{"id":20985,"date":"2018-06-25T10:08:08","date_gmt":"2018-06-25T10:08:08","guid":{"rendered":"http:\/\/biomedpharmajournal.org\/?p=20985"},"modified":"2018-10-06T05:20:44","modified_gmt":"2018-10-06T05:20:44","slug":"herbendodontics-phytotherapy-in-endodontics-a-review","status":"publish","type":"post","link":"https:\/\/biomedpharmajournal.org\/staging\/vol11no2\/herbendodontics-phytotherapy-in-endodontics-a-review\/","title":{"rendered":"Herbendodontics\u2013Phytotherapy In Endodontics: A Review"},"content":{"rendered":"

Introduction<\/strong><\/p>\n

The potential role of microorganisms in the initiation and progression of endodontic infections has long been established. So, the main objective of endodontic treatment is either prevention or repair of periapical pathosis caused by these microorganisms.1,2 <\/sup>In order to\u00a0 achieve complete elimination of infection and to promote helaing,\u201cchemo-mechanical preparation\u201d of the tooth is carried out by mechanical instrumentation of the root canal space with simultaneous use of chemical agents.3<\/sup><\/p>\n

Nowadays there has been an increasing trend to seek herbal alternatives for endodontic treatment. This way of use of herbals for curing various diseases is known as \u201cPhytotherapy or Phytomedicine or Ethnopharmacology\u201d. In recent years, there has been an extreme growth in the field of herbal medicine due to their beneficial properties, ease of availability and less side effects.4<\/sup><\/p>\n

The root canal anatomy is complex and the challenging part of endodontic treatment is to disinfect this complex system most effectively. For this purpose, irrigants and intracanal medicaments are used in endodontics which primarily reduces the microorganisms inside the root canal system. The most commonly used irrigants in endodontics are sodium hypochlorite (NaOCl), ethylene-diamine-tetra-acetic acid (EDTA) solution and chlorhexidine (CHX) which can cause harmful side effects. Intracanal medicaments such as calcium hydroxide causes collagen breakdown and leads to weakening of radicular dentin. Triple antibiotic paste containing minocycline, metronidazole and ciprofloxacin have potential side effects of tooth discoloration and also cause demineralisation of dentin.5-7<\/sup><\/p>\n

In the search for novel irrigants and intracanal medicaments with good biocompatibility and antimicrobial activity, researchers have explored a number of potential agents of natural origin. India has rich source of medicinal plants that are widely distributed throughout the country.1<\/sup>\u00a0In ancient times, they believed that right herb in the right combination keeps the body system in harmony. Herbs contains different components such as essential oils, flavonoids, tanins and alcohols through which it attains varying degree of medicinal values. 4<\/sup><\/p>\n

Plants, animals & minerals are all sources of phytotherapeutic agents. Many herbs have potential use in endodontics which can be used with minimal incidences of complications.<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
S.No<\/strong><\/td>\nPhytomedicines Used In Endodontics<\/strong><\/td>\nActive Ingredients<\/strong><\/td>\n<\/tr>\n
1.<\/td>\nAzadiarachta indica<\/em> \u2013 Neem<\/td>\nNimbin, nimbidinin, nimbolide and nimbidinic acid<\/td>\n<\/tr>\n
2.<\/td>\nMelaleuca alternifolia<\/em> \u2013 Tea tree oil<\/td>\nTerpinen-4-ol<\/td>\n<\/tr>\n
3.<\/td>\nCurcuma longa<\/em> \u2013 Turmeric<\/td>\nCurcuminoids, tumerone, atlantone & zingiberone<\/p>\n

 <\/td>\n<\/tr>\n

4.<\/td>\nMorinda citrifolia<\/em> \u2013 Noni fruit:<\/p>\n

 <\/td>\n

Acubin, L-asperuloside, atizarin & anthraquinone<\/p>\n

 <\/td>\n<\/tr>\n

5.<\/td>\nTriphala<\/td>\nTannins, quinones, flavonoids, gallic acid and vitamin C.<\/td>\n<\/tr>\n
6.<\/td>\nSyzigium aromaticum – <\/em>\u00a0Clove<\/td>\nEugenol, isoeugenol and vanillin.<\/td>\n<\/tr>\n
7.<\/td>\nAcacia nilotica –\u00a0 Babool<\/p>\n

