{"id":21014,"date":"2018-06-25T10:10:31","date_gmt":"2018-06-25T10:10:31","guid":{"rendered":"http:\/\/biomedpharmajournal.org\/?p=21014"},"modified":"2020-04-23T09:16:13","modified_gmt":"2020-04-23T09:16:13","slug":"gc-ms-analysis-of-one-ayurvedic-preparation-aswagandharishtam","status":"publish","type":"post","link":"https:\/\/biomedpharmajournal.org\/staging\/vol11no2\/gc-ms-analysis-of-one-ayurvedic-preparation-aswagandharishtam\/","title":{"rendered":"GC MS Analysis of one Ayurvedic Preparation \u2018Aswagandharishtam\u2019"},"content":{"rendered":"

Introduction<\/strong><\/p>\n

Ashwagandharishta\u00a0or Aswagandharishtam is a liquid Ayurveda medicine used in the treatment of\u00a0feeling tired all the time, \u00a0psychiatric conditions, dullness, loss of memory, sluggishness, epilepsy, stomach problems\u00a0like low digestion power, emaciation, Piles and\u00a0Vata imbalance\u00a0diseases. This increases ojas, nourishes all dhatus. It is also used as a nervine tonic.
\nsexual disorders, depression etc.\u00a0Ashwagandharishta contains about 5 \u2013 10 % of self generated natural alcohol in it acts as a media to deliver water and alcohol soluble the active herbal components to the body. The dosage of this medicine in usually 12-24 ml twice daily after food or as advised by the physician. Not much scientific data of validation of this medicine is available. The present work undertakes the GC MS analysis of this medicine to throw some light on the type of molecules present in Ashwagandha and their medicinal role. This is a first step in the direction of understanding the medicinal efficacy of Aswagandharistam. This Arishtam is made of the following ingredients and the following paragraphs indicate the medicinal role of each of the ingredients briefly. The ingredients are divided into two sections: Kwatha dravyaas and Prakshepa dravyaas.<\/p>\n

The coarse powder of Kwatha dravyaas are added with water, boiled and reduced to 12.288 liters, filtered. It is added with honey and Prakshepa Dravyaa powders are added and kept in an air tight container for one month for fermentation. After a month time, it is filtered and preserved. The manufactures of this medicine are Baidyanath, Dabur, AVN, AVP, Vaidik Herbs and Kottakkal Arya Vaidya Sala.<\/p>\n

Ashwagandharishtam Ingredients<\/strong><\/p>\n

Ashwagandha (Withania somnifera<\/em>) \u00a0\u2013 Root \u2013 2.4 kg<\/p>\n

Mushali (Chlorophytum tuberosum<\/em>) \u2013 Root \u2013 960 g<\/p>\n

Manjishta (Rubia cordifolia<\/em>) \u2013 Root \u2013 480 g<\/p>\n

Haritaki (Terminalia chebula<\/em>) \u2013 Fruit \u2013 480 g<\/p>\n

Nisha \u2013 Turmeric \u2013 (Curcuma longa<\/em>) rhizome \u2013 480 g<\/p>\n

Daruharidra (Berberis aristata<\/em>) \u2013 Stem \u2013 480 g<\/p>\n

Yashtimadhu \u2013 Licorice \u2013 (Glycrrhiza glabra<\/em> )Root \u2013 480 g<\/p>\n

Rasna (Pluchea lanceolata<\/em>) \u2013 Root \/ leaf \u2013\u00a0480 g<\/strong><\/p>\n

Vidari (Pueraria tuberosa<\/em>) \u2013 Root \u2013\u00a0480 g<\/p>\n

Arjuna (Terminalia arjuna<\/em>) \u2013 stem bark \u2013\u00a0480 g<\/p>\n

Mustaka (Cyperus rotundus<\/em>) \u2013 Rhizome \u2013\u00a0480 g<\/p>\n

Trivrit (Ipomoea turpethum<\/em>) \u2013 Root \u2013\u00a0480 g<\/p>\n

Sariva (Indian sarsaparilla \u2013 Hemidesmus indicus<\/em>) \u2013 Root \u2013 384 g<\/p>\n

Krishna Sariva (Cryptolepis buchanani<\/em>) \u2013 Root \u2013\u00a0384 g<\/p>\n

Shweta Candana (Santalum album<\/em>) \u2013 heart wood \u2013 384 g<\/p>\n

Rakta Candana (Pterocarpus santalinus<\/em>) \u2013 heart wood \u2013\u00a0384 g<\/p>\n

Vacha (Acorus calamus<\/em>) \u2013 Rhizome \u2013\u00a0384 g<\/p>\n

Chitraka (Plumbago zeylanica<\/em>) \u2013 Root \u2013\u00a0384 g<\/p>\n

Water for decoction \u2013 98.304 liters<\/p>\n

Boiled and reduced to 12.288 liters.<\/p>\n

Madhu \u2013 Honey \u2013 14.4 kg<\/p>\n

rakshepa \u2013 Dravyas<\/p>\n

Dhataki –\u00a0Woodfordia fruticosa<\/em> \u2013 Flower \u2013 768 g<\/p>\n

Shunti \u2013 Ginger \u2013 (Zingiber officinalis<\/em>) Rhizome \u2013 96 g<\/p>\n

Maricha \u2013 Pepper \u2013 \u00a0(Piper nigrum<\/em>) fruit \u2013 96 g<\/p>\n

Pippali \u2013 Long pepper \u2013\u00a0(Piper longum<\/em>) – 96 g<\/p>\n

Twak \u2013 Cinnamon \u2013 (Cinnamomum zeylanicum)<\/em> – <\/em>192\u00a0g<\/p>\n

Ela \u2013 Cardamom \u2013 (Elettaria <\/em>cardamomum<\/em>) – 192\u00a0g<\/p>\n

Patra (Cinnamomum tamala<\/em>) \u2013 leaves \u2013 192\u00a0g<\/p>\n

Priyangu (Callicarpa macrophylla<\/em>) \u2013 Flower \u2013 192 g<\/p>\n

Nagakeshara \u00a0(Mesua ferrea<\/em>) \u2013 Stamen \u2013 96 g<\/p>\n

The medicinal properties of the ingrediants are mentioned here under briefly.<\/p>\n

Ashwagandha \u2013 Winter cherry\/ Indian Ginseng (root) \u2013Withania somnifera <\/em>(L.) Dunal.<\/strong><\/p>\n

This plant has medicinal values such as immuno-modulator, aphrodisiac, antitumor, anti-inflammatory, anti stress, antioxidant, sleep inducing, effective in memory related conditions,\u00a0 and cardiopulmonary systems (Uddin et al<\/em>, 2012).1<\/sup> The phyto-constituents present in this plant like Withanoside IV or VI produced dendritic outgrowth in normal cortical neurons of isolated rat cells, whereas axonal outgrowth was observed in the treatment with withanolide A in normal cortical neurons.2<\/sup> The crude extract of the plant containing the steroidal substances sitoinodosides VII\u2013X and withaferin A augmented learning acquisition and memory in both young and old rats.3<\/sup><\/p>\n

Mushali<\/strong><\/em> (<\/strong><\/em>Chlorophytum tuberosum<\/strong><\/em>\u00a0<\/strong>Baker)<\/strong><\/p>\n

The tuberous roots are medicinally important and are known commonly as\u00a0safed musali<\/em>.\u00a0Safed musali<\/em>\u00a0is used as an aphrodisiac and galactogogue as well as for its nutritive, health promoting properties and immune-enhancing, hepatoprotective and antioxidants activities.4-6<\/sup> \u00a0 The tubers are also used in fever and leucorrhoea.<\/p>\n

