Study on the Apoptotic Properties of Methanolic Extracts of Peltophorum Pterocarpum, Cassia Auriculata, Cassia Alata and Lamprachaenium Microcephalum
M. S. Mohamed Jaabir*, A. Mansoor Hussain, T. Shariq Afsar and S. Senthil KumarDepartment of Biotechnology, Jamal Mohamed College (Autonomous), Tiruchirappalli - 620 020 India.
Abstract: Peltophorum pterocarpum (Tam: Malai porasu), Cassia auriculata (Tam: Avaram), Cassia alata (Tam: Seemaiaganthi) and Lamprachaenium microcephalum (Tam: Brahmadandi) were chosen for the study to induce apoptosis. The methanolic extracts of the leaves were dried and dissolved in DMSO in concentration of 100mg/mL for treating MCF-7 (Breast cancer) cell line grown in RPMI- C medium with 10% Foetal Bovine Serum at pH 7.1, temperature 370C and 5% CO2. The cytotoxicity of extracts on MCF-7 was determined by MTT Assay and found to be as 3mg/ml for Peltophorum pterocarpum and Cassia auriculata and 1mg/ml for Lamprachaenium microcephalum and Cassia alata. The sublethal concentrations of all the four extracts were treated and the morphology of cells after AOEB/Hoechst 33258 staining was visualized under Epifluorescence. Cells treated with Lamprachaenium microcephalum and Cassia alata showed resemblance to Apoptotic death while that of Peltophorum pterocarpum and Cassia auriculata resembled much of Necrosis.
Keywords: Peltophorum pterocarpum; Cassia auriculata; Cassia alata; Lamprachaenium microcephalum; MTT Assay Back to TOC