 <\/td>\n

Tannins, phenolic compounds, essential oils & flavonoids<\/td>\n<\/tr>\n
8.<\/td>\nAllium sativa – <\/em>\u00a0Garlic<\/td>\nAllicin<\/td>\n<\/tr>\n
9.<\/td>\nZingiber officinale \u2013 Ginger<\/td>\nZingerone, gingerol & 6-shagoal<\/td>\n<\/tr>\n
10.<\/td>\nPropolis \u2013 Honey bee wax<\/td>\nFlavonoids and cinnamic acid derivatives<\/td>\n<\/tr>\n
11.<\/td>\nAloe barbadenis<\/em> \u2013 Aloe vera<\/td>\nAloin and aloe-emodin<\/td>\n<\/tr>\n
12.<\/p>\n

 <\/p>\n

 <\/td>\n

Agaricus bisporus – <\/em>\u00a0Mushroom<\/td>\nPlectasin, confuentin, grifolin and neogrifolin<\/td>\n<\/tr>\n
13.<\/td>\nArctium lappa<\/em><\/td>\nSesquiterpene lactones and\u00a0 inulin<\/td>\n<\/tr>\n
14.<\/td>\nGlycyrrhiza glabra – <\/em>\u00a0Liquorice<\/td>\nTriterpenoid compound – Glycyrrhizin<\/td>\n<\/tr>\n
15.<\/td>\nRhus plants<\/td>\nTannins & gallic acids<\/td>\n<\/tr>\n
16.<\/td>\nVaccinium macrocarpon – <\/em>\u00a0Cranberry<\/td>\nFlavonoids, phenolic acids, anthocyanins and condensed tannins<\/td>\n<\/tr>\n
17.<\/td>\nPsidium guajava<\/em> –\u00a0 Guava<\/td>\nGuajaverin<\/td>\n<\/tr>\n
18.<\/td>\nRiccinus communis <\/em>– Castor<\/td>\nRicinoleic acid<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Herbal Alternatives Used As Irrigants In Endodontics<\/strong><\/p>\n

Azadiarachta indica<\/em><\/strong> \u2013 Neem<\/strong><\/p>\n

It is also called as \u201cIndian neem \/ Margosa tree \/ Indian lilac\u201d. This medicinal tree common in India, is considered holy. US National Academy of Sciences entitled neem as \u201ca tree for solving global problems\u201d as it produces numerous biological activities.8<\/sup><\/p>\n

Tetranortriterpenes extracted from neem are nimbin, nimbidinin, nimbolide and nimbidinic acid. The crude extracts from their leaves, bark, flowers, roots and seeds have various pharmacologic actions. It possess a significant antibacterial action against many gram positive and gram negative microorganisms such as Streptococcus mutans<\/em>, M.tuberculosis, Streptomycin resistant strains, M.pyogenes, Vibrio cholera <\/em>& Klebsiella pneumonia.<\/em>9<\/sup><\/p>\n

From the studies done by Bohra et al, it\u00a0 reveals us that the aqueous and ethanolic extract of neem leaf has potential action against E. faecalis<\/em>. It is as effective as NaOCl.10<\/sup>\u00a0So, neem can be used as a potential alternative to sodium hypochlorite against endodontic microflora.<\/p>\n

Melaleuca alternifolia<\/em><\/strong> \u2013 Tea tree oil<\/strong><\/p>\n

This tree is native to wet lowland locations of Australia. Its height is usually 10-25 feet and has a papery white bark, dark green needle-like leaves and colourful blossoms. It possess great antibacterial and antifungal properties due to the presence of its major active component terpinen-4-ol (30%-40%). It has also mild tissue solvent action which can be used for dissolving necrotic pulp tissue.11<\/sup><\/p>\n

In an in vitro study done by Sadr Lahijani, it is demonstrated that tea tree oil is as effective as NaOCl and less toxic than NaOCl.12<\/sup><\/p>\n