Bhandi (Rubia cordifolia)<\/em><\/strong><\/p>\n

This plant is reported to have medicinal properties like anti-acne, anti-inflammatory, antibacterial and antioxidant.7-12<\/sup><\/p>\n

Haritaki \u2013 Chebulic Myrobalan fruit rind \u2013 Terminalia chebula<\/em><\/strong><\/p>\n

\u00a0<\/em>One of the constituent of the common Triphala choornam, T. chebula <\/em>bark, rind, galls etc. have been found to have activities like antioxidant, antimicrobial, antidiabetic, hepato protective, anti-inflammatory and anti arthritic, anti mutagenic, anti proliferative, radio protective, cardio protective, hypo lipidemic, antispasmodic, Immuno-modulatory and antiviral activities.13<\/sup><\/p>\n

Nisha \u2013 Turmeric- Curcuma longa<\/em><\/strong><\/p>\n

Turmeric is another important medicinal plant with its wide application as food, medicine and as preservative. Many workers have worked on this plant on various aspects. Turmeric is anti-inflammatory, antimicrobial, preservative, antifungal, anticancer, cardio protective, hypoglycemic and antidiabetic.14<\/sup><\/p>\n

Daruharidra <\/strong>(Berberis aistata<\/em>)<\/strong><\/p>\n

Berberis aristata<\/em> is ethno botanically important herb that is used from time immemorial by mankind for the treatment of various ailments. Sharma et al.<\/em>, 2011 has reviewed this plant\u2019s therapeutic role such as hepato-protective, hypoglycemic, anticancer, antimicrobial, anti-inflammatory, antioxidant etc. among many other medicinal values.16<\/sup><\/p>\n

Yashti \u2013 Licorice \u2013 Glycrrhiza glabra<\/em><\/strong><\/p>\n

Glycrrhiza glabra <\/em>is known for its medicinal properties such as anti-tussive & expectorant, antioxidant and antibacterial, anti-inflammatory, antiviral, memory enhancer, antifungal,<\/p>\n

antibacterial, anti-hyperglycemic, immune-stimulatory, hepato-protective, anticancer and<\/p>\n

anticoagulant.17<\/sup><\/p>\n

Rasna (<\/em>Pluchea lanceolata)<\/em><\/strong><\/p>\n

This plant is used as antirhematic, antiarthritic and as anti-inflammatory.18<\/sup><\/p>\n

Vidari<\/strong> (Pueraria tuberose)<\/em><\/strong><\/p>\n

Various\u00a0in vitro<\/em>\u00a0experimental models earlier have established its anti-oxidant and anti-inflammatory property.19,20<\/sup> Some of its other documented biological properties are anti-hyperglycemic, anti-hyperlipidemic, anti-fertility in male rats, and hepatoprotective.22-25<\/sup> The tubers are rich in isoflavonoids and terpenes with daidzein, puerarin, puetubersoanol, and tuberosin as bioactive phytochemicals.26<\/sup><\/p>\n

Arjuna– Terminalia arjuna<\/em><\/strong><\/p>\n

Terminalia arjuna <\/em>is one of the most versatile medicinal plants having a wide spectrum of biological activity. The Hypocholesterolaemic effects of Terminalia arjuna <\/em>tree bark was reported by Ram et al<\/em>, 1997, in rabbits.27<\/sup> Antioxidant and hypocholesterolaemic effects of Terminalia arjuna <\/em>tree bark powder was reported by Gupta et al<\/em>, 2001.27<\/sup> \u00a0The bark of T. arjuna <\/em>is anti-dysenteric, antipyretic, astringent, cardiotonic, litho-triptic, anticoagulant, hypolipidemic, antimicrobial and antiuremic agent.29-31<\/sup> Many useful phytoconstituents have been isolated from T. arjuna <\/em>which included triterpenoids for cardiovascular properties, tannins and flavonoids for its anticancer, antimicrobial properties and so on.32<\/sup> In studies on mice, its leaves have been shown to have analgesic and anti-inflammatory properties.33<\/sup><\/p>\n

Mushtaka – Cyperus rotundus<\/em><\/strong><\/p>\n

According to the Ayurveda, C. rotundus<\/em> rhizomes are considered astringent, diaphoretic, diuretic, analgesic, antispasmodic, aromatic, carminative, antitussive, emmenagogue, litholytic, sedative, stimulant, stomachic, vermifuge, tonic and antibacterial. It is also useful for dietary management of psychotic diseases and metabolic disorders (Sivapalan, 2013)34<\/sup> They are used in treatment of nausea and vomiting, dyspepsia, colic, flatulence, diarrhoea, dysentery, intestinal parasites, fever, malaria, cough, bronchitis, renal and vesical calculi, urinary tenesmus, skin diseases, wounds, amenorrhoea, dysmenorrhoea, deficient lactation, loss of memory, insect bites, food poisoning, indigestion, nausea, dysuria, bronchitis, infertility, cervical cancer and menstrual disorders, and the aromatic oils are made of perfumes and splash.35,36<\/sup><\/p>\n

Trivrit \u2013 Operculina turpethum<\/em><\/strong><\/p>\n

Kohli et al<\/em>, 2010 have given an exhaustive review on the medicinal importance of Operculina turpethu<\/em>m.37<\/sup> This plant has activities such as antisecretory, ulcer protective and anti-inflammatory, hepatoprotective, antibacterial activity, antioxidant and cytotoxic.38,41<\/sup><\/p>\n

Shweta and Krishnasariva \u2013 Indian Sarsaparila \u2013 Hemidesmus indicus<\/em><\/strong><\/p>\n

This plant is a very rich medicinal resource having activities like antiarthritic, anticancerous, antimicrobial, antidiarrhial, anti-inflammatory, antioxidant, hepatoprotective, nootropic and antileprotic.41<\/sup><\/p>\n

Krishna Sariva – Cryptolepis buchanani<\/em><\/strong><\/p>\n

Hanprasertpong et al<\/em>, 2014, have reported that Cryptolepis buchanani<\/em>\u00a0Roem. & Schult. (Asclepiadaceae) has been used for treating inflammatory conditions such as muscle and joint pain, stiffness of tendon, and arthritis.43<\/sup><\/p>\n

Chandana – Santalum album<\/em><\/strong><\/p>\n

\u00a0<\/strong>Sandal is an age old medicinal plant and it is used for many diseases. It has curative roles such as anti hyperglycemic and anti hyper lipidemic, cardio protective, as a brain tonic and anti ulcerogenic.44,45<\/sup><\/p>\n

Rakta Chandana (Pterocarpus santalinus)<\/em><\/strong><\/p>\n

\u00a0<\/em>This plant is one of the oldest medicinals having properties like hepato protective, gastro protective, anticancer, antioxidant, anti diabetic and apoptotic.46<\/sup><\/p>\n

Vacha<\/strong> – Acorus calamus<\/em><\/strong><\/p>\n

\u00a0<\/em>The medicinal properties of A. calamus <\/em>were reported by Kumar and Vandana, 2012.47<\/sup> \u00a0<\/sup>This plant has activities like antiulcer and cyto – protective, analgesic, antispasmodic, anti-inflammatory, anticonvulsant and antibacterial.<\/p>\n

Chitraka – Plumbago zeylanica)<\/em><\/strong><\/p>\n

\u00a0<\/em><\/strong>This plant has medicinal roles such as antimicrobial, anti ulcer, anti obesity, anti-inflammatory, hypo cholestrolemic, hepato protective, wound healing, cytotoxic, anticancer and antiproliferative.48<\/sup><\/p>\n

