{"id":2809,"date":"2015-04-28T06:10:58","date_gmt":"2015-04-28T06:10:58","guid":{"rendered":"http:\/\/biomedpharmajournal.org\/?p=2809"},"modified":"2020-04-26T07:34:07","modified_gmt":"2020-04-26T07:34:07","slug":"present-status-future-road-map-and-diagnosis-of-zoonotic-mycotic-diseases","status":"publish","type":"post","link":"https:\/\/biomedpharmajournal.org\/staging\/vol6no2\/present-status-future-road-map-and-diagnosis-of-zoonotic-mycotic-diseases\/","title":{"rendered":"Present Status, Future Road Map and Diagnosis of Zoonotic Mycotic Diseases"},"content":{"rendered":"

Introduction<\/strong><\/p>\n

MYCOTIC diseases not only affect the animal health but also make the country\u2019s live stock Industry uneconomic, as they often lead to mastitis, decreased milk yield, abortions, sterility, deterioration of hide quality and above all diminished general performance.<\/p>\n

Due to prolonged and indiscriminate use of antibiotics and steroids in Intra-mammary therapy for mastitis, as well as prevalence of fungal organisms on dairy farms, the incidence of Mycotic Mastitis is on the rise and the most frequently isolated fungi from Mycotic Mastitis in dairy animals in India, are Cryptococcus neoformans and candida albicans.<\/p>\n

Systemic Mycotic diseases often pose a difficult diagnostic challenge for clinicians due to lack of any pathognomonic clinical syndrome and characteristic Radiographic feature. In India, the problem is further confounded by the preponderance of tuberculosis and paucity of diagnostic mycological laboratories. Consequently, patients suffering from systemic mycoses may be mistaken as Tuberculosis or other bacterial diseases.<\/p>\n

Among the emerging mycotic diseases in animals, documented in India, are ASPERGILLOSIS, ZYGOMYCOSIS, CRYPTOCOCCOSIS and CANDIDIASIS. Most of the reports of these diseases are based on the Isolation of fungi by microbiologists or Mycologists. In many cases, these fungi may be just contaminants, without being associated with the Pathological Lesion and the wrong diagnosis can be made.<\/p>\n\n\n\n
\"Figure<\/td>\n\n

Figure 1: Septate & dichotomously branching Aspergillus hyphae (GMS)<\/strong><\/p>\n

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Figure 2: Early Zygomycotic pyogranuloma in lung (HE + GMS)<\/strong><\/p>\n

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Figure 3: Cross section of fungal hyphae surrounded by splendor hoeppli bodies<\/strong><\/p>\n

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Figure 4: Hyphae, pseudohyphae & budding yeasts of Candida albicans in the mastitic milk smear (PAS)<\/strong><\/p>\n

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Figure 5:<\/strong> Yeast cells in dog lung tissue. PAS, 400X<\/strong><\/p>\n

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Figure 6: Sporangia showing different stages of sporangiospore development in the large sporangium, nasal polyp, PAS stain.<\/strong><\/p>\n

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Figure 7: Budding cryptococcus yeasts in the mastitic milk smear (PAS)<\/strong><\/p>\n

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Figure 8: Histoplasma capsulatum<\/strong><\/p>\n

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For diagnosis of mycotic diseases, demonstration of fungal material by special stains in the pathological lesion is a must. The correct and early diagnosis is a key factor in the treatment of mycotic diseases.<\/p>\n

Diagnosis of Mycotic Diseases<\/strong><\/p>\n

By the isolation of fungus in the culture.<\/p>\n

By histopathology using special stains like grocott\u2019s- methenamine silver, PAS & GRIDLEY Staining.<\/p>\n

By Immunohistochemistry with monoclonal antibodies, using indirect FAT and immunoperoxidase test.<\/p>\n

Recently sero-diagnosis to detect igG and igM have been used using DOT-ELISA<\/p>\n

Cutaneous hypersensitivity test has also been attempted by few workers.<\/p>\n

Genetic diagnosis including the use of PCR is still under Investigation, but this method suffers from low specificity and sensitivity.<\/p>\n

Diagnosis by X-rays, CAT-SCANS, ultrasound and MRI do not differentiate mycotic lesions from tuberculosis and cancerous growths.<\/p>\n

