{"id":2628,"date":"2015-04-27T06:25:12","date_gmt":"2015-04-27T06:25:12","guid":{"rendered":"http:\/\/biomedpharmajournal.org\/?p=2628"},"modified":"2020-04-26T06:56:23","modified_gmt":"2020-04-26T06:56:23","slug":"synthesis-physico-chemical-spectral-and-hypoglycemic-activity-of-samarium-complex-of-glimepiride-an-oral-antidiabetic-drug","status":"publish","type":"post","link":"https:\/\/biomedpharmajournal.org\/staging\/vol6no1\/synthesis-physico-chemical-spectral-and-hypoglycemic-activity-of-samarium-complex-of-glimepiride-an-oral-antidiabetic-drug\/","title":{"rendered":"Synthesis, Physico-chemical, Spectral and Hypoglycemic Activity of Samarium Complex of Glimepiride, An Oral Antidiabetic Drug"},"content":{"rendered":"

Introduction <\/strong><\/p>\n

The study of chemistry and chemical reaction of co-ordination compound help in establishing structure activities relationship. It has been reported that in biological activity metal complex is more potent and less toxic as compared to the free ligand1-6. Inorganic chemistry and the use of metals in therapeutic drugs have become increasingly important over the last two of decades resulting in a variety of exciting and valuable drugs such as cis-platin for cancer.<\/p>\n

Recently metals in medicine has been recognised internationally as an important area for research. In this account the role of rare earth\u2019s metal neodymium has been undertaken for study7-10.<\/p>\n

In recent years, much attention is given to the use of suphonyl ureas because their high complexing nature with essential metals. Sulphonyl ureas are effective for non-insulin dependent diabetes mellitus11-13.<\/p>\n

These compounds are completely absorbed on oral administration. They are metabolized by liver and are excreted predominantly through urine. Complexation of sulphonyl ureas with rare earth\u2019s metals have been studied in detail by several workers14-16. A persual of available literatures shows that systemic study on complexation samarium with variours hypoglycemic drugs is relatively more important17-19.<\/p>\n

Here in the synthesis and characterization of samarium trioxide complex with glimepiride have been described20-22.<\/p>\n\n\n\n
\"Figure<\/td>\nFigure 1: Structure of Glimepiride.<\/strong><\/p>\n

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Ligand-metal ratio<\/strong><\/p>\n

For determining the ligand-metal ratio molar solutions were prepared of metal salt and ligand in 1:2 ratio and conductometric titrations were carried out by using monovariation method (Fig 2). The ligand-metal ratio (1:2) was also confirmed by the way of doing the jobs method23\u00a0of continuous variation using \u201d conductance. The indexed values were indicates 1:2 metal ligand ratio (Fig 3).<\/p>\n\n\n\n
\"Figure<\/td>\nFigure 2: Conductometric Titration graph of Samarium-Glimepiride Complex.<\/strong><\/p>\n

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\"Figure<\/td>\nFigure 3: Job\u2019s Method graph.<\/strong><\/p>\n