Dhataki – Woodfordia fruticosa<\/em><\/strong><\/p>\n

\u00a0<\/em><\/strong>It was reported by Dubey et al<\/em>, 2014 that the presence of therapeutically potent antimicrobial compounds against MDR bacteria in Woodfordia fruticosa <\/em>and the crude leaf extract had no host toxicity on human lymphocytes.49<\/sup> n-butanol fraction of the extract was the most suitable bio-active fraction. The terpenes isolated were, phenol, 5-methyl-2-(1-methylethyl)-, phenol, 2-methoxy-4-(2-propenyl)-, 2, 6-octadien-1-ol, 3, 7-dimethyl-(E)-, 2, 6-octadienal, 3, 7-dimethyl-, cyclohexanol, and 2-methylene-5-(1-methylethenyl). The leaves have sedative properties and the juice of its fresh flowers, when applied on the head, supposed to reduce headache. The curative properties of Woodfordia <\/em>are due to the presence of secondary metabolites like alkaloids, flavonoids, glycosides, phenols, saponins, sterols etc. Grover and Patni, 2013 have identified 21 compounds in the GC MS analysis of Woodfordia <\/em>leaf extracts with important medicinal properties.50 <\/sup><\/p>\n

Sunthi<\/strong> – Zingiber officinalis<\/strong>)<\/em><\/p>\n

\u00a0<\/em>Ginger is also one of the household medicines used against common cold, cough and indigestion. Its medicinal values are well documented. Adel and Prakash, 2014, have reported its antioxidant properties. Ginger controls vomiting and nausea during pregnancy. It controls blood pressure by blocking calcium channels.51 <\/sup><\/p>\n

Magadhi – Piper longum<\/em><\/strong><\/p>\n

\u00a0<\/em><\/strong>Kumar et al.<\/em>, 2011 have reviewed the various health benefits of Piper longum<\/em>. with many important medicinal values such as anticancer, antioxidant, hepato protective, anti-inflammatory, immunomodulatory, antimicrobial, antihyperlipidemic, analgesic, antidepressant, anti amoebic, vasodialtory, bioavailability enhancer due the presence of<\/p>\n

piperine in it, anti obesity activity, radio protective, cardioprotective and antifungal activities.52<\/sup><\/p>\n

Pepper <\/strong>– Piper nigrum<\/em><\/strong><\/p>\n

Pepper plays a great role in digestions, useful for low appetite, sluggish digestion, abdominal pain, toxins and borborygmus.53<\/sup> Its anthelmentic qualities help remove worms. The drug stimulates the thermal receptors and increases secretion of salvia and gastric mucous. It has antimicrobial effect. It influences liver and metabolic function, and has insecticidal effect. It has other pharmacological activities like antioxidant, anticonvulsant, sedative, muscle relaxant, antipyretic, anti-inflammatory, antifungal, hepatoprotective, antimicrobial, antiulcer and lipolytic.54,55 <\/sup>Meghwal and Goswami, 2012\u00a0 have reviewed the chemical and physiological aspects of pepper.56<\/sup> \u00a0The dried or fried seeds are used for various culinary and medicinal use. In Ayurveda it is known as Kapha virodhini (works against Phlegm). The decoction of Pepper is used for treating cough.<\/p>\n

Twak \u2013 Cinnamon \u2013 Cinnamomum zeylanicum<\/em><\/strong><\/p>\n

Almost every part of the cinnamon tree has some medicinal or culinary use. Ranasinghe et al<\/em> 2013 and Jayaprakasha et al<\/em>, 2011, have reviewed the medicinal properties of Cinnamon.57,58<\/sup><\/p>\n

Ela – Cardamom – Elettaria cardamomum<\/em><\/strong><\/p>\n

Cardamom is another important culinary ingredient used for its characteristic aroma. Apart from the aroma it has medicinal value. Verma et al,<\/em> 2009, have reported blood pressure lowering, fibrinolysis enhancing and antioxidant activities of Cardamom.59 <\/sup>Khan et a<\/em>l, 2011 have shown the pharmacological basis of cardamom as medicine for asthma.60<\/sup><\/p>\n

Patra<\/strong> – Cinnamomum tamala<\/em> (Buch.-Ham.)<\/strong><\/p>\n

Its leaf and bark is used widely as flavouring agent in various culinary preparations. This tree is valued for its antioxidant, antimicrobial, antibacterial and antidiabetic activities.61 <\/sup><\/p>\n

Priyangu<\/strong> – Callicarpa macrophylla<\/strong><\/em>\u00a0<\/strong><\/p>\n

The Ayurvedic Pharmacopeia of India describes the fruits of\u00a0Callicarpa macrophylla<\/em>\u00a0Vahl<\/em>\u00a0<\/em>as an essential component of several ayurvedic formulations.62,63<\/sup> The plant has been reported to have various medicinal properties. The bark is used to heal cuts and wounds. Seeds and roots are used for digestion and leaves are used for rheumatism. The fruits are used for blisters and boils. The antimicrobial and anti-inflammatory activities of this plant have been already proved.64<\/sup> As many as 20 species from Callicarpa<\/em>\u00a0have reported ethnomedical uses, and several members among these are well known in the traditional medical systems of China and South Asia. Ethnomedical reports indicate their use in the treatment disorders like hepatitis, rheumatism, fever, headache, indigestion, and other ailments.65<\/sup> \u00a0The plant is already reported to have antibacterial, antidiabetic, analgesic and antipyretic, antifungal, anti-inflammatory and anti-arthritic activity.66<\/sup><\/p>\n

Nagakesara –Mesua ferrea <\/em>L<\/strong>.<\/em><\/p>\n

This medicinal role of this plant was reviewed by Chahar et al<\/em>, 2013.67<\/sup> It has medicinal activities like antioxidant and hepato protective, analgesic, antispasmodic, anti-venom, cancer chemotherapeutic, Immuno-modulatory, anti-neoplastic, anti-convulsant, anti-inflammatory, anti-ulcer and anti-microbial.68-79<\/sup><\/p>\n

Material And Methods<\/strong><\/p>\n

The medicine which is available in liquid form was subjected to GC MS analysis after necessary procedure. The metabolites in the samples were identified using a P2010 gas chromatography with thermal desorption system TD20 coupled with mass spectroscopy (Shimadzu). The ionization voltage 70ev and GC was conducted in the temperature programming mode with a Restek column (0.25mm, 60m, XTI-5). The temperature in the initial column was 80\u00b0C for 1 min, and then increased linearly to 70\u00b0C to 220\u00b0C held for 3 min followed by linear increased temperature 100\u00b0 C up to 290\u00b0C and held for 10min. The injection port temperature was 290\u00b0 C and the GC\/MS interface was maintained at 29\u00b0C, the samples were introduced via an all glass injector working in the split mode with helium carrier gas low rate with 1.2 ml per minute. The identification of metabolites was accomplished by comparison of retention time and fragmentation pattern with mass spectra in the NIST spectral library stored in the computer software (version 1.10 beta, Shimadzu) of the GC-MS. The relative percentage of each extract constituent was expressed with peak area normalization.<\/p>\n

Results And Discussion<\/strong><\/p>\n

The GC MS analysis graph is presented in Figure 1. Table 1 represents the retention time, percentage peak values, molecular formulae, molecular weights of possible types of compounds present in the GC MS analysis.<\/p>\n\n\n\n
\u00a0\"Figure<\/td>\nFigure 1: The GC MS profile of Aswagandharishtam.<\/strong><\/p>\n

