UPLC-ESI-MS\MS Phytochemicals Profiling of n-Butanol Chloroform and Hexane Fraction of Xanthium strumarium Fruit Extract
Mohammed A. Ezghayer 1*
, Omar Hussein Ahmed 2Â and Mostafa F. Tawfeeq 31Pharmacognosy Department, faculty of pharmacy, Tikrit university, Tikrit, Iraq.
2Pharmaceutical chemistry Department, faculty of pharmacy, Tikrit university, Tikrit, Iraq.
Corresponding Author E-mail: moh.cognosy@tu.edu.iq
Abstract: The work aimed to explore the phytochemicals of various fractions of Xanthium strumarium fruit extract since this plant is found to be toxic for both humans and animals in Iraq. The extracts underwent phytochemical screening, which indicated the occurrence of flavonoids, alkaloids, phenols, coumarin, saponin, tannins, and terpenoids. After extraction, fractionation was done using hexane, chloroform, and N-butanol respectively, for identifying the phytochemicals found in each fraction, UPLC-electrospray ionization–tandem mass spectroscopy was used which revealed the presence of 6 sterol and terpenes compound in hexane fraction, Nine compounds were detected in the chloroform fraction, among which 2-acetyl-atractyligenin and Artemisinin were the primary components. Additionally, this particular plant has been found to contain these compounds for the first time. Furthermore, Pungiolide C, dihydroartemisinin, and atractylenolide II were also detected for the first time in this plant. For the n-Butanol fraction, eight diterpene glycosides were recognized, with Atractyloside and Carboxyatractyloside and their desulphated derivatives as the major compounds responsible for the plant's toxicity. The butanol fraction also showed the presence of eight phenolic compounds, among which caffeoylquinic acid derivatives and flavonoid Syringetin were the major compounds in this fraction.
Keywords: Artemisinin; Carboxyatractyloside; UPLC-ESI-MS/MS; Xanthium strumarium Back to TOC