Curcuma longa<\/em><\/strong> \u2013 Turmeric<\/strong><\/p>\n

Turmeric belongs to Zingiberaceae family which is a perennial plant with small stem, large oblong leaves and bears ovate brownish yellow rhizomes. This popular Indian herb is used since ancient times as food preservatives, colouring material and in traditional medicine for treating sprains and swelling caused by injury. Turmeric contains polyphenols like curcuminoids [curcumin (diferuloylmethane), dimethoxycurcumin & bisdimethoxycurcumin] and various volatile oils like tumerone, atlantone & zingiberone.13\u00a0<\/sup>In this curcumin is the main yellow pigment which is responsible for antimicrobial, anti-inflammatory, antioxidant, anticarcinogenic, anti mutagenic, antifungal and anti \u2013coagulant activities.<\/p>\n

The antimicrobial action of turmeric is due to the reason that curcumin destroys the assembly of a protein-filamenting temperature-sensitive mutant Z (FTSZ) profilaments and enhances the GTPase activity of FTSZ which are all deterimental to bacteria.14<\/sup>\u00a0As an irriganting solution, turmeric prevents the formation of biofilms. This is because it eliminates the extracellular polymeric substance matrix which serves as the source of nutrient or substrate for further cell growth of bacteria.15<\/sup><\/p>\n

Many in vitro studies have proved the beneficial effects of curcumin when used as aqueous solutions against gram positive bacterias like E. faecalis<\/em>, Streptococcus intermedius<\/em> and other gram negative bacteria like E. coli.<\/em> Prasanna Neelakantan et el done an in vitro study to compare the antimicrobial activity of curcumin and NaOCl against E. faecalis <\/em>which showed that curcumin has similar antimicrobial action as NaOCl.16<\/sup>\u00a0In 2013, Hemanshi Kumar et al designed an in vitro study in which they found that curcumin extract showed larger zones of microbial inhibition against the E.faecalis <\/em>anaerobes. Hence, it can be used as an endodontic irrigant especially in retreatment cases.17<\/p>\n

Morinda citrifolia<\/em><\/strong> \u2013 Noni fruit<\/strong><\/p>\n

Noni also known as Indian Mulberry, nono or nonu, BaJiTian, cheese fruit and Nhau in various countries across the world. They are indigenous in tropical countries. It is discovered by Polynesians and it has been used as one of the traditional folk medicinal plants over 2000 years.18<\/sup><\/p>\n

A variety of compounds have been identified from Noni fruit such as Acubin, L-asperuloside, atizarin and also some anthraquinone compounds which are responsible for its \u00a0antimicrobial activity against infectious bacterial strains like Pseudomonas aeruginosa, Proteus morgaii and Shigella<\/em>.19\u00a0<\/sup>Besides this, it also exhibits anti tumor, anti-inflammatory, analgesic, anthelmintic and immune-enhancing properties.20<\/sup><\/p>\n

In 2008, Murray et al conducted a study to evaluate the efficacy of Morinda citrifolia juice (MCJ) as an irrigating solution. The results of the study showed that MCJ was same as NaOCl in conjuction with EDTA, in removing smear layer from the instrumented root canal walls.21\u00a0<\/sup>It was the first juice to be indicated as a possible alternative to NaOCl as an endodontic irrigant as it is biocompatible & antioxidant in nature.<\/p>\n

Triphala\u00a0<\/strong><\/p>\n

It is an ayurvedic combination of dried and powdered fruits of three herbal plants namely Terminalia bellerica<\/em> (Bibhitaki), Terminalia chebula<\/em> (Halituki) and Emblica officinalis<\/em> (Amalaki). Triphala consists of tannins, quinones, flavonoids, gallic acid and vitamin C. Bacterioststic and bactericidal properties of triphala are mainly because of tannic acid. It can be used as chelating agent due to the presence of citric acid in its fruits. Dimethyl sulfoxide was used as a solvent for triphala.<\/p>\n

