Anti-Inflammatory and Antioxidant Properties of the Mucuna sanjappae Seeds in the Rat Model and In Vitro Assays

Ravishankar Patil^1,2,4,5, Chetan Aware¹, Kavita Shinde³, Ruchika Koul-Ghanekar³, Govind vyavahare¹, Vishwas Bapat² and Jyoti Jadhav^1,2*   

¹Department of Biotechnology, Shivaji University, Vidyanagar, Kolhapur, Maharashtra, India.

²Department of Biochemistry, Shivaji University, Vidyanagar, Kolhapur, Maharashtra, India.

³Interactive Research School for Health Affairs (IRSHA), Bharati Vidyapeeth University, Pune, Maharashtra, India.

⁴Amity Institute of Biotechnology, Amity University, Mumbai, Maharashtra, India.

⁵Amity Centre for Nuclear Biotechnology, Amity University, Mumbai, Maharashtra, India

Corresponding Author E-mail:biotechjpj@gmail.com

Abstract: The Fabaceae (Leguminosae) plant family contains several species of the Mucuna Adans. genus possessing therapeutic potential and growing widely in tropical and sub-tropical regions. In this research, we investigated the anti-inflammatory and antioxidant properties of the extract from the Mucuna sanjappae Aitawade & S.R.Yadav seeds. Initially, we conducted an in vitro anti-inflammatory activity test using the bovine serum albumin anti-denaturation assay and found promising dose-dependent activity. Subsequently, we performed an in vivo anti-inflammatory and antioxidant study on a rat paw edema model induced by carrageenan. Three different doses of M. sanjappae seed water extract (50, 100 and 200mg/kg B/W) were used for the study (Oral administration). Edema measurement was carried out at 0, 2, 4 and 6 hr intervals. Dose dependent inhibition in edema in the M. sanjappae seed extract treatment group was observed with maximum activity for 200mg/kg B/W dose at 4 hr (53.49%). Standard drug showed maximum edema inhibition (54.94%) at 6hr. Our results also showed that, M. sanjappae seed extract inhibited pro-inflammatory cytokine TNF-α and increases anti-inflammatory cytokine IL-10 with increased level of blood serum antioxidants. Phytochemical analysis for secondary metabolites including polyphenol, flavonoids, phytic acid, proanthocyanidin, tannin and saponin was also quantified which might be the responsible component for biological activities under study.

Keywords: Antioxidant; Anti-inflammatory drug; Carrageenan; Cytokine; Inflammation; Mucuna

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