{"id":26702,"date":"2019-03-25T10:02:04","date_gmt":"2019-03-25T10:02:04","guid":{"rendered":"http:\/\/biomedpharmajournal.org\/?p=26702"},"modified":"2020-04-23T10:54:43","modified_gmt":"2020-04-23T10:54:43","slug":"functional-features-of-platelet-secretion-in-piglets-during-early-ontogenesis","status":"publish","type":"post","link":"https:\/\/biomedpharmajournal.org\/staging\/vol12no1\/functional-features-of-platelet-secretion-in-piglets-during-early-ontogenesis\/","title":{"rendered":"Functional Features of Platelet Secretion in Piglets During Early Ontogenesis"},"content":{"rendered":"

Introduction<\/strong><\/p>\n

Optimum vitality of the body at any age by a range of mechanisms that support homeostasis.1,2<\/sup>\u00a0A huge role is played by the system of homeostasis, which continuously keeps the blood in the vessels in the liquid state3,4<\/sup>\u00a0and damage in various forms is strictly a local blood clot to stop the bleeding5<\/sup>. Balanced functioning of all components of hemostasis maintains blood volume and high viability of the organism.6,7<\/sup>\u00a0The hemostatic system largely determines the rheological characteristics of blood, primarily in the capillaries, adjusting the intensity of the trophic tissue in the body.8,9<\/sup><\/p>\n

The preservation of normal activity of hemostasis maintains the necessary level of viability of all productive animals throughout their development.10<\/sup>\u00a0It is therefore very important to conduct a detailed study of different aspects of the physiology of hemostasis. This information can seriously help in the intensification of their breeding and finding approaches to foster their economic implementation. In addition, this information can help in maintaining a functional optimum of their body under any adverse environmental conditions.11,12<\/sup><\/p>\n

Great functional importance in the whole system of hemostasis are thrombocytes. Manifestations of their hemostatic properties seriously determine the perfusion of small vessels, and, consequently, the intensity of metabolism in the tissues of the body.13<\/sup>\u00a0Very important for primary hemostasis can be considered secretory properties of platelets, because of their severity depends on the degree of involvement in the hemostatic process intact platelets and, consequently, the adequacy of primary hemostasis and optimum rheology of blood in the capillaries.14,15<\/sup>\u00a0In this regard, it is important to assess the secretory properties of platelets during growth and development of young productive animals, including pigs, being economically very important sources of meat in many countries.16<\/sup>\u00a0In this regard, the work the aim was to clarify the age dynamics of the secretory characteristics of platelets in piglets during early ontogenesis.<\/p>\n

Material and Methods<\/strong><\/p>\n

This study was carried out in full compliance with all ethical principles established in the decisions of the European Convention for the Protection of Vertebrates, used for experimental and other scientific purposes (adopted in Strasbourg on March 18, 1986 and confirmed in Strasbourg on June 15, 2006).\u00a0 It was approved by the local ethical committee of the Vologda State Academy of Dairy Farming.\u00a0 N. V. Vereshchagin (Minutes No. 12 of December 2, 2016), the Local Ethics Committee of the All-Russian Research Institute of Physiology, Biochemistry and Livestock (Minutes No. 11 of December 5, 2016) and the Local Ethics Committee of the Russian State Social University (Minutes \u211616 of December 7, 2016).<\/p>\n

The study was performed on piglets of optimal functional status of the large white breed: for 38 newborns, 35 dairy feeds, 37 dairy-plant feeds and 32 plant feeds.\u00a0 All animals taken in the study were obtained from completely healthy sows with 2-3 farrow.<\/p>\n

Taken from the piglets taken, blood was taken from the tail vein.\u00a0 Then, the traditional method was used to wash and resuspend platelets with an assessment of the concentrations of acyl hydroperoxides and malonic dialdehyde in them, using the thiobarbituric acid reduction reaction.17<\/sup><\/p>\n

The observed animals in intact and thrombin-activated platelets determined the content of actin and myosin, as well as the level of ADP and the severity of its secretion.18<\/sup>\u00a0 All obtained digital results of the study were processed by applying Student’s criterion.<\/p>\n

Results<\/strong><\/p>\n

During the first year of life in pigs, a gradual weakening of peroxidation processes in platelets was observed.\u00a0 This was indicated by a gradual decrease in the concentration in the platelets of piglets acylhydroperoxides (total 22.9%) and malondialdehyde (total 38.6%).<\/p>\n