Emblica officinalis<\/em> has got antimicrobial and cytotoxic effects whereas ethanolic extract of Terminalia chebula<\/em> has broad spectrum activity against different bacterias like Salmonella typhi, Staphylococcus epidermidis & Bacccilus<\/em> subtilis<\/em>. Terminalia bellerica<\/em> was found to be highly potential when compared to other two fruits. It also has more potency against E. faecalis.<\/em>22<\/sup>\u00a0In a study conducted by Prabhakar et al in 2010, revealed that triphala was effective against root canal biofilms and are similar to NaOCl & doxycycline based irrigating agents. This is because its free radical scavenging action makes it an efficient tool to prevent formation of biofilm.23<\/sup><\/p>\n

Syzigium aromaticum<\/em><\/strong> \u2013 Clove\u00a0<\/strong><\/p>\n

Essential oils of clove are eugenol, isoeugenol and vanillin which exhibits antioxidant, antibacterial and anodyne effects. Clove oil has sedative action on pulpal inflammation.24<\/sup><\/p>\n

Gupta et al made an SEM study to evaluate the effectiveness of three different plant extracts namely Syzigium aromaticum <\/em>(clove), Ocimum sanctum<\/em> (tulsi) and Cinnamum zeylanicum<\/em> (cinnamon) when used as an endodontic irrigant. The study results revealed that among the experimental groups tested, Syzigium aromaticum along with EDTA was the most effective in removing smear layer.25<\/sup><\/p>\n

Acacia nilotica<\/em><\/strong> \u2013 Babool\u00a0<\/strong><\/p>\n

Babool also called as Gum Arabic tree is common species found in Indian and African sub-continent. It consists of tannins, phenolic compounds, essential oils & flavonoids which are all responsible for its antimicrobial antioxidant, antifungal, antiviral and antibiotic functions.<\/p>\n

Rosina Khan et al revealed that this tree possess effective antimicrobial action against Streptococcus mutans<\/em> and E. faecalis<\/em>.26<\/sup>\u00a0Dhanya Kumar et al in 2011 investigated the antibacterial activity of four plant extracts namely clove, cinnamon, liquorice and babool and stated that babool is most effective in erradicating E. faecalis organisms when used at 50% concentration.27<\/sup><\/p>\n

Allium sativa \u2013 <\/em><\/strong>Garlic\u00a0<\/strong><\/p>\n

The bulbs of garlic or Amaryllidaceae has been used medicinally as antimicrobial & antihyperlipidemic agent since ancient times. It has also numerous other effects such as detoxification, antioxidant, anticarcinogenic, anticoagulant, antihypertensive, antibacterial, antifungal, antiviral and anti-inflammatory.28<\/sup><\/p>\n

The active ingredient of garlic is allicin which makes garlic a popular antimicrobial agent and produces immunological functions. Allicin has detrimental action on the cell wall and cell membrane of the bacterias. It was found that the ethanolic extract of garlic are effective against Stahylococcus aureus<\/em> and it exhibits both bacteriostatic & bactericidal activity.29<\/sup>\u00a0Also it was shown that the aqueous solution of garlic has antibacterial effect against 17-multidrug-resistant bacterial isolates. So this can be utilised as an herbal alternative to NaOCl. 30<\/sup><\/p>\n

In 2014, a study was done by Basma A. Alrazhi et al to evaluate the antibacterial activity of garlic and ginger as irrigant. In that garlic extract reported to inhibit the formation of biofilms by Streptococcus epidermidis<\/em> and exhibited antibacterial efficacy against E. faecalis. <\/em>31<\/sup><\/p>\n

Zingiber officinale<\/em><\/strong> \u2013 Ginger<\/strong><\/p>\n

The rhizomes of Zingiberaceae are commonly used ingredient in large number of foods & beverages across the world. They was found to have anti-inflammatory, analgesic, antipyretic, antimicrobial and hypoglycemic actions.32<\/sup>\u00a0Zingerone, gingerol & 6-shagoal were the major components determined in ginger. Ethanol & n-hexane extracts of ginger exhibits antibacterial activities against aerobic bacterias associated with periodontitis.33,34\u00a0<\/sup>Moreover it was found that the ethanolic extract was effective against Candida albicans and E. faecalis<\/em>.<\/p>\n