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Table 1:<\/strong> Glimepiride with samarium trioxide\u00a0<\/strong>(Job\u2019s Method)<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Glimepride \u2013 0.005 M<\/strong><\/td>\n\u00a0<\/strong><\/td>\n\u00a0<\/strong><\/td>\n\u00a0<\/strong><\/td>\nSm2O3\u2013 0.005 M<\/strong><\/td>\n\u00a0<\/strong><\/td>\n<\/tr>\n
Solvent \u2013 90% Ethanol<\/strong><\/td>\n\u00a0<\/strong><\/td>\n\u00a0<\/strong><\/td>\n\u00a0<\/strong><\/td>\nTemperature 31\u00b11\u00b0C<\/strong><\/td>\n\u00a0<\/strong><\/td>\n<\/tr>\n
Mole metal<\/strong><\/td>\n\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0Conductance<\/strong><\/td>\n\u00a0<\/strong><\/td>\nConductance<\/strong><\/td>\nCorrected-conductance<\/strong><\/td>\n\u00a0<\/strong><\/td>\n<\/tr>\n
ligand<\/strong><\/td>\n\u00a0<\/strong><\/td>\nX10-4Mhos<\/strong><\/td>\n\u00a0<\/strong><\/td>\nX10-4Mhos<\/strong><\/td>\n\u00a0X10-4Mhos<\/strong><\/td>\n<\/tr>\n
ratio<\/strong><\/td>\nM:S<\/strong><\/td>\nS:L<\/strong><\/td>\nM:L<\/strong><\/td>\nC1+C2-C3<\/strong><\/td>\n\u00a0<\/strong><\/td>\n<\/tr>\n
<\/td>\nC1<\/td>\nC2<\/td>\nC3<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
00:12<\/td>\n0.94<\/td>\n1.62<\/td>\n2.52<\/td>\n0.04<\/td>\n0<\/td>\n<\/tr>\n
01:11<\/td>\n1.57<\/td>\n1.55<\/td>\n2.96<\/td>\n0.16<\/td>\n0.12<\/td>\n<\/tr>\n
02:10<\/td>\n2.2<\/td>\n1.48<\/td>\n3.34<\/td>\n0.34<\/td>\n0.3<\/td>\n<\/tr>\n
03:09<\/td>\n2.8<\/td>\n1.46<\/td>\n3.73<\/td>\n0.53<\/td>\n0.49<\/td>\n<\/tr>\n
04:08<\/td>\n3.45<\/td>\n1.36<\/td>\n4.16<\/td>\n0.65<\/td>\n0.61<\/td>\n<\/tr>\n
05:07<\/td>\n3.95<\/td>\n1.15<\/td>\n4.66<\/td>\n0.44<\/td>\n0.4<\/td>\n<\/tr>\n
06:06<\/td>\n4.47<\/td>\n1.06<\/td>\n5.07<\/td>\n0.46<\/td>\n0.42<\/td>\n<\/tr>\n
07:05<\/td>\n4.58<\/td>\n0.89<\/td>\n5.29<\/td>\n0.28<\/td>\n0.24<\/td>\n<\/tr>\n
08:04<\/td>\n5.14<\/td>\n0.75<\/td>\n5.68<\/td>\n0.21<\/td>\n0.18<\/td>\n<\/tr>\n
09:03<\/td>\n5.13<\/td>\n0.71<\/td>\n5.69<\/td>\n0.15<\/td>\n0.11<\/td>\n<\/tr>\n
10:02<\/td>\n5.62<\/td>\n0.42<\/td>\n5.19<\/td>\n0.13<\/td>\n0.09<\/td>\n<\/tr>\n
11:01<\/td>\n6.21<\/td>\n0.32<\/td>\n6.45<\/td>\n0.08<\/td>\n0.04<\/td>\n<\/tr>\n
12:00<\/td>\n6.69<\/td>\n0.22<\/td>\n6.87<\/td>\n0.04<\/td>\n0<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

