Phytoconstituents Analysis and Anti-Diabetic Potential of Sembung Leaf Extract (Blumea balsamifera L. DC.) through Inhibition of NF-KB p65, GLP-1, and DPP-4 Proteins with In-Silico Approaches
Ni Putu Widya Astuti1,2*
, Ida Bagus Putra Manuaba3, I Made Jawi4, Anak Agung Bawa Putra3, Putu Angga Wiradana5, I Gede Widhiantara5, Anak Agung Ayu Putri Permatasari5, Arif Nur Muhammad Ansori6, and Viol Dhea Kharisma71Doctoral Study Program, Faculty of Medicine, Universitas Udayana, Denpasar City, Bali Province (80232), Indonesia
2Study Program of Public Health, Faculty of Health, Science, and Technology, Universitas Dhyana Pura, Jalan Raya Padangluwih, Dalung, North Kuta, Badung Regency, Bali Province (80351), Indonesia
3Department of Chemistry, Faculty of Mathematical and Natural Sciences, Universitas Udayana, Badung Regency, Bali Province (80361), Indonesia
4Department of Pharmacology, Faculty of Medicine, Universitas Udayana, Jalan P.B. Sudirman, Dangin Puri Klod, Denpasar City, Bali Province (80232), Indonesia
5Research Group of Biological Health, Study Program of Biology, Faculty of Health, Science, and Technology, Universitas Dhyana Pura, Jalan Raya Padangluwih, Dalung, North Kuta, Badung Regency, Bali Province (80351), Indonesia
6Postgraduate School, Universitas Airlangga, Kampus B, Jalan Airlangga, Surabaya, East Java (60286), Indonesia
7Study Program of Biology, Faculty of Science and Technology, Universitas Airlangga, Kampus C, Jln. Dr. Ir. H. Soekarno, Mulyorejo, Surabaya, East Java (60115), Indonesia
Corresponding Author E-mail: widyaastuti@undhirabali.ac.id
Abstract: Traditional herbal remedies have an important role in human health. Empirically, Blumea balsamifera is often used as a traditional beverage to alleviate fever symptoms, lower cholesterol levels, and maintain body immunity. The purpose of this study was to discover the phytoconstituent profile that contributes to the anti-diabetic properties of B. balsamifera leaf extract (BBLE) using in silico approaches.LCMS/MS was used to identify the constituent profile of BBLE, and the ability of these compounds against diabetes-related proteins was analyzed computationally.Three proteins related to diabetes are NF-KB p65, GLP-1, and DPP-4. A total of 18 compounds were successfully identified through LCMS/MS, including 4 compounds known to be flavonoid derivatives and can be used as markers of BBLE. Pheophorbide A and 1,1-Cyclopentanediacetic acid were reported for the first time to inhibit the NF-KB p65, GLP-1, and DPP-4 proteins in docking simulation studies. Based on these findings, it can be confirmed that the bioactive compounds in BBLE show strong inhibitory potential against anti-diabetic proteins.
Keywords: Antidiabetic activity; Blumea balsamifera; In Silico; LCMS/MS; Phytoconstituent Back to TOC