Novel Spectroscopic Methods for Determination of Folic Acid in Pharmaceutical Formulations
V. S. Settaluri, C. Shechinah Felice*, Spandana Vemulapally and Spandana ValluruDepartment of Biotechnology, K.L.University, Vaddeswaranm Guntur- 522 502 India.
Corresponding Author E-mail:shechinahfelice@yahoo.co.in
Abstract: Sophisticated analytical methods viz. HPLC and HPTLC which are being employed for analysis are relatively expensive and hence need for simple analytical methods arises, that are suggested in the proposed methods for routine determination of Folic acid in pharmaceutical formulations and bulk dosage forms. These methods are based on the formation of colored species on binding of Folic acid with Sodium nitroprusside and Ammonia reagent to produce a dark yellow colored chromogen (lmax at 390) For Method A and MBTH and ferric chloride to produce a green colored chromogen (lmax at 690) for Method B. Results of analysis were validated statistically and by recovery studies. Assay and recovery studies were also performed. The percent Relative Standard Deviation of these methods were found to be 1.37 for Method A and 0.77 for Method B. Based on these values these methods could be treated as simple, sensitive and reproducible based on the principle of absorption visible spectrophotmetry for the determination of Folic acid in pharmaceutical formulations.
Keywords: Folic acid; Analysis; Spectroscopy; Molar abosrptivity; Beer’s Law Back to TOC