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Table 2:<\/strong> Glimepiride with samarium trioxide\u00a0<\/strong>(Job\u2019s Method)<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Glimepride \u2013 0.002 M<\/strong><\/td>\n\u00a0<\/strong><\/td>\n\u00a0<\/strong><\/td>\n\u00a0<\/strong><\/td>\nSm2O3\u2013 0.002 M<\/strong><\/td>\n\u00a0<\/strong><\/td>\n<\/tr>\n
Solvent \u2013 90% Ethanol<\/strong><\/td>\n\u00a0<\/strong><\/td>\n\u00a0<\/strong><\/td>\n\u00a0<\/strong><\/td>\nTemperature 31\u00b11\u00b0C<\/strong><\/td>\n\u00a0<\/strong><\/td>\n<\/tr>\n
Mole metal<\/strong><\/td>\n\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0Conductance<\/strong><\/td>\n\u00a0<\/strong><\/td>\nConductance<\/strong><\/td>\nCorrected-conductance<\/strong><\/td>\n\u00a0<\/strong><\/td>\n<\/tr>\n
ligand ratio<\/strong><\/td>\n\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0X10-4Mhos<\/strong><\/td>\n\u00a0<\/strong><\/td>\nX10-4Mhos<\/strong><\/td>\n\u00a0X10-4Mhos<\/strong><\/td>\n\u00a0<\/strong><\/td>\n<\/tr>\n
\u00a0<\/strong><\/td>\nM:S<\/strong><\/td>\nS:L<\/strong><\/td>\nM:L<\/strong><\/td>\nC1+C2-C3<\/strong><\/td>\n\u00a0<\/strong><\/td>\n<\/tr>\n
\u00a0<\/strong><\/td>\nC1<\/strong><\/td>\nC2<\/strong><\/td>\nC3<\/strong><\/td>\n\u00a0<\/strong><\/td>\n\u00a0<\/strong><\/td>\n<\/tr>\n
<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
00:12<\/td>\n0.93<\/td>\n1.66<\/td>\n2.51<\/td>\n0.08<\/td>\n0<\/td>\n<\/tr>\n
01:11<\/td>\n1.58<\/td>\n1.54<\/td>\n2.93<\/td>\n0.19<\/td>\n0.08<\/td>\n<\/tr>\n
02:10<\/td>\n2.21<\/td>\n1.44<\/td>\n3.32<\/td>\n0.33<\/td>\n0.27<\/td>\n<\/tr>\n
03:09<\/td>\n2.84<\/td>\n1.36<\/td>\n3.7<\/td>\n0.5<\/td>\n0.42<\/td>\n<\/tr>\n
04:08<\/td>\n3.47<\/td>\n1.25<\/td>\n4.18<\/td>\n0.54<\/td>\n0.48<\/td>\n<\/tr>\n
05:07<\/td>\n3.96<\/td>\n1.14<\/td>\n4.64<\/td>\n0.46<\/td>\n0.38<\/td>\n<\/tr>\n
06:06<\/td>\n4.48<\/td>\n1.06<\/td>\n5.12<\/td>\n0.42<\/td>\n0.34<\/td>\n<\/tr>\n
07:05<\/td>\n4.61<\/td>\n0.92<\/td>\n5.14<\/td>\n0.39<\/td>\n0.31<\/td>\n<\/tr>\n
08:04<\/td>\n5.02<\/td>\n0.79<\/td>\n5.44<\/td>\n0.37<\/td>\n0.29<\/td>\n<\/tr>\n
09:03<\/td>\n5.14<\/td>\n0.6<\/td>\n5.45<\/td>\n0.29<\/td>\n0.21<\/td>\n<\/tr>\n
10:02<\/td>\n5.62<\/td>\n0.46<\/td>\n5.89<\/td>\n0.19<\/td>\n0.09<\/td>\n<\/tr>\n
11:01<\/td>\n6.21<\/td>\n0.34<\/td>\n6.42<\/td>\n0.13<\/td>\n0.07<\/td>\n<\/tr>\n
12:00<\/td>\n6.69<\/td>\n0.21<\/td>\n6.84<\/td>\n0.06<\/td>\n0<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Experimental<\/strong><\/p>\n

Pure sample of glimepiride (trade name, amaryl) with m.f. (C24H34N4O5S) was received from Ipca laboratories Limited, Ratlam. Solvents and metal salts used were of the analytical grade. Melting point was determined by Parkin Elmer model melting point apparatus and are uncorrected, pH values determined on Lab. India pH analyser. IR spectra of ligand and complex were recorded with Perkin Elemer Spectrometer in the range of 4000-450 cm-1\u00a0(CDRI Lucknow). Nuclear magnetic spectral studies of ligand and complex were obtained from CDRI Lucknow (India).<\/p>\n\n\n\n
\"Figure<\/td>\nFigure 4(a) : IR Spectra of pure drug Glimepiride.<\/strong><\/p>\n

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\"Figure<\/td>\nFigure 4(b): IR Spectra of Glimepiride-Samarium complex.<\/strong><\/p>\n