The amount of actin in intact platelets in newborn piglets averaged 26.2 \u00b1 0.10% of the total protein in the platelet.\u00a0 At an older age, it gradually grew, reaching by the end of the first year of life 34.1 \u00b1 0.10% of the total protein in the platelet (Table 1).\u00a0 The intensity of additional education of actin on the background of platelet aggregation under the influence of a strong inducer during the first year of life in the observed piglets also gradually grew by a total of 17.8%.<\/p>\n

Table 1: Indicators of platelets in piglets during early ontogenesis.<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
Parameters<\/strong><\/td>\nNewborn phase,
\n<\/strong>n=38, M\u00b1m<\/strong><\/td>\n		Milk phase,
\nn=35, M\u00b1m<\/strong><\/td>\n		Milk and vegetable nutrition phase,
\n<\/strong>n<\/strong>=37, <\/strong>M<\/strong>\u00b1<\/strong>m<\/strong><\/td>\n		Phase of plant nutrition,
\n<\/strong>n<\/strong>=32, <\/strong>M<\/strong>\u00b1<\/strong>m<\/strong><\/td>\n<\/tr>\n
Acyl hydroperoxide platelets,<\/p>\n

D233 <\/sub>\/109<\/sup> platelets<\/td>\n

2.31\u00b10.011<\/td>\n2.17\u00b10.007<\/td>\n2.02\u00b10.011<\/p>\n

p<0.05<\/td>\n

1.88\u00b10.009<\/p>\n

\u0440<0.01<\/td>\n<\/tr>\n

Malonic dialdehyde platelets,<\/p>\n

nmol\/109<\/sup> platelets<\/td>\n

0.61\u00b10.011<\/td>\n0.57\u00b10.009<\/td>\n0.52\u00b10.011<\/p>\n

p<0.05<\/td>\n

0.44\u00b10.009<\/p>\n

\u0440<0.01<\/td>\n<\/tr>\n

The content of actin in intact platelets,% of the total protein content in the platelet<\/td>\n26.2\u00b10.10<\/td>\n27.5\u00b10.09<\/td>\n29.8\u00b10.12<\/p>\n

p<0.05<\/td>\n

34.1\u00b10.10<\/p>\n

\u0440<0.01<\/td>\n<\/tr>\n

The content of actin in platelets on the background of thrombin-aggregation,% of the total protein content in platelet<\/td>\n60.2\u00b10.11<\/td>\n61.5\u00b10.12<\/td>\n65.2\u00b10.13<\/p>\n

p<0.05<\/td>\n

70.9\u00b10.12<\/p>\n

\u0440<0.01<\/td>\n<\/tr>\n

The content of myosin in intact platelets,% of the total protein content in the platelet<\/td>\n\u00a010.9\u00b10.09<\/td>\n\u00a011.9\u00b10.09<\/td>\n\u00a014.5\u00b10.10<\/p>\n

p<0.05<\/td>\n

\u00a018.3\u00b10.09<\/p>\n

\u0440<0.01<\/td>\n<\/tr>\n

The content of myosin in platelets on the background of thrombin-aggregation,% of the total protein content in platelet<\/td>\n70.6\u00b10.08<\/td>\n72.4\u00b10.10<\/td>\n74.9\u00b10.14<\/p>\n

p<0.05<\/td>\n

79.5\u00b10.13<\/p>\n

\u0440<0.01<\/td>\n<\/tr>\n

The content of ADP in platelets, mmol\/109<\/sup> platelets<\/td>\n3.06\u00b10.07<\/td>\n3.23\u00b10.09<\/td>\n3.52\u00b10.07<\/p>\n

p<0.05<\/td>\n

3.96\u00b10.08<\/p>\n

\u0440<0.01<\/td>\n<\/tr>\n

The degree of secretion of ADP from platelets on the background of stimulation,%<\/td>\n32.9\u00b10.11<\/td>\n37.7\u00b10.09<\/td>\n43.0\u00b10.10<\/p>\n

p<0.05<\/td>\n

50.4\u00b10.15<\/p>\n

\u0440<0.01<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/p>\n

Legend: p – the reliability of the dynamics of the indicators taken into account relative to the level of the neonatal phase.<\/p>\n

In the first phase of ontogenesis, the level of myosin in piglets intact platelets was also low, accounting for 10.9 \u00b1 0.09% of the total protein content in the platelet, reaching 18.3\u00b10.09% of the total protein content of the platelet by 12 months.\u00a0 In the conditions of development of platelet aggregation in response to a strong inducer in piglets during all four phases of early ontogenesis, a gradual increase in this indicator in the amount of 12.6% was observed.<\/p>\n