The efficacy of ethanolic extract of ginger was compared with chlorhexidine and garlic extract in a study, in that ginger exhibited highest antibacterial efficacy against E. faecalis<\/em>. 31<\/sup>\u00a0From the reported literature, ginger produced markable antimicrobial effect against gram negative bacterias like Porphyromonas gingivalis, Porphyromonas endodontalis <\/em>and Prevotella intermedia.<\/em> This is because that gingerol present in ginger inhibited the growth of these organisms.35<\/sup><\/p>\n

Jeeryin\u00a0<\/strong><\/p>\n

This is a Chinese herbal plant which were popular for its antimicrobial properties. It is effective against anerobes when used as irrigating solution along with ultrasonic activation. The 30% jieeryin solution has similar effect as that of NaOCl in root canal irrigation.36<\/sup><\/p>\n

Hybanthus enneaspermus<\/em><\/strong><\/p>\n

It is popularly called as Ratanpurus that belongs to Violaceae family, is herb or under shrub seen in tropical and sub-tropical areas. It has anti-inflammatory and anti-oxidant properties.37-41<\/sup><\/p>\n

In a study done by Kalepu Vamsi et al in 2014, to evaluate the antibacterial activity of Hybanthus enneaspermus<\/em> & CHX against E. faecalis,<\/em> the study results showed that 50%\u00a0 aqueous form of Hybanthus enneaspermus<\/em> inhibits the most resistant anaerobe E. faecalis<\/em> and its effect is as comparable with that of CHX.42<\/sup><\/p>\n

Herbal Alternatives Used As Intracanal Medicaments In Endodontics<\/strong><\/p>\n

Propolis<\/em><\/strong> \u2013 Honey bee wax<\/strong><\/p>\n

Propolis is a natural antibiotic resinous material obtained by honey bees (Apis mellifera L<\/em>.) from different plant species like trees of poplars & conifers and mix with wax and other substances. It exhibits varying degree of antioxidant, antiviral, anti-inflammatory , antibacterial, antifungal, antitumor, immunomodulating, pharmacological properties such as healing, cytostatic & are cariostatic. The main biologically active components present in this antitoxic natural substance are flavonoids and cinnamic acid derivatives.43, 44<\/sup><\/p>\n

In a study done with propolis, it inhibited the activity of hyaluronidase and has significant anti-inflammatory action as they contain caffeic acid and phenethylester (CAPE).45\u00a0<\/sup> Ethanolic preparation of propolis enhances bone regeneration and induces hard tissue bridge formation in vital pulp therapy procedures. It also contains viscidone which further makes it used as an irrigating solution. It can also be used as a transport medium for avulsed teeth as it maintains the viability of periodontal ligament cells. Propolis can be effectively used along with calcium hydroxide as dressing for elimination endodontic pathogens especially against E.faecalis<\/em>.46, 47<\/sup><\/p>\n

Aloe barbadenis<\/em><\/strong> \u2013 Aloe vera<\/strong><\/p>\n

Aloe vera contains clear gel surrounded by the green part of leaf. The two active components of aloe vera are aloin and aloe-emodin. Its antibacterial action is due to protein synthesis in the bacterial cells and it is rich in vitamins, minerals, enzymes, sugars, lignin, saponins, salicyclic acids and aminoacids. 48<\/sup><\/p>\n

It is effective against S. pyogenes <\/em>and E. faecalis<\/em> due to the presence of anthrax quinine which inhibits the formation of these two organisms. In an in vitro study done by Kurian B et al in 2016 showed MIC (Minimal Inhibitory Concentration) of Aloe vera was superior to calcium hydroxide in eliminating E. faecalis\u00a0 <\/em>and its antibacterial activity increased with time period.49\u00a0<\/sup>Compared to other natural extracts, Aloe vera has broad spectrum antibacterial activity against various oral pathogens.<\/p>\n