Accordingly, in most cases of mycotic infections, microscopic examination of biopsy or FNAC is the most reliable method of diagnosis even today. But for this purpose, the pathologist should have enough training because the morphology of the fungus and histopathology of the lesion varies according to the route of infection and at different stages of the disease, right from the onset of infection up to its termination.<\/p>\n

Therefore, for the benefit of young fresh myco-pathologists and post graduate students, I will now show some histopathological slides from some of the mycotic diseases.<\/p>\n

Conclusions<\/strong><\/p>\n

Respiratory and systemic mycoses are globally emerging as a problem of increasing importance in infectious diseases.\u00a0 This is attributed to the growing population of immunocompromised patients due to epidemic outbreak of AIDS or to other factors such as use of immunosuppressive drugs in recipients of organ transplantation.<\/p>\n

Since respiratory and systemic mycoses have no pathognomonic clinical or radiologic syndrome and mycological diagnostic facilities are restricted to only some of the major metropolitan centres, these diseases may be frequently confused with tuberculosis or other diseases of obscure etiology in India and other developing countries.<\/p>\n

Greater awareness and a high index of clinical suspicion are important pre-requisites for their diagnosis.\u00a0 Also, active collaboration of clinicians, pathologists, mycologists and microbiologists is advocated for their expeditious diagnosis and successful management, and in order to cope with the challenge of systemic mycoses, the health authorities of the developing countries are called upon to urgently take necessary measures for establishing a network of diagnostic medical and veterinary mycology laboratories.<\/p>\n

