Release of Cytochrome c in MCF-7 Cells Treated With 7, 3’, 5’-Trihydroxyflavanone of Hydnophytum Formicarium
A. Hasmah 1*, Azimahtol Hawariah L. P.2 and Judith Hohmann31School of Health Sciences, Health Campus, Universiti Sains Malaysia, 16150 Kota Bharu, Kelantan, Malaysia. ²School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor, Malaysia ³Institute of Pharmacognosy, University of Szeged, Hungary. Corresponding Author E-mail: hasmah@kck.usm.my
Abstract: Cytochrome c is a central protein that plays a role in triggering apoptosis in cell induced by any agent either drug or plant extracts. Here, we present data of an active antiproliferative agent 7,3’,5’-trihydroxyflavanone (3HFD) that is elucidated from the plant Hydnophytum formicarium showed to trigger cytochrome c release in treated MCF-7 cell. By performing Western Blot, cytochrome c was seen to be elevated throughout the experiments. The increase level of cytochrome c was confirmed by ELISA and remarked as the beginning of the caspase cascade without altering caspase-8 level. In conclusion, the 3HFD seems to significantly induced apoptosis via mitochondria pathway as reflected by up regulation of cytochrome c.
Keywords: Cytochrome c; MCF-7 cels; Hydnophytum formicarium Back to TOC