Investigating on Effect of Wormwood Extract on Reduction of Renal Toxicity in Treated Rats by Azathioprine
Farokhnia Farzaneh*, Hosseini Seyed Ebrahim, Vahdati AkbarDepartment of Biology, Science and Research Branch, Islamic Azad University, Fars, Iran. Corresponding Author: Email: Farokhnia1372@gmail.com
Abstract: azathioprine is a chemical drug that is used for treatment of diseases with immunological origin. Studies have proven that the drug has potential side effects on the renal tissues. So finding a suitable antioxidant to reduce the pathological effects of azathioprine drug on kidney is the objective of the present study. Methods: the study was conducted on 42 mature female rats. After weighing, the rats were divided into 6 groups of 7 each, which includes control (without any treatment), blank (received 5 mg ofnormal saline), experimental group1 (azathioprine (50mg/kg) were administered intraperitoneally), experimental group2 (received azathioprine (50mg/kg) intraperitoneally and orally received wormwood extract (125mg/kg)), experimental group3 (received azathioprine (50mg/kg) intraperitoneally and orally received wormwood extract (250mg/kg)), experimental group4 (received azathioprine (50mg/kg) intraperitoneally and orally received wormwood extract (500mg/kg)). After 28 days, the rats of all groups became unconscious and their renal tissues were isolated for histological studies. Results: the results showed that reduction of glomerular and collecting ducts diameter, as well as malpighian body diameter were observed at the level of 5% in the experimental group1 in compared to control. The collecting ducts diameter in the experimental group3,which had received the maximum dose of the extract,increased compared to the experimental group 1. Conclusion: the obtained results of the study show that azathioprine drug causes impairment in renal tissues and wormwood extract due to its antioxidant properties, reduces the pathological effects of azathioprine.
Keywords: Wormwood; Histopathologic; Kidney; Azathioprine; Rat Back to TOC