References<\/strong><\/p>\n

    \n
  1. Govindarajan, D. and Chauhan, R.S. (2001).\u00a0 Fungal diseases of cattle and buffaloes.\u00a0 Pashudhan.<\/em> 16 : 1 \u2013 8.<\/li>\n
  2. Gupta, P.P. and Mandal, P.C. (1999).\u00a0 Pathological alterations in goats intravenously infected with Aspergillus fumigates.<\/em> \u00a0Indian J. Anim. Sci. 69<\/em>: 381 \u2013 384.<\/li>\n
  3. Gupta, P.P., Singh, G., Sodhi, S. and Sood, N. (1999).\u00a0 Sequential clinical, haematological, biochemical, immunopathological and histopathological alterations in buffalo-calves (Bubalus bubalis<\/em>) intravenously infected with absidia corymbifera. Trop. Anim. Hlth. Prod. 31: <\/em>143 \u2013 160.<\/li>\n
  4. Gupta, P.P., Singh, G. and Sodhi, S. (1999).\u00a0 Haematological, biochemical and immunopathological studies in buffalo-calves intravenously infected with Absidia corymbifera.\u00a0 Indian J. Comp. Microbiol. Immunol. Inf. Dis. <\/em>30: 58 \u2013 61.<\/li>\n
  5. Kindo, A.J. (2010).\u00a0 PCR in diagnosis of fungal infections.\u00a0 8th<\/sup> National Conf.\u00a0 SIHAM, 4 \u2013 6 March, 2010 at A.I.I.M.S., New Delhi.<\/li>\n
  6. Kumar, R. and Thakur, S.D. (2003).\u00a0 Fungal diseases of large animals.\u00a0 Livestock \u2013 International. <\/em>7: 8 \u2013 10.<\/li>\n
  7. Mandal, P.C. and Gupta, P.P. (1993).\u00a0 Sequential pathological studies in goats infected intratracheally with Aspergillus fumigates.<\/em>\u00a0 Mycopathologia.<\/em>\u00a0 121: 77 \u2013 81.<\/li>\n
  8. Mandal, P.C. and Gupta, P.P. (1993).\u00a0 Experimental aspergillosis in goats : Clinical, haematological and mycological studies. J. Vet. Med. B. 40 :<\/em> 283 \u2013 286.<\/li>\n
  9. Mandal, P.C. and Gupta, P.P. (1994).\u00a0 Sequential pathological studies in the udder of goats intramammarily infected with Aspergillus fumigatus.<\/em>\u00a0 Mycopathologia.<\/em>\u00a0 126: 9 \u2013 14.<\/li>\n
  10. Randhawa, H.S. (2010).\u00a0 Is blastomycosis endemic in India.\u00a0 8th<\/sup> National Conf. SIHAM, 4 \u2013 6 March, 2010, A.I.I.M.S., New Delhi.<\/li>\n
  11. Randhawa, H.S. (2010).\u00a0 Histoplasmosis: An overview of its prevalence and diagnosis.\u00a0 8th<\/sup> National Conf. SIHAM, 4 \u2013 6 March, 2010, A.I.I.M.S., New Delhi.<\/li>\n
  12. Sestero, C.M. and Scalarone, G.M. (2006).\u00a0 Detection of IgG and IgM in sera from canines with blastomycosis.\u00a0 Mycopathologia. 162:<\/em> 33 \u2013 37.<\/li>\n
  13. Shastri, J. (2010).\u00a0 Rhinosporidiosis \u2013 a case report.\u00a0 8th<\/sup> National Conf. SIHAM, 4 \u2013 6 March, 2010, AIIMS, New Delhi.<\/li>\n
  14. Singh, G., Gupta, P.P., Sood, N., Banga, H.S. and Jand, S.K. (1998).\u00a0 Sequential pathological studies in Asian water buffaloes infected intratracheally with Absidia corymbifera.\u00a0 Rev. Iberoam. Micol. <\/em>15: 146 \u2013 150.<\/li>\n
  15. Singh, G., Gupta, P.P., Sood, N. and Banga, H.S. (1998).\u00a0 Pathological alterations in buffalo calves intravenously infected with Absidia corymbifera.\u00a0 Indian J. Anim. Sci… 68:<\/em> 1023 \u2013 1027.<\/li>\n
  16. Singh, M., Gupta, P.P., Rana, J.S. and Jand, S.K. (1994).\u00a0 Clinico-pathological studies on experimental cryptococcal mastitis in goats.\u00a0 Mycopathologia. <\/em>126: 147 \u2013 155.<\/li>\n
  17. Singh, M., Roy, K.S., Rana, J.S., Gupta, P.P. and Sood, N. (1995).\u00a0 Experimental cryptococcal mastitis in goats: histochemical studies.\u00a0 Indian J. Anim. Sci. <\/em>65: 742 \u2013 745.<\/li>\n
  18. Singh, M., Gupta, P.P., Rana, J.S., Sodhi, S. and Sood, N. (1994).\u00a0 Biochemical changes in milk in cryptococcal mastitis of experimental goats.\u00a0 Indian J. Dairy Sci. <\/em>47: 1043 \u2013 1049.<\/li>\n
  19. Singh, P., Sood, N., Gupta, P.P., Jand, S.K. and Banga, H.S. (1998).\u00a0 Experimental candidal mastitis in goats: clinical, haematological, biochemical and sequential pathological studies.\u00a0 Mycopathologia. <\/em>140: 89 \u2013 97.<\/li>\n
  20. Sondhi, J., Gupta, P.P. (2000).\u00a0 Effect of immunosuppression on the clinicopathological changes in experimental zygomycosis in rabbits.\u00a0 Vet. Res. Commun.<\/em> 24: 213 \u2013 227.<\/li>\n
  21. Sondhi, J., Gupta, P.P. and Sood, N. (1999).\u00a0 Effect of immunosuppression on the pathogenesis of respiratory zygomycosis in rabbits intranasally infected with Absidia corymbifera<\/em>.\u00a0 Rev. Iberoam. Micol. 16<\/em>: 107 \u2013 110.<\/li>\n
  22. Sondhi, J., Gupta, P.P., Sood, N. and Singh, J. (1999).\u00a0 Pathological and histochemical studies in experimental zygomycosis in rabbits.\u00a0 Indian J. Anim. Sci. <\/em>69: 694 \u2013 696.<\/li>\n
  23. Sondhi, J., Gupta, P.P. and Sood, N. (1999).\u00a0 Experimental zygomycosis in rabbits: Clinicopathological studies.\u00a0 Mycopathologia. <\/em>144: 29 \u2013 37.<\/li>\n
  24. Sood, N., Rana, J.S., Misri, J., Banga, H.S., Jand, S.K. and Gupta, P.P. (1996).\u00a0 Experimetnal cryptococcosis in buffalo calves.\u00a0 Buffalo J.<\/em> 12 : 139 \u2013 145.<\/li>\n
  25. Vidhya, P.S., Nagarajan, B., Manohar, B.M., Srinivasan, S.R. (2006).\u00a0 Histoplasma <\/em>colitis in a dog \u2013 a case report.\u00a0 Tamilnadu J. Vet. Anim. Sci.<\/em> 2: 151 \u2013 153.<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

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