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Table 3: Synthesis and physico chemical characteristics of Glimepiride-Samarium Complex.<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n
Ligand\/Complex<\/strong><\/td>\nLigand<\/strong><\/td>\nColour<\/strong><\/td>\n%yield<\/strong><\/td>\nStability<\/strong><\/td>\nFree energy<\/strong><\/td>\n<\/tr>\n
<\/td>\nMetal<\/td>\n<\/td>\n<\/td>\nconstant<\/td>\n\u00a0change<\/td>\n<\/tr>\n
<\/td>\nRatio<\/td>\n<\/td>\n<\/td>\nLogk<\/td>\n(-DF)<\/td>\n<\/tr>\n
<\/td>\n<\/td>\n<\/td>\n<\/td>\n(L\/mole)<\/td>\nKCal\/mole<\/td>\n<\/tr>\n
Glimepiride (Ref)<\/td>\n–<\/td>\nWhite<\/td>\n–<\/td>\n–<\/td>\n–<\/td>\n<\/tr>\n
Glimepiride-samarium<\/td>\n02:01<\/td>\nShiny<\/td>\n58<\/td>\n11.83<\/td>\n-16.26<\/td>\n<\/tr>\n
Complex<\/td>\n<\/td>\nGreyish<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n<\/td>\nCrystal<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Synthesis<\/strong><\/p>\n

A weighed quantity of glimepiride (2mol.) was dissolved in minimum quatity of 80% DMF. The samarium trioxide solution was prepared by dissolving it separately in the same solvent. A few drops of alkali NaOH solution was added to metal solution to increase the solubility. Metallic solution was added slowly with stirring into the solution of ligand at room temperature maintaining the pH between 6 to 8 by adding dilute NaOH solution and refluxed for 2-4 hours24-27\u00a0at 80\u00b0C. The solutions were left for crystallization at room temperature for 18-20 hours, shiny grey coloured crystals of complex were obtained which were filtered, washed, dried and then their melting points determined were recorded.<\/p>\n\n\n\n
\"Figure<\/td>\nFigure 5(a):<\/strong><\/p>\n

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\"Figure<\/td>\nFigure 5(b):<\/strong><\/p>\n

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Table 4: Analytical data of complex.<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n
Drug\/Complex<\/strong><\/td>\n<\/td>\nElemental analysis found (calculated)<\/strong><\/td>\n\u00a0<\/strong><\/td>\nM.P.<\/strong><\/td>\n\u00a0<\/strong><\/td>\n<\/td>\n<\/td>\n<\/tr>\n
\u00a0<\/strong><\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
\u00a0<\/strong><\/td>\nC<\/td>\nH<\/td>\nN<\/td>\nS<\/td>\nMetal<\/td>\nWater<\/td>\n\u00b0C<\/td>\n<\/tr>\n
C24H34N4O5S<\/strong><\/td>\n58.77<\/td>\n6.93<\/td>\n11.92<\/td>\n6.53<\/td>\n–<\/td>\n–<\/td>\n207<\/td>\n<\/tr>\n
\u00a0<\/strong><\/td>\n-58.8<\/td>\n-6.95<\/td>\n-11.94<\/td>\n-6.57<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
(C24H34N4O5S)2.Sm(OH2)4<\/strong><\/td>\n49.93<\/td>\n5.93<\/td>\n9.09<\/td>\n5.18<\/td>\n12.98<\/td>\n4.98<\/td>\n212<\/td>\n<\/tr>\n
<\/td>\n-50.9<\/td>\n-6.01<\/td>\n-9.89<\/td>\n-5.65<\/td>\n-13.25<\/td>\n-3.21<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

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Analysis of complex<\/strong><\/p>\n

The resulting complex so formed was characterized by its elemental analysis through SEM-EDAX method and IR, NMR studies (table 5 and 6) and metal was estimated by EDTA method.<\/p>\n