In the blood plates of piglets, the content of ADP gradually increased during the first year of life. So, during the phase of colostrum nutrition, this indicator was the smallest in animals, then gradually increased in total by 29.4%. This was accompanied by their increased activity of its secretion (by 53.2%) in response to the stimulation of platelets by a strong inducer of their aggregation.<\/p>\n

Discussion<\/strong><\/p>\n

Given the high biological significance of the platelet hemostasis system and especially their secretory activity, there is still a high relevance for continuing the accumulation of knowledge in physiology.19<\/sup>\u00a0Of great importance for practice is the study of the ontogenetic dynamics of the secretory properties of platelets in productive animals and especially pigs.20<\/sup>\u00a0They are the most important strategic source of meat products in many countries of the world.\u00a0 In view of the fact that their main productive qualities are formed in early ontogenesis, it is of great interest to study the parameters of platelet secretion, which can affect the metabolism in various tissues.21,22<\/sup><\/p>\n

The level of secretory features of piglet platelets is largely ensured by the growth of the basal amount of actin and myosin in platelets and the intensification of self-assembly of their molecules under conditions of platelet aggregation in response to the inductor in the environment.23,24<\/sup><\/p>\n

The increased enhancement of platelet secretion in piglets of early ontogenesis, apparently, was caused by increased activity of the actin-myosin complex and an increase in the accumulation of adenosine phosphates in platelet granules.25<\/sup>\u00a0This process was facilitated by the weakening with age in piglets of the first year of life processes of lipid peroxidation in the structures of platelets.\u00a0 In addition, the peroxidation activity, which decreases as the age increases, apparently, provides for the accumulation in platelets of active G-proteins that transmit the signal from the receptor to the inside of the cell.26,27\u00a0<\/sup>The situation developing in the observed piglets contributed to an increase in the activity of a very functionally significant hemostatic mechanism of platelets – secretion.28,29<\/sup>\u00a0An increase in the activity of the secretory process in their platelets may indicate improvement in piglets of the mechanisms of primary hemostasis with age, which ensures a high degree of adaptation of their organism to the conditions of existence during the first year of life.30<\/sup><\/p>\n

Conclusion<\/strong><\/p>\n

In piglets during the first year of life, there is an increase in the secretory activity of their platelets.\u00a0 Important mechanisms that ensure its activation should be considered an increase in the number of actin and myosin in platelets with age and the intensification of their self-assembly under the influence of physiological stimulating effects on platelets.\u00a0 The growth of platelet secretion activity in piglets in early ontogenesis is also ensured by an increase in the number of ADP in their dense platelet granules and an increase in their release rate against the background of platelet activation by the inducer of the aggregation process.<\/p>\n

