits use an anesthetic (Lidocaine 2 %) solution to place the rabbit in a prone position and sterilize the lower back skin with a 10 % povidone-iodine solution. The cut was around 1 cm in the skin and sutured with 2-0 non-absorbed silk.<\/p>\n

Result and Discussion<\/strong><\/p>\n

The lowest Mean \u00b1 SD of swelling of suturing area ( at day 3) was noted in both groups that treated by curcumin alone (9.06 \u00b1 0.97 vs 15\u00b11 mm, p value = 0.002, table 1) and that treated with curcumine and antibiotic (9.1 \u00b10.9 vs 15\u00b11\u00a0 mm, p value =0.002, table 1)\u00a0 versus the control\u00a0group that treated by normal saline\u00a0 and the lowest Mean \u00b1 SD of elevation of suture line (at day 7) was noted in both group that treated by curcumin alone (2.63 \u00b1 0.06 vs 4.07\u00b10.21 mm, p value >0.001, table 1) and that treated with curcumin and antibiotic (2.7 \u00b1 0.2 vs 4.07\u00b10.21 mm, p\u00a0value =0.001, table 1), and this could indicate less inflammation in group B and C as compared with group A. This result could support the reports that describe the wound healing is a complex process depend on many cell types and many mediators [2]<\/sup> and the role of curcumine could be\u00a0through different mechanisms as will described below.Another interesting result was, no significant statistical difference between the Mean \u00b1 SD neither of swelling of suture area nor of elevation of suture lines of groups that treated by curcumin alone and group that treated with\u00a0curcumin and antibiotic [(9.06 \u00b1 0.97 vs 9.1 \u00b10.9, p value=0.97), (2.63 \u00b10.06 vs 2.7 \u00b1 0.2, p value=0.61) respectively, table 1].<\/p>\n

Table 1: represent Mean \u00b1 SD of the morphological difference (swelling of the suturing area and elevation of suture line) in three groups (A control group, B treated with curcumin and C treated with Curcumin and tetracycline Ointment).<\/strong><\/p>\n\n\n\n\n\n\n
Group<\/strong><\/td>\nMean<\/strong>\u00b1 <\/strong>SD of swelling of suturing area at Day 3 (mm)<\/strong><\/td>\nMean<\/strong>\u00b1<\/strong>SD of elevation of suture line at Day 7 (mm)<\/strong><\/td>\n<\/tr>\n
Group A<\/strong><\/td>\n15\u00b1 1<\/strong><\/td>\n4.06\u00b10.21<\/strong><\/td>\n<\/tr>\n
Group B<\/strong><\/td>\n9.06\u00b10.97<\/strong><\/td>\n2.63\u00b10.06<\/strong><\/td>\n<\/tr>\n
Group C<\/strong><\/td>\n9.1\u00b10.9<\/strong><\/td>\n2.70\u00b10.2<\/strong><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Another essential \u00a0result of this study, as shown in the (Table 2 and Figure 1) at day 3 of group A (control group), the grade of healing was between grade I and II because there is hemorrhage, inflammatory cell infiltrate mainly neutrophils, macrophages, lymphocytes and still there is debris while at day 3 of group B and C, the grade of healing was grade III (there is no hemorrhage and no debris and no inflammatory cell infiltrate and there is\u00a0 mature keratin layers formation (Figure 2) that indicate complete healing was achieved within 3 days in group B and C.<\/p>\n\n\n\n
\"Vol14No3_The_Ema_fig1\"<\/a><\/td>\nFigure 1: A-<\/strong>Representative section of day 3 of wound healing at group A (100X- Haematoxylin and eosin stain). There is debris, dilated blood vessels contain red blood cells with haemorrhage and slight inflammatory cell infiltrate. B- Representative section of day 3 wound healing at group A (400X-Haematoxylin and eosin stain). There is hemorrhage, red blood cells with slight inflammatory cell infiltrate.<\/strong><\/p>\n

Click here to view figure\u00a0<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n
\"Vol14No3_The_Ema_fig2\"<\/a><\/td>\nFigure 2: A<\/strong>-Representative section of day 3 of wound healing at group B (400X-Haematoxylin and eosin stain). There is full layer of keratin formation. B- Representative section of day 3 of wound healing at group B (40X-Haematoxylin and eosin stain). There is full layer of keratin formation.<\/strong><\/p>\n