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Click here to View figure<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

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 <\/p>\n\n\n\n
\"Table<\/td>\nTable 1: represents the retention time, percentage peak values, molecular formula, molecular weights of possible types of compounds present in the GC MS analysis.<\/strong><\/p>\n

 <\/p>\n

Click here to View\u00a0table<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

The possible medicinal roles of each of the compounds represented in the GC MS are mentioned below referring Dr.Duke\u2019s Phytochemical and Ethnobotanical Data base and others.<\/p>\n

1,1′-(1-Methyl-1,2-ethanediylidene) bis (cyanoacetohydrazide): Catechol-O-methyl-Transferae \u2013 Inhibitor and Methyl donar.<\/p>\n

Propanenitrile, 3-chloro: Not Known.<\/p>\n

Dichloroacetic acid, allyl ester: Acidifier, Arachidonic acid inhibitor, Increase aromatic amino. acid decarboxylase activity, inhibit uric acid production.<\/p>\n

Pyridine, 2,3,4,5-tetrahydro- Not Known.<\/p>\n

E-2-Octadecadecen-1-ol: Oligosaccharide provider, anticancer, antidote, Cytochrome-P450 2E1-Inhibitor, Decerease Epinephrine Production.<\/p>\n

Pyrrolidine, 1-(3-chloro-4,4,4-trifluoro-2-phenyl-2-butenyl)- \u00a0antiulcer, anti-tuberculosis and herbicidal activity.<\/p>\n

Tramadol: It is opioid pain medicine used for moderate to severe pain.<\/p>\n

Acetamide, N-(2-cyano-4,5-dimethoxyphenyl)-2-(pyrrolidin-1-yl)- Not known<\/p>\n

Cyclopropanecarboxylic acid, isobornyl ester: \u00a0Acidifier, Arachidonic acid inhibitor, Increase aromatic amino acid decarboxylase activity, inhibits uric acid production<\/p>\n

R-lavandulyl acetate: anti inflammatory<\/p>\n

Geranyl vinyl ether: anti-microbial, anticancer and antimalarial<\/p>\n

4-Amino-1, 5-pentandioic acid: Acidifier, Arachidonic acid inhibitor, Increase aromatic amino acid decarboxylase activity, inhibit uric acid production<\/p>\n

Ethyl hydrogen succinate: Hydrogen Peroxide Inhibitor, Succinate dehydrogenase\u00a0 inhibitor<\/p>\n

Dichloroacetic acid, allyl ester: Acidifier, Arachidonic acid inhibitor, Increase aromatic amino acid decarboxylase activity, inhibit uric acid production,<\/p>\n

1-(3-Acetamidophenyl)-3-(2,2,2-trichloro-1-isovaleramidoethyl)-2-thiourea : Not known<\/p>\n

3-Methyl-2-butenoic acid, propyl ester: Acidifier, Arachidonic acid inhibitor, Increase aromatic amino acid decarboxylase activity, inhibit uric acid production<\/p>\n

3-Phenylpropanol: Antibacterial<\/p>\n

Furan-2-one, 3,4-dihydroxy-5-[1-hydroxy-2-fluoroethyl]-17-Beta-hydroxysteroid \u2013dehydrgenase inhibitor,\u00a0 Testosterone Hydroxylase inhibitor.<\/p>\n

Pipradrol: Psychotic activity, anticonvulsant<\/p>\n

Formyl glutamine: Amino acid derivative<\/p>\n

Eugenol or Phenol, 2-methoxy-3-(2-propenyl): Synthetic Eugenol has been reported to have many important medicinal properties as is described by many reporters. It has medicinal roles such as antifungal, antioxidan, anticonvulsant and local anaesthetic, antistress and bacteriostatic, bactericidal, Anticarcinogenic, depresses activity of central nervous depressant, anti radiation, antiviral, induces apoptosis in melanoma cells and HL-60 leukemia cells.80-86<\/sup><\/p>\n

d-Mannose: \u00a017-beta- hydroxysteroid dehydrogenase inhibitor, Anticancer (Duodenum), Circulatory depressant, CNS- Depressant, \u00a0Coronary dilator, \u00a0Cyclin-D1-Inhibitor, Decongestant, Decrease endothelial platelet adhesion<\/p>\n

Desulphosinigrin: Not known<\/p>\n

L-Glucose: Glucode-6-Phosphate Inhibitor, Anti LDL, Decrease lactate, 12-Lipoxygenase-Inhibitor<\/p>\n

Asarone: is a known antifungal.87<\/sup><\/p>\n

Folic Acid: Acidifier, Arachidonic acid inhibitor, Increase aromatic amino acid decarboxylase activity, inhibit uric acid production.<\/p>\n

Pyrrolizin-1, 7-dione-6-carboxylic acid, methyl(ester): Acidifier, Arachidonic acid inhibitor, Increase aromatic amino acid decarboxylase activity, inhibit uric acid production.<\/p>\n

Gentamicin a: 5-Alpha Reductase inhibitor, Acetyle cholin anatagonist, AChE Inhibitor, Adaptogen.<\/p>\n

Tricyclo[4.4.0.0(2,7)]dec-8-ene-3-methanol, \u00e0,\u00e0,6,8-tetramethyl-, stereoisomer<\/p>\n

(-)-Spathulenol Antioxidant and anti-inflammatory activities.<\/p>\n

1H-Cycloprop[e]azulen-7-ol, decahydro-1,1,7-trimethyl-4-<\/p>\n

methylene-, [1ar-(1a\u00e0,4a\u00e0,7\u00e1,7a\u00e1,7b\u00e0)]- Antiobesity.<\/p>\n

Cyclopropanebutanoic acid, 2-[[2-[[2-[(2-<\/p>\n

pentylcyclopropyl)methyl]cyclopropyl]methyl]cyclopropyl]m: Antioxidant and anti diabetic.<\/p>\n

ethyl- methyl ester; Catechol-O-Methyl Transferase Inhibitor, Methyl Donor, Methyl Guanidine inhibitor.<\/p>\n

Methyl 9-methyltetradecanoate: Catechol-O-Methyl-Transferase Inhibitor, Methyl-Donor.<\/p>\n

Hexadecanoic acid, 14-methyl-, methyl ester: Catechol-O-Methyl-Transferase Inhibitor, Methyl-Donor.<\/p>\n

-Hexadecanoic acid: Hexadecanoic acid is reported to have activities like antioxidant, hypocholesterolemic, nematicide, antiandrogenic, as flavoring agents, hemolytic, antibacterial and cytotoxic and as 5-alpha reductase inhibitor.88,89<\/sup><\/p>\n

cis-Vaccenic acid: Acidifier, Arachidonic acid inhibitor, Increase aromatic amino acid decarboxylase activity, inhibit uric acid production.<\/p>\n

Oleic Acid: Antiinflamatory, Antiandrogenic, cancer preventive, hypercholesterolemic, 5-alpha reductase inhibitor.90<\/sup><\/p>\n

Trans-13-Octadecenoic acid: Acidifier, Arachidonic acid inhibitor, Increase aromatic amino acid decarboxylase activity, inhibit uric acid production<\/p>\n

Octadecanoic acid: Octadecanoic acid esters are reported to be antiviral,<\/p>\n

antibacterial and antioxidant activities.91<\/sup><\/p>\n

Squalene: Antibacterial, Antioxidant, Pesticide, Antitumor, Cancer preventive, Immunostimulant, Chemo preventive, Lipoxygenase-inhibitor.92<\/sup><\/p>\n