References<\/strong><\/p>\n

    \n
  1. Belozerova T. B and Agronina N. I. Independent Quality Estimation of Social Services: Rendering to Patients and Invalids. Prensa Med Argent.\u00a0<\/em>2017;103. 4doi: 10.4172\/lpma.1000255.
    \nCrossRef<\/a><\/li>\n
  2. Agronina N. I and Belozerova T. B. Rendering Social Services Assessment by Social Service Organizations in Belgorod and Kursk Regions in Russia. Prensa Med Argent.\u00a0<\/em>2017;103. 4 doi: 10.4172\/lpma.1000256.
    \n    CrossRef<\/a><\/li>\n
  3. Bikbulatova A. A and Andreeva E. G. Dynamics of Platelet Activity in 5-6-Year Old Children with Scoliosis Against the Background of Daily Medicinal-Prophylactic Clothes\u2019 Wearing for Half A Year. Biomed Pharmacol J.\u00a0<\/em>2017;10(3):16546. http:\/\/biomedpharmajournal.org\/?<\/li>\n
  4. Medvedev I. N. Vascular-platelet interaction in pregnant cows. J. Agric. Sci.\u00a0<\/em>2017;23(2):310-314.<\/li>\n
  5. Belozerova T. B and Agronina N. I. The Development of the Social Service System in Russia. Prensa Med Argent.\u00a0<\/em>2017;103:4\u00a0 doi: 10.4172\/lpma. 1000257.
    \n    CrossRef<\/a><\/li>\n
  6. Bikbulatova A. A. Dynamics of Locomotor Apparatus\u2019 Indices of Preschoolers with Scoliosis of I-II Degree Against the Background of Medicinal Physical Training. Biomed Pharmacol J.\u00a0<\/em>2017;10(3):16762. http:\/\/biomedpharmajournal.org\/?p=16762.<\/li>\n
  7. Sizov A. A. Investigation mistakes in investigation of crimes of foreign citizens, committed in the territory of Russia. Biosciences Biotechnology Research Asia.\u00a0<\/em>2015;12(1). http:\/\/www.biotech-asia.org\/?p=5452><\/li>\n
  8. Maksimov V. I., Parakhnevich A. V., Parakhnevich A. \u0410., Glagoleva T. I and Kutafina N. V. Rheological Properties of Erythrocytes of Healthy Piglets during the Transition from Dairy to Vegetable Nutrition. Annual Research & Review in Biology.\u00a0<\/em>2017;16(4):1-7. doi: 10.9734\/ARRB\/2017\/35865.
    \n    CrossRef<\/a><\/li>\n
  9. Maksimov V. I., Parakhnevich A. V., Parakhnevich A. \u0410., Glagoleva T. I and Kutafina N. V. Erythrocytes\u2019 Microrheological Features of Piglets during the Phase of Dairy-vegetable Nutrition after Damage or Common Supercooling. Annual Research & Review in Biology.\u00a0<\/em>2017;16(3):1-8. doi: 10.9734\/ARRB\/2017\/35864.
    \n    CrossRef<\/a><\/li>\n
  10. Maksimov V. I., Parakhnevich A.V., Parakhnevich A. \u0410., Glagoleva T. I and Kutafina N. V. Physiological Reaction of Erythrocytes\u2019 Micro Rheological Peculiarities in Milk Fed Piglets after the Negative Impact of the Environment. Annual Research & Review in Biology.\u00a0<\/em>2017;17(1):1-8. doi: 10.9734\/ARRB\/2017\/35867.
    \n    CrossRef<\/a><\/li>\n
  11. Maksimov V. I., Parakhnevich A. V., Parakhnevich A. \u0410., Glagoleva T. I and Kutafina N. V. Rheological Properties of Erythrocytes of Healthy Piglets during the Transition from Dairy to Vegetable Nutrition. Annual Research & Review in Biology.\u00a0<\/em>201;16(4):1-7\u00a0 doi: 10.9734\/ARRB\/2017\/35865.
    \n    CrossRef<\/a><\/li>\n
  12. Medvedev I. N. Correction of primary hemostasis in patients suffering from arterial hypertension with metabolic syndrome. Klinicheskaia meditsina.\u00a0<\/em>2007;85(3):29-33.<\/li>\n
  13. Medvedev I. N and Danilenko O. A. Complex correction of vascular hemostasis in patients with arterial hypertension, metabolic syndrome, and recent ocular vessel occlusion. Russian <\/em>Journal of Cardiology.\u00a0<\/em>2010;4:15-19.<\/li>\n
  14. \u00a0Ju L. O and Glagoleva T. I. Physiological Activity of Platelet Aggregation in Calves of Vegetable Feeding. Biomedical & Pharmacology Journal.\u00a0<\/em>2017;10(3):1395-1400.
    \n    CrossRef<\/a><\/li>\n
  15. Kutafina N. V. Platelet Parameters of Holstein Newborn Calves. Annual Research & Review in Biology.\u00a0<\/em>2017;15(2):1-8 doi: 10.9734\/ARRB\/2017\/35214.
    \n    CrossRef<\/a><\/li>\n
  16. Maksimov V. I., Parakhnevich A. V., Parakhnevich A. \u0410., Glagoleva T. I and Kutafina N. V. Erythrocytes\u2019 Microrheological Features of Piglets during the Phase of Dairy-vegetable Nutrition after Damage or Common Supercooling. Annual Research & Review in Biology.\u00a0<\/em>2017;16(3):1-8. doi: 10.9734\/ARRB\/2017\/35864.
    \n    CrossRef<\/a><\/li>\n
  17. Volchegorskij I. A., Dolgushin I. I., Kolesnikov O. L., Je V. C. Experimental modeling and laboratory assessment of adaptive reactions of the organism. Cheljabinsk. 2000:167.<\/li>\n
  18. Ermolaeva T. A. The program for the differential diagnosis of thrombocytopathy.\u00a0 Hematology and Transfusiology.\u00a0<\/em>1997;42(4):33-36.<\/li>\n
  19. Medvedev I. N. Platelet functional activity in clinically healthy elderly. Advances in gerontology.\u00a0<\/em>2016;29(4):633-638.<\/li>\n
  20. Maksimov V. I., Parakhnevich A.V., Parakhnevich A. \u0410., Glagoleva T. I and Kutafina N .V. Physiological Reaction of Erythrocytes\u2019 Micro Rheological Peculiarities in Milk Fed Piglets after the Negative Impact of the Environment. Annual Research & Review in Biology.\u00a0<\/em>2017;17(1):1-8. doi: 10.9734\/ARRB\/2017\/35867.
    \n    CrossRef<\/a><\/li>\n
  21. Vorobyeva N. V. Physiological Reaction of Erythrocytes\u2019 Microrheological Properties on Hypodynamia in Persons of the Second Mature Age. Annual Research & Review in Biology.\u00a0<\/em>2017;20(2):1-9.\u00a0 doi: 10.9734\/ARRB\/2017\/37718.
    \n    CrossRef<\/a><\/li>\n
  22. Medvedev I. N. Neuropsychologicalchanges in cognitive function during the transition of the elderly in old. Advances in gerontology.\u00a0<\/em>2015;28(3):479-483.<\/li>\n
  23. Bikbulatova A. A., Andreeva E. G and Medvedev I. N. Platelets\u2019 Functional Peculiarities in Persons of the Second Mature Age with Spinal Column Osteochondrosis of the Second Degree. Annual Research & Review in Biology.\u00a0<\/em>2017;21(1):1-9.\u00a0 doi: 10.9734\/ARRB\/2017\/37795.
    \n    CrossRef<\/a><\/li>\n
  24. Medvedev I. N. The Impact of Durable and Regular Training in Handto-hand Fighting Section on Aggregative Platelet Activity of Persons at the First Mature Age. Annual Research & Review in Biology.\u00a0<\/em>201;15(2):1-6.\u00a0 \u00a0doi: 10.9734\/ARRB\/2017\/35048.
    \n    CrossRef<\/a><\/li>\n
  25. Medvedev I. N. Physiological Dynamics of Platelets\u2019 Activity in Aged Rats. Annual Research & Review in Biology.<\/em> 2017;18(2):1-6. doi: 10.9734\/ARRB\/2017\/36821.
    \n    CrossRef<\/a><\/li>\n
  26. Mikhaylova I. V and Makhov A. S. Introducing chess education in Russian school system: Theoretical and practical aspects. Teoriya i Praktika Fizicheskoy Kultury.\u00a0<\/em>2018;5:53-55.<\/li>\n
  27. Medvedev I. N and Skoryatina I. A. Erythrocyte aggregation in patients with arterial hypertension and dyslipidemia treated with pravastatin. Klinicheskaia meditsina.\u00a0<\/em>2014;92(11):34-38.<\/li>\n
  28. Mikhaylova I. V., Makhov A. S., Seselkin A. I and\u00a0 Alifirov A. I. Chess in Russian inclusive education system: Problems and prospects. Teoriya i Praktika Fizicheskoy Kultury.\u00a0<\/em>2018;3:97-99.<\/li>\n
  29. Medvedev I. N and Skoryatina I. A. Pravastatin in correction of vessel wall antiplatelet control over the blood cells in patients with arterial hypertension and dyslipidemia. Cardiovascular therary and prevention.\u00a0<\/em>2014;13(6):18-22.
    \n    CrossRef<\/a><\/li>\n
  30. Ju L. O., Medvedev I. N and Fomina L.L. Platelets\u2019 Aggregative Properties of Ireshire Calves in the Phase of Dairy-vegetable Nutrition. Annual Research & Review in Biology.\u00a0<\/em>2017;16(4):1-6. doi: 10.9734\/ARRB\/2017\/35868.
    \n    CrossRef<\/a><\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