Click here to view figure\u00a0<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Further more, at day 7 of group A (control group), the grade of healing of was between grade II and III because there is decrease of inflammation, presence of gap between the two edges of the wound but no keratin layer formation (Table 2 and Figure 3).\u00a0 This indicate complete healing\u00a0still was not achieved within 7 days after injury. While at day 7 of both group B and C, the grade of healing was grade III because mature keratin layer was formed (Figure 4).<\/p>\n\n\n\n
\"Vol14No3_The_Ema_fig3\"<\/a><\/td>\nFigure 3: A<\/strong>-Representative section of day 7 of wound healing at group A (40X-Haematoxylin and eosin stain). There is gab formation with slight inflammatory cell infiltration but no keratin formation. B-Representative section of day 7 wound healing at group A (400X-Haematoxylin and eosin stain). There is gab with slight inflammatory cell infiltration (lymphocytes, plasma cells and neutrophils) but no keratin formation<\/strong>.<\/p>\n

Click here to view figure<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n
\"Vol14No3_The_Ema_fig4\"<\/a><\/td>\nFigure 4: A<\/strong>-Representative section of day 7 of wound healing at group B (40X-Haematoxylin and eosin stain). There is full layer of keratin formation. B- Representative section of day 7 of wound healing at group B (400X-Haematoxylin and eosin stain). There is full layer of keratin formation.<\/strong><\/p>\n

Click here to view figure\u00a0<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Moreover, at day 14 of group A (control group), the grade of healing was grade III because there is mature keratin formation (Table 2 and Figure 5). While at both group (B and C), the process of healing was already reached the last stage of grade III at day 7 as there is formation of mature keratin (Table 2 and Figure 6).<\/p>\n

Table 2: Represent histological grade of wound healing in three groups that mentioned above.<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Group<\/strong><\/td>\nDay<\/strong><\/td>\nGrade of healing<\/strong><\/td>\n<\/tr>\n
Group A<\/strong><\/td>\n3<\/strong><\/td>\nGrade I to II<\/strong><\/td>\n<\/tr>\n
7<\/strong><\/td>\nGrade II to III<\/strong><\/td>\n<\/tr>\n
14<\/strong><\/td>\nGrade III<\/strong><\/td>\n<\/tr>\n
Group B<\/strong><\/td>\n3<\/strong><\/td>\nGrade III<\/strong><\/td>\n<\/tr>\n
7<\/strong><\/td>\nGrade III<\/strong><\/td>\n<\/tr>\n
14<\/strong><\/td>\nGrade III<\/strong><\/td>\n<\/tr>\n
Group C<\/strong><\/td>\n3<\/strong><\/td>\nGrade III<\/strong><\/td>\n<\/tr>\n
7<\/strong><\/td>\nGrade III<\/strong><\/td>\n<\/tr>\n
14<\/strong><\/td>\nGrade III<\/strong><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n
\"Vol14No3_The_Ema_fig5\"<\/a><\/td>\nFigure 5: <\/strong>Representative section of day 14 of wound healing at group A (100X-Mallory triple stain). There is full layer of keratin formation.<\/strong><\/p>\n

Click here to view figure\u00a0<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n
\"Vol14No3_The_Ema_fig6\"<\/a><\/td>\nFigure 6: A<\/strong> -Representative section of day 14 of wound healing at group B (100X- Mallory triple stain). There is full layer of keratin formation. B \u2013Representative\u00a0section of day 14 of wound healing at group C (100X-Mallory triple stain). There\u00a0\u00a0is full layer of keratin formation.<\/strong><\/p>\n