Table 5: IR Absorption data of the complex in cm<\/strong>-1.<\/strong><\/p>\n\n\n\n\n\n
Ligand\/Complex<\/strong><\/td>\nu (NH)<\/strong><\/td>\nu (C=O)<\/strong><\/td>\nu (S=O)<\/strong><\/td>\nu (C-O)<\/strong><\/td>\nu (C=N)<\/strong><\/td>\nu (SO2N<\/strong><\/td>\nu (M-O)<\/strong><\/td>\nu (H2O)<\/strong><\/td>\n<\/tr>\n
C24H34N4O5S<\/strong><\/td>\n3681<\/td>\n1706<\/td>\n1215<\/td>\n–<\/td>\n–<\/td>\n3020<\/td>\n–<\/td>\n–<\/td>\n<\/tr>\n
(C24H34N4O5S)2.Sm.4H2O<\/strong><\/td>\n3680<\/td>\n1702<\/td>\n1216<\/td>\n1648<\/td>\n2365<\/td>\n3021<\/td>\n670<\/td>\n3429<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

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Results and Discussion<\/strong><\/p>\n

Structure determination<\/strong><\/p>\n

IR Absorption Studies<\/strong><\/p>\n

The infrared spectrum of glimepiride and metal complex were recorded on Perkin Elemer Spectrometer RX1 (4000-450 cm-1). The major absorption bands for the infrared frequencies and the corresponding assignments are listed in
\nTable 5.<\/p>\n

Table 6: Some Important characteristics 1HNMR signals for glimepiride and its samarium complex.<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n
Glimepiride Pure<\/strong><\/td>\ndValue (ppm)<\/strong><\/td>\nAssignment<\/strong><\/td>\n<\/tr>\n
Ligand<\/td>\nGlimepiride-Samarium Complex<\/td>\n<\/td>\n<\/tr>\n
8.44(0.47 Hz)<\/td>\n7.94(1 Hz)<\/td>\n1HNMR signal for \u2013NHCO group and for<\/td>\n<\/tr>\n
<\/td>\n<\/td>\nAronatic proton)<\/td>\n<\/tr>\n
6.23(0.39 Hz)<\/td>\n–<\/td>\nNH proton signal for \u2013SO2NH group<\/td>\n<\/tr>\n
1.65(1.36 Hz)<\/td>\n1.56(0.64 Hz)<\/td>\n-CH2 proton signal<\/td>\n<\/tr>\n
1.04(2.82 Hz)<\/td>\n1.04(7.1 Hz)<\/td>\nMethyl (-CH3) proton signal<\/td>\n<\/tr>\n
–<\/td>\n3.78(1.11 Hz)<\/td>\nNHCO-M signal (M= metal)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

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The Glimepiride metal complex showed a prominent IR absorption band in the region 1706 cm-1\u00a0and 1702 cm-1\u00a0due to (C=O) carbonyl group28-29. The next IR band of structure significance of the ligand appears at 1215 cm-1\u00a0which may be assigned to (S=O) which got shifted at 1216 cm-1\u00a0in the complex. The NH group observed at 3681 cm-1\u00a0in the ligand (glimepiride) shifted to 3680 cm-1\u00a0in samarium glimepiride complex. The IR frequencies of (C=N) group was appeared at 2365 cm-1\u00a0in the complex while absent in ligand. The linkage through amide-O\u00a0 and sulphone-O- atom was further supported by the appearance of a band in the far IR region at 670 cm-1\u00a0in the complex that may be assignable to M-O frequency which was absent in ligand. The IR frequency region at 3429 cm-1\u00a0was assigned to nH2O molecule that may be not found in ligand frequency region. In pure ligand there is no absorption band detected for C-O and C=N due to enolisation are further supporting the structure for glimepiride samarium complex.<\/p>\n\n\n\n
\"Figure<\/td>\nFigure 6: Graphical Representation of Comparative Antidiabetic Activity Analysis\u00a0<\/strong>of Glimepiride-Samarium Complex by Oral Glucose Tolerance Test.<\/strong><\/p>\n

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NMR Spectral Analysis<\/strong><\/p>\n