    Introduction Optimum vitality of the body at any age by […]<\/p>\n","protected":false},"author":9,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[63],"tags":[],"class_list":["post-26702","post","type-post","status-publish","format-standard","hentry","category-vol12no1"],"_links":{"self":[{"href":"https:\/\/biomedpharmajournal.org\/staging\/wp-json\/wp\/v2\/posts\/26702","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/biomedpharmajournal.org\/staging\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biomedpharmajournal.org\/staging\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biomedpharmajournal.org\/staging\/wp-json\/wp\/v2\/users\/9"}],"replies":[{"embeddable":true,"href":"https:\/\/biomedpharmajournal.org\/staging\/wp-json\/wp\/v2\/comments?post=26702"}],"version-history":[{"count":9,"href":"https:\/\/biomedpharmajournal.org\/staging\/wp-json\/wp\/v2\/posts\/26702\/revisions"}],"predecessor-version":[{"id":27038,"href":"https:\/\/biomedpharmajournal.org\/staging\/wp-json\/wp\/v2\/posts\/26702\/revisions\/27038"}],"wp:attachment":[{"href":"https:\/\/biomedpharmajournal.org\/staging\/wp-json\/wp\/v2\/media?parent=26702"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biomedpharmajournal.org\/staging\/wp-json\/wp\/v2\/categories?post=26702"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biomedpharmajournal.org\/staging\/wp-json\/wp\/v2\/tags?post=26702"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}