Chromatographic Profile of Polyphenols in the Agastache foeniculum (Pursh) Kuntze Herb: Evaluation of Optimal Extraction Efficiency
Mariia Shanaida1*
, Olesia Palamar1 and Olena Holembiovska21Department of Pharmacognosy and Medical Botany, I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine.
2Department of Translational Medical Bioengineering, National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute”, v, Ukraine.
Corresponding Author E-mail: shanayda@tdmu.edu.ua
Abstract: During its triple extraction, the HPLC analysis revealed polyphenols' contents in the A. foeniculum herb. Several hydroxycinnamic acids (rosmarinic, chlorogenic, ferulic and caffeic) and flavonoids (apigenin, apigenin-7-O-glucoside, hyperoside, quercitrin, rutin and quercetin) were identified in the A. foeniculum herb. It was established that rosmarinic acid followed by apigenin-7-O-glucoside and apigenin were the predominant compounds of the A. foeniculum raw material. The content of rosmarinic acid as the major compound during the primary, secondary and tertiary extraction decreased in the following order: 37.563>15.435>0.642 (mg/g); the content of apigenin-7-O-glucoside was 24.508>9.107>0.945 (mg/g) and apigenin was 19.547>9.676>1.816 (mg/g), respectively. Generally, the third extraction was determined to be inefficient in terms terms of low content of polyphenols as well as excessive analysis time and solvent costs.
Keywords: Aerial part; Anise hyssop; High-performance liquid chromatography; Multiplicity of extraction; Polyphenols Back to TOC