The samarium complex of glimepiride [Fig V (b)] the chemical shift \u201d<\/strong>d value for NH in NHCO was observed at 7.94 ppm while the same –<\/strong>d value in pure ligand glimepiride was observed at 8.44 ppm30\u00a0showing a (0.50 ppm) down field shifting. The sulphonyl group in the complex is deshielded to a greater extent. This may be due to the sulphonyl group being adjacent to the bonding site and hence greater deshielding occurs in it31-35.<\/p>\n

Table 7: Hypoglycemic Activity by Oral Glucose Tolerance Test.<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Group<\/strong><\/td>\n\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Treatment<\/strong><\/td>\n\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Blood Glucose (mg\/dL)<\/strong><\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n(mg\/kg body weight)<\/td>\nFasting<\/td>\n30 min.<\/td>\n90 min.<\/td>\n<\/tr>\n
<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
I<\/td>\nControl group<\/td>\n73.8\u00b12.5884<\/td>\n142.8\u00b12.8635<\/td>\n120.6\u00b12.4083<\/td>\n<\/tr>\n
<\/td>\n+ Glucose (2g)<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n+ Vehicle<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
II<\/td>\nSamarium complex of<\/td>\n72.6\u00b12.0736<\/td>\n103.8\u00b12.3874*<\/td>\n84.8\u00b13.4928**<\/td>\n<\/tr>\n
<\/td>\nGlimepiride (2mg) +<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\nGlucose + Vehicle<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
III<\/td>\nPure drug Glimepiride<\/td>\n74.4\u00b12.8809<\/td>\n100.6\u00b12.7018*<\/td>\n90.2\u00b12.5884**<\/td>\n<\/tr>\n
<\/td>\n(2mg) + Glucose + Vehicle<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

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\"Figure<\/td>\nFigure 7: Structure of Glimepiride-Samarium Complex.<\/strong><\/p>\n

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Antidiabetic activity<\/strong><\/p>\n

The isolated glimepiride-metal complex were found to be more potent as compared to the parent drug. Hence as compare to standard synthetic drug the glimepiride-samarium complex36-37\u00a0is having more hypoglycemic activity. The hypoglycemic effect of glimepiride as well as metal complex were investigated on the blood sugar levels of male wistar rats by Oral glucose tolerance test38. (PBRI, lab. Bhopal, M.P., India). Analysis of data presented in table (7) reveals that the drug caused a marked decrease in blood sugar level. On comparing the hypoglycemic effect of samarium complex with parent drug it was revealed that in case of Sm- glimepiride treated male wistar rats blood sugar falls to 84.8 \u00b1 3.4928 mg\/dl while in glimepiride treated rats blood sugar level falls to 90.2 2.5884 mg\/dl. These results clearly indicate a better hypoglycemic activity of Sm-glimepiride complex over its parent drug.39-40<\/p>\n

Conclusion<\/strong><\/p>\n

From the monovariation studies conducted by conductivity method and further confirmsed by Job\u2019s method of continuous variation suggest the ligand-metal ratio as L2M. Analytical data agrees to the molecular formula (C24H34N4O5S)2.Sm.4H2O. The structure of the complex proposed on the basis of analytical data and stoichiometry was further supported by IR and NMR spectral studies which suggest the linking of sulphonyl and enolic oxygen to the metal atom. The disappearance of enolic hydrogen in complex as indicated by IR values. The results of NMR spectra are well in agreement with the mol.wt of the complex. Hypoglycemic activities of the complex shows more blood sugar lowering effect as compared to parent ligand. Calculating the toxicity in the complexes and on many trials on monkey\u2019s and men the complex may be introduced as medicine in future.<\/p>\n

Acknowledgements<\/strong><\/p>\n

The authors express their sincere and grateful thanks to principal, Sadhu Vaswani College Bairagarh for providing necessary lab facilities, and Quality Assurance Department of Ipca Laborateries Ltd. Ratlam for gift of Glimepiride and IR and NMR spectral analysis.<\/p>\n

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