Agaricus bisporus \u2013 <\/em><\/strong>Mushroom\u00a0<\/strong><\/p>\n

It has both low and high molecular weight (LMW, HMW) active compounds. Because of these compounds, it possess medicinal properties like immune modulatory, anti-inflammatory, anti-viral, anti-oxidant and antimicrobial properties. The low molecular weight components present in mushroom are plectasin, confuentin, grifolin and neogrifolin which imparts capacity to penetrate deep into dentinal tubules.50<\/sup><\/p>\n

The gel form of mushroom is used as intracanal medicament. This is prepared by sun drying, grinding and boiling it with distilled water which then followed by adding hydroxyl ethyl cellulose as thickening agent in 2:1 ratio and injected into the canal with a syringe. Also it has been revealed that the gel form of the extract increases the contact time which enhances its performance. It has highest efficacy against gram negative bacterias. [49]<\/sup><\/p>\n

Arctium lappa – Burdock<\/em><\/strong><\/p>\n

This plant has been obtained from Japan and grown mostly in Brazil. They has many therapeutic applications. It possess antibacterial, antifungal, antiplatelet, antioxidant, diuretic, anxiolytic and HIV inhibitory effect. Artium lappa<\/em> contains sterols, tannins, sulphur containing polyacetylene, volatile fatty oils & polysaccharides. The active constituent of Burdock are sesquiterpene lactones and carbohydrate inulin. 51<\/sup><\/p>\n

The antimicrobial properties of Arctium lappa<\/em> was studied. From that it was concluded that the constituents of Arctium lappa<\/em> showed a great effect against the most organisms such as E. faecalis, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis <\/em>& Candida albicans. <\/em>This antimicrobial potential of the Arctium lappa<\/em> makes it use as root canal medicament.\u00a052<\/sup><\/p>\n

Glycyrrhiza glabra<\/em><\/strong> \u2013 Liquorice\u00a0<\/strong><\/p>\n

They are frequently used \u00a0Kampo medicines. It exhibits anti-inflammatory, anti-viral & anti-carcinogenic effects. It consists of a triterpenoid compound namely Glycyrrhizin that that imparts sweet taste to the liquorice root.53,54\u00a0<\/sup>Because of this Glycyrrhizin, it possess antimicrobial effect especially against E. faecalis. <\/em>55,56<\/sup><\/p>\n

Liquorice also inhibits most of the cariogenic bacterias like \u00a0Streptococcus mutans<\/em>.57<\/sup>\u00a0Its biocompatibility is compared to that of calcium hydroxide due to the presence of pentacyclic triterpenoid structure. But it has slightly acidic pH whereas calcium hydroxide having strong alkaline pH of 12. 58<\/sup><\/p>\n

Rhus plants<\/strong><\/p>\n

Rhus plants are enriched with tannins & gallic acids. Tannins exhibited antibacterial & antifungal properties. Gallic acid aids in healing periradicular inflammation. It also helps in opening up of obliterated dentinal tubules. [36]<\/sup><\/p>\n

Nissin<\/strong><\/p>\n

It\u2019s a naturally occurring antimicrobial peptide obtained from Streptococcus lactis<\/em> sub species. It exerts antimicrobial activity against a huge range of bacterial spores. It was also shown that it is potential in erradicatiion of E.faecalis<\/em> and superior to calcium hydroxide.59<\/sup><\/p>\n

Mahendra M et al in 2016 done a study to assess the efficacy of \u00a0Nissin, neem, propolis, calcium hydroxide & PRP when used as intracanal medicament against E. faecalis<\/em>. The results showed that Nissin produced better results than other tested agents.60<\/sup><\/p>\n

Citrus limonum – <\/em><\/strong>Lemon solution\u00a0<\/strong><\/p>\n

Lemon solution has pH of 2.21 with lower acidity. It is effective in clearing the smear layer thereby acting as a chelating agent. Fresh lemon solution has antibacterial property making it an ideal root canal medicament without any side effects.<\/p>\n

Abuziad & Eissa et al done a study, in which fresh lemon solution was shown to have wide antibacterial efficiency against E. faecalis<\/em> and hence can be used as an intracanal medicament.61<\/sup><\/p>\n

