Triolein from Coix Lacryma-Jobi Induces Cell Cycle Arrest through P53/P21Signaling Pathway
Tran Thi Hien1,5*, Do Thi Ha3, Do Minh Truong2, Loi Vu Duc4, Trong Tuan Dao3 and Nguyen Thanh Hai41Department of pharmacy, Thai Binh medical and pharmacy University, Vietnam.
2College of Sciences, Thai Nguyen University, Vietnam.
3National Institute of Medicinal Materials, 3B Quangtrung, Hanoi, Vietnam.
4School of Medicine and Pharmacy, Hanoi National University, 144 Xuan Thuy street, Cau Giay, Hanoi, Vietnam.
5Faculty of medicine, Lund University, Sweden.
*Corresponding Author E-mail: nguyenthanh.haihnu@gmail.com
Abstract: p53, a tumor suppressor protein, has important roles in DNA repair, cell cycle and apoptosis, is a one of the key events in cancer development. Coix lacryma-jobi seed has been used as a food and traditional medicine plant with anti-oxidant, anti-cancer and anti-diabetic effects. In currently research, we identified the most potent p53-increasing compound among 4 compounds (1 – 4) found in Coix lacryma-jobi and demonstrated its molecular mechanism in MCF-7 cells. Among the four isolated compounds (1 – 4), triolein most increased p53. Triolein treatment induced p53, p21, p27 and Bax in MCF-7 cells. Moreover, triolein caused S phase arrest through suppression of CDK1, phopho-Rb and E2F1 in dose-dependent manner. We also observed the decreasing of DNA synthesis by triolein. These data suggest that triolein may induced cell cycle restart involve DNA synthesis and apoptosis pathway in MCF-7 cells.
Keywords: Triolein; Coix lacryma-jobi; MCF-7 cells; apoptosis; DNA synthesis Back to TOC