Piperine: Piperine has diverse biological and supportive therapeutic activities like radioprotective, immunomodulatory and anti tumor activities, antidepressant, anticonvulsant, antinocicetive, and anti-arthritic. It helps in the absorption of selenium, vitamin B and Beta carotene as well as other nutrients. Among the various properties of piperine, the most<\/p>\n

important is that it facilitates the bioavailability of medicines by depressing the activity of drug metabolizing enzymes.93<\/sup> Dendrite elongation inhibition activity was reported by Rao et al<\/em>, 2012.94<\/sup><\/p>\n

Cholesterol: Cholesterol is precursor for steroid synthesis and is a very important biomolecule.<\/p>\n

17-(1,5-Dimethylhexyl)-10,13-dimethyl-2,3,4,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-1Hcyclopenta[a]phenanthren-3-ol : Not known.<\/p>\n

Prostaglandin A2: Prostaglandin-Synthetase- Inhibitor, Prostaglandin Secretor,\u00a0 Inhibit AA-series- Prostaglandin\u00a0 synthesis<\/p>\n

7,9-Di-tert-butyl-1-oxaspiro(4,5)deca-6,9-diene-2,8-dione: Decalcifier, Decaboxylase inhibitor, DOPA decarboxylylase inhibitor, Histidine decarboxylase inhibitor, Coronary dialator.<\/p>\n

Pregn-4-en-3-one, 20-hydroxy-, (20R) – Antibiotic.<\/p>\n

Conclusions<\/strong><\/p>\n

The GC MS analysis of Aswagandharishtam has shown some promising molecules like prostaglandin A2, Cholesterol, Piperine, Gentamicin a, d-Mannose, Eugenol, Pipradrol etc. which have a activities similar to that of Aswagadharistham. This is a preliminary report and further work is on to prove the efficacy of this medicine by other parameters.<\/p>\n

Acknowledgements<\/strong><\/p>\n

The authors are indebted to all who have directly or indirectly helped in this work.<\/p>\n