Vaccinium macrocarpon \u2013 <\/em><\/strong>Cranberry\u00a0<\/strong><\/p>\n

In ancient times, cranberry was used to treat stomach aches, scurvey and other liver diseases.62<\/sup>\u00a0They has numerous biologically active ingredients such as flavonoids, phenols, anthocyanins and condensed \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/strong>tannins.<\/p>\n

They have antibacterial, antimicrobial and antiadhesive properties, thereby prevents inhibition of many pathogens and biofilms. The compounds present in cranberry prevents acid formation & reduces the incidence of dental caries. As cranberry juice is naturally very acidic, it may cause erosion of the teeth if used too often which can lead to pain & sensitivity in the teeth.63<\/sup><\/p>\n

Papaine<\/strong><\/p>\n

Papaine is a proteolytic cysteine enzyme that comes from the latex of the leaves & fruits of the green adult papaya. It exhibits significant bacteriostatic and bactericidal effects.<\/p>\n

In the study by Anug Bhardwaj et al, antimicrobial efficacy of natural extracts of M.citrifolia<\/em>, papine, A.vera gel<\/em>, 2% CHX and calcium hydroxide against E.faecalis<\/em> were compared. In that Papaine produced significant results as compared to that of CHX than other tested agents.64<\/sup><\/p>\n

Eucalyptus globulus<\/em><\/strong> \u2013 Eucalyptus\u00a0<\/strong><\/p>\n

It is an essential oil obtained from the leaf of Eucalyptus tree. It has anti-inflammatory and antibacterial activities which can be used as a vehicle for intracanal medicaments.65\u00a0<\/sup>Studies have reported that Eucalyptus oil in pure concentration were effective on Pseudomonas aeroginosa<\/em> and Escherichia coli. <\/em>Considering the antimicrobial potential of eucalyptus essential oil against resistant microorganisms, it is expected that its association with calcium hydroxide contributes to the control of endodontic infections.66,67<\/sup><\/p>\n

Psidium guajava<\/em><\/strong> – Guava\u00a0<\/strong><\/p>\n

The leaves of guava are rich in guajaverin that inhibits S.mutans & Staphylococcus aureus formation that lead to plaque. They also contain phenolic compounds, vitamins, sesquiterpene alcohols, lineol, tannins, tripentenes & flavonoids. These all ingredients make them to exert anti-inflammatory, antimicrobial, antioxidant & antimutagenic properties. The ethanolic extract of it has higher antimicrobial activity.68<\/sup><\/p>\n

Riccinus communis<\/em><\/strong> – Castor<\/strong><\/p>\n

It is rich in castor acid or ricinoleic acid. It can be used as intracanal medicament as well as an irrigant too.69<\/sup>\u00a0In an in vitro study conducted by Marcio Carneiro Valera et al it was found that castor was able to destroy Candida albicans<\/em> and also significantly reduces the amount of E. faecalis.<\/em> 70<\/sup>\u00a0In other study done by Lucas da Fonseca Roberti Garcia et al, it was observed that calcium hydroxide and Castor oil paste had better activity than calcium hydroxide and propylene glycol paste against microorganisms commonly found in endodontic infections. 71<\/sup><\/p>\n

Carvacol<\/strong><\/p>\n

Carvacrol is extracted from Origanum vulgare. The prime use of carvacrol is inhibition of E. coli & Pseudomonas aeruginosa.<\/em> The main action of Carvacrol is disruption of bacterial cell membrane. It also aids in healing of periapical tissues as they stimulates the fibres of the pulp.72,73<\/sup><\/p>\n

It is commercially marketed by various companies like Sigma Aldrich, Biocore, MP Biochemicals, Life chemicals & Glentham Life Sciences Ltd.<\/p>\n

Conclusion:<\/strong><\/p>\n

Herbal formulations are advantageous in many ways. They are safe, easily available, increased shelf life, economic and mainly there is lack of microbial resistance so far. They are most effective if used with proper knowledge. Many studies have concluded that herbal extracts produced promising results when used as endodontic irrigant and intracanal medicament.<\/p>\n

References<\/strong><\/p>\n

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