{"id":3745,"date":"2015-12-28T11:14:36","date_gmt":"2015-12-28T11:14:36","guid":{"rendered":"http:\/\/biomedpharmajournal.org\/?p=3745"},"modified":"2020-04-25T03:41:56","modified_gmt":"2020-04-25T03:41:56","slug":"prevalence-of-reactive-oxygen-metabolites-levels-in-chronic-periodontitis-with-diabetic-mellitus-and-chronic-periodontitis-patients-in-gcf-plasma-and-saliva-a-biochemical-study","status":"publish","type":"post","link":"https:\/\/biomedpharmajournal.org\/staging\/vol8no2\/prevalence-of-reactive-oxygen-metabolites-levels-in-chronic-periodontitis-with-diabetic-mellitus-and-chronic-periodontitis-patients-in-gcf-plasma-and-saliva-a-biochemical-study\/","title":{"rendered":"Prevalence of Reactive Oxygen Metabolites Levels in Chronic Periodontitis with Diabetic Mellitus and Chronic Periodontitis Patients in GCF, Plasma and Saliva \u2013A Biochemical Study"},"content":{"rendered":"

Introduction <\/strong><\/p>\n

Periodontitis is a term used to describe an inflammatory process, initiated by the plaque biofilm, that lead to loss of periodontal attachment to the root surface and adjacent alveolar bone, and which ultimately results in tooth loss1<\/sup><\/strong>.The gram negative anaerobes are the primary etiologic agents2<\/sup><\/strong> but majority of breakdown occurs as a result of inappropriate host response .PMNS are the primary mediators of host response against pathogens and they generate increased levels of Reactive Oxygen Species(ROS)3<\/sup><\/strong>.<\/p>\n

ROS is a term collectively describing the hydroxyl(OH-)<\/sup>, hydroperoxyl (HOO-)<\/sup>, nitric oxide( NO-)<\/sup>, alkoxyl (Ro–<\/sup>), singlet oxygen(1<\/sup>O2<\/sub>), hypochlorous acid (HOCl) and hydrogen peroxide (H2<\/sub>O2<\/sub>)4<\/sup>. <\/strong>The changes in the host response as a consequence of various diseases can affect the severity and prognosis of periodontitis .One such disease is Diabetes Mellitus\u00a0 (DM),which encompasses a group of metabolic disorders characterized by hyperglycemia secondary to defects in insulin secretion, action (or) both. It has been reported that periodontitis is one of the first clinical manifestation of diabetes5<\/sup> <\/strong>and is recognised as a common complication in diabetic patients particularly in poorly controlled diabetes 6<\/sup><\/strong>. Diabetes Mellitus may contribute to the destructive aspects of the host response that leads to periodontal disease and other inflammatory conditions through the generation of reactive oxygen species (ROS)(7-10)<\/sup><\/strong>.Overproduction of ROS is also thought to\u00a0\u00a0 contribute to the severity of the disease,including vascular disease(11-12)<\/sup>.ROS are unstable , with a short half life , and thus are difficult to detect. Several markers of lipid peroxidation have been studied to monitor the \u00a0ROS production. Hydroperoxides are one of the most studied marker of ROS.<\/p>\n

In the present study ROM level is compared among subjects with Chronic periodontitis(CP) and Chronic periodontitis with Diabetes mellitus(CP-DM).<\/p>\n

Materials and Methods<\/strong><\/p>\n

The study population consisted of 40 subjects belonging to both sexes and all subjects were randomly selected from the outpatient clinic of the Department of Periodontics, Thaimoogambigai Dental college and Hospital, Chennai. Subjects were divided into two groups of 20 subjects each as with Group I- Chronic Periodontitis (CP)and Group II- Chronic Periodontitis with Diabetes\u00a0 Mellitus (CP-DM). The participants were informed about the study and their consent was obtained .The study protocol was approved by the ethical committee of Dr. M.G.R University,Chennai , India, in accordance with the Helsinki Declaration of 1975,as revised in 2000.<\/p>\n

Selection criteria<\/strong><\/p>\n

Inclusion criteria \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0\u00a0<\/strong><\/p>\n

Chronic Periodontitis<\/strong><\/p>\n