Conflict of Interest<\/strong><\/p>\n

This is no conflict of interest.<\/p>\n

Funding Source<\/strong><\/p>\n

There is no funfing source<\/p>\n

 <\/p>\n

References<\/strong><\/p>\n

    \n
  1. Uddin Q, Samiulla L, Singh VK, Jamil SS. Phytochemical and pharmacological profile of Withania somnifera <\/em>Dunal: A review. J of Applied Pharmaceutical Sciences.<\/em> 2012;2(1):170-175.<\/li>\n
  2. Tohda C, Kuboyama T, Komatsu K. Search for natural products related to regeneration of the neuronal network. <\/em>Neurosignals<\/em>. 2005;14:34\u201345.
    \n    CrossRef<\/a><\/li>\n
  3. Ghosal S, Lal J, Srivastava R, Bhattacharya SK, Upadhyay SN, Jaiswal AK, Chattopadhyay U. Immunomodulatory and CNS effects of sitoindosides IX and X, two new glycowithanolides from\u00a0Withania somnifera<\/em>. Phytother Res<\/em>, 1989;3:201\u2013206.
    \n    CrossRef<\/a><\/li>\n
  4. Patil VN, Deokule S.S. Pharmacognostic study of\u00a0Chlorophytum tuberosum<\/em>\u00a0Baker. Int J Ayurveda Res<\/em>. 2010;1(4):237\u2013242.
    \n    CrossRef<\/a><\/li>\n
  5. Govindarajan R, Vijayakumar M, Pushpangadan P. Antioxidant approach to disease management and the role of \u2032Rasayana\u2032herbs of Ayurveda.\u00a0J Ethnopharmacol.<\/em> 2005; 99(2):165\u201378.
    \n    CrossRef<\/a><\/li>\n
  6. Dhuley J.N. Effect of some Indian herbs on macrophase functions in Ochratoxin A treated mice.\u00a0J Ethnopharmacol<\/em>. 1997;58:5\u201320.
    \n    CrossRef<\/a><\/li>\n
  7. Meena V, Chaudhary AK. Manjistha (Rubia cordifolia<\/em>) – A helping herb in cure of acne. Jour. of Ayurveda & Holistic Medicine.<\/em> 2015;III(II):11-17<\/li>\n
  8. Gorle A.M, Patil S.S. Evaluation of antioxidant and antiacne property of Rubia cordifolia.<\/em> Der Pharmacia Sinica<\/em>. 2013;1(3):59-63.<\/li>\n
  9. Khan N, Karodi R, Siddiqui A, Thube S, Rub R. Development of anti-acne gel formulation of anthraquinones rich fraction from Rubia cordifolia<\/em> (Rubiaceae). Int J Applied Res Natl Products.<\/em> 2012;4:28-36<\/li>\n
  10. Antarkar SS, Chinwalla T, Bhatt N. Anti-inflammatory activity of Rubia cordifolia<\/em> Linn. in rats. Indian J Pharmacol.<\/em> 1983;15:185-188.<\/li>\n
  11. Mariselvam R, Ranjitsingh A.J.A, Nanthini A.U.R. Preparation and characterization of silver nanoparticles using Rubia cordifolia<\/em> plant root extract and their microbial properties. Int J Adv Res.<\/em> 2013;1:56-61.<\/li>\n
  12. Lodia S, Kansala L. Antioxidant activity of Rubia cordifolia<\/em> against Lead toxicity. Int J Pharm Sci Res. 2012;3:2224-2232.<\/li>\n
  13. Bag A, Bhattacharya S.K, Chattopadhyay R.R. The development of Terminalia chebula <\/em>Retz. (Combretaceae) in clinical research. Asian Paci J Tropical Biomed.<\/em> 2013;3(-3): 244.<\/li>\n
  14. Sikha A, Harini A, Hegde P.L. Pharmacological activities of wild turmeric (Curcuma aromatica <\/em>Salisb): a review. Journal of Pharmacognosy and Phytochemistry<\/em>. 2015; 3(5):1-4.<\/li>\n
  15. Liu B, Gao Y.Q, Wang X.M, Wang Y.C, Fu L.Q. Germacrone inhibits the proliferation of glioma cells by promoting apoptosis and inducing cell cycle arrest. Mol Med Rep.<\/em> 2014; 10(2):1046-1050.
    \n    CrossRef<\/a><\/li>\n
  16. Sharma K, Bairwa R, Chauhan N, Srivastava B, Saini NK. Berberis aristata: A Review. Int J of Res in Ayurveda and Pharmacy.<\/em>2011;2(2):383-388.<\/li>\n
  17. Damle M. Glycyrrhiza glabra <\/em>(Liquorice)-a potent medicinal herb. International Journal of Herbal Medicine.<\/em> 2014;2(2);132-136.<\/li>\n
  18. Bhagwat D.P, Kharya M.D, Bani S, Kaul A, Kaur K, Chauhan P.S, Suri K.A, Satti N.K. Immunosuppressive properties of Pluchea lanceolata leaves. Ind J of Pharmacology.<\/em> 2010;42(1):21-26.
    \n    CrossRef<\/a><\/li>\n
  19. Nidhi Pandey,\u00a0Durgavati Yadav,\u00a0Vivek Pandey,\u00a0and\u00a0Yamini B. Tripathi. Anti-inflammatory effect of\u00a0Pueraria tuberosa<\/em>\u00a0extracts through improvement in activity of red blood cell anti-oxidant enzymes. Ayu.<\/em> 2013;(3):297\u2013301.<\/li>\n
  20. Tripathi Y.B, Nagwani S, Mishra P, Jha A, Rai S.P. Protective effect of\u00a0Pueraria tuberosa<\/em>\u00a0DC. embedded biscuit on cisplatin-induced nephrotoxicity in mice.\u00a0J Nat Med.<\/em>\u00a02012;66:109\u2013118.
    \n    CrossRef<\/a><\/li>\n
  21. Pandey N, Chaurasia J.K, Tiwari O.P, Tripathi Y.B. Antioxidant properties of different fractions of tubers from\u00a0Pueraria tuberosa<\/em>\u00a0Linn.\u00a0Food Chem.<\/em> 2007;105:19\u201322.
    \n    CrossRef<\/a><\/li>\n
  22. Hsu F.L, Liu I.M, Kuo D.H, Chen W.C, Su H.C, Cheng J.T. Antihyperglycemic effect of puerarin in streptozotocin-induced diabetic rats.\u00a0J Nat Prod.<\/em> 2003;66:788\u2013792.
    \n    CrossRef<\/a><\/li>\n
  23. Tanwar Y.S, Goyal S, Ramawat K.G. Hypolipidemic effects of tubers of Indian Kudzu (Pueraria tuberosa<\/em>).\u00a0J Herb Med Toxicol.<\/em> 2008;2:21\u201325.<\/li>\n
  24. Gupta RS, Sharma R, Sharma A. Antifertility effects of\u00a0Pueraria tuberosa<\/em>\u00a0root extract in male rats. Pharm Biol<\/em>. 2004;42:3\u20139.<\/li>\n
  25. Handa S.S, Kaul M.K. Recent development of some natural products. In: Handa SS, Kaul MK, editors.Supplement to Cultivation and Utilization of Medical Plants.\u00a0Jammu-Tawi: CSIR, RPL; 1996:53\u201396.<\/li>\n
  26. Khan R.A, Agarwal P.K, Kapil R.S. Puetuberosanol an epoxychalcanol from\u00a0Pueraria tuberosa<\/em>.Phytochemistry<\/em>. 1996;42:42\u201344.
    \n    CrossRef<\/a><\/li>\n
  27. Ram A, Lauria P, Gupta R, Kumar P, Sharma V.N. Hypocholesterolaemic effects of Terminalia arjuna <\/em>tree bark. J Ethnopharmacol<\/em>. 1997;55(3): 165-169.
    \n    CrossRef<\/a><\/li>\n
  28. Gupta R, Singhal S, Goyle A, Sharma V.N. Antioxidant and hypocholesterolaemic effects of Terminalia arjuna <\/em>tree bark powder: a randomised placebo-controlled trial. J Assoc Physicians India.<\/em> 2001;49:231-235.<\/li>\n
  29. Mandal A, Das K, Nandi DK. In vitro bioactivity study of bark extract of Terminalia arjuna <\/em>on probiotics, commercially available probiotic formulation. Int J Phytopharmacol.<\/em> 2010; 1(2):109\u2013113.<\/li>\n
  30. Mandal S, Patra A, Samanta A, Roy S, Mandal A, Das Mahapatra T, Pradhan S, Das K, Nandi DK. Analysis of phytochemical profile of Terminalia arjuna <\/em>bark extract with antioxidative and antimicrobial properties. Asian Pac J Trop Biomed.<\/em> 2013;3(12):960\u2013966.
    \n    CrossRef<\/a><\/li>\n
  31. Das K, Chakraborty P.P, Ghosh D, Nandi D.K. Protective effect of aqueous extract of Terminalia arjuna <\/em>against dehydrating induced oxidative stress and uremia in male rat. Iran J Pharma Res<\/em>. 2010;9(2):153\u2013161.<\/li>\n
  32. Nema R, Jain P, Khare S, Pradhan A, Gupta A, Singh D. Antibacterial and antifungal activity of Terminalia arjuna <\/em>leaves extract with special reference to flavonoids. Basic Res J Med Clin Sci.<\/em> 2012;1(5):63\u201365.<\/li>\n
