Impact of Immunogen Structure to Specificity of Fluoroquinolones Detection by Microplate and Lateral Flow Immunoassay Techniques

Nadezhda A. Taranova; Elena A. Zvereva; Anatoly V. Zherdev; Boris B. Dzantiev; Iliya A. Shanin; Sergey A. Eremin

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Impact of Immunogen Structure to Specificity of Fluoroquinolones Detection by Microplate and Lateral Flow Immunoassay Techniques

Volume 8, Number 2

Nadezhda A. Taranova¹, Elena A. Zvereva¹, Anatoly V. Zherdev¹, Boris B. Dzantiev^1*, Iliya A. Shanin², Sergey A. Eremin²

¹A. N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 33 Leninsky prospect, Moscow 119071, Russia. ²M. V. Lomonosov Moscow State University, Chemical Department, Leninskie Gory, 119991 Moscow, Russia.

DOI : http://dx.doi.org/10.13005/bpj/899

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ABSTRACT:

When immunodetection of structurally related compounds important to be able to vary the selectivity of their immune recognition that, depending on the task, to identify well-defined or the total content of the compounds. The selection of immunogenic fluoroquinolone derivatives and a method for fluoroquinolone conjugation with a carrier protein allowed for modulating the specificity of the produced antibodies. We used different derivatives to produce polyclonal antibodies and studied their specificity in microplate immunoenzyme and lateral flow immunochromatographic assays. The use of hapten with free variable part for the production of antibodies allowed the detection of levofloxacin, its chiral isomer ofloxacin, and structurally related marbofloxacin. The modified hapten with free constant part allows us to develop analytical systems specific to ciprofloxacin that also have broad specificity for eight other fluoroquinolones. Comparison of the data of ELISA and LFTS showed a fundamental similarity in recognizable derivatives. However, a measure of cross-reactivity was matched.

KEYWORDS:

Fluoroquinolones; ELISA; Immunochromatographic assays; Colloidal gold; immunogen structure