Click here to view figure<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

This indicate that the histopathological evaluation is consistent with morphological changes because there is hemorrhage, inflammatory cell infiltrate mainly neutrophils, macrophages, lymphocytes and still there is debris at day 3 of control group. Furthermore, at day 7 of control\u00a0group\u00a0\u00a0 there is decrease of inflammation, presence of gap between the two edges of the wound but no keratin formation. While at day 3 of both groups that treated by curcumin with and without antibiotic the wound healing was achieved and reach to thin layer of keratin at day 3 and\u00a0then to mature keratin at day 7. There is no clear histopathological difference in wound healing between tested groups that treated by curcumin with and without antibiotic. This indicate that curcumin alone (independent on adjuvant antibiotic) play a core role in the enhancement of\u00a0wound healing. As the wound healing is a complex process [2]<\/sup>. The role of curcumin in enhancement of wound healing is more complex 5<\/sup>.<\/p>\n

Many individual factors could affect healing such as age, neutritional factors, presence of neuropathy and others and as mention above\u00a04<\/sup>, but in the current study all these factors are standardized because all nine rabbits that submitted in this study have the same individual\u00a0features.Therefore, the effects of curcumin on wound healing could be due other mechanisms like anti-inflammatory, anti-bacteriological effect of curcumin9,11,12<\/sup>and antifungal13<\/sup>. The current study reveals an interesting finding that, the role of curcumine not only due to prevention\u00a0of factors that delay wound healing such as anti-inflammatory, antifungal and antibacterial effect, it also enhance wound healing through different mechanism such as enhancement of granulation tissue formation, contraction of the wound, enhancement tissue re-modeling ,\u00a0\u00a0releasing of chemotaxic agents, releasing of growth factors and re-epithelization\u00a0 of cells such as myofibroblast, fibroblast and macrophages required for the cure of the wound10<\/sup>. Therefore, might be all or more than one mechanism could be performed by curcumin. Although, some\u00a0microbilogical study, postulated the synergestic effect of some antibiotic with extract of some plants (curcumin was not used)15<\/sup>. One of an interesting finding of this study is that, there is no additive role of combination of tetracycline ointment with curcumin as compare with curcumine\u00a0alone on histopathological and morphological healing of the wounds. The result of this study was comparable to the result of other study as Sukandaret al<\/em>. (2016) postulated that combination of penicillin or ampicillin with curcumin could have synergestic effect against stapyllococusaureus, but this synergestic effect was not detected with tetracycline14<\/sup>.<\/p>\n

It is well known that combination of antibiotic therapy is progressively used to lower the risk of antibiotic resistance in bacteria and boost antibacterial range throughout therapy14,15<\/sup>. Therefore, the result of current study could indicate that curcumine could has more than one broad spectrum\u00a0anti-bacterial agents or could contain new anti-bacterial agent that didn\u2019t used before. Then the bacteria cannot resist the antibacterial effect.\u00a0 Therefore, the fundamental finding of this study is the curcumin alone (independent of adjuvant antibiotic) play a core role in the enhancement of wound healing.<\/p>\n

References <\/strong><\/p>\n

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    Introduction Wounds are the main cause of physical illness,healing is […]<\/p>\n","protected":false},"author":14,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[93],"tags":[],"class_list":["post-40373","post","type-post","status-publish","format-standard","hentry","category-vol14no3"],"_links":{"self":[{"href":"https:\/\/biomedpharmajournal.org\/staging\/wp-json\/wp\/v2\/posts\/40373","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/biomedpharmajournal.org\/staging\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biomedpharmajournal.org\/staging\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biomedpharmajournal.org\/staging\/wp-json\/wp\/v2\/users\/14"}],"replies":[{"embeddable":true,"href":"https:\/\/biomedpharmajournal.org\/staging\/wp-json\/wp\/v2\/comments?post=40373"}],"version-history":[{"count":5,"href":"https:\/\/biomedpharmajournal.org\/staging\/wp-json\/wp\/v2\/posts\/40373\/revisions"}],"predecessor-version":[{"id":41234,"href":"https:\/\/biomedpharmajournal.org\/staging\/wp-json\/wp\/v2\/posts\/40373\/revisions\/41234"}],"wp:attachment":[{"href":"https:\/\/biomedpharmajournal.org\/staging\/wp-json\/wp\/v2\/media?parent=40373"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biomedpharmajournal.org\/staging\/wp-json\/wp\/v2\/categories?post=40373"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biomedpharmajournal.org\/staging\/wp-json\/wp\/v2\/tags?post=40373"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}