  33. Biswas M, Biswas K, Karan TK, Bhattacharya S, Ghosh AK, Haldar PK. Evaluation of analgesic and anti-inflammatory activities of Terminalia arjuna <\/em>leaf. J Phytol.<\/em> 2011;3(1): 33\u201338.<\/li>\n
  34. Sivapalan S.R. Medicinal uses and Pharmacological activities of Cyperus rotundus<\/em> Linn \u2013 A Review. International Journal of Scientific and Research Publications<\/em>. 2013;3(5):1-8.<\/li>\n
  35. Duke J.A, Ayensu E.S. Medicinal Plants of China Reference Publications, Inc. 1985 ISBN 0-917256-20-4.<\/li>\n
  36. Chopra R.N, Nayar S.L, Chopra I.C. Glossary of Indian Medicinal Plants (Including the Supplement). Council of Scientific and Industrial Research. New Delhi. 1986.<\/li>\n
  37. Kohli K.R, Nipanikar SU, Kadbhane K.P. A comprehensive review on Trivrit (Operculina turpethum syn. Ipomoea turpethum<\/em>]. International Journal of Pharma and Bio Sciences<\/em>. 2010; 1(4):443-452.<\/li>\n
  38. Suresh Kumar S.V, Sujatha C, Shymala J, Nagasudha B, Mishra S.H. Protective effect of Root Extract of Operculina terpethum<\/em> Linn. Against Paracetamol induced Hepatotoxicity in Rats. Indian Journal of Pharmaceutical Sciences<\/em>. 2006;68 (1):32-35.
    \n    CrossRef<\/a><\/li>\n
  39. Md. Harun-or-Rashid, Gafur MA, Md. Golam Sadik, Md., Aziz Abdur Rahman. Antibacterial and Cytotoxic Activities of Extracts and Isolated Compounds of Ipomoea turpethum. Pakistan Journal of Biological Sciences<\/em>. 2002;5(5):597-599.
    \n    CrossRef<\/a><\/li>\n
  40. Anbuselvam C, Vijayavel K, Balsubramaniyan M.P. Protective effect of Operculina<\/em> turpethum<\/em> against 7, 12 dimethylbenz(a)anthracene induced oxidative stress with reference to breast cancer in experimental rats. Chemico- Biological Interactions<\/em>. 2007;168:229-236.
    \n    CrossRef<\/a><\/li>\n
  41. Krishnaraju A.V, Rao T.V.N, Sundararaju D, Vanisree M, Hsin Sheng Tsay, \u00a0\u00a0Subbaraju GV. Assessment of Bioactivity of Indian Medicinal Plants Using Brine Shrimp (Artemia salina<\/em>) Lethality Assay. International Journal of Applied Science and Engineering. <\/em>2005;3(2):125-34.<\/li>\n
  42. Chatterjee S, Banerjee A, Chandra I. Hemidesmus indicus<\/em>: A Rich Source of Herbal Medicine. Med Aromat Plants.<\/em> 2014:3-4.<\/li>\n
  43. Hanprasertpong N, Teekachunhatean S,\u00a0Chaiwongsa R,\u00a0Ongchai S,\u00a0Kunanusorn P,\u00a0Sangdee C,\u00a0Panthong A,\u00a0 Bunteang S,\u00a0Nathasaen N, Reutrakul V. <\/sup>Analgesic, Anti-Inflammatory, and Chondroprotective Activities of\u00a0Cryptolepis buchanani<\/em>\u00a0Extract:\u00a0In Vitro\u00a0and\u00a0In Vivo\u00a0Studies. BioMed Research International Volume\u00a02014\u00a0(2014), Article ID\u00a0978582, 8 pages.http:\/\/dx.doi.org\/10.1155\/2014\/978582
    \n    CrossRef<\/a><\/li>\n
  44. Khan M.S, Singh M, Khan M.A, Sayeed Ahmad. Protective effect of Santalum album <\/em>on doxorubicin induced cardiotoxicity in rats. World Journal of Pharmaceutical Research.<\/em> 2014;3(2):2760-2771.<\/li>\n
  45. Ahmad N, Khan M.S.A, Jais A.M.M, Mohtaruddin N, Ranjbar M, Amjad S.M, Nagaraju B, Pathan M. Anti-ulcer Activity of\u00a0 Sandalwood (Santalum album<\/em> L.) Stem Hydroalcoholic Extract in Three Gastric-Ulceration Models of Wistar Rats. Bol Latinoam Caribe Plant Med Aromat.<\/em> 2013;12(1):81-91.<\/li>\n
  46. Azamthulla M, Balasubramanian R, Kavimani S.\u00a0 A review on Pterocarpus santalinus <\/em>linn. World<\/em> Journal of Pharmaceutical Research<\/em>. 2015;4 (2):282-292.<\/li>\n
  47. Kumar A, Vandana. Medicinal properties of Acorus calamus<\/em>. Journal of Drug Delivery & Therapeutics.<\/em> 2013;3(3):143-144.
    \n    CrossRef<\/a><\/li>\n
  48. Sharma A, Singh N. International Journal of Recent Scientific Research<\/em>. 2015;6(6):4825-4829.<\/li>\n
  49. Dubey D, Patnaik R, Ghosh G, Padhy RN. In\u00a0Vitro<\/em>\u00a0Antibacterial Activity, Gas Chromatography\u2013Mass Spectrometry Analysis of\u00a0Woodfordia fruticosa<\/em>\u00a0Kurz. Leaf Extract and Host Toxicity Testing With\u00a0In\u00a0Vitro<\/em>\u00a0Cultured Lymphocytes From Human Umbilical Cord Blood Osong Public Health and Research Perspectives.\u00a02014;5(5):298.<\/li>\n
  50. Grover N, Patni V. Phytochemical characterization using various solvent extracts and GC-MS analysis of methanolic extract of Woodfordia fruticosa<\/em> (L.) Kurz. leaves. Int J Pharm Pharm Sci.<\/em> 2013;5(4):291.<\/li>\n
  51. Adel PRS, Prakash J. Chemical composition and antioxidant properties of ginger root (Zingiber officinalis<\/em>). Journal of Medicinal Plants Research<\/em>. 2010;4(24):2674-2679.
    \n    CrossRef<\/a><\/li>\n
  52. Kumar S, Kamboj J, Suman, Sharma S. Overview for various aspects of the health benefits of Piper longum <\/em>Linn. fruit. J of Acupuncture and Meridian Studies.<\/em> 2011;4(2): 134-140.
    \n    CrossRef<\/a><\/li>\n
  53. Shamkuwar P.B, Shahi S.R, Jadhav S.T. Evaluation of anti diarrheal effect of Black pepper (Piper nigrum<\/em> L.). Asian Journal of Plant Science and Research. 2012;2 (1):48-53.<\/li>\n
  54. Gruenwald\u00a0 J. \u00a0<\/em>Medicines PDR for Herbal. 1st Ed. Physicians Desk Reference Inc., Montvale, New Jersey, 1998: 850-852.<\/li>\n
  55. Sharma P.C. et al<\/em>., Medicinal Plants Used in Ayurveda. Central Council of Ayurveda and Siddha, New Delhi, India. 2002.<\/li>\n
  56. Meghwal M, Goswami T.K. Chemical Composition, Nutritional, Medicinal and Functional Properties of Black Pepper: A Review. 1; 2012: 172. doi:10.4172\/scientificreports.17<\/li>\n
  57. Ranasinghe P, Pigera S,\u00a0Sirimal G.A, Galappaththy P.P,\u00a0Constantine G.R,\u00a0Prasad K.\u00a0 Medicinal properties of \u2018true\u2019 cinnamon (Cinnamomum zeylanicum<\/em>): a systematic review. BMC Complementary and Alternative Medicine.<\/em> 2013; 3<\/strong>:275.
    \n    CrossRef<\/a><\/li>\n
  58. Jayaprakasha G.K, Rao L.J. Chemistry, biogenesis, and biological activities of Cinnamomum zeylanicum<\/em>. Crit Rev Food Sci Nutr<\/em>.2011;51:547\u2013562.
    \n    CrossRef<\/a><\/li>\n
  59. Verma S.K, Jain V, Katewa S.S. Blood pressure lowering, fibrinolysis enhancing and antioxidant activities of cardamom (Elettaria cardamomum<\/em>). Indian Journal of Biochemistry and Biophysics.<\/em> 2009;46(6):503-506.<\/li>\n
  60. Khan A, Khan Q.J, Gilani A.\u00a0 Pharmacological basis for the medicinal use of cardamom in asthma. Bangladesh J Pharmacol<\/em> 2011;6:34-37.
    \n    CrossRef<\/a><\/li>\n
  61. Preety A, Sharma S. A Brief Review on Cinnamomum tamala<\/em> (Buch.-Ham.) Nees & Eberm.: An Important Medicinal Tree. International Journal of Research in Biological Sciences.<\/em> 2016;6(2): 26-31.<\/li>\n
  62. Jayaraman S, Variyar E.J. Evaluation of immunomodulatory and antioxidant activities of polysaccharides isolated from\u00a0<\/strong>Callicarpa macrophylla VAHL. <\/strong><\/em>Int J Pharm Pharm Sci.<\/em> 2015;7(9) 357-360.<\/li>\n
  63. Verma V.K, Siddiqui N.U, Mohammad Aslam. Isolation of 3\u03b2, 16\u03b1, 17-trihydroxyphyllocladane from leaves of\u00a0Callicarpa macrophylla Vahl<\/em>. Natr Prod Med Preliminary<\/em> 2011;(3 Suppl 5);1-3.<\/li>\n
  64. Yadav V, Jayalakshmi S, Singla R.K, Patra A, Khan S. Assessment of anti-inflammatory and analgesic activities of\u00a0Callicarpa macrophylla Vahl.<\/em>\u00a0roots extract. Webmed Central Pharmacol.<\/em> 2012;3 (Supp 5):1-7.<\/li>\n
  65. William P, Jonesand A, Kinghorn D. Biologically active natural products of the genus Callicarpa<\/em>. Curr Bioact Compd,2008; 4;(Suppl 1):15\u201332.
    \n    CrossRef<\/a><\/li>\n
  66. Soni R.K, Dixit V, Irchhaiya R, Alok S.\u00a0Callicarpa macrophylla:\u00a0<\/em>a review update on its botany, ethnobotany, Phytochemistry and Pharmacology. IJP<\/em>. 2014;1:87-94.<\/li>\n
  67. Chahar K, Kumar S.D.S, Geetha L, Lokesh T, Manohara KP. Mesua ferrea<\/em> L.: A review of the medical evidence for its phytochemistry and pharmacological actions. African Journal of Pharmacy and Pharmacology.<\/em> 2013;7(6):211-219.
    \n    CrossRef<\/a><\/li>\n
  68. Jayanthi G, Kamalraj S, Kannan Karthikeyan, Muthumary J. Antimicrobial and antioxidant activity of the endophytic fungus Phomopsis sp. GJJM07 isolated from Mesua ferrea<\/em>. Int J Curr Sci.<\/em> 2011; 1:85-90.<\/li>\n
  69. Garg S, Kameshwar S, Rajeev R, Pankaj A, Parshuram M. In vivo Antioxidant activity and hepatoprotective effects of methanolic extracts of Mesua ferrea<\/em> L. Int. J. Pharmatechnol Res<\/em>. 2009;1:1692-1696.<\/li>\n
  70. Hassan T.M, Ali M.S, Alimuzzaman M, Raihan S.Z. Analgesic activity of Mesua ferrea<\/em> Linn. Dhaka Univ J Pharm Sci.<\/em> 2006;5:73-75.<\/li>\n
  71. Prasad D.N, Basu S.P, Srivastava A.K. Antispasmodic activity of the crude and purified oil of Mesua ferrea<\/em> seed. Anc Sci Life.<\/em> 1999;19:74-75.<\/li>\n
  72. Uawonggul N, Chaveerach N, Thammasirirak N, Arkaravichien T, Chuachan C, Daduang S. Screening of plants acting against Heterometrus laoticus scorpion venom activity on fibroblast cell lysis. J Ethnopharmacol.<\/em> 2006;103:201-207.
    \n    CrossRef<\/a><\/li>\n
  73. Saxena A, Dixit S, Aggarwal S, Seenu V, Prashad R, Bhushan SM, Tranikanti V, Misra MC, Srivastava A. An ayurvedic Herbal compound to reduce toxicity to Cancer chemotherapy: A randomized controlled trail. Indian J Med Paediat Oncol.<\/em> 2008;29:11-18.
    \n    CrossRef<\/a><\/li>\n
  74. Chahar M.K, Sanjaya Kumar D.S, Lokesh T, Manohara KP. In vivo antioxidant and immunomodulatory activity of mesuol isolated from Mesua ferrea<\/em> L. seed oil. Int Immunopharmacol.<\/em> 2012;13:386-391.
    \n    CrossRef<\/a><\/li>\n
  75. Mahavorasirikul W, Viyanant V, Chaijaroenkul W, Itharat A, NaBangchang K. Cytotoxic activity of Thai medicinal plants against human cholangiocarcinoma, laryngeal and hepatocarcinoma cells in vitro. BMC Compl Altern Med, 2010; 10: 55.
    \n    CrossRef<\/a><\/li>\n
  76. Tiwari P.K, Irchhaiya R, Jain SK. Evaluation of anticonvulsant activity of Mesua ferrea<\/em> Linn. ethanolic flower extract. Int J Pharm Life Sci.<\/em> 2012;23:1507-1509.<\/li>\n
  77. Gopalakrishnan C, Shankarnarayanan D, Nazimudeen S.K, Viswanathan S, Kameswaran L. Anti-inflamatory and CNS depressant activities of xanthones from Calophyllum inophyllum <\/em>and Mesua ferrea. Ind\u00a0 J Pharmacol.<\/em> 1980;12:181-191.<\/li>\n
  78. Jalalpure S.S, Yuvaraj D, Mandavkar, Pallavi R, Khalure, Gulab S, Shinde, Pournima A, Shelar, Amol S, Shah. Antiarthritic activity of various extracts of Mesua ferrea<\/em> Linn. seed. J. Ethnopharmacol.<\/em> 2011;138:700-704.
    \n    CrossRef<\/a><\/li>\n
  79. Mazumder R, Sujata G. Dastidar, Basu S.P, Avijit Mazumder, Singh SK. Antibacterial Potentiality of Mesua ferrea<\/em> Linn. flowers. Phytother Res.<\/em> 2004;18:824-826.
    \n    CrossRef<\/a><\/li>\n
  80. Lee SJ, Han J.I, Le G.S, Park M.J, Ghoi IG, Na KJ, Jeung E.B. Antifungal effect of engenol and nerolidol against Microsporum gypseum <\/em>in a guinea pig model. Biol Pharm Bull<\/em>. 2007;30:184-188.
    \n    CrossRef<\/a><\/li>\n
  81. Gulcin I. Antioxidant activity of eugenol: A structure \u2013activity relationship study. Journal of Medicinal Food.<\/em> 2011;14(9):975-985.
    \n    CrossRef<\/a><\/li>\n
  82. Dallmeier K, Carlini C.A. Anesthetic, Hypothermic, myo-relaxant and anticonvulsant effects of synthetic eugenol derivatives and natural analogues. Pharmacology.<\/em> 1981;22: 113-127.
    \n    CrossRef<\/a><\/li>\n
  83. Zheng G.Q, Kenney PM, Lam L.K. Sesquiterpenes from clove (Eugenia caryophyllata<\/em>) as potential anticarcinogenic agents. J Nat Prod.<\/em> 1992;55:999-1003.
    \n    CrossRef<\/a><\/li>\n
  84. Brodin P, Roed A. Effects of eugenol on rat phrenic nerve and phrenic nerve-diaphragm preparations. Arch Oral Biol.<\/em> 1984:611-615.
    \n    CrossRef<\/a><\/li>\n
  85. Pandey B.N, Lathika K.M, Mishra K.P. Modification of radiation induced oxidative damage in liposormal and microsomal membrane by eugenol. Rad Physics Chem<\/em>. 2006; 75:384-391.
    \n    CrossRef<\/a><\/li>\n
  86. Okada N, Hirata A, Murakami Y, Shoji M, Sakagami H, Fujisawa S. Anticancer Res<\/em>. 2005; <\/em>25:3263-3270.<\/li>\n
  87. Yun B, Hwang BK. Antifungal activity of \u03b2- Asarone from rhizomes of Acorus gramineus<\/em>. J Agric Food Chem<\/em>. 2004;52(4):776-78.
    \n    CrossRef<\/a><\/li>\n
  88. Kumar G, Kumar R. GC-MS evaluation of bioactive molecules from the methanolic leaf extract of Azadirachta indica <\/em>(A.JUSS). Asian J of Pharmaceutical Science & Tech.<\/em> 2015; 5(2):64.<\/li>\n
  89. Rajeswari G, Murugan M, Mohan VR. GC-MS analysis of bioactive components of Hugonia mystax <\/em>L. bark (Linaceae). J Pharm Biomed Sci<\/em>. 2013;29:818-824.<\/li>\n
  90. Gopalakrishna S. GC MS analysis of some bioactive constituents of Mussaenda frondasa <\/em>Linn. Int J of Pharma and Bio Sciences<\/em>. 2011;2(1):313-320.<\/li>\n
  91. Sudharsan S, Saravanan, Shanmugam A, Vairamani S, Mohan Kumar R, Menaga S, Ramesh N. Isolation and Characterization of Octadecanoic Acid from the Ethyl Acetate Root Extract of Trigonella foneum graecum <\/em>L. by Using Hydroponics Method. J Bioterr Biodef.<\/em> 2010;2:105. doi: 10.4172\/2157-2526.1000105.
    \n    CrossRef<\/a><\/li>\n
  92. Sermakkani M. Thangapandian V. GC-MS Analysis of Cassia italica <\/em>leaf methanol extract. Asian Journal of Pharmaceutical and Clinical Research<\/em>. 2012;5(2):90-94.<\/li>\n
  93. Demirayak S, Karaburun AC, Beis R. Some pyrrole substituted aryl pyridazinone and phthalazinone derivatives and their antihypertensive activities. Eur J Med Chem.<\/em> 2004; 39:1089\u20131095.
    \n<\/em>    CrossRef<\/a><\/li>\n
  94. Rao G.V, Rao K.S, Mukhopadhyay T, Madhavi MSL. Alkamides and their biological activity from Piper longum Linn. Journal of Pharmacy Research. <\/em>2012;5(1):165-168.<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

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